>KMC002566A_C01 KMC002566A_c01
tttatttattggcctttttcgttatggggtagaattcaactttagagatGATTAATAAGC
GACAGGGGTGGCCATTAAAGTTTATCAACCTAGAAAGTTCAATCCATCAGACACATAACT
TACATCGCCATTAACAGATATAACATTCAGATTTTTTTTTTACTTTACAGAGTCCTCGTA
TGGTGAGGATTACCCACAACAAAAATAGTTTCTTTCCCAGCCTGATAATCTGAAAATGGC
TGTGCCCTTGTGCTCCACCACCAACCCTTGCTTTCACACGTGAATGAAACATAAAAACAG
ACCAGCAAACCCTTGAAAGTTGACTCAAAACAGACACATGTTGGGTAGGGACCCAAGTTT
ACATCACTAAAACAACTTCAAATATTGTTGAATACAATCTGTTGTGTGCTTTAAGTCTTG
CTAATTTGAAGCGGGGACAGGAGGCCGACCTCGTCCCATTGCAAAGCCCCGTGTTCCATC
TGGCATTTTGGGTCCTGGGGGCGGCTTCGATGCTTCGGCAGCATGAGTAGATGATGCACA
TCCATGGCCCATTCCATTTACGGCACGATATTTATGTTTTCTTGATCTTCCTTGATTTCT
ATACCGCTGCCCACCTTTATCCTTTGACAAATGATCCCCATCCTCTTCCTCAGGAGTATC
TTCATGATGCTCAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002566A_C01 KMC002566A_c01
         (674 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAB11080.1| contains similarity to RNA-binding protein~gene_...    92  8e-18
ref|NP_568660.1| RRM-containing protein; protein id: At5g46250.1...    92  8e-18
dbj|BAB90138.1| RNA-binding protein-like [Oryza sativa (japonica...    60  4e-08
ref|NP_188540.1| RNA-binding protein, putative; protein id: At3g...    49  6e-05
gb|AAL06505.1|AF412052_1 At2g43970/F6E13.10 [Arabidopsis thaliana]     49  8e-05

>dbj|BAB11080.1| contains similarity to RNA-binding protein~gene_id:MPL12.3
           [Arabidopsis thaliana]
          Length = 411

 Score = 91.7 bits (226), Expect = 8e-18
 Identities = 44/87 (50%), Positives = 56/87 (63%), Gaps = 6/87 (6%)
 Frame = -3

Query: 669 HHEDTPEEEDGDHLSKDKGGQRYRNQGRSRKHKYRAVNGMGHGCASST-----HAAEASK 505
           HH D P ++DG +  KDK G + R  G+ R+  ++  NG+GHG ASS+     H  E SK
Sbjct: 318 HHSDNPADDDGGNHQKDKNGNKGRVVGQGRRQNHQGGNGIGHGTASSSSHPNYHPVEVSK 377

Query: 504 PPPGPKMPDGTRGFAMGRGRP-PVPAS 427
            PPGP+MPDGTRGF MGRG+  P P S
Sbjct: 378 RPPGPRMPDGTRGFTMGRGKAIPPPTS 404

>ref|NP_568660.1| RRM-containing protein; protein id: At5g46250.1, supported by cDNA:
           gi_15215747 [Arabidopsis thaliana]
           gi|15215748|gb|AAK91419.1| AT5g46250/MPL12_3
           [Arabidopsis thaliana] gi|23308375|gb|AAN18157.1|
           At5g46250/MPL12_3 [Arabidopsis thaliana]
          Length = 422

 Score = 91.7 bits (226), Expect = 8e-18
 Identities = 44/87 (50%), Positives = 56/87 (63%), Gaps = 6/87 (6%)
 Frame = -3

Query: 669 HHEDTPEEEDGDHLSKDKGGQRYRNQGRSRKHKYRAVNGMGHGCASST-----HAAEASK 505
           HH D P ++DG +  KDK G + R  G+ R+  ++  NG+GHG ASS+     H  E SK
Sbjct: 329 HHSDNPADDDGGNHQKDKNGNKGRVVGQGRRQNHQGGNGIGHGTASSSSHPNYHPVEVSK 388

Query: 504 PPPGPKMPDGTRGFAMGRGRP-PVPAS 427
            PPGP+MPDGTRGF MGRG+  P P S
Sbjct: 389 RPPGPRMPDGTRGFTMGRGKAIPPPTS 415

