>KMC002259A_C01 KMC002259A_c01
ataatactgctatgattactaagaaattctgttAAATCAGATATTCAACACGTTTCTAAA
CATCTATTGAACTTTCATTCTACATAGACACCAAAAGCAGCAGTTACACCTGAGGCTATG
CTACATACAATATCACTCAAACATGATCTATACATCTCAATCACACAAACTATAAAACCA
GAGCATATAACAGCTACTTTAGGCCCAAGCCTCTCCTCCTCCTTCTCTAGTATTTACAAT
GCCAAGGTCTGCACGCTTCTTAGTAGACTTGCTCAGTTGAAACACAGGGTGTTGGCATAG
CCTGCGCACTCCTTTTCTCAATCTCAGCCAATGTTCACTGTCATATATACCTCTATATCT
ACTTTCTAAAGGACCACTCTCATATCTAGAAGGTGAGCTACCACCTGAAACAGATTCCAA
TGCGCCGCCTCCGGTTCCTTTTTCCTCAGAGCTCCAAACCTTCTCATCAACACACCATTC
CATTCTTGAGAACTCGCTTTTGTCAGGTGGCATTGTTGCACAAGTATCCACTGGTTGTGC
ACTAGATTCCTCCCTGTTATCTTTGAAAGGATGGGAATCACCTTTGCAGGAGGGAGAATT
TAAGAGTGATGAAAGCTGTCTCGCTTTCGCCATTGATTCATCTCTTTCTCGAAGGGTCTT
CAGAATCAACGTCTTAAGGAAGTTCATGACTTGCACCGCGTGAATCAATGCAGTCAAAGG
GTCGACCATCTGTGTCATGTTGGGGGCGAAAACCATAGCAATATTGCGTGCATTCATTTT
GTTGAACTGTTCATGCTCAACAACATCTGCCATCAAATTGATTGCCCAGTCAAGTAGTGC
AGCTTCAGTTgaagggagtaacttcacaagattagtacaatcctcttctgagttgcagtg
catcacttgttctggggtgagagaatcaag

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002259A_C01 KMC002259A_c01
         (930 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAC62625.1| rac GTPase activating protein 2 [Lotus japonicus]      495  e-139
ref|NP_182194.2| putative rac GTPase activating protein; protein...   204  2e-51
pir||C84906 probable rac GTPase activating protein [imported] - ...   204  2e-51
ref|NP_187756.1| putative rac GTPase activating protein; protein...   164  1e-39
dbj|BAC20714.1| similar to rac GTPase activating protein 3 [Oryz...   161  1e-38

>gb|AAC62625.1| rac GTPase activating protein 2 [Lotus japonicus]
          Length = 424

 Score =  495 bits (1274), Expect = e-139
 Identities = 243/243 (100%), Positives = 243/243 (100%)
 Frame = -1

Query: 930 LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV 751
           LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV
Sbjct: 182 LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV 241

Query: 750 FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLLNSPSCKGDSH 571
           FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLLNSPSCKGDSH
Sbjct: 242 FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLLNSPSCKGDSH 301

Query: 570 PFKDNREESSAQPVDTCATMPPDKSEFSRMEWCVDEKVWSSEEKGTGGGALESVSGGSSP 391
           PFKDNREESSAQPVDTCATMPPDKSEFSRMEWCVDEKVWSSEEKGTGGGALESVSGGSSP
Sbjct: 302 PFKDNREESSAQPVDTCATMPPDKSEFSRMEWCVDEKVWSSEEKGTGGGALESVSGGSSP 361

Query: 390 SRYESGPLESRYRGIYDSEHWLRLRKGVRRLCQHPVFQLSKSTKKRADLGIVNTREGGGE 211
           SRYESGPLESRYRGIYDSEHWLRLRKGVRRLCQHPVFQLSKSTKKRADLGIVNTREGGGE
Sbjct: 362 SRYESGPLESRYRGIYDSEHWLRLRKGVRRLCQHPVFQLSKSTKKRADLGIVNTREGGGE 421

Query: 210 AWA 202
           AWA
Sbjct: 422 AWA 424

>ref|NP_182194.2| putative rac GTPase activating protein; protein id: At2g46710.1
           [Arabidopsis thaliana] gi|26449717|dbj|BAC41982.1|
           putative rac GTPase activating protein [Arabidopsis
           thaliana] gi|28951019|gb|AAO63433.1| At2g46710
           [Arabidopsis thaliana]
          Length = 455

 Score =  204 bits (519), Expect = 2e-51
 Identities = 124/251 (49%), Positives = 150/251 (59%), Gaps = 8/251 (3%)
 Frame = -1

Query: 930 LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV 751
           LD LTPEQVM CN+EEDC+ LV LLP  E+A+LDWAI LMADVVEHEQFNKMNARN+AMV
Sbjct: 235 LDVLTPEQVMRCNTEEDCSRLVILLPPVESAILDWAIGLMADVVEHEQFNKMNARNVAMV 294

Query: 750 FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLLNSPSCKGDSH 571
           FAPNMTQM DPLTALIHAVQVMNFLKTLIL  L+ER+ + AKAR L    + PS + +S 
Sbjct: 295 FAPNMTQMADPLTALIHAVQVMNFLKTLILMNLKERENADAKARWLKKQTSDPSEEWESQ 354

Query: 570 ------PFKDNREESSAQPVDTCATMPPDKSEFSRMEWCVDEKVWSSEEKGTGGGALESV 409
                 P K N        V T   +  D           +E+ W+ +++    G L++ 
Sbjct: 355 HSEILSPEKPNNNNPKFLRVATLCRLEADN----------EEEFWNIKKRNDHEGVLDTS 404

