>KMC003033A_C01 KMC003033A_c01
caactttCAACCAAAATATTTTTTTATATATTTCTTTTTTTTCGATACATCGGAAAGCTA
ACAAAACAGAGAAAAGGACTACAAGGCACTTAAAGAGTCTTTCATAAGGAGGACTTGTAA
ATCAGGATCTGGTTCTCGTAGGAGACGAGTGACCAAGCTGAACGCGGAGCTGATTTAGCC
ATGAAATCTGCGACGTAGTTCCCCTCACGAGGGATTGCTCGAACCGTAACCACCCACTGT
CTCCTGAGCAGAAGTCTGCACTCCAAAACCTCCGGTACAAAGCATTTTTTTATATATTTC
AGAAGAAAAAAAACATTTTTTGCCCCACACTCTCATAGTCTCATTTTCTATCTATATCCT
GATGCCAAATTCACGTGCTGCTCATCAATAAATAAACCCCACAAGTCCCCTTTCAAAAAG
GAAAAATATCGTGGAATTTTTTTCCTTCGGTTATTATTAATTTAAGTAAACAAAAACCAA
CAAGAAAAAAATAAGTTTATCATCATCGGAGGAACAATCCACCTCAGGGATGAACCGGTT
CAGGGCGTTCATCCGGCACCCCCACAACCTTTTTTTACACGTGAAATTGGCACGGACGAA
CAATGGAACAACATGCATCACCAGTGCCTTCCCTGTGAACTACCTCCTCCTTGGTTCTTT
CCAAAGAAGGTGTTCTTGGATTTTGACTTTGGTAACTCATGGATATCCATCACCTGAATC
GTTCTTTGCTTTTTGGCATTTTCAGCATCTCTGTAGTTCTCTTGAAGCCGTTTTCTTGCC
GAAGCAAGCTTCTCCGCCTCAAAATTGCTATTCCTCTGTTCTCTTTGCTTATTTTGAGGA
GTAAATGCAAGAGTGGTAACTGATGGTGATGGCGTTGGTTTTGGCGGAGTTTGGCGAGGA
ATCACTTTCGGTTTAGGTTCTGCTTCTGAGTGGTTGCGGTCAGACCCAGAGCTCCCATTG
TGTGGATTTGGCGAGTACGCAAAA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003033A_C01 KMC003033A_c01
         (984 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568218.1| putative protein; protein id: At5g09850.1, supp...   103  4e-21
dbj|BAB09406.1| gb|AAF19567.1~gene_id:MYH9.6~similar to unknown ...   103  4e-21
gb|AAL25557.1| AT5g09850/MYH9_6 [Arabidopsis thaliana] gi|201472...   103  4e-21
gb|AAM08816.1|AC090486_26 Hypothetical protein [Oryza sativa] gi...    54  3e-06
dbj|BAC16486.1| contains ESTs AU092606(C52561),AU063213(C53672),...    54  5e-06

>ref|NP_568218.1| putative protein; protein id: At5g09850.1, supported by cDNA:
           gi_16648729 [Arabidopsis thaliana]
          Length = 353

 Score =  103 bits (257), Expect = 4e-21
 Identities = 63/130 (48%), Positives = 80/130 (61%), Gaps = 9/130 (6%)
 Frame = -2

Query: 983 FAYSPNPHNGSSGSDRNHSEAEP--KPKVI---PRQTPPKPT----PSPSVTTLAFTPQN 831
           F YSP P NG S S +N +  EP  KP+ +   PR+  P P     PSPS  T+      
Sbjct: 236 FGYSPVPQNGYSSSSKNSNITEPERKPRPVAPQPRRESPSPAKPSRPSPSQQTIP----- 290

Query: 830 KQREQRNSNFEAEKLASARKRLQENYRDAENAKKQRTIQVMDIHELPKSKSKNTFFGKNQ 651
           + +E +  +F+     +ARKRLQ+NYR AENAKKQRTIQVMDIH++PK K K  FF + +
Sbjct: 291 RDKEHKEVDFD-----TARKRLQQNYRQAENAKKQRTIQVMDIHDIPKPK-KGGFFPR-K 343

Query: 650 GGGSSQGRHW 621
           GG S  GRHW
Sbjct: 344 GGSSQGGRHW 353

>dbj|BAB09406.1| gb|AAF19567.1~gene_id:MYH9.6~similar to unknown protein
           [Arabidopsis thaliana]
          Length = 361

 Score =  103 bits (257), Expect = 4e-21
 Identities = 63/130 (48%), Positives = 80/130 (61%), Gaps = 9/130 (6%)
 Frame = -2

Query: 983 FAYSPNPHNGSSGSDRNHSEAEP--KPKVI---PRQTPPKPT----PSPSVTTLAFTPQN 831
           F YSP P NG S S +N +  EP  KP+ +   PR+  P P     PSPS  T+      
Sbjct: 244 FGYSPVPQNGYSSSSKNSNITEPERKPRPVAPQPRRESPSPAKPSRPSPSQQTIP----- 298

