Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002363A_C01 KMC002363A_c01
(707 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T12041 cysteine proteinase (EC 3.4.22.-) 3 precursor - kidn... 362 2e-99
pir||S24602 cysteine proteinase tpp (EC 3.4.22.-) - garden pea g... 343 1e-93
gb|AAL60580.1|AF454958_1 senescence-associated cysteine protease... 331 5e-90
pir||T06416 cysteine proteinase (EC 3.4.22.-) precursor - tomato... 329 3e-89
emb|CAB53515.1| cysteine protease [Solanum tuberosum] 327 1e-88
>pir||T12041 cysteine proteinase (EC 3.4.22.-) 3 precursor - kidney bean
gi|2511693|emb|CAB17076.1| cysteine proteinase precursor
[Phaseolus vulgaris]
Length = 455
Score = 362 bits (930), Expect = 2e-99
Identities = 173/228 (75%), Positives = 200/228 (86%), Gaps = 2/228 (0%)
Frame = +2
Query: 29 VLMFTLLAVSSAMDMSIISYDNSHMGNS--RTDDEVKNMYEEWLVKHGKVYNALGEKEKR 202
+L+F L A+SSA+DMSIISYDN+H + RTD+EV ++YEEWLVKHGK+YNALGEK+KR
Sbjct: 1 MLLFALFALSSALDMSIISYDNAHQDKATWRTDEEVNSLYEEWLVKHGKLYNALGEKDKR 60
Query: 203 FEIFKDNLKFIDEHNNADLNRSYKLGLNRFADLTNEEYRSKYFGTRVDPNRRMAKLRTKS 382
F+IFKDNL+FID+ N NR+YKLGLNRFADLTNEEYR++Y GT++DPNRR+ RT S
Sbjct: 61 FQIFKDNLRFIDQQNAE--NRTYKLGLNRFADLTNEEYRARYLGTKIDPNRRLG--RTPS 116
Query: 383 DRYAPRVGDKLPESVDWRKEGALVGVKDQGSCGSCWAFSAVTAVESINKIVTGDLVSLSV 562
+RYAPRVG+ LP+SVDWRKEGA+V VKDQ SCGSCWAFSA+ AVE INKIVTGDL+SLS
Sbjct: 117 NRYAPRVGETLPDSVDWRKEGAVVPVKDQASCGSCWAFSAIGAVEGINKIVTGDLISLSE 176
Query: 563 QELVDCDRSYNEGCNGGLMDYAFDFIINNGGIDSEEDYPYKGVDGRCD 706
QELVDCD YN GCNGGLMDYAF+FII NGGIDSEEDYPYKGVDGRCD
Sbjct: 177 QELVDCDTGYNMGCNGGLMDYAFEFIIKNGGIDSEEDYPYKGVDGRCD 224
>pir||S24602 cysteine proteinase tpp (EC 3.4.22.-) - garden pea
gi|3980198|emb|CAA46863.1| thiolprotease [Pisum sativum]
Length = 464
Score = 343 bits (880), Expect = 1e-93
Identities = 166/229 (72%), Positives = 193/229 (83%), Gaps = 2/229 (0%)
Frame = +2
Query: 26 IVLMFTLLAVSSAMDMSIISYDNSHMGNS--RTDDEVKNMYEEWLVKHGKVYNALGEKEK 199
I L FTL S A+DM IISYD +H S RT+D+V MYEEWLVKHGK YNALGEKEK
Sbjct: 10 ITLTFTL---SLALDMCIISYDKTHPDKSTPRTNDQVLTMYEEWLVKHGKNYNALGEKEK 66
Query: 200 RFEIFKDNLKFIDEHNNADLNRSYKLGLNRFADLTNEEYRSKYFGTRVDPNRRMAKLRTK 379
RFEIFKDNL FIDEHN+ +L S++LGLNRFADLTNEEYR+++ GTR++PNRR K+ ++
Sbjct: 67 RFEIFKDNLGFIDEHNSKNL--SFRLGLNRFADLTNEEYRTRFLGTRINPNRRNRKVNSQ 124
Query: 380 SDRYAPRVGDKLPESVDWRKEGALVGVKDQGSCGSCWAFSAVTAVESINKIVTGDLVSLS 559
++RYA RVGDKLPESVDWRKEGA+VGVKDQGSCGSCWAFSA+ AVE +NK+ TGDL+SLS
Sbjct: 125 TNRYATRVGDKLPESVDWRKEGAVVGVKDQGSCGSCWAFSAIAAVEGVNKLATGDLISLS 184
Query: 560 VQELVDCDRSYNEGCNGGLMDYAFDFIINNGGIDSEEDYPYKGVDGRCD 706
QELVDCD SYNEGCNGGLMDYAF+FIIN + EEDYPY+ +DGRCD
Sbjct: 185 EQELVDCDTSYNEGCNGGLMDYAFEFIINMVALTPEEDYPYRAIDGRCD 233
>gb|AAL60580.1|AF454958_1 senescence-associated cysteine protease [Brassica oleracea]
Length = 485
Score = 331 bits (849), Expect = 5e-90
Identities = 162/228 (71%), Positives = 186/228 (81%), Gaps = 1/228 (0%)
Frame = +2
Query: 26 IVLMFTLLAVSSAMDMSIISYD-NSHMGNSRTDDEVKNMYEEWLVKHGKVYNALGEKEKR 202
++L T++ VSSAMDMSIISYD N H +SR+D EV +YEEWLVKHGK N+L EK++R
Sbjct: 9 VILFLTMIVVSSAMDMSIISYDKNHHTVSSRSDAEVSRLYEEWLVKHGKAQNSLTEKDRR 68
