KMC002709A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002709A_C01 KMC002709A_c01
gtttttttttaccaaactcaactcatatgcacccctaattCAAATTATTTGTTTACATAT
ATAAATATATAATATAACAACAAGCCAAGATCGAAGGCCTAATATTCTGCTCCTTCTGTA
CATATATATAGATTAATATAAACTACATAGAGTGGTTCAAAATTCACACACTAGCTAGCT
AGTAGACCTAGTTGGTGGAGAATGTAGTGCATGACTGAGGAAGTTGTGGTGAACCGGGAA
TAATAGCACTTCCATTATTGCTATTGTTATTAACATCACTACTACTGTTATTATGACCAT
CACTCCCTCTTATGATTGATGAGATTGCTGCTGCCAATGCTGCAGTGAAGTTCGGATCCG
AAGCAATGGCTGCACTCATTGTTTCAACCGTTGGTGGTCGTTGTCCTAGT


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002709A_C01 KMC002709A_c01
         (410 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T02003 probable DNA-binding protein T15B16.12 - Arabidopsis...    82  2e-15
ref|NP_192081.1| WRKY family transcription factor; protein id: A...    82  2e-15
pir||T49114 hypothetical protein AT4g22070 - Arabidopsis thalian...    52  1e-06
ref|NP_567644.1| WRKY family transcription factor; protein id: A...    52  1e-06
dbj|BAA96574.1| unnamed protein product [Oryza sativa (japonica ...    52  2e-06

>pir||T02003 probable DNA-binding protein T15B16.12 - Arabidopsis thaliana
           gi|3859603|gb|AAC72869.1| contains similarity to wild
           oat DNA-binding protein ABF2 (GB:Z48431) [Arabidopsis
           thaliana]
          Length = 403

 Score = 82.0 bits (201), Expect = 2e-15
 Identities = 44/76 (57%), Positives = 57/76 (74%), Gaps = 5/76 (6%)
 Frame = -2

Query: 403 QRPPT--VETMSAAIASDPNFTAALAAAISSIIRGSDGHNNSSSDVNNN---SNNGSAII 239
           Q PP   V+++ AAIA DPNFTAALAAAIS+II G +  NN+++D+N+N   + +G +  
Sbjct: 328 QAPPREMVDSVRAAIAMDPNFTAALAAAISNIIGGGNNDNNNNTDINDNKVDAKSGGSSN 387

Query: 238 PGSPQLPQSCTTFSTN 191
             SPQLPQSCTTFSTN
Sbjct: 388 GDSPQLPQSCTTFSTN 403

>ref|NP_192081.1| WRKY family transcription factor; protein id: At4g01720.1,
           supported by cDNA: gi_19172391 [Arabidopsis thaliana]
           gi|20978798|sp|Q9ZSI7|WR47_ARATH Probable WRKY
           transcription factor 47 (WRKY DNA-binding protein 47)
           gi|25407124|pir||B85022 probable DNA-binding protein
           [imported] - Arabidopsis thaliana
           gi|7268215|emb|CAB77742.1| putative DNA-binding protein
           [Arabidopsis thaliana]
           gi|19172392|gb|AAL85881.1|AF480165_1 WRKY transcription
           factor 47 [Arabidopsis thaliana]
          Length = 489

 Score = 82.0 bits (201), Expect = 2e-15
 Identities = 44/76 (57%), Positives = 57/76 (74%), Gaps = 5/76 (6%)
 Frame = -2

Query: 403 QRPPT--VETMSAAIASDPNFTAALAAAISSIIRGSDGHNNSSSDVNNN---SNNGSAII 239
           Q PP   V+++ AAIA DPNFTAALAAAIS+II G +  NN+++D+N+N   + +G +  
Sbjct: 414 QAPPREMVDSVRAAIAMDPNFTAALAAAISNIIGGGNNDNNNNTDINDNKVDAKSGGSSN 473

Query: 238 PGSPQLPQSCTTFSTN 191
             SPQLPQSCTTFSTN
Sbjct: 474 GDSPQLPQSCTTFSTN 489

>pir||T49114 hypothetical protein AT4g22070 - Arabidopsis thaliana
           gi|2961352|emb|CAA18110.1| putative protein [Arabidopsis
           thaliana] gi|7269052|emb|CAB79162.1| putative protein
           [Arabidopsis thaliana]
          Length = 458

 Score = 52.4 bits (124), Expect = 1e-06
 Identities = 31/51 (60%), Positives = 37/51 (71%), Gaps = 3/51 (5%)
 Frame = -2

Query: 391 TVETMSAAIASDPNFTAALAAAISSIIRGSDGHNNSSSDVNNN---SNNGS 248
           +V   SAAIASDPNF AALAAAI+SI+ GS   NN+++  NNN   SNN S
Sbjct: 408 SVSAASAAIASDPNFAAALAAAITSIMNGSSHQNNNTN--NNNVATSNNDS 456

>ref|NP_567644.1| WRKY family transcription factor; protein id: At4g22070.1,
           supported by cDNA: gi_15990589 [Arabidopsis thaliana]
           gi|20978775|sp|Q93WT0|WR31_ARATH Probable WRKY
           transcription factor 31 (WRKY DNA-binding protein 31)
           gi|15990590|gb|AAL11009.1| WRKY transcription factor 31
           [Arabidopsis thaliana]
          Length = 538

 Score = 52.4 bits (124), Expect = 1e-06
 Identities = 31/51 (60%), Positives = 37/51 (71%), Gaps = 3/51 (5%)
 Frame = -2

Query: 391 TVETMSAAIASDPNFTAALAAAISSIIRGSDGHNNSSSDVNNN---SNNGS 248
           +V   SAAIASDPNF AALAAAI+SI+ GS   NN+++  NNN   SNN S
Sbjct: 488 SVSAASAAIASDPNFAAALAAAITSIMNGSSHQNNNTN--NNNVATSNNDS 536

>dbj|BAA96574.1| unnamed protein product [Oryza sativa (japonica cultivar-group)]
          Length = 594

 Score = 52.0 bits (123), Expect = 2e-06
 Identities = 27/68 (39%), Positives = 42/68 (61%), Gaps = 10/68 (14%)
 Frame = -2

Query: 403 QRPPTVETMSAAIASDPNFTAALAAAISSIIRGSDGHNNSSS----------DVNNNSNN 254
           ++P  +ET++AA+A+DPNFT ALAAAISS++ G   H   S+          D N N ++
Sbjct: 499 RQPSVMETVTAALAADPNFTTALAAAISSVVAGGAHHQALSTTPRGSAAGAGDGNGNGSS 558

Query: 253 GSAIIPGS 230
            +A+  G+
Sbjct: 559 AAAVATGA 566

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 340,890,299
Number of Sequences: 1393205
Number of extensions: 6888401
Number of successful extensions: 70364
Number of sequences better than 10.0: 195
Number of HSP's better than 10.0 without gapping: 22371
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 54388
length of database: 448,689,247
effective HSP length: 112
effective length of database: 292,650,287
effective search space used: 7023606888
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GNf056a10 BP071515 1 415
2 GNf055b04 BP071443 41 405
3 GNLf003c05 BP074953 42 365
4 GNLf008g07 AU254047 72 311




Lotus japonicus
Kazusa DNA Research Institute