Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002581A_C03 KMC002581A_c03
(1054 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T52558 translation elongation factor eEF1Balpha (clone 2) [... 234 3e-81
ref|NP_196772.1| elongation factor 1B alpha-subunit (emb|CAB6472... 221 6e-81
ref|NP_568375.1| elongation factor 1B alpha-subunit; protein id:... 222 2e-77
sp|P29545|EF1D_ORYSA ELONGATION FACTOR 1-BETA' (EF-1-BETA') gi|3... 216 6e-76
dbj|BAC22427.1| putative translation elongation factor eEF-1 bet... 219 5e-74
>pir||T52558 translation elongation factor eEF1Balpha (clone 2) [validated] -
Arabidopsis thaliana gi|6686821|emb|CAB64730.1|
elongation factor 1B alpha-subunit [Arabidopsis
thaliana] gi|13430784|gb|AAK26014.1|AF360304_1 putative
elongation factor 1B alpha-subunit [Arabidopsis
thaliana] gi|15810631|gb|AAL07240.1| putative elongation
factor 1B alpha-subunit [Arabidopsis thaliana]
Length = 224
Score = 234 bits (596), Expect(2) = 3e-81
Identities = 125/169 (73%), Positives = 134/169 (78%), Gaps = 1/169 (0%)
Frame = -3
Query: 821 LAASFPGNAQGVRFSGKAAAKLDASAEDDLNKDFQKVSLDTAEDDDDLDLFGDETEEDKK 642
LA SFPG A GV+F G AAA AE A+DDDD+DLFGDETEE+KK
Sbjct: 65 LAKSFPGKAVGVQFGGSAAAAPAVEAE---------APAAAADDDDDMDLFGDETEEEKK 115
Query: 641 AAEEREAAKKPAKK-KESGKSSVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASKLVA 465
AAEEREAAKK KK KESGKSSVL+DVKPWDDETDMKKLEEAVR VEMPGLFWGASKLV
Sbjct: 116 AAEEREAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVP 175
Query: 464 VGYGIKKLQIMLTIVDDLVSVDTLIEECLTAEPRDEFIQSCDIVAFNKI 318
VGYGIKKL IM TIVDDLVS D LIE+ LT+EP +E+IQSCDIVAFNKI
Sbjct: 176 VGYGIKKLTIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVAFNKI 224
Score = 91.7 bits (226), Expect(2) = 3e-81
Identities = 40/70 (57%), Positives = 54/70 (77%)
Frame = -2
Query: 1014 MAVTFSNLHTDSGLESLDGHLSGKTYVSGDQFTKDDIKVFGFVLEKPGDNFPNAGKWYDA 835
MAVTFS+LHT+ G++S++ HL+GKTY+SGDQ + DD+KV+ V KP D FPNA KWY++
Sbjct: 1 MAVTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYES 60
Query: 834 VSSHLGCQLP 805
V+S L P
Sbjct: 61 VASQLAKSFP 70
>ref|NP_196772.1| elongation factor 1B alpha-subunit (emb|CAB64729.1); protein id:
At5g12110.1 [Arabidopsis thaliana]
gi|25456755|pir||T52559 translation elongation factor
eEF1Balpha (clone 1) [validated] - Arabidopsis thaliana
gi|6686819|emb|CAB64729.1| elongation factor 1B
alpha-subunit [Arabidopsis thaliana]
gi|9759378|dbj|BAB10029.1| elongation factor 1B
alpha-subunit [Arabidopsis thaliana]
Length = 228
Score = 221 bits (562), Expect(2) = 6e-81
Identities = 118/169 (69%), Positives = 133/169 (77%), Gaps = 1/169 (0%)
Frame = -3
