>KMC002736A_C01 KMC002736A_c01
ggtcaaactcgttatattttatctgtgtataaattcagaatcaaaatacattgcctagct
agattgaatagtaatacatgGAACATTTTCCATACTGTCCCAAACAAAAAATAAGTCAAA
ATCTCAGTGCTTTTTTATTACTCTTCTTTGAACTTGCTTTGTGTATTGGCTTTACATGAT
TTCAGCGTATGCCAACCATGCTAACAAGATATAACAAACAGCACTCCAACAACAAACTGC
AAATCAACATGCAAGTTAGCTAACATCTGAGGCTGATCACAACTACAACAAAAGTGAAAA
GGGCTATTGTTCTACGTCTATACCAAGGGTCACCCCATCGCACGTTGTATCTTGCTGCAA
GATAGTGAGCACCGACAGGACCCCGACTACCATAAGGATAGGTACTCTGGAATCACTTTT
TTCTCTTCTAACTCATTCAGTAGAGGCGTGAAGAGTGACCAAGCAGCATCAAGTTCATCA
CTGCGAATGAAGAGTCTTCTTTCCCCTTCAATCGCGTCCAGAAGTAGCCTCTCGTAAGCG
TCTGGAATCTCCTTCGCGTATCTGGCTGCATAGTGAAGATTCAGATGACTGCGATCCAAC
CTCATTTCTAGACCCGGCACTTTATGGTTGATCTTCAAATAAATAGCCTCATCAGGCTGA
ACTCTTATAACAAGTTCATTTGTAGCTCGATCAAGAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002736A_C01 KMC002736A_c01
         (697 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAB52708.1| glucose-6-phosphate 1-dehydrogenase [Solanum tub...   173  2e-53
sp|Q43793|G6PC_TOBAC Glucose-6-phosphate 1-dehydrogenase, chloro...   174  4e-53
dbj|BAC23041.1| glucose 6-phosphate dehydrogenase [Solanum tuber...   171  1e-52
ref|NP_196815.2| glucose-6-phosphate 1-dehydrogenase; protein id...   175  2e-52
emb|CAC05439.1| glucose-6-phosphate 1-dehydrogenase [Arabidopsis...   175  2e-52

>emb|CAB52708.1| glucose-6-phosphate 1-dehydrogenase [Solanum tuberosum]
          Length = 582

 Score =  173 bits (439), Expect(2) = 2e-53
 Identities = 86/97 (88%), Positives = 93/97 (95%)
 Frame = -3

Query: 695 LDRATNELVIRVQPDEAIYLKINHKVPGLEMRLDRSHLNLHYAARYAKEIPDAYERLLLD 516
           LD+ATNELVIRVQPDEAIYLKIN+KVPGL MRLDRS+LNL Y+ARYAKEIPDAYERLLLD
Sbjct: 459 LDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLLYSARYAKEIPDAYERLLLD 518

Query: 515 AIEGERRLFIRSDELDAAWSLFTPLLNELEEKKVIPE 405
           AIEGERRLFIRSDELDAAWSLFTP+L ELE KK++PE
Sbjct: 519 AIEGERRLFIRSDELDAAWSLFTPVLKELEHKKIVPE 555

 Score = 58.2 bits (139), Expect(2) = 2e-53
 Identities = 24/33 (72%), Positives = 27/33 (81%)
 Frame = -1

Query: 427 KRKK*FQSTYPYGSRGPVGAHYLAARYNVRWGD 329
           + KK    +YPYGSRGP+GAHYLAARY VRWGD
Sbjct: 548 EHKKIVPESYPYGSRGPIGAHYLAARYKVRWGD 580

>sp|Q43793|G6PC_TOBAC Glucose-6-phosphate 1-dehydrogenase, chloroplast precursor (G6PD)
           gi|7431273|pir||T03244 glucose-6-phosphate
           1-dehydrogenase (EC 1.1.1.49) - common tobacco
           gi|1480344|emb|CAA67782.1| glucose-6-phosphate
           dehydrogenase [Nicotiana tabacum]
          Length = 593

 Score =  174 bits (440), Expect(2) = 4e-53
 Identities = 85/98 (86%), Positives = 95/98 (96%)
 Frame = -3

Query: 695 LDRATNELVIRVQPDEAIYLKINHKVPGLEMRLDRSHLNLHYAARYAKEIPDAYERLLLD 516
           LD+ATNELVIRVQP+EAIYLKIN+KVPGL MRLDRS+LNL Y+ARY+KEIPDAYERLLLD
Sbjct: 470 LDQATNELVIRVQPNEAIYLKINNKVPGLGMRLDRSNLNLLYSARYSKEIPDAYERLLLD 529

Query: 515 AIEGERRLFIRSDELDAAWSLFTPLLNELEEKKVIPEY 402
           AIEGERRLFIRSDELDAAWSLFTP+L ELE+KK++PEY
Sbjct: 530 AIEGERRLFIRSDELDAAWSLFTPVLKELEDKKIVPEY 567

 Score = 56.6 bits (135), Expect(2) = 4e-53
 Identities = 24/31 (77%), Positives = 25/31 (80%)
 Frame = -1

Query: 421 KK*FQSTYPYGSRGPVGAHYLAARYNVRWGD 329
           KK     YPYGSRGP+GAHYLAARY VRWGD
Sbjct: 561 KKIVPEYYPYGSRGPIGAHYLAARYKVRWGD 591

