>KMC002508A_C01 KMC002508A_c01
agcatttagaatctctggtCCAAGTAACCACCGGTTTATTCAAGCAAGACTCTCAGTGGG
TGTTTATTAGTCAAACATAAACAAAACTGTGAAATTAAAATTTAAAAATCAAAGCACAAG
AAGACAAATGCTAAACTAGCTTCACTCATACTTTTGGCGCCTTGCTCAAAAAATCCTTAA
GCACATCCATAACCGCTCCAAAATCCGCTTTCACAGTAACAGGTGGGTTAGAAAACCGAC
CCAAACCCGGAATAGCCTTAGAGTGATAATCAATCTGGCTCTGTGTAAACTTCAACCCAT
GCGCGGTGCTCACCACCACCGCCCTATCACCCGCCGCAATCACCCCGGAGTTTCTCAGCT
TCGTCAGTGCCGTCAAAGCCACCCCAGTATGCGGGCAAATAAACATCCCAGTCGAATCCG
CCAACGCCATCGCATCCATCAGCTCCTCCTCCGTCGCCTCCTCCACAATCCCACCATAAC
TCTTCAGCGCATGCACCGGCCTGTCAATCGAAACCGGGTCACCAATCTGAATCGCCGAAG
CGAA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002508A_C01 KMC002508A_c01
         (544 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAB04607.1| threonine synthase                                     203  1e-51
ref|NP_194713.1| threonine synthase; protein id: At4g29840.1 [Ar...   203  1e-51
sp|Q9MT28|THRC_SOLTU Threonine synthase, chloroplast precursor (...   196  2e-49
ref|NP_565047.1| threonine synthase, putative; protein id: At1g7...   194  4e-49
pdb|1E5X|A Chain A, Structure Of Threonine Synthase From Arabido...   193  1e-48

>gb|AAB04607.1| threonine synthase
          Length = 525

 Score =  203 bits (517), Expect = 1e-51
 Identities = 107/127 (84%), Positives = 111/127 (87%), Gaps = 1/127 (0%)
 Frame = -1

Query: 544 FASAIQIGDPVSIDRPVHALKSYGGIVEEATEEELMDAMALADSTGMFICPHTGVALTAL 365
           FASAIQIGDPVSIDR V+ALK   GIVEEATEEELMDAMA ADSTGMFICPHTGVALTAL
Sbjct: 392 FASAIQIGDPVSIDRAVYALKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL 451

Query: 364 TKLRNSGVIAAGDRAVVVSTAHGLKFTQSQIDYHSKAIPGLG-RFSNPPVTVKADFGAVM 188
            KLRN GVIA  DR VVVSTAHGLKFTQS+IDYHS AIP +  RFSNPPV VKADFGAVM
Sbjct: 452 FKLRNQGVIAPTDRTVVVSTAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVM 511

Query: 187 DVLKDFL 167
           DVLK +L
Sbjct: 512 DVLKSYL 518

>ref|NP_194713.1| threonine synthase; protein id: At4g29840.1 [Arabidopsis thaliana]
           gi|20140904|sp|Q9S7B5|THRC_ARATH Threonine synthase,
           chloroplast precursor (TS) gi|7488373|pir||T08545
           threonine synthase (EC 4.2.3.1) precursor - Arabidopsis
           thaliana gi|4850369|dbj|BAA77707.1| threonine synthase
           [Arabidopsis thaliana] gi|4914408|emb|CAB43659.1|
           threonine synthase [Arabidopsis thaliana]
           gi|7269883|emb|CAB79742.1| threonine synthase
           [Arabidopsis thaliana]
          Length = 526

 Score =  203 bits (517), Expect = 1e-51
 Identities = 107/127 (84%), Positives = 111/127 (87%), Gaps = 1/127 (0%)
 Frame = -1

Query: 544 FASAIQIGDPVSIDRPVHALKSYGGIVEEATEEELMDAMALADSTGMFICPHTGVALTAL 365
           FASAIQIGDPVSIDR V+ALK   GIVEEATEEELMDAMA ADSTGMFICPHTGVALTAL
Sbjct: 393 FASAIQIGDPVSIDRAVYALKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL 452

Query: 364 TKLRNSGVIAAGDRAVVVSTAHGLKFTQSQIDYHSKAIPGLG-RFSNPPVTVKADFGAVM 188
            KLRN GVIA  DR VVVSTAHGLKFTQS+IDYHS AIP +  RFSNPPV VKADFGAVM
Sbjct: 453 FKLRNQGVIAPTDRTVVVSTAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVM 512

Query: 187 DVLKDFL 167
           DVLK +L
Sbjct: 513 DVLKSYL 519

>sp|Q9MT28|THRC_SOLTU Threonine synthase, chloroplast precursor (TS)
           gi|8439547|gb|AAF74984.1|AF082894_1 threonine synthase
           [Solanum tuberosum]
          Length = 519

