Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002864A_C02 KMC002864A_c02
(715 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_192979.1| pectinesterase (pectin methylesterase) family; ... 199 3e-50
gb|AAM94923.1| putative pollen specific protein [Oryza sativa (j... 179 3e-44
dbj|BAA90610.1| ESTs AU082304(C61278),C93463(C50982) correspond ... 173 2e-42
ref|NP_194254.2| pectinesterase (pectin methylesterase) family; ... 172 4e-42
ref|NP_199961.1| pectinesterase (pectin methylesterase) family; ... 170 2e-41
>ref|NP_192979.1| pectinesterase (pectin methylesterase) family; protein id:
At4g12420.1, supported by cDNA: gi_18158153 [Arabidopsis
thaliana] gi|7433001|pir||T07634 pollen-specific protein
homolog T1P17.10 - Arabidopsis thaliana
gi|4725941|emb|CAB41712.1| putative pollen-specific
protein [Arabidopsis thaliana]
gi|7267944|emb|CAB78285.1| putative pollen-specific
protein [Arabidopsis thaliana]
gi|18158154|gb|AAL62306.1|AF439406_1 multi-copper
oxidase-related protein [Arabidopsis thaliana]
Length = 587
Score = 199 bits (506), Expect = 3e-50
Identities = 90/117 (76%), Positives = 101/117 (85%)
Frame = -1
Query: 715 HLDGYAFFVVGMDYGDWSDNSRGTYNKWDGIARATTQVYPGAWTAILVSLDNVGIWNLRT 536
H+ GYAFFVVGMDYG+W++NSRGTYNKWDGIAR+T QVYPGAW+AIL+SLDN G WNLRT
Sbjct: 452 HMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRT 511
Query: 535 ENLDSWYLGQETYIRVVNPEPNNKTELPIPDHALFCGALSKKPKPQDVSSSGSSING 365
ENLDSWYLGQETY+RVVNP+ NNKTE PD+ L+CGALSK KPQ VSSS S G
Sbjct: 512 ENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALSKLQKPQKVSSSASKSIG 568
>gb|AAM94923.1| putative pollen specific protein [Oryza sativa (japonica
cultivar-group)] gi|22324950|gb|AAM95677.1| putative
ascorbate oxidase [Oryza sativa (japonica
cultivar-group)]
Length = 573
Score = 179 bits (455), Expect = 3e-44
Identities = 79/106 (74%), Positives = 94/106 (88%), Gaps = 1/106 (0%)
Frame = -1
Query: 715 HLDGYAFFVVGMDYGDWSDNSRGTYNKWDGIARATTQVYPGAWTAILVSLDNVGIWNLRT 536
H+DGYAFFVVGMDYG+W+++SRGTYNK DG+AR+T QVYPGAW A+LVSLDNVG+WN+R+
Sbjct: 434 HMDGYAFFVVGMDYGEWTEDSRGTYNKGDGVARSTVQVYPGAWAAVLVSLDNVGVWNVRS 493
Query: 535 ENLDSWYLGQETYIRVVNPE-PNNKTELPIPDHALFCGALSKKPKP 401
ENLDSWYLGQE Y+RVVNPE NKTE+ IPD+ALFCG L K+ P
Sbjct: 494 ENLDSWYLGQEVYVRVVNPEDTGNKTEMAIPDNALFCGQLHKQQTP 539
>dbj|BAA90610.1| ESTs AU082304(C61278),C93463(C50982) correspond to a region of the
predicted gene.~Similar to Arabidopsis thaliana DNA
chromosome 4, BAC clone F24A6, Pollen-specific protein
precursor like. (AL035396) [Oryza sativa (japonica
cultivar-group)]
Length = 593
Score = 173 bits (438), Expect = 2e-42
Identities = 76/115 (66%), Positives = 95/115 (82%), Gaps = 1/115 (0%)
Frame = -1
Query: 715 HLDGYAFFVVGMDYGDWSDNSRGTYNKWDGIARATTQVYPGAWTAILVSLDNVGIWNLRT 536
