KMC003001A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC003001A_C01 KMC003001A_c01
gggtcgggccccccgttggatcaccacacgATTGAGTTTCAAATCAATTCATCTTCACTT
TGTGTTGGTGCTTCCTGGAATTACCATTTTGCCCCTGTCATTCCACTAACTTTGCCCCTT
AAAATCACATTGTTTTATCAATTCCACAAACAAACACAAAGCCTTCAATGGCGTCCATGG
CAGCAACAACCCTTTTCGTGAAAACCCCTTTCCCCTCTCATTCCCTGTCGAAATCATTCG
ATACCCATTTTTCTCCGTCGCTCAAAGTCAACGTCTTGACCGTCGCCAAGGCGAGGGGAG
TCGGGTCGGGTCTCCGGGTTTCAAACGGGTTGATCGAACCCGATGGTGGGAAGCTGGTGG
AGCTTCTGGTGAAGGATTCGGAGAGAGATTTGAAGAAGGGTGAGGCTCTGTCTCTTCCAA
GGATCAAGCTCTCTAAGATGGATGTGGAGTGGGTTCACGTTCTGAGTGAAGGATGGGCCA
CGCCACTTCAAGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTCAACTCAC
TGCGCCTTGATGATGGCACGGTGGTTAACATGTCTGTGCCGATCGTGCTTGCCATCGATG
ATGCGCAGAAGCACCGGATCGGTGATTCCAAGAAGGTTGCACTTTTTGATTCTCTGGGCA
ACCCTGTTGCCATTCTTAAAGATATTGAAATTTATAAGCACCCTAAAGAAG
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003001A_C01 KMC003001A_c01
(711 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL74418.1|AF452454_1 ATP sulfurylase [Glycine max] 280 1e-74
pir||S44267 sulfate adenylyltransferase (EC 2.7.7.4) met3-2 - po... 228 6e-59
ref|NP_188929.1| ATP sulfurylase, putative; protein id: At3g2289... 217 1e-55
gb|AAF19185.1|AF198964_1 ATP sulfurylase [Arabidopsis thaliana] 217 1e-55
gb|AAM63185.1| ATP sulfurylase, putative [Arabidopsis thaliana] 214 9e-55
>gb|AAL74418.1|AF452454_1 ATP sulfurylase [Glycine max]
Length = 461
Score = 280 bits (716), Expect = 1e-74
Identities = 141/174 (81%), Positives = 157/174 (90%)
Frame = +3
Query: 189 TLFVKTPFPSHSLSKSFDTHFSPSLKVNVLTVAKARGVGSGLRVSNGLIEPDGGKLVELL 368
T F +T FPSHSLSK+FDTHF+P+ KVNV +AR G+RVSN LIEPDGGKLVEL+
Sbjct: 3 TFFAQTSFPSHSLSKTFDTHFAPAPKVNVFVNFRARR-HVGVRVSNALIEPDGGKLVELV 61
Query: 369 VKDSERDLKKGEALSLPRIKLSKMDVEWVHVLSEGWATPLQGFMRESEFLQTLHFNSLRL 548
V D ERDLKKGEALSLPRIKLS++D+EWVHVLSEGWATPL+GFMRE+EFLQTLHFNSLRL
Sbjct: 62 VTDFERDLKKGEALSLPRIKLSRIDLEWVHVLSEGWATPLKGFMREAEFLQTLHFNSLRL 121
Query: 549 DDGTVVNMSVPIVLAIDDAQKHRIGDSKKVALFDSLGNPVAILKDIEIYKHPKE 710
DDG+VVNMSVPIVLAIDDAQKHRIGD+KKVALFDS G+PVAIL +IEIYKHPKE
Sbjct: 122 DDGSVVNMSVPIVLAIDDAQKHRIGDNKKVALFDSKGDPVAILNNIEIYKHPKE 175
>pir||S44267 sulfate adenylyltransferase (EC 2.7.7.4) met3-2 - potato
gi|479090|emb|CAA55655.1| sulfate adenylyltransferase
[Solanum tuberosum]
Length = 463
Score = 228 bits (581), Expect = 6e-59
Identities = 115/174 (66%), Positives = 139/174 (79%)
Frame = +3
Query: 189 TLFVKTPFPSHSLSKSFDTHFSPSLKVNVLTVAKARGVGSGLRVSNGLIEPDGGKLVELL 368
+LF+KTP PS SL K+ THF + + +K R + R+ GLIEPDGGKLVEL+
Sbjct: 3 SLFLKTPGPSQSLPKTHKTHFVLPQNLPLSWRSKYRAGPAAARIRCGLIEPDGGKLVELI 62
Query: 369 VKDSERDLKKGEALSLPRIKLSKMDVEWVHVLSEGWATPLQGFMRESEFLQTLHFNSLRL 548
V++ +RDLK+ +ALSLP+IKLSK+D++WVHVLSEGWA+PL+GFMRESEFLQTLHFNSLRL
Sbjct: 63 VEEPQRDLKRRQALSLPQIKLSKIDIQWVHVLSEGWASPLKGFMRESEFLQTLHFNSLRL 122
Query: 549 DDGTVVNMSVPIVLAIDDAQKHRIGDSKKVALFDSLGNPVAILKDIEIYKHPKE 710
DG+VVNMSVPIVLAIDD+ K+ IGDS VAL D NP+AIL DIEIYKH KE
Sbjct: 123 GDGSVVNMSVPIVLAIDDSNKNNIGDSSSVALVDDKDNPIAILNDIEIYKHNKE 176
>ref|NP_188929.1| ATP sulfurylase, putative; protein id: At3g22890.1, supported by
cDNA: 20342., supported by cDNA: gi_13877582, supported
by cDNA: gi_15292870, supported by cDNA: gi_15809803,
supported by cDNA: gi_17978730, supported by cDNA:
gi_20258904, supported by cDNA: gi_452469 [Arabidopsis
thaliana] gi|11994718|dbj|BAB03034.1| ATP
sulfurylase/APS kinase [Arabidopsis thaliana]
gi|13877583|gb|AAK43869.1|AF370492_1 ATP sulfurylase/APS
kinase [Arabidopsis thaliana] gi|15292871|gb|AAK92806.1|
putative ATP sulfurylase [Arabidopsis thaliana]
