Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002834A_C04 KMC002834A_c04
(1361 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC10996.1| flavanone 3-hydroxylase [Nierembergia sp. NB17] 635 0.0
gb|AAC49929.1| flavanone 3beta-hydroxylase [Petunia x hybrida] 633 e-180
pir||A42110 flavanone 3 beta-hydroxylase - garden petunia (fragm... 633 e-180
sp|Q07353|FL3H_PETHY Naringenin,2-oxoglutarate 3-dioxygenase (Fl... 632 e-180
gb|AAD56577.1|AF184270_1 flavanone 3-hydroxylase [Daucus carota] 629 e-179
>dbj|BAC10996.1| flavanone 3-hydroxylase [Nierembergia sp. NB17]
Length = 367
Score = 635 bits (1638), Expect = 0.0
Identities = 310/365 (84%), Positives = 339/365 (91%), Gaps = 4/365 (1%)
Frame = -3
Query: 1311 PKTLTTLAQQNTLESSFVRDEDERPKVAYNNFSNEIPVISLAGIDE-VDGRRSEICNKIV 1135
P TLT LA++ TL++SF+RDEDERPKVAYN FSNEIP+ISL GID+ G+R EIC+KIV
Sbjct: 3 PSTLTALAEEKTLQTSFIRDEDERPKVAYNQFSNEIPIISLKGIDDDQSGKRGEICDKIV 62
Query: 1134 EACENWGIFQVVDHGVDTELVSHMTTLAKEFFALPPEEKLRFDMTGGKKGGFIVSSHLQG 955
+ACE+WGIFQVVDHGVD EL++ MTTLAKEFFALPPEEKLRFDM+GGKKGGFIVSSHLQG
Sbjct: 63 KACEDWGIFQVVDHGVDVELINKMTTLAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQG 122
Query: 954 ESVQDWREIVTYFSYPIRNRDYSRWPDTPAGWKAVTEEYSEKLMGLACKLLEVLSEAMGL 775
E+VQDWREIVTYFSYPIR RDYSRWP+ P GW AVTE+YS+ LM LACKLLEVLSEAMGL
Sbjct: 123 EAVQDWREIVTYFSYPIRARDYSRWPNKPEGWIAVTEKYSDTLMALACKLLEVLSEAMGL 182
Query: 774 EKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNG 595
EKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+DNG
Sbjct: 183 EKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNG 242
Query: 594 KTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVY 415
KTWITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSNSSRLSIATFQNPAP+A VY
Sbjct: 243 KTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVY 302
Query: 414 PLKVREGEKSVMEEPITFAEMYRRKMSKDIELARMKKLAKEKKLQ---DLEKAKLEPKPM 244
PLK+REGEKS+MEEPITFAEMYR+KMSKD+ELAR+KKLAKE++LQ EK KLE KP+
Sbjct: 303 PLKIREGEKSIMEEPITFAEMYRKKMSKDLELARLKKLAKEQQLQAEVAAEKTKLESKPI 362
Query: 243 NEIFA 229
EIFA
Sbjct: 363 EEIFA 367
>gb|AAC49929.1| flavanone 3beta-hydroxylase [Petunia x hybrida]
Length = 366
Score = 633 bits (1632), Expect = e-180
Identities = 306/364 (84%), Positives = 337/364 (92%), Gaps = 3/364 (0%)
Frame = -3
Query: 1311 PKTLTTLAQQNTLESSFVRDEDERPKVAYNNFSNEIPVISLAGIDEVDGRRSEICNKIVE 1132
P TLT LA++ TL++SF+RDEDERPKVAYN FSNEIP+ISL GID+ G+R+EIC+KIV+
Sbjct: 3 PSTLTALAEEKTLQTSFIRDEDERPKVAYNQFSNEIPIISLEGIDDETGKRAEICDKIVK 62
Query: 1131 ACENWGIFQVVDHGVDTELVSHMTTLAKEFFALPPEEKLRFDMTGGKKGGFIVSSHLQGE 952
ACE+WG+FQVVDHGVD EL+S MTT AKEFFALPPEEKLRFDM+GGKKGGFIVSSHLQGE
Sbjct: 63 ACEDWGVFQVVDHGVDAELISQMTTFAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQGE 122
Query: 951 SVQDWREIVTYFSYPIRNRDYSRWPDTPAGWKAVTEEYSEKLMGLACKLLEVLSEAMGLE 772
VQDWREIVTYFSYP R RDYSRWPD P GW AVT++YSEKLM LACKLL+VLSEAMGLE
Sbjct: 123 VVQDWREIVTYFSYPTRARDYSRWPDKPEGWIAVTQKYSEKLMELACKLLDVLSEAMGLE 182
Query: 771 KEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGK 592
KEALTKACVDMDQKVVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+DNGK
Sbjct: 183 KEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGK 242
Query: 591 TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYP 412
TWITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSNSSRLSIATFQNPAP+A VYP
Sbjct: 243 TWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVYP 302
Query: 411 LKVREGEKSVMEEPITFAEMYRRKMSKDIELARMKKLAKEKKLQ---DLEKAKLEPKPMN 241
LK+REGEKS+M+EPITFAEMYRRKMSKD+ELAR+KK AKE++LQ EKAKLE KP+
Sbjct: 303 LKIREGEKSIMDEPITFAEMYRRKMSKDLELARLKKQAKEQQLQAEVAAEKAKLESKPIE 362
Query: 240 EIFA 229
EI A
Sbjct: 363 EILA 366
>pir||A42110 flavanone 3 beta-hydroxylase - garden petunia (fragment)
Length = 369
Score = 633 bits (1632), Expect = e-180
Identities = 306/364 (84%), Positives = 337/364 (92%), Gaps = 3/364 (0%)
Frame = -3
Query: 1311 PKTLTTLAQQNTLESSFVRDEDERPKVAYNNFSNEIPVISLAGIDEVDGRRSEICNKIVE 1132
P TLT LA++ TL++SF+RDEDERPKVAYN FSNEIP+ISL GID+ G+R+EIC+KIV+
Sbjct: 6 PSTLTALAEEKTLQTSFIRDEDERPKVAYNQFSNEIPIISLEGIDDETGKRAEICDKIVK 65
Query: 1131 ACENWGIFQVVDHGVDTELVSHMTTLAKEFFALPPEEKLRFDMTGGKKGGFIVSSHLQGE 952
ACE+WG+FQVVDHGVD EL+S MTT AKEFFALPPEEKLRFDM+GGKKGGFIVSSHLQGE
Sbjct: 66 ACEDWGVFQVVDHGVDAELISQMTTFAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQGE 125
Query: 951 SVQDWREIVTYFSYPIRNRDYSRWPDTPAGWKAVTEEYSEKLMGLACKLLEVLSEAMGLE 772
VQDWREIVTYFSYP R RDYSRWPD P GW AVT++YSEKLM LACKLL+VLSEAMGLE
Sbjct: 126 VVQDWREIVTYFSYPTRARDYSRWPDKPEGWIAVTQKYSEKLMELACKLLDVLSEAMGLE 185
Query: 771 KEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGK 592
KEALTKACVDMDQKVVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+DNGK
Sbjct: 186 KEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGK 245
Query: 591 TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYP 412
TWITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSNSSRLSIATFQNPAP+A VYP
Sbjct: 246 TWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVYP 305
Query: 411 LKVREGEKSVMEEPITFAEMYRRKMSKDIELARMKKLAKEKKLQ---DLEKAKLEPKPMN 241
LK+REGEKS+M+EPITFAEMYRRKMSKD+ELAR+KK AKE++LQ EKAKLE KP+
Sbjct: 306 LKIREGEKSIMDEPITFAEMYRRKMSKDLELARLKKQAKEQQLQAEVAAEKAKLESKPIE 365
Query: 240 EIFA 229
EI A
Sbjct: 366 EILA 369
>sp|Q07353|FL3H_PETHY Naringenin,2-oxoglutarate 3-dioxygenase (Flavonone-3-hydroxylase)
(F3H) (FHT) gi|100398|pir||S16780 naringenin
3-dioxygenase (EC 1.14.11.9) - garden petunia
gi|1345562|emb|CAA43027.1| naringenin,2-oxoglutarate
3-dioxygenase [Petunia x hybrida]
Length = 369
Score = 632 bits (1629), Expect = e-180
Identities = 305/364 (83%), Positives = 337/364 (91%), Gaps = 3/364 (0%)
Frame = -3
Query: 1311 PKTLTTLAQQNTLESSFVRDEDERPKVAYNNFSNEIPVISLAGIDEVDGRRSEICNKIVE 1132
P TLT LA++ TL++SF+RDEDERPKVAYN FSNEIP+ISL GID+ G+R+EIC+KIV+
Sbjct: 6 PSTLTALAEEKTLQTSFIRDEDERPKVAYNQFSNEIPIISLEGIDDETGKRAEICDKIVK 65
Query: 1131 ACENWGIFQVVDHGVDTELVSHMTTLAKEFFALPPEEKLRFDMTGGKKGGFIVSSHLQGE 952
ACE+WG+FQVVDHGVD E++S MTT AKEFFALPPEEKLRFDM+GGKKGGFIVSSHLQGE
Sbjct: 66 ACEDWGVFQVVDHGVDAEVISQMTTFAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQGE 125
