>KMC002632A_C01 KMC002632A_c01
aaggaaacaaacgAAAGGCTATTAAAAAAAACGAAAAGTTTTAATGTATCAAGTCAAAAC
AGAACGCAAGAGTTACTTTCAAACATATATCTACAGACACAAGACCTTGATATACCACCT
ATGGGGGAGGGAGTATTATTCAAACTTTTCTTTACAGACTGAGGCTACAAAATGAAGTAA
TATTCTCCTCTACCTCTTGAGTGGTTTGTAAAGGACAACTGTGACATATTTGGACTGGAA
CCTGTGAGTCATTCCTAGGGCAACAACAGACTTTGACGCCATGTTTTTCTCTATGAAGAC
ATGATTTAGCACAACATGTTGAGGCTTTGAAGAGGAAACCACTTCAGTTGCATTCTCCAC
ACCTAGGACTGTAAGATGTAGCTGCGAAGGAACGGCCATTGGCTCTTTTGCATAGTCCTC
ATCAGGTGGAAAGTCATGACCGTAGCTGGATTGTGGGGAGGGTGGCGCCTCAAACTCAGA
CACGCCTTCAGGGTTTTCTGGCACGTAATCATTGACATCAAGAAGATTGTAGACATGCCC
CATCTCATCAGTAACATAAGGAAGCTCTGGAATATATCTCTGT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002632A_C01 KMC002632A_c01
         (583 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAB52141.1| GAL83 protein [Solanum tuberosum]                     196  1e-49
ref|NP_197615.1| AKIN beta1; protein id: At5g21170.1, supported ...   181  6e-45
emb|CAB64718.1| AKIN gamma [Arabidopsis thaliana] gi|21593717|gb...   181  6e-45
gb|AAG41995.1|AF322108_1 SNF1-related kinase complex anchoring p...   135  4e-31
emb|CAB64719.1| AKIN beta2 [Arabidopsis thaliana] gi|13430672|gb...   134  6e-31

>emb|CAB52141.1| GAL83 protein [Solanum tuberosum]
          Length = 289

 Score =  196 bits (499), Expect = 1e-49
 Identities = 99/129 (76%), Positives = 107/129 (82%)
 Frame = -2

Query: 579 RYIPELPYVTDEMGHVYNLLDVNDYVPENPEGVSEFEAPPSPQSSYGHDFPPDEDYAKEP 400
           RYIPELP V DE G V+NLLDVND VPEN E V+EFEAPPSP SSY      DED+AKEP
Sbjct: 161 RYIPELPCVADETGVVFNLLDVNDNVPENLESVAEFEAPPSPDSSYAQALLVDEDFAKEP 220

Query: 399 MAVPSQLHLTVLGVENATEVVSSSKPQHVVLNHVFIEKNMASKSVVALGMTHRFQSKYVT 220
           +AVP QLHLTVLG EN+ E  SS KPQHVVLNH+FIEK  AS+SVVALG+THRFQSKYVT
Sbjct: 221 VAVPPQLHLTVLGSENSEEAPSSPKPQHVVLNHLFIEKGWASQSVVALGLTHRFQSKYVT 280

Query: 219 VVLYKPLKR 193
           VVLYKPLKR
Sbjct: 281 VVLYKPLKR 289

>ref|NP_197615.1| AKIN beta1; protein id: At5g21170.1, supported by cDNA: 41668.
           [Arabidopsis thaliana] gi|29294057|gb|AAO73894.1| AMPKBI
           (5'-AMP-activated protein kinase, beta subunit,
           complex-interacting region) domain family [Arabidopsis
           thaliana]
          Length = 283

 Score =  181 bits (459), Expect = 6e-45
 Identities = 84/129 (65%), Positives = 105/129 (81%)
 Frame = -2

Query: 579 RYIPELPYVTDEMGHVYNLLDVNDYVPENPEGVSEFEAPPSPQSSYGHDFPPDEDYAKEP 400
           +YIP+LP+V DE+G+V N+LDV+++VPENPE + EFEAPPSP  SYG   P  EDYAKEP
Sbjct: 157 KYIPDLPFVADEVGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEP 216

Query: 399 MAVPSQLHLTVLGVENATEVVSSSKPQHVVLNHVFIEKNMASKSVVALGMTHRFQSKYVT 220
           +AVP QLHLT+LG    T +  ++KPQHVVLNHVFIE+    +S+VALG+THRF+SKY+T
Sbjct: 217 LAVPPQLHLTLLGTTEETAI--ATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYIT 274

