KMC002203A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC002203A_C01 KMC002203A_c01
aactGATTAAAGCGAGAGAGAGAAGAGAAGAAAATGGCAAACTTTCCAGTTGTTGACATG
TCCAAGCTTAACACCGAAGAGAGAGTAGCAACCATGGAGTTGATAAAAGATGCTTGTGAG
AACTGGGGTTTCTTTGAGCTGGTGAACCATGAGATATCCACTGAGTTTATGGACACTGTG
GAAAGGCTCACAAAGGAGCACTACAAAAGGTTCATGGAGCAAAGGTTTAAAGAAATGGTT
GCCACCAAGGGTCTTGAGACTGTTCAGTCAGAAATCGATGACTTGGACTGGGAAAGCACC
TTCTTTTTGCGCCACCTTCCATCCTCCAACATTTCAGAGGTTCCAGATCTTGATGAAGAT
TACAGGAAGGTGATGAAGGAATTTGCAGTGAAGTTGGAGAAACTGGCAGAGAATCTTCTA
GACTTGCTGTGTGAGAATCTTGGGCTGGAGAAAGGGTACCTGAAGAAGGTGTTCTATGGT
TCCAAGGGACCAAATTTTGGGACCAAAGTTAGCAACTACCCTCCT
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002203A_C01 KMC002203A_c01
(525 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P31239|ACCO_PEA 1-aminocyclopropane-1-carboxylate oxidase (AC... 297 4e-80
pir||T10813 1-aminocyclopropane-1-carboxylate oxidase (EC 1.4.3.... 291 2e-78
dbj|BAA76387.1| ACC oxidase [Pyrus pyrifolia] 291 2e-78
emb|CAA67216.1| ACC oxidase [Malus x domestica] gi|2440086|emb|C... 288 3e-77
sp|Q00985|ACC1_MALDO 1-aminocyclopropane-1-carboxylate oxidase 1... 286 8e-77
>sp|P31239|ACCO_PEA 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase)
(Ethylene-forming enzyme) (EFE) gi|7433199|pir||T06544
1-aminocyclopropane-1-carboxylate oxidase (EC 1.4.3.-) -
garden pea gi|169041|gb|AAA33644.1|
1-aminocyclopropane-1-carboxylate oxidase
Length = 317
Score = 297 bits (761), Expect = 4e-80
Identities = 142/164 (86%), Positives = 152/164 (92%)
Frame = +1
Query: 34 MANFPVVDMSKLNTEERVATMELIKDACENWGFFELVNHEISTEFMDTVERLTKEHYKRF 213
M NFP+VDM KLNTE+R +TMELIKDACENWGFFE VNH IS E MDTVE+LTKEHYK+
Sbjct: 1 MENFPIVDMGKLNTEDRKSTMELIKDACENWGFFECVNHGISIEMMDTVEKLTKEHYKKC 60
Query: 214 MEQRFKEMVATKGLETVQSEIDDLDWESTFFLRHLPSSNISEVPDLDEDYRKVMKEFAVK 393
MEQRFKEMVATKGLE VQSEIDDLDWESTFFLRHLP S+ISE+PDLD+DYRKVMKEFA+K
Sbjct: 61 MEQRFKEMVATKGLECVQSEIDDLDWESTFFLRHLPVSSISEIPDLDDDYRKVMKEFALK 120
Query: 394 LEKLAENLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 525
LE+LAE LLDLLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPP
Sbjct: 121 LEELAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPP 164
>pir||T10813 1-aminocyclopropane-1-carboxylate oxidase (EC 1.4.3.-) ACO1 - mung
bean gi|458338|gb|AAC48921.1|
1-aminocylopropane-1-carboxylate oxidase homolog [Vigna
radiata] gi|12802439|gb|AAK07883.1|AF315316_1 ACC
oxidase [Vigna radiata] gi|1093118|prf||2102361A
aminocyclopropane carboxylate oxidase
Length = 317
Score = 291 bits (746), Expect = 2e-78
Identities = 140/164 (85%), Positives = 151/164 (91%)
Frame = +1
Query: 34 MANFPVVDMSKLNTEERVATMELIKDACENWGFFELVNHEISTEFMDTVERLTKEHYKRF 213
MANFPVVDM KLNTEER ME+IKDACENWGFFELVNH IS E MDTVE+LTKEHYK+
Sbjct: 1 MANFPVVDMGKLNTEERGTAMEMIKDACENWGFFELVNHGISIELMDTVEKLTKEHYKKT 60
Query: 214 MEQRFKEMVATKGLETVQSEIDDLDWESTFFLRHLPSSNISEVPDLDEDYRKVMKEFAVK 393
MEQRFKEMVA KGLE+VQSEI+DLDWESTFFLRHLP SN+SE DLD+DYRK+MK+FA +
Sbjct: 61 MEQRFKEMVANKGLESVQSEINDLDWESTFFLRHLPVSNVSENTDLDQDYRKIMKQFAEE 120
Query: 394 LEKLAENLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 525
LEKLAE+LLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP
Sbjct: 121 LEKLAEHLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 164
>dbj|BAA76387.1| ACC oxidase [Pyrus pyrifolia]
Length = 314
Score = 291 bits (746), Expect = 2e-78
Identities = 140/164 (85%), Positives = 151/164 (91%)
Frame = +1
Query: 34 MANFPVVDMSKLNTEERVATMELIKDACENWGFFELVNHEISTEFMDTVERLTKEHYKRF 213
