>KMC002464A_C01 KMC002464A_c01
aagcgacaaaaaaaaaactatgccagtaagtctgGACAAGAAAATACTAAATAAAATGTT
TGGCCAACAAGTCAAAGTCAGCCCTGTCCATTATAGGCATACATCAAATTAATGAATTTT
CAAGGCAAGATGAACTAGGCAGAAAATGAAATCAAGCTTATACGCATTCTAGAATATATC
ATTCTGGATTAACTTCCACTAGCTTGGCATTGATGAAATAAGATGCAGCAATGGATTTTT
TTTTTCTTTCCCTTCAAGTGAAATAAGTTATGAAAACAACCTGGTAGCAGATAGTTCCAA
TTGCATACATTTTCTACTTGAAGAATTCCATAACCCAGAAGGAACGAACATAGCATTCAT
ATCTAATGCCACTAAATCCAGCCGCAGTTGCTAGCTCCATGAATTCTTGCTCAGTTCGCT
CTTTCCCTCCTGGGTTTTGAGTCATCATCATTACATCGATTTGGGAGACAGACTTCCAAG
TAGGATTATTCTCAGGTACAATGGAAAGAACTGCCTCAACAACAATCACCTTTCCATCAT
CAGGAACAGCAGCATAGCAGTTCTTCAAAAGCTTCAAGCAGTGTTCATCACTCCAATCAT
GAAGTATCCACTTCAAAAAGATGGCATCTCCCTTAGGCACCC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002464A_C01 KMC002464A_c01
         (642 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||JQ2344 catechol O-methyltransferase (EC 2.1.1.6) III - comm...   152  4e-36
gb|AAL91506.1|AF484252_1 caffeic acid O-methyltransferase II [Ni...   152  4e-36
emb|CAD29457.1| caffeic acid O-methyltransferase [Rosa chinensis]     147  2e-34
sp|Q43609|COMT_PRUDU Caffeic acid 3-O-methyltransferase (S-adeno...   145  3e-34
prf||2119166A caffeic acid O-methyltransferase                        145  4e-34

>pir||JQ2344 catechol O-methyltransferase (EC 2.1.1.6) III - common tobacco
           gi|429114|emb|CAA50561.1| catechol O-methyltransferase
           [Nicotiana tabacum]
          Length = 365

 Score =  152 bits (384), Expect = 4e-36
 Identities = 71/108 (65%), Positives = 85/108 (77%)
 Frame = -3

Query: 640 VPKGDAIFLKWILHDWSDEHCLKLLKNCYAAVPDDGKVIVVEAVLSIVPENNPTWKSVSQ 461
           VP+GDAIF+KWILHDWSD H LKLLKNCY A+PD+GKVIVVEA+L + P+ +     VSQ
Sbjct: 258 VPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPDNGKVIVVEAILPVKPDIDTAVVGVSQ 317

Query: 460 IDVMMMTQNPGGKERTEQEFMELATAAGFSGIRYECYVRSFWVMEFFK 317
            D++MM QNPGGKER+E+EF  LAT AGF G+   C V +FWVMEF K
Sbjct: 318 CDLIMMAQNPGGKERSEEEFRALATEAGFKGVNLICCVCNFWVMEFCK 365

>gb|AAL91506.1|AF484252_1 caffeic acid O-methyltransferase II [Nicotiana tabacum]
          Length = 364

 Score =  152 bits (384), Expect = 4e-36
 Identities = 71/108 (65%), Positives = 85/108 (77%)
 Frame = -3

Query: 640 VPKGDAIFLKWILHDWSDEHCLKLLKNCYAAVPDDGKVIVVEAVLSIVPENNPTWKSVSQ 461
           VP+GDAIF+KWILHDWSD H LKLLKNCY A+PD+GKVIVVEA+L + P+ +     VSQ
Sbjct: 257 VPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPDNGKVIVVEAILPVKPDIDTAVVGVSQ 316

