Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002911A_C02 KMC002911A_c02
(912 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_565647.1| similar to PIG-L; protein id: At2g27340.1 [Arab... 117 2e-25
pir||G84671 hypothetical protein At2g27340 [imported] - Arabidop... 68 2e-11
ref|NP_004269.1| phosphatidylinositol glycan, class L; N-acetylg... 69 1e-10
ref|NP_620256.1| phosphatidylinositol glycan, class L [Rattus no... 68 2e-10
ref|XP_111044.1| similar to N-acetylglucosaminyl-phosphatidylino... 67 3e-10
>ref|NP_565647.1| similar to PIG-L; protein id: At2g27340.1 [Arabidopsis thaliana]
gi|20197648|gb|AAM15175.1| similar to PIG-L [Arabidopsis
thaliana] gi|20197670|gb|AAD41996.2| similar to PIG-L
[Arabidopsis thaliana]
Length = 223
Score = 117 bits (293), Expect = 2e-25
Identities = 64/120 (53%), Positives = 77/120 (63%), Gaps = 2/120 (1%)
Frame = -2
Query: 893 HCLDM-IITFDNYGVSGPCNHRDVHYGV-CKLLHDTLRKDVEVWELISTNILRKYSGPVD 720
HC + IITFDNYGV G CNHRDVH + CK+ +S NI RKY GPVD
Sbjct: 98 HCNEEHIITFDNYGVWGHCNHRDVHPPIDCKIDSAKRIHGFLYVHQVSLNIFRKYCGPVD 157
Query: 719 IWLSMFWAMLHSNGTMQCLVNEHYCRSLKAMAQHSRQWVWFRKLFVALSSYTYMNTLRKI 540
IWLS+ A H + + ++N+ +S KAMAQH QWVWFRKLFV SSYTY+NTL +I
Sbjct: 158 IWLSILSAKRHPSKVI--IINKQPWKSFKAMAQHLSQWVWFRKLFVLFSSYTYVNTLDRI 215
>pir||G84671 hypothetical protein At2g27340 [imported] - Arabidopsis thaliana
Length = 185
Score = 67.8 bits (164), Expect(2) = 2e-11
Identities = 40/84 (47%), Positives = 46/84 (54%), Gaps = 1/84 (1%)
Frame = -2
Query: 893 HCLDM-IITFDNYGVSGPCNHRDVHYGVCKLLHDTLRKDVEVWELISTNILRKYSGPVDI 717
HC + IITFDNYGV G CNHRDVH GV +S NI RKY GPVDI
Sbjct: 111 HCNEEHIITFDNYGVWGHCNHRDVHRGVL---------------YVSLNIFRKYCGPVDI 155
Query: 716 WLSMFWAMLHSNGTMQCLVNEHYC 645
WLS+ A H + V+E +C
Sbjct: 156 WLSILSAKRHPS-----KVSEAFC 174
Score = 23.9 bits (50), Expect(2) = 2e-11
Identities = 9/17 (52%), Positives = 14/17 (81%)
Frame = -1
Query: 600 VSQAFCCVVELYLYEHS 550
VS+AFC V +LY+ E++
Sbjct: 169 VSEAFCFVFKLYICEYT 185
>ref|NP_004269.1| phosphatidylinositol glycan, class L;
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
[Homo sapiens] gi|14916637|sp|Q9Y2B2|PIGL_HUMAN
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
(Phosphatidylinositol-glycan biosynthesis, class L
protein) (PIG-L) gi|4239986|dbj|BAA74775.1| PIG-L [Homo
sapiens]
Length = 252
Score = 68.6 bits (166), Expect = 1e-10
Identities = 42/122 (34%), Positives = 68/122 (55%), Gaps = 2/122 (1%)
Frame = -2
Query: 908 EEITSHCLDMIITFDNYGVSGPCNHRDVHYGVCKLLHDT--LRKDVEVWELISTNILRKY 735
+ I + +++++TFD GVSG NH + Y + LH L K V L S N+LRKY
Sbjct: 133 QHIEVNGINLVVTFDAGGVSGHSNHIAL-YAAVRALHSEGKLPKGCSVLTLQSVNVLRKY 191
Query: 734 SGPVDIWLSMFWAMLHSNGTMQCLVNEHYCRSLKAMAQHSRQWVWFRKLFVALSSYTYMN 555
+D+ LS+ LH+ + L ++ ++ KAM+ H Q +WFR+L++ S Y +N
Sbjct: 192 ISLLDLPLSL----LHTQDVLFVLNSKEVAQAKKAMSCHRSQLLWFRRLYIIFSRYMRIN 247
Query: 554 TL 549
+L
Sbjct: 248 SL 249
>ref|NP_620256.1| phosphatidylinositol glycan, class L [Rattus norvegicus]
gi|14916624|sp|O35790|PIGL_RAT
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
(Phosphatidylinositol-glycan biosynthesis, class L
protein) (PIG-L) gi|2225906|dbj|BAA20869.1| PIG-L
[Rattus norvegicus]
Length = 252
Score = 67.8 bits (164), Expect = 2e-10
Identities = 41/123 (33%), Positives = 65/123 (52%), Gaps = 2/123 (1%)
Frame = -2
Query: 908 EEITSHCLDMIITFDNYGVSGPCNHRDVHYGVCKLLHD--TLRKDVEVWELISTNILRKY 735
+ I ++ D+++TFD GVSG NH ++ V + LH L + V L S N+LRKY
Sbjct: 133 QHIHANATDLVVTFDAEGVSGHSNHIALYKAV-RALHSGGKLPEGCSVLTLQSVNVLRKY 191
Query: 734 SGPVDIWLSMFWAMLHSNGTMQCLVNEHYCRSLKAMAQHSRQWVWFRKLFVALSSYTYMN 555
L + W +L G + L ++ ++ KAM+ H Q +WFR L+ S Y +N
Sbjct: 192 V----FLLDLPWTLLSPQGVLFVLTSKEVAQAKKAMSCHRSQLLWFRHLYTVFSRYMSVN 247
Query: 554 TLR 546
+L+
Sbjct: 248 SLQ 250
>ref|XP_111044.1| similar to N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
(Phosphatidylinositol-glycan biosynthesis, class L
protein) (PIG-L) [Mus musculus]
Length = 188
Score = 67.4 bits (163), Expect = 3e-10
Identities = 43/123 (34%), Positives = 65/123 (51%), Gaps = 2/123 (1%)
Frame = -2
Query: 908 EEITSHCLDMIITFDNYGVSGPCNHRDVHYGVCKLLHD--TLRKDVEVWELISTNILRKY 735
+ I ++ D+++TFD GVSG NH ++ V + LH L K V L S N LRKY
Sbjct: 69 QHIHANGTDLVVTFDAEGVSGHSNHIALYKAV-RALHSGGKLPKGCSVLTLQSVNALRKY 127
Query: 734 SGPVDIWLSMFWAMLHSNGTMQCLVNEHYCRSLKAMAQHSRQWVWFRKLFVALSSYTYMN 555
+ L + W +L + L ++ ++ KAM+ H Q +WFR L+V S Y +N
Sbjct: 128 A----FLLDLPWTLLSPQDVLFVLTSKEVAQAKKAMSCHRSQLLWFRYLYVLFSRYMRIN 183
Query: 554 TLR 546
+LR
Sbjct: 184 SLR 186
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 721,086,537
Number of Sequences: 1393205
Number of extensions: 15231299
Number of successful extensions: 32545
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 31392
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32505
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 49918505760
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