Query: 821 LAASFPGNAQGVRFSGKAAAKLDASAEDDLNKDFQKVSLDTAEDDDDLDLFGDETEEDKK 642
LA SFPG A GVR G A +A + + + +DDDD+DLF DETE++KK
Sbjct: 65 LAKSFPGKADGVRVGGGVAPPSEAHPHTE-----EPAADGDGDDDDDIDLFADETEDEKK 119
Query: 641 AAEEREAAKKPAKK-KESGKSSVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASKLVA 465
AAEEREAAKK KK KESGKSSVLL+VKPWDDETDMKKLEEAVRSV+MPGL WGASKLV
Sbjct: 120 AAEEREAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVP 179
Query: 464 VGYGIKKLQIMLTIVDDLVSVDTLIEECLTAEPRDEFIQSCDIVAFNKI 318
VGYGIKKL IM+TIVDDLVSVD LIE+ LT+EP +E+IQS DIVAFNKI
Sbjct: 180 VGYGIKKLTIMMTIVDDLVSVDNLIEDHLTSEPNNEYIQSVDIVAFNKI 228
Score = 103 bits (257), Expect(2) = 6e-81
Identities = 45/70 (64%), Positives = 57/70 (81%)
Frame = -2
Query: 1014 MAVTFSNLHTDSGLESLDGHLSGKTYVSGDQFTKDDIKVFGFVLEKPGDNFPNAGKWYDA 835
MAVTFS+LHT+ GL++L+ HL+GKTY+SGDQ + DD+KV+ VLE PGD FPNA KWYD+
Sbjct: 1 MAVTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDS 60
Query: 834 VSSHLGCQLP 805
V+SHL P
Sbjct: 61 VASHLAKSFP 70
>ref|NP_568375.1| elongation factor 1B alpha-subunit; protein id: At5g19510.1,
supported by cDNA: 26936. [Arabidopsis thaliana]
Length = 221
Score = 222 bits (566), Expect(2) = 2e-77
Identities = 119/161 (73%), Positives = 128/161 (78%), Gaps = 1/161 (0%)
Frame = -3
Query: 797 AQGVRFSGKAAAKLDASAEDDLNKDFQKVSLDTAEDDDDLDLFGDETEEDKKAAEEREAA 618
A GV+F G AAA AE A+DDDD+DLFGDETEE+KKAAEEREAA
Sbjct: 70 AVGVQFGGSAAAAPAVEAE---------APAAAADDDDDMDLFGDETEEEKKAAEEREAA 120
Query: 617 KKPAKK-KESGKSSVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASKLVAVGYGIKKL 441
KK KK KESGKSSVL+DVKPWDDETDMKKLEEAVR VEMPGLFWGASKLV VGYGIKKL
Sbjct: 121 KKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGIKKL 180
Query: 440 QIMLTIVDDLVSVDTLIEECLTAEPRDEFIQSCDIVAFNKI 318
IM TIVDDLVS D LIE+ LT+EP +E+IQSCDIVAFNKI
Sbjct: 181 TIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVAFNKI 221
Score = 90.1 bits (222), Expect(2) = 2e-77
Identities = 39/65 (60%), Positives = 53/65 (81%)
Frame = -2
Query: 1014 MAVTFSNLHTDSGLESLDGHLSGKTYVSGDQFTKDDIKVFGFVLEKPGDNFPNAGKWYDA 835
MAVTFS+LHT+ G++S++ HL+GKTY+SGDQ + DD+KV+ V KP D FPNA KWY++
Sbjct: 1 MAVTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYES 60
Query: 834 VSSHL 820
V+S L
Sbjct: 61 VASQL 65
>sp|P29545|EF1D_ORYSA ELONGATION FACTOR 1-BETA' (EF-1-BETA') gi|322851|pir||S29224
translation elongation factor eEF-1 beta' chain - rice
gi|218161|dbj|BAA02253.1| elongation factor 1 beta'
[Oryza sativa (japonica cultivar-group)]
Length = 223
Score = 216 bits (550), Expect(2) = 6e-76
Identities = 119/168 (70%), Positives = 128/168 (75%)
Frame = -3
