Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002202A_C01 KMC002202A_c01
(625 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_567663.1| HSP associated protein like; protein id: At4g22... 144 9e-35
pir||T04562 hypothetical protein T12H17.60 - Arabidopsis thalian... 125 6e-29
gb|AAA80493.1| similar to Plasmodium berghei heat shock related ... 102 3e-21
gb|AAK14819.1| hsp70-like protein [Plasmodium chabaudi] 101 9e-21
ref|NP_703618.1| hsp70 interacting protein, putative [Plasmodium... 100 3e-20
>ref|NP_567663.1| HSP associated protein like; protein id: At4g22670.1, supported by
cDNA: 35786., supported by cDNA: gi_16648866
[Arabidopsis thaliana] gi|16648867|gb|AAL24285.1| HSP
associated protein like [Arabidopsis thaliana]
gi|21593067|gb|AAM65016.1| HSP associated protein like
[Arabidopsis thaliana] gi|28058906|gb|AAO29967.1| HSP
associated protein like [Arabidopsis thaliana]
Length = 441
Score = 144 bits (364), Expect(2) = 9e-35
Identities = 80/125 (64%), Positives = 85/125 (68%), Gaps = 25/125 (20%)
Frame = -2
Query: 624 MPGGFPGG--GMPGGFPGG-----GMPGGFPGG-------GMPGGFPG--GGMPGGFPGG 493
MPGGFPGG GMPGGFPGG GMPGGFPGG GM GG PG GGMP G GG
Sbjct: 305 MPGGFPGGMGGMPGGFPGGMGGMGGMPGGFPGGMGGGMPAGMGGGMPGMGGGMPAGMGGG 364
Query: 492 GMPG---------GVPGNVDFSKLLNDPDLMAAFSDPEIMAALHDVMKNPATL*LSITGN 340
GMPG G+PG +DFSK+LNDP+LM AFSDPE+MAAL DVMKNPA L N
Sbjct: 365 GMPGAGGGMPGGGGMPGGMDFSKILNDPELMTAFSDPEVMAALQDVMKNPANL-AKHQAN 423
Query: 339 PKVAP 325
PKVAP
Sbjct: 424 PKVAP 428
Score = 46.2 bits (108), Expect = 3e-04
Identities = 22/36 (61%), Positives = 23/36 (63%), Gaps = 6/36 (16%)
Frame = -2
Query: 552 GGGMPGGFPGGGMPGGFPG------GGMPGGVPGNV 463
GGG PGG PGG PGG PG GGMPGG PG +
Sbjct: 290 GGGFPGGMPGG-FPGGMPGGFPGGMGGMPGGFPGGM 324
Score = 37.4 bits (85), Expect = 0.16
Identities = 26/61 (42%), Positives = 27/61 (43%), Gaps = 8/61 (13%)
Frame = +2
Query: 467 FPGTPPGMPPPGNPPGMP------PPGNPPGMPPPGNPPGMPPPGKPPGMPP--PGNPPG 622
FPG PG P G P G P P G P GM G PG P G GMP G PG
Sbjct: 293 FPGGMPGGFPGGMPGGFPGGMGGMPGGFPGGMGGMGGMPGGFPGGMGGGMPAGMGGGMPG 352
Query: 623 I 625
+
Sbjct: 353 M 353
Score = 33.1 bits (74), Expect = 3.0
Identities = 25/63 (39%), Positives = 25/63 (39%), Gaps = 13/63 (20%)
Frame = +2
Query: 473 GTPPGMPPPGNPPGMPPPGNPPGMPP------PGNPPGMPP-------PGKPPGMPPPGN 613
G P GM G PG P G GMP PG GMP PG GMP G
Sbjct: 319 GFPGGMGGMGGMPGGFPGGMGGGMPAGMGGGMPGMGGGMPAGMGGGGMPGAGGGMPGGGG 378
Query: 614 PPG 622
PG
Sbjct: 379 MPG 381
Score = 31.6 bits (70), Expect = 8.8
Identities = 22/48 (45%), Positives = 22/48 (45%)
