>KMC002929A_C01 KMC002929A_c01
CATGACCTTAGTCACAAAACAACAACTTTTTACTATTACAGCGAAGGCTCCCCTTCAAAC
AAATTTTACATAGACCAGACAAATTTATTAAATCCTAATGTCTGGATGATTCGTGGTCCT
CACAAGTCACTCCAAACTTAATTAGGGATGTTAAATACTAGGAATACAACAAGAAACTAT
CTAATACACCAGCACAACAATAACAATGCATAATAAAAAAGCACCATAGAACAATAGCTT
CATTTATCCTTACACCTGAACCAGAGAGGAGATGCAGGATTTTGCCTTGTGTTCCTGAGG
CAGATTTTCCCACCACTTTATGAAGGTAGGATCCTTTAAGAACACCTCCTTTTTAAGTTC
CCCTTTATCAGATTCAGTCCATTTCTTAAGGTTTTGTTCAAGTTGGAGAACCCTTTCCAT
CACATCTTCAAAAGGTTTCTTGTTACTAATCCAGCTGCATAGCTCTTCCATTTCAGCCCA
GAAGGTGGATTCTGAGATTTCTTCTTTTGGCACCCTCTTGGCATGCTCTAAC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002929A_C01 KMC002929A_c01
         (532 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM62411.1|AF480489_1 EDS1 [Nicotiana tabacum]                      76  3e-13
gb|AAL85347.1|AF479625_1 EDS1-like protein [Nicotiana benthamiana]     76  3e-13
pir||T48859 disease resistance protein EDS1 [validated] - Arabid...    62  3e-09
ref|NP_190392.1| disease resistance protein EDS1; protein id: At...    60  2e-08
ref|NP_190391.1| EDS1-like protein; protein id: At3g48080.1, sup...    58  8e-08

>gb|AAM62411.1|AF480489_1 EDS1 [Nicotiana tabacum]
          Length = 606

 Score = 75.9 bits (185), Expect = 3e-13
 Identities = 41/88 (46%), Positives = 52/88 (58%)
 Frame = -2

Query: 531 LEHAKRVPKEEISESTFWAEMEELCSWISNKKPFEDVMERVLQLEQNLKKWTESDKGELK 352
           LEH KRV   E SES FWAE+EEL       K   +V  R+L LE+  + W++S  G L 
Sbjct: 521 LEHDKRVQTGERSESCFWAEVEEL-----RNKSIMEVQNRILSLEKTARVWSQS--GLLG 573

Query: 351 KEVFLKDPTFIKWWENLPQEHKAKSCIS 268
            +VF  + TF KWW+ LP +HK  S IS
Sbjct: 574 DDVFFPESTFTKWWKQLPTQHKLASWIS 601

>gb|AAL85347.1|AF479625_1 EDS1-like protein [Nicotiana benthamiana]
          Length = 607

 Score = 75.9 bits (185), Expect = 3e-13
 Identities = 42/91 (46%), Positives = 51/91 (55%)
 Frame = -2

Query: 531 LEHAKRVPKEEISESTFWAEMEELCSWISNKKPFEDVMERVLQLEQNLKKWTESDKGELK 352
           LEH +RV   E SES FWAE+EEL       KP  +V  R+L LE   K W  S  G L 
Sbjct: 522 LEHEERVQTGERSESCFWAEVEEL-----RNKPIMEVQNRILSLET--KAWDWSQSGLLG 574

Query: 351 KEVFLKDPTFIKWWENLPQEHKAKSCISSLV 259
            +VF  + TF KWW+ LP +H+  S IS  V
Sbjct: 575 DDVFFPESTFTKWWKQLPTQHRMTSWISGKV 605

>pir||T48859 disease resistance protein EDS1 [validated] - Arabidopsis thaliana
           gi|4454567|gb|AAD20950.1| EDS1 [Arabidopsis thaliana]
          Length = 623

 Score = 62.4 bits (150), Expect = 3e-09
 Identities = 37/87 (42%), Positives = 51/87 (58%), Gaps = 2/87 (2%)
 Frame = -2