Query: 219 VVLYKPLKR 193
           VVLYKPL R
Sbjct: 275 VVLYKPLTR 283

>emb|CAB64718.1| AKIN gamma [Arabidopsis thaliana] gi|21593717|gb|AAM65684.1| AKIN
           beta1 [Arabidopsis thaliana]
          Length = 284

 Score =  181 bits (459), Expect = 6e-45
 Identities = 84/129 (65%), Positives = 105/129 (81%)
 Frame = -2

Query: 579 RYIPELPYVTDEMGHVYNLLDVNDYVPENPEGVSEFEAPPSPQSSYGHDFPPDEDYAKEP 400
           +YIP+LP+V DE+G+V N+LDV+++VPENPE + EFEAPPSP  SYG   P  EDYAKEP
Sbjct: 158 KYIPDLPFVADEVGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEP 217

Query: 399 MAVPSQLHLTVLGVENATEVVSSSKPQHVVLNHVFIEKNMASKSVVALGMTHRFQSKYVT 220
           +AVP QLHLT+LG    T +  ++KPQHVVLNHVFIE+    +S+VALG+THRF+SKY+T
Sbjct: 218 LAVPPQLHLTLLGTTEETAI--ATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYIT 275

Query: 219 VVLYKPLKR 193
           VVLYKPL R
Sbjct: 276 VVLYKPLTR 284

>gb|AAG41995.1|AF322108_1 SNF1-related kinase complex anchoring protein SIP1 [Lycopersicon
           esculentum]
          Length = 227

 Score =  135 bits (340), Expect = 4e-31
 Identities = 70/131 (53%), Positives = 91/131 (69%), Gaps = 2/131 (1%)
 Frame = -2

Query: 579 RYIPELPYVTDEMGHVYNLLDVNDYVPENPEGVSEFEAPPSPQSSYGHDFPPDEDYAKEP 400
           R  P+LP V DE G+ YNLLD+ DYVPE+ E +  FE P SP SSY +     EDYAKEP
Sbjct: 97  RCSPDLPCVQDEAGNTYNLLDMKDYVPEDIESIYGFEPPQSPDSSYNNLHLVSEDYAKEP 156

Query: 399 MAVPSQLHLTVLGVENATEVVSS--SKPQHVVLNHVFIEKNMASKSVVALGMTHRFQSKY 226
             VP  L +T+L V  +   +    S+PQHVVLNH++++K+ ++ SVVALG T+RF SKY
Sbjct: 157 PVVPPHLQMTLLNVSPSHMEIPPPLSRPQHVVLNHLYMQKDRSTPSVVALGSTNRFLSKY 216

Query: 225 VTVVLYKPLKR 193
           VTVVLYK ++R
Sbjct: 217 VTVVLYKSIQR 227

>emb|CAB64719.1| AKIN beta2 [Arabidopsis thaliana]
           gi|13430672|gb|AAK25958.1|AF360248_1 putative kinase
           [Arabidopsis thaliana]
          Length = 289

 Score =  134 bits (338), Expect = 6e-31
 Identities = 65/131 (49%), Positives = 91/131 (68%), Gaps = 2/131 (1%)
 Frame = -2

Query: 579 RYIPELPYVTDEMGHVYNLLDVNDYVPENPEGVSEFEAPPSPQSSYGHDFPPDEDYAKEP 400
           R+ PELP   D+ G+ +N+LD+ DYVPE+ + +S FE P SP++SY +     EDY+KEP
Sbjct: 159 RHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEP 218

Query: 399 MAVPSQLHLTVLGVENATEVVSS--SKPQHVVLNHVFIEKNMASKSVVALGMTHRFQSKY 226
             VP  L +T+L +  A   + S   +PQHV+LNH++++K  +  SVVALG THRF +KY
Sbjct: 219 PVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKY 278

Query: 225 VTVVLYKPLKR 193
           VTVVLYK L+R
Sbjct: 279 VTVVLYKSLQR 289

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 497,688,453
Number of Sequences: 1393205
Number of extensions: 11331372
Number of successful extensions: 32261
Number of sequences better than 10.0: 92
Number of HSP's better than 10.0 without gapping: 29963
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32136
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENf088b04 BP062067 1 495 2 MPD098c06_f AV776393 14 428 3 GENf091a06 BP062154 17 498 4 MPD022g12_f AV771541 21 496 5 GNf022a03 BP068936 22 118 6 MFB056b06_f BP038035 23 523 7 SPD022c06_f BP045717 24 556 8 MR033h04_f BP078578 24 423 9 SPD015a09_f BP045161 24 522 10 GNf068c10 BP072404 25 377 11 MPD008h06_f AV770558 26 591 12 GNf030c06 BP069528 43 403 13 MR096g06_f BP083382 49 518

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