Frame = +2
Query: 479 PPGMPPPGNPPGMPPPGNPPGMPPPGNPPGMPPPGKPPGMPPPGNPPG 622
P G PG PG P G P G PG GM P G P GM G PG
Sbjct: 288 PSGGGFPGGMPGGFPGGMPGGF--PGGMGGM-PGGFPGGMGGMGGMPG 332
Score = 24.3 bits (51), Expect(2) = 9e-35
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -3
Query: 323 VIAKMMSKFGGP 288
VIAKMM KF GP
Sbjct: 429 VIAKMMGKFAGP 440
>pir||T04562 hypothetical protein T12H17.60 - Arabidopsis thaliana
gi|2827544|emb|CAA16552.1| HSP associated protein like
[Arabidopsis thaliana] gi|7269113|emb|CAB79222.1| HSP
associated protein like [Arabidopsis thaliana]
Length = 627
Score = 125 bits (313), Expect(2) = 6e-29
Identities = 67/96 (69%), Positives = 72/96 (74%), Gaps = 2/96 (2%)
Frame = -2
Query: 606 GGGMPGGFPGGGMPGGFPGGGMPGGFPGGGMPGGFPGGGMPGG--VPGNVDFSKLLNDPD 433
GGG PGG GGGMPG GGGMP G GGGMPG GGGMPGG +PG +DFSK+LNDP+
Sbjct: 525 GGGFPGGM-GGGMPG--MGGGMPAGMGGGGMPGA--GGGMPGGGGMPGGMDFSKILNDPE 579
Query: 432 LMAAFSDPEIMAALHDVMKNPATL*LSITGNPKVAP 325
LM AFSDPE+MAAL DVMKNPA L NPKVAP
Sbjct: 580 LMTAFSDPEVMAALQDVMKNPANL-AKHQANPKVAP 614
Score = 24.3 bits (51), Expect(2) = 6e-29
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -3
Query: 323 VIAKMMSKFGGP 288
VIAKMM KF GP
Sbjct: 615 VIAKMMGKFAGP 626
>gb|AAA80493.1| similar to Plasmodium berghei heat shock related protein, encoded
by GenBank Accession Number L04508, and Trypanosoma
cruzi heat shock protein 70, Swiss-Prot Accession Number
P05456
Length = 173
Score = 102 bits (255), Expect = 3e-21
Identities = 55/101 (54%), Positives = 65/101 (63%), Gaps = 16/101 (15%)
Frame = -2
Query: 624 MPGGFPGG-------GMPGGFPGG---GMPGGFPGG---GMPGGFPGG---GMPGGFPGG 493
MPGGFPGG GMPGGFPGG GMPGG PGG GMPGG PGG GMPGG P G
Sbjct: 77 MPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMP-G 135
Query: 492 GMPGGVPGNVDFSKLLNDPDLMAAFSDPEIMAALHDVMKNP 370
GMPGG+PG + LN P++ F++P+ + ++M NP
Sbjct: 136 GMPGGMPGGMPD---LNSPEMKELFNNPQFFQMMQNMMSNP 173
Score = 49.7 bits (117), Expect = 3e-05
Identities = 33/63 (52%), Positives = 33/63 (52%), Gaps = 11/63 (17%)
Frame = +2
Query: 467 FPGTPPGMPPPGNPPGMP---PPGNPPGMP---PPGNPPGMP---PPGKPPGMP--PPGN 613
FPG PG P G P GMP P G P GMP P G P GMP P G P GMP PG
Sbjct: 61 FPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPGG 120
Query: 614 PPG 622
PG
Sbjct: 121 MPG 123
Score = 48.9 bits (115), Expect = 5e-05
Identities = 32/70 (45%), Positives = 33/70 (46%), Gaps = 7/70 (10%)
Frame = +2
Query: 434 SGSFKSLLKSTFPGTPPGMPPPGNPPGMP--PPGNPPGMPPPGNPPGMP---PPGKPPGM 598
SG F + PG PG P G P G P PG PG P G P GMP P G P GM
