>KMC002952A_C01 KMC002952A_c01
acatcaaatATTAAAAAAAAATATAAGTCTGAATACTATCAAATATTAAAAAAACATATC
CACATAAATTCACGACAGAAATACTGAATACATATGCCGTACCAACATGGCATGACAAAT
GAAGTCTAAGCTACGATAACTAGCTAGAAGTCCGCCATGATTAAAAGTATGTTTGGCTTG
AAATTTCAAAGCCTCTAAATGTATGTTTTCACCAATGGAACTAAATCTTTGCTTTCACAT
TGGAATGCTTTACTAAGGTGAGGACTAACCACATCCCTACGATATCAAACCCGAACTCTC
TTACTGCTGCATGAGGGGCACTTATAATGCTTAATGTGTTCTGGCCCGGGCAGGAGTGAT
CTTCACACACTTGCCATGGAACCAGTTCTCACATATGTCACAACAAATCCAGAACTCATC
AGATGCATAGTTCTCCCCACAAGCGCCACACAATGTCTCCCCATGCTCCTCATCATCTTC
TTCTTCATCCTCGTCCTTTACTTGCTTCAAATACTTCACTGATTCAGAGCCTCCCGCTTT
TGAACTGGTCTTTGTATTGTTGCTGGCGATGTTTGAAACTGATG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002952A_C01 KMC002952A_c01
         (584 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_197993.1| nucleic acid binding protein - like; protein id...   118  1e-30
pir||T01840 hypothetical protein F9D12.13 - Arabidopsis thaliana...   112  9e-29
ref|NP_197551.1| zinc finger protein - like; protein id: At5g205...   108  2e-28
ref|NP_189865.1| nucleic acid binding protein-like; protein id: ...   109  6e-28
ref|NP_178351.1| putative PHD-type zinc finger protein; protein ...    97  6e-25

>ref|NP_197993.1| nucleic acid binding protein - like; protein id: At5g26210.1,
           supported by cDNA: 32443. [Arabidopsis thaliana]
           gi|21592780|gb|AAM64729.1| nucleic acid binding
           protein-like [Arabidopsis thaliana]
          Length = 255

 Score =  118 bits (295), Expect(2) = 1e-30
 Identities = 55/88 (62%), Positives = 67/88 (75%), Gaps = 8/88 (9%)
 Frame = -3

Query: 582 SVSNIASNNTKTSSKAGGSESVKYLK-QVKDEDEEE-------DDEEHGETLCGACGENY 427
           SVSN +SN +K+SSK G     K+ K + KD++EEE       D++E GET CGACGE+Y
Sbjct: 150 SVSNNSSNRSKSSSKRGSESRAKFSKPEPKDDEEEEEEGVEEEDEDEQGETQCGACGESY 209

Query: 426 ASDEFWICCDICENWFHGKCVKITPARA 343
           A+DEFWICCD+CE WFHGKCVKITPARA
Sbjct: 210 AADEFWICCDLCEMWFHGKCVKITPARA 237

 Score = 36.6 bits (83), Expect(2) = 1e-30
 Identities = 14/17 (82%), Positives = 15/17 (87%)
 Frame = -1

Query: 341 EHIKHYKCPSCSSKRVR 291
           EHIK YKCPSCS+KR R
Sbjct: 238 EHIKQYKCPSCSNKRAR 254

>pir||T01840 hypothetical protein F9D12.13 - Arabidopsis thaliana
           gi|3319341|gb|AAC26230.1| similar to Medicago sativa
           nucleic acid binding protein Alfin-1 (GB:L07291)
           [Arabidopsis thaliana]
          Length = 251

 Score =  112 bits (279), Expect(2) = 9e-29
 Identities = 58/95 (61%), Positives = 70/95 (73%), Gaps = 15/95 (15%)
 Frame = -3

Query: 582 SVSNIASNNTKTSSKAG------GSES-VKYLK-QVKDEDEEE-------DDEEHGETLC 448
           SVSN +SN +K+SSK        GSES  K+ K + KD++EEE       D++E GET C
Sbjct: 139 SVSNNSSNRSKSSSKVETLFSTRGSESRAKFSKPEPKDDEEEEEEGVEEEDEDEQGETQC 198

Query: 447 GACGENYASDEFWICCDICENWFHGKCVKITPARA 343
           GACGE+YA+DEFWICCD+CE WFHGKCVKITPARA
Sbjct: 199 GACGESYAADEFWICCDLCEMWFHGKCVKITPARA 233

 Score = 36.6 bits (83), Expect(2) = 9e-29
 Identities = 14/17 (82%), Positives = 15/17 (87%)
 Frame = -1

Query: 341 EHIKHYKCPSCSSKRVR 291
           EHIK YKCPSCS+KR R
Sbjct: 234 EHIKQYKCPSCSNKRAR 250

>ref|NP_197551.1| zinc finger protein - like; protein id: At5g20510.1 [Arabidopsis
           thaliana]
          Length = 232

 Score =  108 bits (271), Expect(2) = 2e-28
 Identities = 42/51 (82%), Positives = 50/51 (97%)
 Frame = -3