H+DGYAF+VVGMDYG+W++NSRGTYNKWDG++R TTQV+PGAWTA+++SLD+ G WN+RT
Sbjct: 454 HIDGYAFWVVGMDYGEWTENSRGTYNKWDGVSRCTTQVFPGAWTAVMLSLDSPGFWNVRT 513
Query: 535 ENLDSWYLGQETYIRVVNPEPN-NKTELPIPDHALFCGALSKKPKPQDVSSSGSS 374
ENLD+WYLGQETYIRVV+P+ N TE+ PD+ L+CG L K K Q S SS
Sbjct: 514 ENLDTWYLGQETYIRVVDPDGGYNVTEMVAPDNMLYCGLLKDKQKAQKPHGSSSS 568
>ref|NP_194254.2| pectinesterase (pectin methylesterase) family; protein id:
At4g25240.1, supported by cDNA: gi_18389263, supported
by cDNA: gi_20259624 [Arabidopsis thaliana]
gi|18389264|gb|AAL67075.1| putative Pollen-specific
protein precursor [Arabidopsis thaliana]
gi|20259625|gb|AAM14169.1| putative pollen-specific
protein precursor [Arabidopsis thaliana]
Length = 589
Score = 172 bits (436), Expect = 4e-42
Identities = 74/128 (57%), Positives = 100/128 (77%)
Frame = -1
Query: 715 HLDGYAFFVVGMDYGDWSDNSRGTYNKWDGIARATTQVYPGAWTAILVSLDNVGIWNLRT 536
H+DGY+FFVVGMD+G WS++ +G+YN WD I+R+T +VYPG WTA+L+SLDNVG+WN+R
Sbjct: 455 HVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRV 514
Query: 535 ENLDSWYLGQETYIRVVNPEPNNKTELPIPDHALFCGALSKKPKPQDVSSSGSSINGNIL 356
ENLD WYLG+ETY+R+ NPE + KTE+ PD+ L+CGAL K Q S++ S +NG+ L
Sbjct: 515 ENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYCGALKNLQKEQHHSAATSILNGH-L 573
Query: 355 KLFFTWVM 332
KL V+
Sbjct: 574 KLMLLMVL 581
>ref|NP_199961.1| pectinesterase (pectin methylesterase) family; protein id:
At5g51480.1 [Arabidopsis thaliana]
gi|9758190|dbj|BAB08664.1| pectinesterase-like; strong
similarity to pollen-specific protein [Arabidopsis
thaliana] gi|28393459|gb|AAO42151.1| unknown protein
[Arabidopsis thaliana] gi|28827358|gb|AAO50523.1|
unknown protein [Arabidopsis thaliana]
Length = 592
Score = 170 bits (431), Expect = 2e-41
Identities = 71/135 (52%), Positives = 100/135 (73%)
Frame = -1
Query: 715 HLDGYAFFVVGMDYGDWSDNSRGTYNKWDGIARATTQVYPGAWTAILVSLDNVGIWNLRT 536
H+DGYAF+VV MD+G WS++ +YN WD +AR+T +VYPGAWTA+L+SLDNVG+WN+R
Sbjct: 455 HIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRV 514
Query: 535 ENLDSWYLGQETYIRVVNPEPNNKTELPIPDHALFCGALSKKPKPQDVSSSGSSINGNIL 356
ENLD WYLGQETY+R++NPE N TE+ P++ ++CGAL K Q SS+ S+ L
Sbjct: 515 ENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAMQKEQHHSSATKSMTNGQL 574
Query: 355 KLFFTWVMFVCTTIT 311
L F+ +M + ++ +
Sbjct: 575 ILIFSMMMVLLSSFS 589
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 688,846,188
Number of Sequences: 1393205
Number of extensions: 16323001
Number of successful extensions: 55120
Number of sequences better than 10.0: 172
Number of HSP's better than 10.0 without gapping: 48908
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 54234
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32936043699
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
