>KMC002373A_C01 KMC002373A_c01
attaacaaagaggatgggtgttgTTGGCATATTAAACCAGCCGGTCTCTTAACTTAAAAT
ACAATATTACTTTCGATGCTCTTTAGGCAAAGGACCCATTACATATTTGTAAATAAATGT
GTATCACTATGATTTAATATGAAGTACATATATGACCTTGATCAATTGAAATTTAGAAGT
CGCGGTTGCTCGCTCATTTCAAAGAAAGAATCACTATAGAAGAAGCTTATACAATTTTCA
TCTATTCACTAACTATAATCATTGTTCATGCCTCGAACCTTATTCTTTTAAATGAATGCA
TTCTGTATTAAACCGTATCTTACTTCACCTTAGCTGGACTAGAAGTGACAGATTTCTTTA
TACAGTCTACTATTCCTTCACAATCTGCTTCAAGAGGACACACTGTGTCTTCATCATCCT
CTGCATATGAATGAGGTGACCGGAGGTTCCCATGGGTTTGACAAGCCTTCATTGTCTTAC
AATGTTCAGCTTCGTCATCTCGGACATTTACAAATACGTCGTACAGATTCTCTATCTTAG
GCCTTCTAGTATTTGGAACTCTGGAAGTTTGAAACTCATCAAATAAATA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002373A_C01 KMC002373A_c01
         (589 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_567658.1| putative protein; protein id: At4g22260.1, supp...   139  3e-32
gb|AAG02286.1|AF177979_1 plastid terminal oxidase [Lycopersicon ...   138  5e-32
emb|CAA06190.1| Immutans protein [Arabidopsis thaliana]               138  6e-32
gb|AAC35554.3| oxidase [Oryza sativa (japonica cultivar-group)]       136  2e-31
gb|AAK70807.1| oxidase [Oryza sativa]                                 136  2e-31

>ref|NP_567658.1| putative protein; protein id: At4g22260.1, supported by cDNA:
           gi_11908101, supported by cDNA: gi_12642913, supported
           by cDNA: gi_13265406, supported by cDNA: gi_15010795,
           supported by cDNA: gi_4138854 [Arabidopsis thaliana]
           gi|25453513|pir||T52422 alternative oxidase-related
           protein IMMUTANS [validated] - Arabidopsis thaliana
           gi|4138855|gb|AAD03599.1| IMMUTANS [Arabidopsis
           thaliana] gi|11692822|gb|AAG40014.1|AF324663_1 AT4g22260
           [Arabidopsis thaliana]
           gi|11908102|gb|AAG41480.1|AF326898_1 unknown protein
           [Arabidopsis thaliana]
           gi|12642914|gb|AAK00399.1|AF339717_1 unknown protein
           [Arabidopsis thaliana] gi|15010796|gb|AAK74057.1|
           AT4g22260/T10I14_90 [Arabidopsis thaliana]
           gi|23308315|gb|AAN18127.1| At4g22260/T10I14_90
           [Arabidopsis thaliana]
          Length = 351

 Score =  139 bits (350), Expect = 3e-32
 Identities = 67/88 (76%), Positives = 73/88 (82%), Gaps = 5/88 (5%)
 Frame = -1

Query: 589 YLFDEFQTSRVPNTRRPKIENLYDVFVNVRDDEAEHCKTMKACQTHGNLRSPHSYAEDDE 410
           YLFDEFQTSR PNTRRP IENLYDVFVN+RDDEAEHCKTM+ACQT G+LRSPHS  EDD+
Sbjct: 264 YLFDEFQTSRTPNTRRPVIENLYDVFVNIRDDEAEHCKTMRACQTLGSLRSPHSILEDDD 323

Query: 409 -----DTVCPLEADCEGIVDCIKKSVTS 341
                  V P EA CEGIVDC+KKS+TS
Sbjct: 324 TEEESGCVVPEEAHCEGIVDCLKKSITS 351

>gb|AAG02286.1|AF177979_1 plastid terminal oxidase [Lycopersicon esculentum]
           gi|9937101|gb|AAG02287.1|AF177980_1 plastid terminal
           oxidase [Lycopersicon esculentum]
           gi|10505366|gb|AAG18449.1|AF302931_1 plastid quinol
           oxidase [Lycopersicon esculentum]
           gi|10505368|gb|AAG18450.1|AF302932_1 plastid quinol
           oxidase [Lycopersicon esculentum]
          Length = 366