>dbj|BAC23041.1| glucose 6-phosphate dehydrogenase [Solanum tuberosum]
          Length = 581

 Score =  171 bits (433), Expect(2) = 1e-52
 Identities = 84/97 (86%), Positives = 93/97 (95%)
 Frame = -3

Query: 695 LDRATNELVIRVQPDEAIYLKINHKVPGLEMRLDRSHLNLHYAARYAKEIPDAYERLLLD 516
           LD+ATNELVIRVQPDEAIYLKIN+KVPGL MRLDRS+LNL Y+ARY+KEIPDAYERLLLD
Sbjct: 458 LDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLLYSARYSKEIPDAYERLLLD 517

Query: 515 AIEGERRLFIRSDELDAAWSLFTPLLNELEEKKVIPE 405
           AIEGERRLFIRSDELDAAWSLFTP+L +LE KK++PE
Sbjct: 518 AIEGERRLFIRSDELDAAWSLFTPVLKDLEHKKIVPE 554

 Score = 58.2 bits (139), Expect(2) = 1e-52
 Identities = 24/33 (72%), Positives = 27/33 (81%)
 Frame = -1

Query: 427 KRKK*FQSTYPYGSRGPVGAHYLAARYNVRWGD 329
           + KK    +YPYGSRGP+GAHYLAARY VRWGD
Sbjct: 547 EHKKIVPESYPYGSRGPIGAHYLAARYKVRWGD 579

>ref|NP_196815.2| glucose-6-phosphate 1-dehydrogenase; protein id: At5g13110.1,
           supported by cDNA: gi_18086447 [Arabidopsis thaliana]
           gi|25452980|sp|Q9FY99|GPD2_ARATH Glucose-6-phosphate
           1-dehydrogenase 2, chloroplast precursor (G6PD2)
           (G6PDH2)
          Length = 596

 Score =  175 bits (444), Expect(2) = 2e-52
 Identities = 88/98 (89%), Positives = 94/98 (95%)
 Frame = -3

Query: 695 LDRATNELVIRVQPDEAIYLKINHKVPGLEMRLDRSHLNLHYAARYAKEIPDAYERLLLD 516
           LD+ATNELVIRVQPDEAIYLKIN+KVPGL MRLDRS+LNL Y+ARY+KEIPDAYERLLLD
Sbjct: 470 LDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLLYSARYSKEIPDAYERLLLD 529

Query: 515 AIEGERRLFIRSDELDAAWSLFTPLLNELEEKKVIPEY 402
           AIEGERRLFIRSDELDAAWSLFTPLL E+EEKK IPEY
Sbjct: 530 AIEGERRLFIRSDELDAAWSLFTPLLKEIEEKKRIPEY 567

 Score = 53.1 bits (126), Expect(2) = 2e-52
 Identities = 22/33 (66%), Positives = 26/33 (78%)
 Frame = -1

Query: 427 KRKK*FQSTYPYGSRGPVGAHYLAARYNVRWGD 329
           + KK     YPYGSRGPVGAHYLAA++ V+WGD
Sbjct: 559 EEKKRIPEYYPYGSRGPVGAHYLAAKHKVQWGD 591

>emb|CAC05439.1| glucose-6-phosphate 1-dehydrogenase [Arabidopsis thaliana]
          Length = 593

 Score =  175 bits (444), Expect(2) = 2e-52
 Identities = 88/98 (89%), Positives = 94/98 (95%)
 Frame = -3

Query: 695 LDRATNELVIRVQPDEAIYLKINHKVPGLEMRLDRSHLNLHYAARYAKEIPDAYERLLLD 516
           LD+ATNELVIRVQPDEAIYLKIN+KVPGL MRLDRS+LNL Y+ARY+KEIPDAYERLLLD
Sbjct: 467 LDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLLYSARYSKEIPDAYERLLLD 526

Query: 515 AIEGERRLFIRSDELDAAWSLFTPLLNELEEKKVIPEY 402
           AIEGERRLFIRSDELDAAWSLFTPLL E+EEKK IPEY
Sbjct: 527 AIEGERRLFIRSDELDAAWSLFTPLLKEIEEKKRIPEY 564

 Score = 53.1 bits (126), Expect(2) = 2e-52
 Identities = 22/33 (66%), Positives = 26/33 (78%)
 Frame = -1

Query: 427 KRKK*FQSTYPYGSRGPVGAHYLAARYNVRWGD 329
           + KK     YPYGSRGPVGAHYLAA++ V+WGD
Sbjct: 556 EEKKRIPEYYPYGSRGPVGAHYLAAKHKVQWGD 588

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 544,592,386
Number of Sequences: 1393205
Number of extensions: 11066957
Number of successful extensions: 27879
Number of sequences better than 10.0: 278
Number of HSP's better than 10.0 without gapping: 26633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27706
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31684559424
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR081b02_f BP082204 1 384 2 GNf071e10 BP072630 112 518 3 GNf019h03 BP068779 113 560 4 MFB019a01_f BP035303 113 665 5 GNf034g05 BP069864 113 537 6 MR015a08_f BP077078 113 166 7 MR083a05_f BP082354 114 344 8 MPDL088h04_f AV781126 114 656 9 MR089a08_f BP082812 116 596 10 GNLf006g10 BP075174 137 704

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