 Score =  196 bits (498), Expect = 2e-49
 Identities = 104/127 (81%), Positives = 110/127 (85%), Gaps = 1/127 (0%)
 Frame = -1

Query: 544 FASAIQIGDPVSIDRPVHALKSYGGIVEEATEEELMDAMALADSTGMFICPHTGVALTAL 365
           FASAIQIGDPVSIDR V AL+   GIVEEATEEELMDAMA ADSTGMFICPHTGVALTAL
Sbjct: 386 FASAIQIGDPVSIDRAVFALQQCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL 445

Query: 364 TKLRNSGVIAAGDRAVVVSTAHGLKFTQSQIDYHSKAIPGLG-RFSNPPVTVKADFGAVM 188
            KLRNSGVIA  DR VVVSTAHGLKFTQS+IDYHSK I  +  RF+NPPV VKADFG+VM
Sbjct: 446 FKLRNSGVIAPTDRTVVVSTAHGLKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVM 505

Query: 187 DVLKDFL 167
           DVLK +L
Sbjct: 506 DVLKSYL 512

>ref|NP_565047.1| threonine synthase, putative; protein id: At1g72810.1, supported by
           cDNA: gi_20466325 [Arabidopsis thaliana]
           gi|25406213|pir||A96753 probable threonine synthase
           [imported] - Arabidopsis thaliana
           gi|5903070|gb|AAD55628.1|AC008017_1 Putative threonine
           synthase [Arabidopsis thaliana]
           gi|20466326|gb|AAM20480.1| threonine synthase, putative
           [Arabidopsis thaliana] gi|25084052|gb|AAN72162.1|
           threonine synthase, putative [Arabidopsis thaliana]
          Length = 516

 Score =  194 bits (494), Expect = 4e-49
 Identities = 103/132 (78%), Positives = 110/132 (83%), Gaps = 1/132 (0%)
 Frame = -1

Query: 544 FASAIQIGDPVSIDRPVHALKSYGGIVEEATEEELMDAMALADSTGMFICPHTGVALTAL 365
           FASAIQIGDPVSIDR V+ALK   GIVEEATEEELMDA ALADSTGMFICPHTGVALTAL
Sbjct: 385 FASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALADSTGMFICPHTGVALTAL 444

Query: 364 TKLRNSGVIAAGDRAVVVSTAHGLKFTQSQIDYHSKAIPGLG-RFSNPPVTVKADFGAVM 188
            KLR SGVI A DR VVVSTAHGLKFTQS+IDYHSK I  +  R +NPPV VKA FG+VM
Sbjct: 445 MKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVM 504

Query: 187 DVLKDFLSKAPK 152
           DVLK++L    K
Sbjct: 505 DVLKEYLKSNDK 516

>pdb|1E5X|A Chain A, Structure Of Threonine Synthase From Arabidopsis Thaliana
           gi|15825883|pdb|1E5X|B Chain B, Structure Of Threonine
           Synthase From Arabidopsis Thaliana
          Length = 486

 Score =  193 bits (490), Expect = 1e-48
 Identities = 103/127 (81%), Positives = 106/127 (83%), Gaps = 1/127 (0%)
 Frame = -1

Query: 544 FASAIQIGDPVSIDRPVHALKSYGGIVEEATEEELMDAMALADSTGMFICPHTGVALTAL 365
           FASAIQIGDPVSIDR V+ALK   GIVEEATEEEL DA A ADSTG FICPHTGVALTAL
Sbjct: 353 FASAIQIGDPVSIDRAVYALKKCNGIVEEATEEELXDAXAQADSTGXFICPHTGVALTAL 412

Query: 364 TKLRNSGVIAAGDRAVVVSTAHGLKFTQSQIDYHSKAIPGLG-RFSNPPVTVKADFGAVM 188
            KLRN GVIA  DR VVVSTAHGLKFTQS+IDYHS AIP    RFSNPPV VKADFGAV 
Sbjct: 413 FKLRNQGVIAPTDRTVVVSTAHGLKFTQSKIDYHSNAIPDXACRFSNPPVDVKADFGAVX 472

Query: 187 DVLKDFL 167
           DVLK +L
Sbjct: 473 DVLKSYL 479

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 512,925,066
Number of Sequences: 1393205
Number of extensions: 13118670
Number of successful extensions: 195857
Number of sequences better than 10.0: 4453
Number of HSP's better than 10.0 without gapping: 75852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136603
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18750593680
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENf080b05 BP061771 1 363 2 GENf075d07 BP061566 20 263 3 MF041a10_f BP030418 20 515 4 GENf074f10 BP061533 20 470 5 MR035g06_f BP078732 20 383 6 MPDL039g05_f AV778489 31 553 7 GNf046d08 BP070758 38 562 8 MWL008b06_f AV768702 48 193

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