Query: 531 LEHAKRVPKEEISE--STFWAEMEELCSWISNKKPFEDVMERVLQLEQNLKKWTESDKGE 358
           LE  +   K   SE  S FWAE+EEL       KP+E+V  RV  LE  L++W  + + +
Sbjct: 530 LEEIQETLKNSGSECGSCFWAEVEEL-----KGKPYEEVEVRVKTLEGMLREWITAGEVD 584

Query: 357 LKKEVFLKDPTFIKWWENLPQEHKAKS 277
            +KE+FL+  TF KWW  LP+ HK+ S
Sbjct: 585 -EKEIFLEGSTFRKWWITLPKNHKSHS 610

>ref|NP_190392.1| disease resistance protein EDS1; protein id: At3g48090.1, supported
           by cDNA: gi_15028150 [Arabidopsis thaliana]
           gi|7487125|pir||T06674 hypothetical protein T17F15.40 -
           Arabidopsis thaliana gi|4678319|emb|CAB41130.1| putative
           protein [Arabidopsis thaliana]
           gi|15028151|gb|AAK76699.1| putative disease resistance
           protein EDS1 [Arabidopsis thaliana]
           gi|23297426|gb|AAN12884.1| putative disease resistance
           protein EDS1 [Arabidopsis thaliana]
          Length = 623

 Score = 59.7 bits (143), Expect = 2e-08
 Identities = 37/87 (42%), Positives = 48/87 (54%), Gaps = 2/87 (2%)
 Frame = -2

Query: 531 LEHAKRVPKEEISE--STFWAEMEELCSWISNKKPFEDVMERVLQLEQNLKKWTESDKGE 358
           LE  +   K   SE  S FWAE+EEL       KP+E+V  RV  LE  L +W    + +
Sbjct: 530 LEEIQETLKNSGSECGSCFWAEVEEL-----KGKPYEEVEVRVKTLEGMLGEWITDGEVD 584

Query: 357 LKKEVFLKDPTFIKWWENLPQEHKAKS 277
             KE+FL+  TF KWW  LP+ HK+ S
Sbjct: 585 -DKEIFLEGSTFRKWWITLPKNHKSHS 610

>ref|NP_190391.1| EDS1-like protein; protein id: At3g48080.1, supported by cDNA:
           gi_18087549 [Arabidopsis thaliana]
           gi|7487126|pir||T06675 hypothetical protein T17F15.50 -
           Arabidopsis thaliana gi|4678320|emb|CAB41131.1|
           hypothetical protein [Arabidopsis thaliana]
           gi|18087550|gb|AAL58907.1|AF462816_1 AT3g48080/T17F15_50
           [Arabidopsis thaliana] gi|23463067|gb|AAN33203.1|
           At3g48080/T17F15_50 [Arabidopsis thaliana]
          Length = 629

 Score = 57.8 bits (138), Expect = 8e-08
 Identities = 30/68 (44%), Positives = 40/68 (58%)
 Frame = -2

Query: 489 STFWAEMEELCSWISNKKPFEDVMERVLQLEQNLKKWTESDKGELKKEVFLKDPTFIKWW 310
           S FWAE+EEL       KP+E+V  R   LE  L+ W +  + + +KE+FL+  TF KWW
Sbjct: 550 SCFWAEVEEL-----KGKPYEEVQVRFKTLEGLLEGWIKDGEVD-EKEIFLEGSTFRKWW 603

Query: 309 ENLPQEHK 286
             LP  HK
Sbjct: 604 NTLPDSHK 611

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 470,293,491
Number of Sequences: 1393205
Number of extensions: 10507676
Number of successful extensions: 24800
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 23843
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24782
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17596710992
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR076f12_f BP081871 1 366 2 GNf091d10 BP074086 80 466 3 GNf006f03 BP067821 85 506 4 MRL042d11_f BP085767 90 461 5 MWM146g06_f AV767011 93 612 6 MWL021h09_f AV768933 107 306 7 MF095b04_f BP033243 108 562

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