KMC002361A_c02
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002361A_C02 KMC002361A_c02
attctttattagatttaaatttttCGAAAATAATCAATTCTGATTTTATTTTCAAATTTT
ATTTTCTAATATCTAGTTATAGATAAACACAGCTCTAGAATGAAATAGAAGTCACAACAT
TTGCTAACAAGATCTCGCTCAATAATTCTCAGCTAATTCTAACTTGCATCTCAAGTGCAC
CAAGTGGAGGTGGTCAACAACAAAAGAAGTAACCAGTGGCGTCCTTAGCTAACACACGGC
ATAATCATTCTTCAGTATATTCCCGGAATGATTCTGTAAGGAACCTTGTCGCAATACAGT
TTCCAGTACTTGCCATACTTGGATCTGCATCGATCGTCATCCCTTTTTGCTCGATCAAAA
AGAAGGATGGTAAGAAATATCACATAGAAGTAAGGTAAAAAATGATTGAAAAGAGCTGGC
ACTGTCCAGAAGAAAGCAGCTAGTATTTCAGGGACATAATGAAAATGACGAGATAATCCC
CACCATCCAGAGGTTAAAAGAAGGCTTCTTTTTGTTTGCCC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002361A_C02 KMC002361A_c02
         (521 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_175460.1| sterol delta7 reductase; protein id: At1g50430....   179  2e-44
gb|AAC49278.1| sterol delta-7 reductase                               170  1e-41
gb|AAL14611.1|AF417293_1 putative delta-7-sterol reductase [Cast...   136  1e-31
emb|CAB44317.1| lamin B receptor [Xenopus laevis]                     110  1e-23
ref|NP_604448.1| lamin B receptor [Rattus norvegicus] gi|7513997...   108  5e-23

>ref|NP_175460.1| sterol delta7 reductase; protein id: At1g50430.1, supported by
           cDNA: gi_1245181, supported by cDNA: gi_20466245
           [Arabidopsis thaliana] gi|20140296|sp|Q9LDU6|ST7R_ARATH
           7-dehydrocholesterol reductase (7-DHC reductase) (Sterol
           delta-7-reductase) (Dwarf5 protein)
           gi|25336689|pir||F96540 sterol delta7 reductase
           [imported] - Arabidopsis thaliana
           gi|7542561|gb|AAF63498.1|AF239701_1 sterol delta7
           reductase [Arabidopsis thaliana]
           gi|9454565|gb|AAF87888.1|AC012561_21 sterol delta7
           reductase [Arabidopsis thaliana]
           gi|20466246|gb|AAM20440.1| sterol delta7 reductase
           [Arabidopsis thaliana] gi|23198074|gb|AAN15564.1| sterol
           delta7 reductase [Arabidopsis thaliana]
          Length = 432

 Score =  179 bits (453), Expect = 2e-44
 Identities = 79/89 (88%), Positives = 86/89 (95%)
 Frame = -1

Query: 521 GQTKRSLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKR 342
           G+TK SLLLTSGWWGL+RHFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKR
Sbjct: 344 GETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKR 403

Query: 341 DDDRCRSKYGKYWKLYCDKVPYRIIPGIY 255
           DDDRCRSKYGKYWKLYC+KV YRIIPGIY
Sbjct: 404 DDDRCRSKYGKYWKLYCEKVKYRIIPGIY 432

>gb|AAC49278.1| sterol delta-7 reductase
          Length = 430

 Score =  170 bits (430), Expect = 1e-41
 Identities = 77/89 (86%), Positives = 84/89 (93%)
 Frame = -1

Query: 521 GQTKRSLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKR 342
           G+TK SLLLTSGWWGL+RHFHYVPEIL+AFFWTVPALF++FL YFYV  LT+LLFDRAKR
Sbjct: 344 GETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYV--LTLLLFDRAKR 401

Query: 341 DDDRCRSKYGKYWKLYCDKVPYRIIPGIY 255
           DDDRCRSKYGKYWKLYC+KV YRIIPGIY
Sbjct: 402 DDDRCRSKYGKYWKLYCEKVKYRIIPGIY 430

>gb|AAL14611.1|AF417293_1 putative delta-7-sterol reductase [Castanea sativa]
          Length = 67

 Score =  136 bits (343), Expect = 1e-31
 Identities = 59/65 (90%), Positives = 65/65 (99%)
 Frame = -1

Query: 449 EILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCDKVPYRI 270
           +IL+AFFWTVPALFNHFLP+FYVIFLTILLFDRAKRDDDRCRSKYGKYWK++C+KVPYRI
Sbjct: 3   QILSAFFWTVPALFNHFLPFFYVIFLTILLFDRAKRDDDRCRSKYGKYWKVFCEKVPYRI 62

Query: 269 IPGIY 255
           IPGIY
Sbjct: 63  IPGIY 67

>emb|CAB44317.1| lamin B receptor [Xenopus laevis]
          Length = 620

 Score =  110 bits (274), Expect = 1e-23
 Identities = 48/84 (57%), Positives = 58/84 (68%)
 Frame = -1

Query: 506 SLLLTSGWWGLSRHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRC 327
           S LL SGWWG  RH +Y+ +I+ A  W +   FNH LPYFYVIFLT+LL DRA RD+ RC
Sbjct: 537 SKLLISGWWGFVRHPNYLGDIIMALAWCLACGFNHILPYFYVIFLTLLLIDRAARDEQRC 596

Query: 326 RSKYGKYWKLYCDKVPYRIIPGIY 255
           R KYG  W  YC  V YR++P +Y
Sbjct: 597 REKYGLDWDKYCQHVRYRLLPYVY 620

>ref|NP_604448.1| lamin B receptor [Rattus norvegicus] gi|7513997|pir||JC5567 lamin B
           receptor - rat gi|2204062|dbj|BAA20471.1| Rat NBP60
           [Rattus norvegicus]
          Length = 620

 Score =  108 bits (269), Expect = 5e-23
 Identities = 45/82 (54%), Positives = 57/82 (68%)
 Frame = -1

Query: 500 LLTSGWWGLSRHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRS 321
           LL SGWWG  RH +Y+ +++ A  W++P  FNH LPYFYVI+ T LL  R  RD+ +CR 
Sbjct: 539 LLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHILPYFYVIYFTALLIHREARDEHQCRR 598

Query: 320 KYGKYWKLYCDKVPYRIIPGIY 255
           KYG  W+ YC +VPYRI P IY
Sbjct: 599 KYGLAWEKYCQRVPYRIFPYIY 620

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 406,444,018
Number of Sequences: 1393205
Number of extensions: 7883124
Number of successful extensions: 18997
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 18635
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18993
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16731298976
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MF050d02_f BP030926 1 544
2 MPD092e10_f AV776050 25 316
3 SPD092b02_f BP051321 25 186
4 MPDL005g11_f AV776794 36 279
5 GNf091h02 BP074119 66 388
6 MWL024a09_f AV768968 84 498




Lotus japonicus
Kazusa DNA Research Institute