Query: 951 SVQDWREIVTYFSYPIRNRDYSRWPDTPAGWKAVTEEYSEKLMGLACKLLEVLSEAMGLE 772
VQDWREIVTYFSYP R RDYSRWPD P GW AVT++YSEKLM LACKLL+VLSEAMGLE
Sbjct: 126 VVQDWREIVTYFSYPTRARDYSRWPDKPEGWIAVTQKYSEKLMELACKLLDVLSEAMGLE 185
Query: 771 KEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGK 592
KEALTKACVDMDQKVVVN+YPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQAT+DNGK
Sbjct: 186 KEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGK 245
Query: 591 TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYP 412
TWITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSNSSRLSIATFQNPAP+A VYP
Sbjct: 246 TWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVYP 305
Query: 411 LKVREGEKSVMEEPITFAEMYRRKMSKDIELARMKKLAKEKKLQ---DLEKAKLEPKPMN 241
LK+REGEKS+M+EPITFAEMYRRKMSKD+ELAR+KK AKE++LQ EKAKLE KP+
Sbjct: 306 LKIREGEKSIMDEPITFAEMYRRKMSKDLELARLKKQAKEQQLQAEVAAEKAKLESKPIE 365
Query: 240 EIFA 229
EI A
Sbjct: 366 EILA 369
>gb|AAD56577.1|AF184270_1 flavanone 3-hydroxylase [Daucus carota]
Length = 366
Score = 629 bits (1623), Expect = e-179
Identities = 314/365 (86%), Positives = 333/365 (91%), Gaps = 4/365 (1%)
Frame = -3
Query: 1311 PKTLTTLAQQNTLESSFVRDEDERPKVAYNNFSNEIPVISLAGIDEVDGRRSEICNKIVE 1132
P TLT LAQ+ TL S FVRDEDERPKVAYN FS+EIPVISLAGID+ RRSEIC KIVE
Sbjct: 3 PSTLTALAQEKTLNSRFVRDEDERPKVAYNQFSDEIPVISLAGIDDA-ARRSEICRKIVE 61
Query: 1131 ACENWGIFQVVDHGVDTELVSHMTTLAKEFFALPPEEKLRFDMTGGKKGGFIVSSHLQGE 952
ACE+WGIFQVVDHGVDT L++ MT LA+EFFALP EKLRFDMTGGKKGGFIVSSHLQGE
Sbjct: 62 ACEDWGIFQVVDHGVDTALIAEMTRLAREFFALPAAEKLRFDMTGGKKGGFIVSSHLQGE 121
Query: 951 SVQDWREIVTYFSYPIRNRDYSRWPDTPAGWKAVTEEYSEKLMGLACKLLEVLSEAMGLE 772
+VQDWREIVTYFSYPIR RDYSRWPD P GW++ T EYSEKLMGL CKLLEVLSEAMGL+
Sbjct: 122 AVQDWREIVTYFSYPIRARDYSRWPDKPEGWRSATGEYSEKLMGLGCKLLEVLSEAMGLD 181
Query: 771 KEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGK 592
KEA+TKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD GK
Sbjct: 182 KEAITKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGK 241
Query: 591 TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYP 412
TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSN SRLSIATFQNPAP+ATVYP
Sbjct: 242 TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNYSRLSIATFQNPAPNATVYP 301
Query: 411 LKVREGEKSVMEEPITFAEMYRRKMSKDIELARMKKLAKEKKLQDLE--KAKLE--PKPM 244
LK+REGEK+VMEEPITFAEMYRRKMSKDIE+A +KKLAKEK LQD E KAKL+ PK
Sbjct: 302 LKIREGEKAVMEEPITFAEMYRRKMSKDIEVANLKKLAKEKLLQDQEWDKAKLQMKPKSA 361
Query: 243 NEIFA 229
+EIFA
Sbjct: 362 DEIFA 366
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,278,900,898
Number of Sequences: 1393205
Number of extensions: 31518676
Number of successful extensions: 126118
Number of sequences better than 10.0: 843
Number of HSP's better than 10.0 without gapping: 102124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121484
length of database: 448,689,247
effective HSP length: 127
effective length of database: 271,752,212
effective search space used: 88591221112
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