>dbj|BAB90138.1| RNA-binding protein-like [Oryza sativa (japonica cultivar-group)]
          Length = 389

 Score = 59.7 bits (143), Expect = 4e-08
 Identities = 32/77 (41%), Positives = 47/77 (60%), Gaps = 1/77 (1%)
 Frame = -3

Query: 666 HEDTPEEEDGDHLSKDKGGQRYRNQGRSRKHKYRAVNGMGHGCASSTHAAE-ASKPPPGP 490
           HED  + +  +     KG  RY++QGR +  +    +G GH  + +T  ++  +KP PGP
Sbjct: 307 HEDMHQHQKVNAKGGRKG--RYKSQGRGQIQQN--TSGQGHVSSPATSGSDHVNKPIPGP 362

Query: 489 KMPDGTRGFAMGRGRPP 439
           +MPDGTRGF MGRG+ P
Sbjct: 363 RMPDGTRGFTMGRGKSP 379

>ref|NP_188540.1| RNA-binding protein, putative; protein id: At3g19090.1 [Arabidopsis
           thaliana] gi|11994567|dbj|BAB02607.1| RNA-binding
           protein-like [Arabidopsis thaliana]
          Length = 455

 Score = 48.9 bits (115), Expect = 6e-05
 Identities = 32/93 (34%), Positives = 44/93 (46%), Gaps = 12/93 (12%)
 Frame = -3

Query: 669 HHEDTPEEEDGDHLSKDKGGQRYRNQGRSRKHKYRAVNGMGHGCA---------SSTHAA 517
           H  ++  + DGD  +      + R +GR R  +  AV G G              S  + 
Sbjct: 359 HLTESQLDNDGDDNNVGGLWGKGRGKGRGRSPRSYAVGGGGRSFGIGLGVSLGIPSLGSH 418

Query: 516 EASKPPP---GPKMPDGTRGFAMGRGRPPVPAS 427
           E+S P     GP+MPDGTRGF MGRG+P +  S
Sbjct: 419 ESSSPKTATKGPRMPDGTRGFTMGRGKPSISLS 451

>gb|AAL06505.1|AF412052_1 At2g43970/F6E13.10 [Arabidopsis thaliana]
          Length = 329

 Score = 48.5 bits (114), Expect = 8e-05
 Identities = 31/97 (31%), Positives = 46/97 (46%), Gaps = 22/97 (22%)
 Frame = -3

Query: 666 HEDTPEEEDGDHLSKDKG---GQRYRNQGRSRKHKYRAV---NGMGH---------GCAS 532
           +E    +  G +  + KG   GQ ++NQ ++  H +      NG G+         G   
Sbjct: 227 NEKAAGQRKGRNRGRGKGRGRGQPHQNQNQNNNHSHNQNHNHNGRGNHHHHHHHQVGTQP 286

Query: 531 STHAAE-------ASKPPPGPKMPDGTRGFAMGRGRP 442
           S +            + PPGP+MPDGTRGF+MGRG+P
Sbjct: 287 SNNPMNNMEQPGMGKQQPPGPRMPDGTRGFSMGRGKP 323

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 630,080,153
Number of Sequences: 1393205
Number of extensions: 15001301
Number of successful extensions: 52247
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 45725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 51861
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29704274460
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF011a02_f BP028785 1 353 2 MF014c02_f BP028971 50 583 3 GENf080f10 BP061792 50 377 4 GNf058b04 BP071661 70 541 5 MR047a02_f BP079601 81 482 6 SPD010g06_f BP044823 85 694 7 SPDL065h04_f BP056072 104 328 8 SPD096a09_f BP051640 110 612 9 GNf015g04 BP068478 115 315 10 SPDL093g01_f BP057860 118 583 11 GNf046g05 BP070785 123 601 12 MR095d10_f BP083293 123 520 13 MPD040a05_f AV772702 162 627 14 MR082c08_f BP082298 163 489 15 MR083g07_f BP082415 163 490 16 GNf027h08 BP069351 163 555 17 MR075a04_f BP081734 163 564 18 MR064d03_f BP080907 163 249 19 MWM092b08_f AV766235 163 582 20 MR032g03_f BP078487 163 526

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