KMC002885A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002885A_C01 KMC002885A_c01
atAACAGATCAAAAGATTAATTTTCATTGATGATTAAAGGCATATGATAACTACATCAAT
CTAATTTTACAAAATCAGAAGCAATAAAGAACATTAAAATACAATTAAAAGAAAGGAGTC
AAACACCTAAGGTAGTCCCTAAAAACACCATACTAGTCCTTTTTGAGGGACTAAACTAAT
ATGACTGAAGAAAAGATGAAATTTGTACATTTTGCTTTGGTGTTTGAAACACAAACTAGC
ATTTTGGACAAAAGCTTGAGCTACAAGAAACAAACTTTTAACCAAACCATGATTGGAATT
CACCAAACACCAAATTAAAAGTGCACTAACTTGTCCAAAACCAAATTTTTCAAAGACAAA
TTTATTCACTCCAATACTTCTCATCACCCTCTTTACCCTCTCCTTCTTCATCCTCAACCA
AACTAGCCTTAAAACTCTCAACATCTTCCACCGCGGCGCTCCTAAACAAACCCACATCCA
CAGCACCAGACACCATGTGATACCCCCGTGACCTCAAGTCTCGCGGCCCGTCGTATGGCA
ACGCGAAA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002885A_C01 KMC002885A_c01
         (548 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_192813.1| putative aldolase; protein id: At4g10750.1, sup...    81  9e-15
gb|AAB81520.1| putative aldolase [Arabidopsis thaliana]                80  2e-14
gb|AAM64963.1| putative aldolase [Arabidopsis thaliana]                78  6e-14
ref|NP_194138.1| putative protein; protein id: At4g24070.1 [Arab...    49  4e-05
gb|AAB63616.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase i...    44  0.001

>ref|NP_192813.1| putative aldolase; protein id: At4g10750.1, supported by cDNA:
           35159. [Arabidopsis thaliana] gi|7486898|pir||T01902
           hypothetical protein T12H20.10 - Arabidopsis thaliana
           gi|3600031|gb|AAC35519.1| similar to
           2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolases
           [Arabidopsis thaliana] gi|7267773|emb|CAB81176.1|
           putative aldolase [Arabidopsis thaliana]
           gi|26451478|dbj|BAC42838.1| putative aldolase
           [Arabidopsis thaliana]
          Length = 358

 Score = 80.9 bits (198), Expect = 9e-15
 Identities = 39/67 (58%), Positives = 47/67 (69%), Gaps = 6/67 (8%)
 Frame = -2

Query: 547 FALPYDGPRDLRSRGYHMVSGAVDVGLFRSAAVEDVESFKASLVEDEEGEGKE------G 386
           FA+P+DG  ++R RGYHMV+GAVDVGLFR+AAVEDV  FK  LV + + E          
Sbjct: 292 FAMPHDGAGEIRGRGYHMVAGAVDVGLFRNAAVEDVRRFKMGLVNESDSEDSSEHDKDVD 351

Query: 385 DEKYWSE 365
           DEKYWSE
Sbjct: 352 DEKYWSE 358

>gb|AAB81520.1| putative aldolase [Arabidopsis thaliana]
          Length = 248

 Score = 79.7 bits (195), Expect = 2e-14
 Identities = 38/67 (56%), Positives = 47/67 (69%), Gaps = 6/67 (8%)
 Frame = -2

Query: 547 FALPYDGPRDLRSRGYHMVSGAVDVGLFRSAAVEDVESFKASLVEDEEGEGKE------G 386
           FA+P+DG  ++R RGYHMV+GAVDVGLFR+AAVEDV  FK  LV + + E          
Sbjct: 175 FAMPHDGAGEIRGRGYHMVAGAVDVGLFRNAAVEDVRRFKMGLVNESDSEDSSEHDKDVD 234

Query: 385 DEKYWSE 365
           DEKYWS+
Sbjct: 235 DEKYWSD 241

>gb|AAM64963.1| putative aldolase [Arabidopsis thaliana]
          Length = 358

 Score = 78.2 bits (191), Expect = 6e-14
 Identities = 38/67 (56%), Positives = 46/67 (67%), Gaps = 6/67 (8%)
 Frame = -2

Query: 547 FALPYDGPRDLRSRGYHMVSGAVDVGLFRSAAVEDVESFKASLVEDEEGEGKE------G 386
           FA+P+DG  ++R  GYHMV+GAVDVGLFR+AAVEDV  FK  LV + + E          
Sbjct: 292 FAMPHDGAGEIRGPGYHMVAGAVDVGLFRNAAVEDVRRFKMGLVNESDSEDSSEHDKDVD 351

Query: 385 DEKYWSE 365
           DEKYWSE
Sbjct: 352 DEKYWSE 358

>ref|NP_194138.1| putative protein; protein id: At4g24070.1 [Arabidopsis thaliana]
           gi|7487176|pir||T13453 hypothetical protein T19F6.60 -
           Arabidopsis thaliana gi|2262109|gb|AAB63617.1|
           hypothetical protein [Arabidopsis thaliana]
           gi|5668635|emb|CAB51650.1| putative protein [Arabidopsis
           thaliana] gi|7269256|emb|CAB81325.1| putative protein
           [Arabidopsis thaliana]
          Length = 66

 Score = 48.9 bits (115), Expect = 4e-05
 Identities = 23/39 (58%), Positives = 28/39 (70%)
 Frame = -2

Query: 544 ALPYDGPRDLRSRGYHMVSGAVDVGLFRSAAVEDVESFK 428
           A   D   DL+SRGYHM+ GA DV LF+ A V+DV+SFK
Sbjct: 24  ATAQDKAVDLKSRGYHMILGATDVPLFKKAVVDDVKSFK 62

>gb|AAB63616.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase isolog [Arabidopsis
           thaliana]
          Length = 250

 Score = 43.9 bits (102), Expect = 0.001
 Identities = 19/40 (47%), Positives = 28/40 (69%)
 Frame = -2

Query: 544 ALPYDGPRDLRSRGYHMVSGAVDVGLFRSAAVEDVESFKA 425
           A   D   DL++RGYH+V G+ DV L++ A V++V +FKA
Sbjct: 211 ATAQDKTGDLKARGYHVVLGSTDVSLYKKAVVDEVNAFKA 250

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 440,382,658
Number of Sequences: 1393205
Number of extensions: 9262253
Number of successful extensions: 32705
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 30130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32504
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18947112822
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MR020h05_f BP077554 1 411
2 MF086f11_f BP032835 3 466
3 MR055a06_f BP080210 4 557
4 MF006g04_f BP028561 8 474
5 GNf003e03 BP067595 46 451




Lotus japonicus
Kazusa DNA Research Institute