Sbjct: 58 SGDFPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGM 117
Query: 599 P--PPGNPPG 622
P PG PG
Sbjct: 118 PGGMPGGMPG 127
Score = 48.5 bits (114), Expect = 7e-05
Identities = 28/55 (50%), Positives = 29/55 (51%), Gaps = 5/55 (9%)
Frame = +2
Query: 467 FPGTPPGMPPPGNPPGMP---PPGNPPGMPP--PGNPPGMPPPGKPPGMPPPGNP 616
FPG PG P G P GMP P G P GMP PG PG P G P GMP +P
Sbjct: 97 FPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPDLNSP 151
>gb|AAK14819.1| hsp70-like protein [Plasmodium chabaudi]
Length = 407
Score = 101 bits (251), Expect = 9e-21
Identities = 55/105 (52%), Positives = 65/105 (61%), Gaps = 20/105 (19%)
Frame = -2
Query: 624 MPGGFPGG-------GMPGGFPGG-------GMPGGFPG---GGMPGGFPGG---GMPGG 505
MPGGFPGG GMPGGFPGG GMPGG PG GGMPGG PGG GMPGG
Sbjct: 272 MPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPSGMPGG 331
Query: 504 FPGGGMPGGVPGNVDFSKLLNDPDLMAAFSDPEIMAALHDVMKNP 370
P GGMPGG+PG + LN P++ F++P+ + ++M NP
Sbjct: 332 MP-GGMPGGMPGGMPD---LNSPEMKELFNNPQFFQMMQNMMSNP 372
Score = 49.7 bits (117), Expect = 3e-05
Identities = 33/63 (52%), Positives = 33/63 (52%), Gaps = 11/63 (17%)
Frame = +2
Query: 467 FPGTPPGMPPPGNPPGMP---PPGNPPGMP---PPGNPPGMP---PPGKPPGMP--PPGN 613
FPG PG P G P GMP P G P GMP P G P GMP P G P GMP PG
Sbjct: 256 FPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPGG 315
Query: 614 PPG 622
PG
Sbjct: 316 MPG 318
Score = 48.9 bits (115), Expect = 5e-05
Identities = 32/70 (45%), Positives = 33/70 (46%), Gaps = 7/70 (10%)
Frame = +2
Query: 434 SGSFKSLLKSTFPGTPPGMPPPGNPPGMP--PPGNPPGMPPPGNPPGMP---PPGKPPGM 598
SG F + PG PG P G P G P PG PG P G P GMP P G P GM
Sbjct: 253 SGDFPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGM 312
Query: 599 P--PPGNPPG 622
P PG PG
Sbjct: 313 PGGMPGGMPG 322
Score = 48.1 bits (113), Expect = 9e-05
Identities = 31/66 (46%), Positives = 32/66 (47%), Gaps = 4/66 (6%)
Frame = +2
Query: 437 GSFKSLLKSTFPGTPPGMPPPGNPPGMPPPGNPPGMP--PPGNPPGMPPPGKPPGMP--P 604
G F + PG PG P G P GM P G P GMP PG PG P G P GMP
Sbjct: 278 GGFPGGMPGGMPGGFPGGMPGGMPGGM-PGGMPGGMPGGMPGGMPGGMPSGMPGGMPGGM 336
Query: 605 PGNPPG 622
PG PG
Sbjct: 337 PGGMPG 342
Score = 47.4 bits (111), Expect = 2e-04
Identities = 28/59 (47%), Positives = 29/59 (48%), Gaps = 9/59 (15%)
Frame = +2
Query: 467 FPGTPPGMPPPGNPPGMP-------PPGNPPGMPP--PGNPPGMPPPGKPPGMPPPGNP 616
FPG PG P G P GMP P G P GMP PG PG P G P GMP +P
Sbjct: 292 FPGGMPGGMPGGMPGGMPGGMPGGMPGGMPGGMPSGMPGGMPGGMPGGMPGGMPDLNSP 350
>ref|NP_703618.1| hsp70 interacting protein, putative [Plasmodium falciparum 3D7]