Query: 460 IDVMMMTQNPGGKERTEQEFMELATAAGFSGIRYECYVRSFWVMEFFK 317
            D++MM QNPGGKER+E+EF  LAT AGF G+   C V +FWVMEF K
Sbjct: 317 CDLIMMAQNPGGKERSEEEFRALATEAGFKGVNLICCVCNFWVMEFCK 364

>emb|CAD29457.1| caffeic acid O-methyltransferase [Rosa chinensis]
          Length = 365

 Score =  147 bits (370), Expect = 2e-34
 Identities = 67/108 (62%), Positives = 81/108 (74%)
 Frame = -3

Query: 640 VPKGDAIFLKWILHDWSDEHCLKLLKNCYAAVPDDGKVIVVEAVLSIVPENNPTWKSVSQ 461
           VPKGDAIF+KWI HDWSDEHCLK LKNCYAA+PD+GKVI+ E +L + P+ +   K V  
Sbjct: 256 VPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDTSLATKGVVH 315

Query: 460 IDVMMMTQNPGGKERTEQEFMELATAAGFSGIRYECYVRSFWVMEFFK 317
           IDV+M+  NPGGKERTEQEF  LA  +GF GIR  C   + +V+EF K
Sbjct: 316 IDVVMLAHNPGGKERTEQEFEALAKGSGFQGIRVACNAFNTYVIEFLK 363

>sp|Q43609|COMT_PRUDU Caffeic acid 3-O-methyltransferase
           (S-adenosysl-L-methionine:caffeic acid
           3-O-methyltransferase) (COMT) gi|602588|emb|CAA58218.1|
           caffeic O-methyltransferase [Prunus dulcis]
          Length = 365

 Score =  145 bits (367), Expect = 3e-34
 Identities = 66/108 (61%), Positives = 80/108 (73%)
 Frame = -3

Query: 640 VPKGDAIFLKWILHDWSDEHCLKLLKNCYAAVPDDGKVIVVEAVLSIVPENNPTWKSVSQ 461
           VPKGDAIF+KWI HDWSDEHCLK LKNCYAA+PD+GKVI+ E +L + P+++   K V  
Sbjct: 256 VPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDSSLATKGVVH 315

Query: 460 IDVMMMTQNPGGKERTEQEFMELATAAGFSGIRYECYVRSFWVMEFFK 317
           IDV+M+  NPGGKERTEQEF  LA  AGF G    C   + +V+EF K
Sbjct: 316 IDVIMLAHNPGGKERTEQEFQALAKGAGFQGFNVACSAFNTYVIEFLK 363

>prf||2119166A caffeic acid O-methyltransferase
          Length = 365

 Score =  145 bits (366), Expect = 4e-34
 Identities = 65/108 (60%), Positives = 80/108 (73%)
 Frame = -3

Query: 640 VPKGDAIFLKWILHDWSDEHCLKLLKNCYAAVPDDGKVIVVEAVLSIVPENNPTWKSVSQ 461
           VPK DA+F+KWI HDWSDEHC+K LKNCY AVPD+GKVIV E +L + P+++   K V  
Sbjct: 256 VPKADAVFMKWICHDWSDEHCIKFLKNCYDAVPDNGKVIVAECILPVAPDSSLATKGVVH 315

Query: 460 IDVMMMTQNPGGKERTEQEFMELATAAGFSGIRYECYVRSFWVMEFFK 317
           IDV+M+  NPGGKERTE+EF  LA AAGF G R  C   + ++MEF K
Sbjct: 316 IDVIMLAHNPGGKERTEKEFEALAKAAGFQGFRVVCCAFNTYIMEFLK 363

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 529,802,208
Number of Sequences: 1393205
Number of extensions: 11055119
Number of successful extensions: 37961
Number of sequences better than 10.0: 246
Number of HSP's better than 10.0 without gapping: 30834
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36171
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27007650415
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENf069b07 BP061306 1 364 2 MR056e10_f BP080316 35 429 3 MR002a04_f BP076045 35 418 4 GNf084h02 BP073595 36 574 5 MWL001e08_f AV768596 42 313 6 SPD003f05_f BP044252 215 353 7 GNf030g02 BP069567 247 643

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