 Score =  138 bits (348), Expect = 5e-32
 Identities = 67/87 (77%), Positives = 76/87 (87%), Gaps = 3/87 (3%)
 Frame = -1

Query: 589 YLFDEFQTSRVPNTRRPKIENLYDVFVNVRDDEAEHCKTMKACQTHGNLRSPHSYAEDD- 413
           YLFDEFQTSR PNTRRPKI+NLYDVF+N+RDDEAEHCKTMKACQTHG+LRSPH+   DD 
Sbjct: 275 YLFDEFQTSREPNTRRPKIDNLYDVFMNIRDDEAEHCKTMKACQTHGSLRSPHTDPCDDS 334

Query: 412 -EDTVCPL-EADCEGIVDCIKKSVTSS 338
            +DT C + +ADC GIVDCIKKSVT +
Sbjct: 335 EDDTGCSVPQADCIGIVDCIKKSVTDT 361

>emb|CAA06190.1| Immutans protein [Arabidopsis thaliana]
          Length = 351

 Score =  138 bits (347), Expect = 6e-32
 Identities = 66/88 (75%), Positives = 73/88 (82%), Gaps = 5/88 (5%)
 Frame = -1

Query: 589 YLFDEFQTSRVPNTRRPKIENLYDVFVNVRDDEAEHCKTMKACQTHGNLRSPHSYAEDDE 410
           YLFDEFQTSR PNTRRP IENLYDVFVN+RDDEAEHCKTM+ACQT G+LRSPHS  +DD+
Sbjct: 264 YLFDEFQTSRTPNTRRPVIENLYDVFVNIRDDEAEHCKTMRACQTLGSLRSPHSILDDDD 323

Query: 409 -----DTVCPLEADCEGIVDCIKKSVTS 341
                  V P EA CEGIVDC+KKS+TS
Sbjct: 324 TEEESGCVVPEEAHCEGIVDCLKKSITS 351

>gb|AAC35554.3| oxidase [Oryza sativa (japonica cultivar-group)]
          Length = 336

 Score =  136 bits (342), Expect = 2e-31
 Identities = 66/86 (76%), Positives = 73/86 (84%), Gaps = 3/86 (3%)
 Frame = -1

Query: 589 YLFDEFQTSRVPNTRRPKIENLYDVFVNVRDDEAEHCKTMKACQTHGNLRSPHSY---AE 419
           YLFDEFQT+RVP +RRPKI+NLYDVFVN+RDDEAEHCKTMKACQTHGNLRSPHS     E
Sbjct: 250 YLFDEFQTARVPCSRRPKIDNLYDVFVNIRDDEAEHCKTMKACQTHGNLRSPHSMQKCLE 309

Query: 418 DDEDTVCPLEADCEGIVDCIKKSVTS 341
            D + V P E DCEGIVDC+KKS+ S
Sbjct: 310 TDTECVIP-EDDCEGIVDCVKKSLVS 334

>gb|AAK70807.1| oxidase [Oryza sativa]
          Length = 158

 Score =  136 bits (342), Expect = 2e-31
 Identities = 66/86 (76%), Positives = 73/86 (84%), Gaps = 3/86 (3%)
 Frame = -1

Query: 589 YLFDEFQTSRVPNTRRPKIENLYDVFVNVRDDEAEHCKTMKACQTHGNLRSPHSY---AE 419
           YLFDEFQT+RVP +RRPKI+NLYDVFVN+RDDEAEHCKTMKACQTHGNLRSPHS     E
Sbjct: 72  YLFDEFQTARVPCSRRPKIDNLYDVFVNIRDDEAEHCKTMKACQTHGNLRSPHSMQKCLE 131

Query: 418 DDEDTVCPLEADCEGIVDCIKKSVTS 341
            D + V P E DCEGIVDC+KKS+ S
Sbjct: 132 TDTECVIP-EDDCEGIVDCVKKSLVS 156

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 452,401,338
Number of Sequences: 1393205
Number of extensions: 8914028
Number of successful extensions: 16805
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 16386
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16795
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22283372436
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf015h06 BP068489 1 459 2 MFB041e09_f BP036995 18 472 3 MR054f05_f BP080180 51 434 4 MF039b11_f BP030325 51 554 5 MFL003f06_f BP033579 54 592 6 GENf060b08 BP060887 169 542

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