Query: 830 KQREQRNSNFEAEKLASARKRLQENYRDAENAKKQRTIQVMDIHELPKSKSKNTFFGKNQ 651
           + +E +  +F+     +ARKRLQ+NYR AENAKKQRTIQVMDIH++PK K K  FF + +
Sbjct: 299 RDKEHKEVDFD-----TARKRLQQNYRQAENAKKQRTIQVMDIHDIPKPK-KGGFFPR-K 351

Query: 650 GGGSSQGRHW 621
           GG S  GRHW
Sbjct: 352 GGSSQGGRHW 361

>gb|AAL25557.1| AT5g09850/MYH9_6 [Arabidopsis thaliana] gi|20147241|gb|AAM10334.1|
           AT5g09850/MYH9_6 [Arabidopsis thaliana]
          Length = 302

 Score =  103 bits (257), Expect = 4e-21
 Identities = 63/130 (48%), Positives = 80/130 (61%), Gaps = 9/130 (6%)
 Frame = -2

Query: 983 FAYSPNPHNGSSGSDRNHSEAEP--KPKVI---PRQTPPKPT----PSPSVTTLAFTPQN 831
           F YSP P NG S S +N +  EP  KP+ +   PR+  P P     PSPS  T+      
Sbjct: 185 FGYSPVPQNGYSSSSKNSNITEPERKPRPVAPQPRRESPSPAKPSRPSPSQQTIP----- 239

Query: 830 KQREQRNSNFEAEKLASARKRLQENYRDAENAKKQRTIQVMDIHELPKSKSKNTFFGKNQ 651
           + +E +  +F+     +ARKRLQ+NYR AENAKKQRTIQVMDIH++PK K K  FF + +
Sbjct: 240 RDKEHKEVDFD-----TARKRLQQNYRQAENAKKQRTIQVMDIHDIPKPK-KGGFFPR-K 292

Query: 650 GGGSSQGRHW 621
           GG S  GRHW
Sbjct: 293 GGSSQGGRHW 302

>gb|AAM08816.1|AC090486_26 Hypothetical protein [Oryza sativa]
           gi|20303621|gb|AAM19048.1|AC099774_10 putative membrane
           spanning protein [Oryza sativa (japonica
           cultivar-group)]
          Length = 274

 Score = 54.3 bits (129), Expect = 3e-06
 Identities = 33/81 (40%), Positives = 44/81 (53%)
 Frame = -2

Query: 914 KPKVIPRQTPPKPTPSPSVTTLAFTPQNKQREQRNSNFEAEKLASARKRLQENYRDAENA 735
           K    P    P PT SP  T       + +RE+  +  +  KLA A+++LQE Y DA +A
Sbjct: 174 KANTPPAAQKPAPTASPKKTA------SNKREEAPALVDEAKLAVAKRKLQEGYEDAASA 227

Query: 734 KKQRTIQVMDIHELPKSKSKN 672
           KKQR IQV+D    P+ K KN
Sbjct: 228 KKQRMIQVID---APRKKVKN 245

>dbj|BAC16486.1| contains ESTs
           AU092606(C52561),AU063213(C53672),C27675(C52561)~unknown
           protein [Oryza sativa (japonica cultivar-group)]
           gi|23495719|dbj|BAC19931.1| contains ESTs
           AU092606(C52561),AU063213(C53672),C27675(C52561)~unknown
           protein [Oryza sativa (japonica cultivar-group)]
          Length = 435

 Score = 53.5 bits (127), Expect = 5e-06
 Identities = 34/105 (32%), Positives = 53/105 (50%), Gaps = 7/105 (6%)
 Frame = -2

Query: 965 PHNGSSGSDRNHSEAEPKPKVIPRQTPPKPTPSPSVTTLAFTPQNKQREQ----RNSNFE 798
           P N  SG  R     +      P Q   +P PS    + +   +N  R +    +N+  E
Sbjct: 312 PSNPESGPGRPQPRTKQHQDASPAQGRSQPMPSDK--SASHHDENSVRAKLELAKNAKLE 369

Query: 797 ---AEKLASARKRLQENYRDAENAKKQRTIQVMDIHELPKSKSKN 672
              + KL   +++LQE Y++ +NAKKQRTIQ++D   LPK  ++N
Sbjct: 370 LTNSAKLEVTKRKLQEGYQEFDNAKKQRTIQMVDPQNLPKQANRN 414

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 899,696,099
Number of Sequences: 1393205
Number of extensions: 21545027
Number of successful extensions: 86488
Number of sequences better than 10.0: 117
Number of HSP's better than 10.0 without gapping: 70674
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 84346
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 56574306528
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF045h09_f BP030696 1 467 2 MR055h04_f BP080268 8 510 3 MWM163c03_f AV767257 8 419 4 MR085e09_f BP082551 9 396 5 MR016e01_f BP077189 34 414 6 GNf014a03 BP068349 43 282 7 MR064b07_f BP080891 289 808 8 MR008c08_f BP076544 431 826 9 MR093c11_f BP083146 434 891 10 GNf071b03 BP072598 436 967 11 GNf097a11 BP074530 445 812 12 MR042b09_f BP079231 447 910 13 MPDL003d09_f AV776672 447 1003 14 GNf094c01 BP074310 447 851 15 GNf040h11 BP070353 447 857 16 GNf046d06 BP070756 447 927 17 MR041a07_f BP079136 451 835

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