Query: 408 SGGSSPSRYESGPLESRYRGIYDSEHWLRLRKGVRRLCQHPVFQLSKSTKKRADLGIVNT 229
           SG  +      GP                    V+RLC+HP+FQLSKSTKK     + N 
Sbjct: 405 SGNGN-----IGP--------------------VQRLCKHPLFQLSKSTKKAF---VSNR 436

Query: 228 REG--GGEAWA 202
            EG  G EAW+
Sbjct: 437 DEGRKGREAWS 447

>pir||C84906 probable rac GTPase activating protein [imported] - Arabidopsis
           thaliana gi|3831445|gb|AAC69928.1| putative rac GTPase
           activating protein [Arabidopsis thaliana]
          Length = 301

 Score =  204 bits (519), Expect = 2e-51
 Identities = 124/251 (49%), Positives = 150/251 (59%), Gaps = 8/251 (3%)
 Frame = -1

Query: 930 LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV 751
           LD LTPEQVM CN+EEDC+ LV LLP  E+A+LDWAI LMADVVEHEQFNKMNARN+AMV
Sbjct: 81  LDVLTPEQVMRCNTEEDCSRLVILLPPVESAILDWAIGLMADVVEHEQFNKMNARNVAMV 140

Query: 750 FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLLNSPSCKGDSH 571
           FAPNMTQM DPLTALIHAVQVMNFLKTLIL  L+ER+ + AKAR L    + PS + +S 
Sbjct: 141 FAPNMTQMADPLTALIHAVQVMNFLKTLILMNLKERENADAKARWLKKQTSDPSEEWESQ 200

Query: 570 ------PFKDNREESSAQPVDTCATMPPDKSEFSRMEWCVDEKVWSSEEKGTGGGALESV 409
                 P K N        V T   +  D           +E+ W+ +++    G L++ 
Sbjct: 201 HSEILSPEKPNNNNPKFLRVATLCRLEADN----------EEEFWNIKKRNDHEGVLDTS 250

Query: 408 SGGSSPSRYESGPLESRYRGIYDSEHWLRLRKGVRRLCQHPVFQLSKSTKKRADLGIVNT 229
           SG  +      GP                    V+RLC+HP+FQLSKSTKK     + N 
Sbjct: 251 SGNGN-----IGP--------------------VQRLCKHPLFQLSKSTKKAF---VSNR 282

Query: 228 REG--GGEAWA 202
            EG  G EAW+
Sbjct: 283 DEGRKGREAWS 293

>ref|NP_187756.1| putative rac GTPase activating protein; protein id: At3g11490.1
           [Arabidopsis thaliana]
           gi|12322911|gb|AAG51449.1|AC008153_22 putative rac
           GTPase activating protein; 62102-60058 [Arabidopsis
           thaliana]
          Length = 435

 Score =  164 bits (416), Expect = 1e-39
 Identities = 97/178 (54%), Positives = 119/178 (66%), Gaps = 10/178 (5%)
 Frame = -1

Query: 930 LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV 751
           LDSL+PEQVM   SE++C  LV+LLPSTEA+LLDWAINLMADVVE EQ NKMNARNIAMV
Sbjct: 223 LDSLSPEQVMESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMV 282

Query: 750 FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLL------NSPS 589
           FAPNMTQM+DPLTAL++AVQVMNFLKTLI+KTL++R ES  K    S+        +  S
Sbjct: 283 FAPNMTQMLDPLTALMYAVQVMNFLKTLIVKTLKDRKESRDKLVPASNPSPRDHNGDQSS 342

Query: 588 CKGDSHPFKDNREES----SAQPVDTCATMPPDKSEFSRMEWCVDEKVWSSEEKGTGG 427
            +   H  K N+EE+     A+  D   +   ++ E +     VD K  S     +GG
Sbjct: 343 SRQLLHLMKANKEETLDNFEAEMKDKEESADEEEEECAESVELVDIKKSSLVNNSSGG 400

>dbj|BAC20714.1| similar to rac GTPase activating protein 3 [Oryza sativa (japonica
           cultivar-group)]
          Length = 487

 Score =  161 bits (408), Expect = 1e-38
 Identities = 79/120 (65%), Positives = 96/120 (79%)
 Frame = -1

Query: 930 LDSLTPEQVMHCNSEEDCTNLVKLLPSTEAALLDWAINLMADVVEHEQFNKMNARNIAMV 751
           LDSL+PEQV+HCN+EE+C  LV+LLP T+AALL+W +  MADVVE E+ NKMNARN+AMV
Sbjct: 206 LDSLSPEQVLHCNTEEECVELVRLLPPTQAALLNWVVEFMADVVEEEESNKMNARNVAMV 265

Query: 750 FAPNMTQMVDPLTALIHAVQVMNFLKTLILKTLRERDESMAKARQLSSLLNSPSCKGDSH 571
           FAPNMTQM DPLTAL+HAVQVMN LKTLILKTLRER+   ++   +SS  +S     + H
Sbjct: 266 FAPNMTQMSDPLTALMHAVQVMNLLKTLILKTLREREHDESEYSAISSQSSSSDELDEMH 325

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 764,509,176
Number of Sequences: 1393205
Number of extensions: 16899155
Number of successful extensions: 56293
Number of sequences better than 10.0: 168
Number of HSP's better than 10.0 without gapping: 52390
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 56142
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 51582455952
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF010f11_f BP028766 1 506 2 MR064e08_f BP080919 34 398 3 GENf049e08 BP060425 48 501 4 GNf030a05 BP069508 86 557 5 MWM062f07_f AV765693 501 932

---