MA FPVVD+S +N EERVAT+E I DACENWGFFELVNH ISTE +DTVE++TK+HYK+
Sbjct: 1 MATFPVVDLSLVNGEERVATLEKINDACENWGFFELVNHGISTELLDTVEKMTKDHYKKT 60
Query: 214 MEQRFKEMVATKGLETVQSEIDDLDWESTFFLRHLPSSNISEVPDLDEDYRKVMKEFAVK 393
MEQRFKEMVA KGL+ VQSEI DLDWESTFFLRHLPSSNISEVPDL+EDYRK MKEFAV+
Sbjct: 61 MEQRFKEMVAAKGLDAVQSEIHDLDWESTFFLRHLPSSNISEVPDLEEDYRKTMKEFAVE 120
Query: 394 LEKLAENLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 525
LEKLAE LLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP
Sbjct: 121 LEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 164
>emb|CAA67216.1| ACC oxidase [Malus x domestica] gi|2440086|emb|CAA04895.1| ACC
oxidase [Malus x domestica]
Length = 314
Score = 288 bits (737), Expect = 3e-77
Identities = 137/164 (83%), Positives = 150/164 (90%)
Frame = +1
Query: 34 MANFPVVDMSKLNTEERVATMELIKDACENWGFFELVNHEISTEFMDTVERLTKEHYKRF 213
MA FPVVD+S +N EER AT+E I DACENWGFFELVNH +STE +DTVE++TK+HYK+
Sbjct: 1 MATFPVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKT 60
Query: 214 MEQRFKEMVATKGLETVQSEIDDLDWESTFFLRHLPSSNISEVPDLDEDYRKVMKEFAVK 393
MEQRFKEMVA KGL+ VQSEI DLDWESTFFLRHLPSSNISE+PDL+EDYRK MKEFAV+
Sbjct: 61 MEQRFKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEDYRKTMKEFAVE 120
Query: 394 LEKLAENLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 525
LEKLAE LLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP
Sbjct: 121 LEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 164
>sp|Q00985|ACC1_MALDO 1-aminocyclopropane-1-carboxylate oxidase 1 (ACC oxidase 1)
(Ethylene-forming enzyme) (EFE) (Protein AP4) (PAE12)
gi|81731|pir||S22513 ethylene-forming enzyme - apple
tree gi|19545|emb|CAA43662.1| ethylene related [Malus x
domestica] gi|168337|gb|AAA33412.1| ripening-related
protein gi|3153159|emb|CAA74328.1| ACC oxidase [Malus x
domestica] gi|3641491|gb|AAC36461.1| ACC oxidase [Malus
x domestica] gi|384329|prf||1905416A aminocyclopropane
carboxylate oxidase
Length = 314
Score = 286 bits (733), Expect = 8e-77
Identities = 136/164 (82%), Positives = 150/164 (90%)
Frame = +1
Query: 34 MANFPVVDMSKLNTEERVATMELIKDACENWGFFELVNHEISTEFMDTVERLTKEHYKRF 213
MA FPVVD+S +N EER AT+E I DACENWGFFELVNH +STE +DTVE++TK+HYK+
Sbjct: 1 MATFPVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKT 60
Query: 214 MEQRFKEMVATKGLETVQSEIDDLDWESTFFLRHLPSSNISEVPDLDEDYRKVMKEFAVK 393
MEQRFKEMVA KGL+ VQSEI DLDWESTFFLRHLPSSNISE+PDL+E+YRK MKEFAV+
Sbjct: 61 MEQRFKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFAVE 120
Query: 394 LEKLAENLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 525
LEKLAE LLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP
Sbjct: 121 LEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPP 164
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 488,916,190
Number of Sequences: 1393205
Number of extensions: 10973657
Number of successful extensions: 40081
Number of sequences better than 10.0: 553
Number of HSP's better than 10.0 without gapping: 37872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39820
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
GENf044e02 |
BP060209 |
1 |
371 |
| 2 |
GENf076a10 |
BP061598 |
5 |
460 |
| 3 |
MWM022a05_f |
AV764963 |
19 |
420 |
| 4 |
MF100c05_f |
BP033488 |
20 |
526 |
| 5 |
GNf060a09 |
BP071798 |
30 |
441 |
| 6 |
MPD023a01_f |
AV771551 |
33 |
114 |
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Lotus japonicus
Kazusa DNA Research Institute