gi|23504857|emb|CAD51638.1| hsp70 interacting protein,
putative [Plasmodium falciparum 3D7]
Length = 458
Score = 99.8 bits (247), Expect = 3e-20
Identities = 55/109 (50%), Positives = 65/109 (59%), Gaps = 24/109 (22%)
Frame = -2
Query: 624 MPGGFPGG---GMPGGFPG---GGMPGGFPG---GGMPGGFPG---GGMPGGFPGG---- 493
MPGG PGG GMPGGFPG GGMPGG PG GGMPGG PG GGMPGG PGG
Sbjct: 315 MPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPGGMPSG 374
Query: 492 -------GMPGGVPGNVDFSKL-LNDPDLMAAFSDPEIMAALHDVMKNP 370
GMPGG+PG + LN P++ F++P+ + ++M NP
Sbjct: 375 MPGGMPSGMPGGMPGGMPGGMPDLNSPEMKELFNNPQFFQMMQNMMSNP 423
Score = 49.3 bits (116), Expect = 4e-05
Identities = 31/59 (52%), Positives = 31/59 (52%), Gaps = 7/59 (11%)
Frame = +2
Query: 467 FPGTPPGMPPPGNPPGMP---PPGNPPGMP--PPGNPPGMPPPGKPPGMP--PPGNPPG 622
FPG PG P G P GMP P G P GMP PG PG P G P GMP PG PG
Sbjct: 311 FPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPG 369
Score = 48.5 bits (114), Expect = 7e-05
Identities = 32/72 (44%), Positives = 34/72 (46%), Gaps = 8/72 (11%)
Frame = +2
Query: 434 SGSFKSLLKSTFPGTPPGMPPPGNPPGMP--PPGNPPGMPPPGNPPGMP---PPGKPPGM 598
SG F + PG PG P G P G P PG PG P G P GMP P G P GM
Sbjct: 308 SGDFPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGM 367
Query: 599 P---PPGNPPGI 625
P P G P G+
Sbjct: 368 PGGMPSGMPGGM 379
Score = 48.1 bits (113), Expect = 9e-05
Identities = 31/69 (44%), Positives = 33/69 (46%), Gaps = 9/69 (13%)
Frame = +2
Query: 437 GSFKSLLKSTFPGTPPGMPPPGNPPGMP---PPGNPPGMP---PPGNPPGMP---PPGKP 589
G F + PG PG P G P GMP P G P GMP P G P GMP P G P
Sbjct: 333 GGFPGGMPGGMPGGFPGGMPGGMPGGMPGGMPGGMPGGMPSGMPGGMPSGMPGGMPGGMP 392
Query: 590 PGMPPPGNP 616
GMP +P
Sbjct: 393 GGMPDLNSP 401
Score = 47.0 bits (110), Expect = 2e-04
Identities = 32/81 (39%), Positives = 36/81 (43%), Gaps = 8/81 (9%)
Frame = +2
Query: 407 SGSLNAAIRSGSFKSLLKSTFPGTPPGMPPPGNPPGMP---PPGNPPGMPP--PGNPPGM 571
S S +++ F PG PG P G P G P P G P GMP PG PG
Sbjct: 295 SDSSDSSYSEPDFSGDFPGGMPGGMPGGMPGGMPGGFPGGFPGGMPGGMPGGFPGGMPGG 354
Query: 572 PPPGKPPGMP---PPGNPPGI 625
P G P GMP P G P G+
Sbjct: 355 MPGGMPGGMPGGMPGGMPSGM 375
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 651,765,130
Number of Sequences: 1393205
Number of extensions: 21206743
Number of successful extensions: 658781
Number of sequences better than 10.0: 8822
Number of HSP's better than 10.0 without gapping: 69969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 319003
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25301904073
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