Query: 203 FEIFKDNLKFIDEHNNADLNRSYKLGLNRFADLTNEEYRSKYFGTRVDPNRRMAKLRTKS 382
FEIFKDNL+FIDEHN +L SY+LGL +FADLTN+EYRS Y G+R+ K S
Sbjct: 69 FEIFKDNLRFIDEHNGKNL--SYRLGLTKFADLTNDEYRSMYLGSRLK-----RKATKSS 121
Query: 383 DRYAPRVGDKLPESVDWRKEGALVGVKDQGSCGSCWAFSAVTAVESINKIVTGDLVSLSV 562
RY RVGD +PESVDWRKEGA+ VKDQGSCGSCWAFS + AVE INKIVTGDL++LS
Sbjct: 122 LRYEVRVGDAIPESVDWRKEGAVAEVKDQGSCGSCWAFSTIGAVEGINKIVTGDLITLSE 181
Query: 563 QELVDCDRSYNEGCNGGLMDYAFDFIINNGGIDSEEDYPYKGVDGRCD 706
QELVDCD SYNEGCNGGLMDYAF+FIINNGGID+EEDYPYKGVDGRCD
Sbjct: 182 QELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGVDGRCD 229
>pir||T06416 cysteine proteinase (EC 3.4.22.-) precursor - tomato
gi|2828252|emb|CAA05894.1| CYP1 [Lycopersicon
esculentum] gi|5726641|gb|AAD48496.1|AF172856_1 cysteine
protease TDI-65 [Lycopersicon esculentum]
Length = 466
Score = 329 bits (843), Expect = 3e-89
Identities = 158/228 (69%), Positives = 190/228 (83%)
Frame = +2
Query: 23 AIVLMFTLLAVSSAMDMSIISYDNSHMGNSRTDDEVKNMYEEWLVKHGKVYNALGEKEKR 202
+I+LM +SSA DMSIISYD +H+ + RTDDEV +YE WL++HGK YNALGEK+KR
Sbjct: 11 SILLMLIFSTLSSASDMSIISYDETHI-HRRTDDEVSALYESWLIEHGKSYNALGEKDKR 69
Query: 203 FEIFKDNLKFIDEHNNADLNRSYKLGLNRFADLTNEEYRSKYFGTRVDPNRRMAKLRTKS 382
F+IFKDNL++IDE N+ N+SYKLGL +FADLTNEEYRS Y GT+ +R+ + KS
Sbjct: 70 FQIFKDNLRYIDEQNSVP-NQSYKLGLTKFADLTNEEYRSIYLGTKSSGDRKKLS-KNKS 127
Query: 383 DRYAPRVGDKLPESVDWRKEGALVGVKDQGSCGSCWAFSAVTAVESINKIVTGDLVSLSV 562
DRY P+VGD LPES+DWR++G LVGVKDQGSCGSCWAFSAV A+ESIN IVTG+L+SLS
Sbjct: 128 DRYLPKVGDSLPESIDWREKGVLVGVKDQGSCGSCWAFSAVAAMESINAIVTGNLISLSE 187
Query: 563 QELVDCDRSYNEGCNGGLMDYAFDFIINNGGIDSEEDYPYKGVDGRCD 706
QELVDCDRSYNEGC+GGLMDYAF+F+I NGGID+EEDYPYK +G CD
Sbjct: 188 QELVDCDRSYNEGCDGGLMDYAFEFVIKNGGIDTEEDYPYKERNGVCD 235
>emb|CAB53515.1| cysteine protease [Solanum tuberosum]
Length = 466
Score = 327 bits (838), Expect = 1e-88
Identities = 158/228 (69%), Positives = 189/228 (82%)
Frame = +2
Query: 23 AIVLMFTLLAVSSAMDMSIISYDNSHMGNSRTDDEVKNMYEEWLVKHGKVYNALGEKEKR 202
+++LM +SSA DMSIISYD +H+ + R+DDEV +YE WL++HGK YNALGEK+KR
Sbjct: 11 SLLLMLIFSTLSSASDMSIISYDETHI-HHRSDDEVSALYESWLIEHGKSYNALGEKDKR 69
Query: 203 FEIFKDNLKFIDEHNNADLNRSYKLGLNRFADLTNEEYRSKYFGTRVDPNRRMAKLRTKS 382
F+IFKDNLK+IDE N+ N+SYKLGL +FADLTNEEYRS Y GT+ +RR + KS
Sbjct: 70 FQIFKDNLKYIDEQNSVP-NQSYKLGLTKFADLTNEEYRSIYLGTKSSGDRRKLS-KNKS 127
Query: 383 DRYAPRVGDKLPESVDWRKEGALVGVKDQGSCGSCWAFSAVTAVESINKIVTGDLVSLSV 562
DRY P+VGD LPESVDWR +G LVGVKDQGSCGSCWAFSAV A+ESIN IVTG+L+SLS
Sbjct: 128 DRYLPKVGDSLPESVDWRDKGVLVGVKDQGSCGSCWAFSAVAAMESINAIVTGNLISLSE 187
Query: 563 QELVDCDRSYNEGCNGGLMDYAFDFIINNGGIDSEEDYPYKGVDGRCD 706
QELVDCD+SYNEGC+GGLMDYAF+F+INNGGID+EEDYPYK + CD
Sbjct: 188 QELVDCDKSYNEGCDGGLMDYAFEFVINNGGIDTEEDYPYKERNDVCD 235
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 643,392,497
Number of Sequences: 1393205
Number of extensions: 14876314
Number of successful extensions: 62999
Number of sequences better than 10.0: 1123
Number of HSP's better than 10.0 without gapping: 55848
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 60800
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32373034405
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