Query: 821 LAASFPGNAQGVRFSGKAAAKLDASAEDDLNKDFQKVSLDTAEDDDDLDLFGDETEEDKK 642
LA+ FPG A GV G AA A+A + D EDDDDLDLFGDETEEDKK
Sbjct: 65 LASRFPGKAVGVNLPGGGAASSAAAAAP--------AAKDADEDDDDLDLFGDETEEDKK 116
Query: 641 AAEEREAAKKPAKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASKLVAV 462
AA+ER A+K +KKKESGKSSVLLDVKPWDDETDMKKLEEAVRSV+M GL WGASKLV V
Sbjct: 117 AADERAASKASSKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVQMEGLTWGASKLVPV 176
Query: 461 GYGIKKLQIMLTIVDDLVSVDTLIEECLTAEPRDEFIQSCDIVAFNKI 318
GYGIKKLQIMLTIVDDLVSV LT EP +EF+QSCDIVAFNKI
Sbjct: 177 GYGIKKLQIMLTIVDDLVSV-IAYRRHLTEEPINEFVQSCDIVAFNKI 223
Score = 91.7 bits (226), Expect(2) = 6e-76
Identities = 42/70 (60%), Positives = 53/70 (75%)
Frame = -2
Query: 1014 MAVTFSNLHTDSGLESLDGHLSGKTYVSGDQFTKDDIKVFGFVLEKPGDNFPNAGKWYDA 835
MAVTF++LHT GL++L+ HLSGKTYVSG+ +KDDIKVF V KPG FPNA +WYD
Sbjct: 1 MAVTFTDLHTADGLKALEQHLSGKTYVSGNAISKDDIKVFAAVPSKPGAEFPNAARWYDT 60
Query: 834 VSSHLGCQLP 805
V++ L + P
Sbjct: 61 VAAALASRFP 70
>dbj|BAC22427.1| putative translation elongation factor eEF-1 beta' chain [Oryza
sativa (japonica cultivar-group)]
Length = 220
Score = 219 bits (558), Expect(2) = 5e-74
Identities = 122/166 (73%), Positives = 131/166 (78%)
Frame = -3
Query: 815 ASFPGNAQGVRFSGKAAAKLDASAEDDLNKDFQKVSLDTAEDDDDLDLFGDETEEDKKAA 636
ASFPG A GV G AA A+A + D EDDDDLDLFGDETEEDKKAA
Sbjct: 65 ASFPGKAVGVNLPGGGAASSAAAAAP--------AAKDADEDDDDLDLFGDETEEDKKAA 116
Query: 635 EEREAAKKPAKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASKLVAVGY 456
+ER A+K +KKKE GKSSVLLDVKPWDDETDMKKLEEAVRSV+M GL WGA KLV VGY
Sbjct: 117 DERAASKASSKKKE-GKSSVLLDVKPWDDETDMKKLEEAVRSVQMEGLTWGA-KLVPVGY 174
Query: 455 GIKKLQIMLTIVDDLVSVDTLIEECLTAEPRDEFIQSCDIVAFNKI 318
GIKKLQIMLTIVDDLVSVD+LIEE LT EP +EF+QSCDIVAFNKI
Sbjct: 175 GIKKLQIMLTIVDDLVSVDSLIEEHLTEEPINEFVQSCDIVAFNKI 220
Score = 82.0 bits (201), Expect(2) = 5e-74
Identities = 40/65 (61%), Positives = 50/65 (76%)
Frame = -2
Query: 1014 MAVTFSNLHTDSGLESLDGHLSGKTYVSGDQFTKDDIKVFGFVLEKPGDNFPNAGKWYDA 835
MAVTF++LHT GL++L+ HLSGKTYVS + +KDDIKVF V KPG FPNA +WYD
Sbjct: 1 MAVTFTDLHTADGLKALEQHLSGKTYVS-NAISKDDIKVFAAVPSKPGAEFPNAARWYDT 59
Query: 834 VSSHL 820
V++ L
Sbjct: 60 VAAAL 64
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 961,821,667
Number of Sequences: 1393205
Number of extensions: 22680233
Number of successful extensions: 114089
Number of sequences better than 10.0: 645
Number of HSP's better than 10.0 without gapping: 80282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100719
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 62360592902
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