Query: 495 DEDEEEDDEEHGETLCGACGENYASDEFWICCDICENWFHGKCVKITPARA 343
           +E+E+ED++EHGETLCGACG+NYASDEFWICCD+CE WFHG+CVKITPARA
Sbjct: 164 EEEEDEDEDEHGETLCGACGDNYASDEFWICCDMCEKWFHGECVKITPARA 214

 Score = 38.5 bits (88), Expect(2) = 2e-28
 Identities = 14/17 (82%), Positives = 16/17 (93%)
 Frame = -1

Query: 341 EHIKHYKCPSCSSKRVR 291
           EHIKHYKCP+CS+KR R
Sbjct: 215 EHIKHYKCPTCSNKRAR 231

>ref|NP_189865.1| nucleic acid binding protein-like; protein id: At3g42790.1,
           supported by cDNA: gi_17065549 [Arabidopsis thaliana]
           gi|11281497|pir||T47337 nucleic acid binding
           protein-like - Arabidopsis thaliana
           gi|7543887|emb|CAB87196.1| nucleic acid binding
           protein-like [Arabidopsis thaliana]
           gi|17065550|gb|AAL32929.1| nucleic acid binding
           protein-like [Arabidopsis thaliana]
           gi|21386979|gb|AAM47893.1| nucleic acid binding
           protein-like [Arabidopsis thaliana]
          Length = 250

 Score =  109 bits (272), Expect(2) = 6e-28
 Identities = 44/77 (57%), Positives = 59/77 (76%)
 Frame = -3

Query: 573 NIASNNTKTSSKAGGSESVKYLKQVKDEDEEEDDEEHGETLCGACGENYASDEFWICCDI 394
           N + +N+K  +  G S   K  K+  +E +E+D+++HGETLCGACG++  +DEFWICCD+
Sbjct: 156 NKSKSNSKVRTSEGKSSKTKQPKEEDEEIDEDDEDDHGETLCGACGDSDGADEFWICCDL 215

Query: 393 CENWFHGKCVKITPARA 343
           CE WFHGKCVKITPARA
Sbjct: 216 CEKWFHGKCVKITPARA 232

 Score = 36.6 bits (83), Expect(2) = 6e-28
 Identities = 14/17 (82%), Positives = 15/17 (87%)
 Frame = -1

Query: 341 EHIKHYKCPSCSSKRVR 291
           EHIK YKCPSCS+KR R
Sbjct: 233 EHIKQYKCPSCSNKRAR 249

>ref|NP_178351.1| putative PHD-type zinc finger protein; protein id: At2g02470.1,
           supported by cDNA: 38635. [Arabidopsis thaliana]
           gi|25346628|pir||A84437 probable PHD-type zinc finger
           protein [imported] - Arabidopsis thaliana
           gi|20197338|gb|AAM15031.1| putative PHD-type zinc finger
           protein [Arabidopsis thaliana]
           gi|28393037|gb|AAO41953.1| putative PHD-type zinc finger
           protein [Arabidopsis thaliana]
           gi|28827386|gb|AAO50537.1| putative PHD-type zinc finger
           protein [Arabidopsis thaliana]
          Length = 256

 Score = 97.4 bits (241), Expect(2) = 6e-25
 Identities = 42/80 (52%), Positives = 55/80 (68%), Gaps = 2/80 (2%)
 Frame = -3

Query: 576 SNIASNNTKTSSKAGGSESVKYLKQVKDEDEEEDDEEHGE--TLCGACGENYASDEFWIC 403
           S + S  +++ SK     S    ++ ++EDE ED+ E  E   +CGACG+NY +DEFWIC
Sbjct: 159 SGVKSRQSESLSKVAKMSSPPPKEEEEEEDESEDESEDDEQGAVCGACGDNYGTDEFWIC 218

Query: 402 CDICENWFHGKCVKITPARA 343
           CD CE WFHGKCVKITPA+A
Sbjct: 219 CDACEKWFHGKCVKITPAKA 238

 Score = 38.5 bits (88), Expect(2) = 6e-25
 Identities = 14/17 (82%), Positives = 16/17 (93%)
 Frame = -1

Query: 341 EHIKHYKCPSCSSKRVR 291
           EHIKHYKCP+CS+KR R
Sbjct: 239 EHIKHYKCPTCSNKRAR 255

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 491,035,274
Number of Sequences: 1393205
Number of extensions: 10643810
Number of successful extensions: 76569
Number of sequences better than 10.0: 251
Number of HSP's better than 10.0 without gapping: 45861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 65365
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21997688174
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf008d12 BP067956 1 492 2 SPD009a08_f BP044681 10 477 3 MFB040e07_f BP036927 10 409 4 MPDL069f11_f AV780042 10 522 5 SPD055h11_f BP048422 13 525 6 SPD095d01_f BP051584 16 177 7 MPD074e10_f AV774863 25 484 8 SPD018c03_f BP045402 25 563 9 SPD077a12_f BP050131 30 588 10 MR089g08_f BP082868 46 207

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