Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002866A_C01 KMC002866A_c01
(632 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_200674.1| putative protein; protein id: At5g58660.1 [Arab... 131 1e-29
ref|NP_190303.1| 1-aminocyclopropane-1-carboxylic acid oxidase -... 117 2e-25
ref|NP_193852.1| gibberellin 20-oxidase - like protein; protein ... 109 3e-23
dbj|BAC10398.1| gibberellin 20-oxidase-like protein [Oryza sativ... 100 2e-20
ref|NP_175509.1| gibberellin 20-oxidase, putative; protein id: A... 95 9e-19
>ref|NP_200674.1| putative protein; protein id: At5g58660.1 [Arabidopsis thaliana]
gi|8843787|dbj|BAA97335.1| gibberellin oxidase-like
protein [Arabidopsis thaliana]
Length = 352
Score = 131 bits (329), Expect(2) = 1e-29
Identities = 62/107 (57%), Positives = 85/107 (78%), Gaps = 1/107 (0%)
Frame = -1
Query: 632 HTDSSILSIXNQDDQVSGLEVLKDDRWLTVKPISDTLIVNLGDMMQAISNDRYKSVSHRV 453
HTDSS++SI +D+ GLE++K + W VKP+++TLIVNLGDMMQAIS+D YKSV+HRV
Sbjct: 229 HTDSSVISILREDES-GGLEIMKGEEWFCVKPVANTLIVNLGDMMQAISDDEYKSVTHRV 287
Query: 452 KV-SKNIDRISICYFVFPGEDVVIESTKYKPFTYNEFRAQVQQDIKA 315
K ++ +R S+CYFVFP D VI+S+ YK FTY++F AQVQ D+++
Sbjct: 288 KKRNRKTERHSVCYFVFPKRDCVIKSSNYKLFTYSDFEAQVQADVQS 334
Score = 20.8 bits (42), Expect(2) = 1e-29
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -2
Query: 313 LGYKVGLARFN 281
LG K+GL RFN
Sbjct: 335 LGTKIGLPRFN 345
>ref|NP_190303.1| 1-aminocyclopropane-1-carboxylic acid oxidase - like protein;
protein id: At3g47190.1 [Arabidopsis thaliana]
gi|11255896|pir||T45655 1-aminocyclopropane-1-carboxylic
acid oxidase-like protein - Arabidopsis thaliana
gi|6522600|emb|CAB61965.1|
1-aminocyclopropane-1-carboxylic acid oxidase-like
protein [Arabidopsis thaliana]
Length = 345
Score = 117 bits (292), Expect = 2e-25
Identities = 57/106 (53%), Positives = 77/106 (71%), Gaps = 1/106 (0%)
Frame = -1
Query: 632 HTDSSILSIXNQDDQVSGLEVLKDDRWLTVKPISDTLIVNLGDMMQAISNDRYKSVSHRV 453
HTDSS++SI NQDD V GLE +KD W VKP++ + +V LGDMMQ IS++ YKSV H+V
Sbjct: 226 HTDSSVISIINQDD-VGGLEFMKDGEWFNVKPLASSFVVGLGDMMQVISDEEYKSVLHKV 284
Query: 452 -KVSKNIDRISICYFVFPGEDVVIESTKYKPFTYNEFRAQVQQDIK 318
K + +R SI FVFP +D + ST+YKPF ++EF AQV+ D++
Sbjct: 285 GKRMRKKERYSIVNFVFPDKDCMFNSTRYKPFKFSEFEAQVKLDVE 330
>ref|NP_193852.1| gibberellin 20-oxidase - like protein; protein id: At4g21200.1
[Arabidopsis thaliana] gi|7433237|pir||T04951
hypothetical protein F7J7.140 - Arabidopsis thaliana
gi|2911077|emb|CAA17539.1| gibberellin 20-oxidase-like
protein [Arabidopsis thaliana]
gi|7268917|emb|CAB79120.1| gibberellin 20-oxidase-like
protein [Arabidopsis thaliana]
Length = 293
Score = 109 bits (272), Expect = 3e-23
Identities = 54/109 (49%), Positives = 73/109 (66%), Gaps = 4/109 (3%)
Frame = -1
Query: 632 HTDSSILSIXNQDDQVSGLEVLKDDRWLTVKPISDTLIVNLGDMMQAISNDRYKSVSHRV 453
HTDS L+I QD QV GL+++KD+RW+ VKP LI+N+GD+ QA SN YKSV HRV
Sbjct: 170 HTDSDFLTILYQD-QVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRV 228
Query: 452 KVSKNIDRISICYFVFPGEDVVIESTK----YKPFTYNEFRAQVQQDIK 318
+ ++R S YF+ P D VIE + Y+ F++ EFR QVQ+D+K
Sbjct: 229 MTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVK 277
>dbj|BAC10398.1| gibberellin 20-oxidase-like protein [Oryza sativa (japonica
cultivar-group)]
Length = 341
Score = 100 bits (249), Expect = 2e-20
Identities = 49/116 (42%), Positives = 74/116 (63%), Gaps = 10/116 (8%)
Frame = -1
Query: 632 HTDSSILSIXNQD-DQVSGLEVLKDDRWLTVKPISDTLIVNLGDMMQAISNDRYKSVSHR 456
HTDS +L+I +Q D V GL++LK RW+ VKP TLIVN+GD++QA SND YKSV HR
Sbjct: 213 HTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEHR 272
Query: 455 VKVSKNIDRISICYFVFPGEDVVI---------ESTKYKPFTYNEFRAQVQQDIKA 315
V + ++R S+ +F+ P +I + Y+ FT+ E+R Q+ +D+++
Sbjct: 273 VMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRS 328
>ref|NP_175509.1| gibberellin 20-oxidase, putative; protein id: At1g50960.1
[Arabidopsis thaliana] gi|25285687|pir||G96546 probable
gibberellin 20-oxidase [imported] - Arabidopsis thaliana
gi|12321803|gb|AAG50945.1|AC079284_20 gibberellin
20-oxidase, putative [Arabidopsis thaliana]
Length = 336
Score = 94.7 bits (234), Expect = 9e-19
Identities = 48/109 (44%), Positives = 73/109 (66%), Gaps = 4/109 (3%)
Frame = -1
Query: 632 HTDSSILSIXNQDDQVSGLEVLKDDRWLTVKPISDTLIVNLGDMMQAISNDRYKSVSHRV 453
HTD+S L+I +QD Q+ GLE+ + +W++VKP + L VN+GDM QA+SN Y+SV HRV
Sbjct: 216 HTDTSFLTILSQD-QIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRV 274
Query: 452 KVSKNIDRISICYFVFPGEDVVIE----STKYKPFTYNEFRAQVQQDIK 318
NI+R+SI +FV P + I+ KY+ F++ E++ Q + D+K
Sbjct: 275 ISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVK 323
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 501,892,088
Number of Sequences: 1393205
Number of extensions: 10423198
Number of successful extensions: 28469
Number of sequences better than 10.0: 648
Number of HSP's better than 10.0 without gapping: 27123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28216
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26154777244
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