gi|15809804|gb|AAL06830.1| AT3g22890/F5N5_6 [Arabidopsis
thaliana] gi|17978731|gb|AAL47359.1| ATP sulfurylase/APS
kinase [Arabidopsis thaliana] gi|20258905|gb|AAM14146.1|
putative ATP sulfurylase [Arabidopsis thaliana]
gi|27311865|gb|AAO00898.1| Unknown protein [Arabidopsis
thaliana]
Length = 463
Score = 217 bits (552), Expect = 1e-55
Identities = 118/181 (65%), Positives = 140/181 (77%)
Frame = +3
Query: 168 MASMAATTLFVKTPFPSHSLSKSFDTHFSPSLKVNVLTVAKARGVGSGLRVSNGLIEPDG 347
MASMAA + KTPF S L+KS P V+ + + R VGS + GLI PDG
Sbjct: 1 MASMAA--VLSKTPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGS---IRAGLIAPDG 55
Query: 348 GKLVELLVKDSERDLKKGEALSLPRIKLSKMDVEWVHVLSEGWATPLQGFMRESEFLQTL 527
GKLVEL+V++ +R KK EA LPR++L+ +D++W+HVLSEGWA+PL GFMRESEFLQTL
Sbjct: 56 GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 115
Query: 528 HFNSLRLDDGTVVNMSVPIVLAIDDAQKHRIGDSKKVALFDSLGNPVAILKDIEIYKHPK 707
HFNSLRLDDG+VVNMSVPIVLAIDD QK RIG+S +VALF+S GNPVAIL DIEIYKHPK
Sbjct: 116 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPK 175
Query: 708 E 710
E
Sbjct: 176 E 176
>gb|AAF19185.1|AF198964_1 ATP sulfurylase [Arabidopsis thaliana]
Length = 463
Score = 217 bits (552), Expect = 1e-55
Identities = 118/181 (65%), Positives = 140/181 (77%)
Frame = +3
Query: 168 MASMAATTLFVKTPFPSHSLSKSFDTHFSPSLKVNVLTVAKARGVGSGLRVSNGLIEPDG 347
MASMAA + KTPF S L+KS P V+ + + R VGS + GLI PDG
Sbjct: 1 MASMAA--VLSKTPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGS---IRAGLIAPDG 55
Query: 348 GKLVELLVKDSERDLKKGEALSLPRIKLSKMDVEWVHVLSEGWATPLQGFMRESEFLQTL 527
GKLVEL+V++ +R KK EA LPR++L+ +D++W+HVLSEGWA+PL GFMRESEFLQTL
Sbjct: 56 GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTL 115
Query: 528 HFNSLRLDDGTVVNMSVPIVLAIDDAQKHRIGDSKKVALFDSLGNPVAILKDIEIYKHPK 707
HFNSLRLDDG+VVNMSVPIVLAIDD QK RIG+S +VALF+S GNPVAIL DIEIYKHPK
Sbjct: 116 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPK 175
Query: 708 E 710
E
Sbjct: 176 E 176
>gb|AAM63185.1| ATP sulfurylase, putative [Arabidopsis thaliana]
Length = 463
Score = 214 bits (545), Expect = 9e-55
Identities = 116/181 (64%), Positives = 139/181 (76%)
Frame = +3
Query: 168 MASMAATTLFVKTPFPSHSLSKSFDTHFSPSLKVNVLTVAKARGVGSGLRVSNGLIEPDG 347
MASMAA + KTPF S L+KS P V+ + + R VGS + GLI PDG
Sbjct: 1 MASMAA--VLSKTPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGS---IRAGLIAPDG 55
Query: 348 GKLVELLVKDSERDLKKGEALSLPRIKLSKMDVEWVHVLSEGWATPLQGFMRESEFLQTL 527
GKLVEL+V++ +R KK EA LPR++L+ +D++W+HVLSEGW +PL GFMRESEFLQTL
Sbjct: 56 GKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWTSPLGGFMRESEFLQTL 115
Query: 528 HFNSLRLDDGTVVNMSVPIVLAIDDAQKHRIGDSKKVALFDSLGNPVAILKDIEIYKHPK 707
HFNSLRLDDG+VVNMSVPIVLAIDD QK RIG+S +VALF+S GNPV+IL DIEIYKHPK
Sbjct: 116 HFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVSILSDIEIYKHPK 175
Query: 708 E 710
E
Sbjct: 176 E 176
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 684,917,189
Number of Sequences: 1393205
Number of extensions: 16954947
Number of successful extensions: 81532
Number of sequences better than 10.0: 188
Number of HSP's better than 10.0 without gapping: 67506
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77372
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32654539052
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
MPDL079d06_f |
AV780588 |
1 |
621 |
| 2 |
MPDL008g08_f |
AV776939 |
26 |
371 |
| 3 |
MPDL035a09_f |
AV778221 |
150 |
711 |
| 4 |
MPD065g09_f |
AV774357 |
167 |
688 |
| 5 |
MPDL023g03_f |
AV777677 |
219 |
556 |
| 6 |
GNf011d06 |
BP068160 |
225 |
625 |
|
Lotus japonicus
Kazusa DNA Research Institute