Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002171A_C02 KMC002171A_c02
(1205 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_174485.1| secretory carrier membrane protein, putative; p... 328 e-109
gb|AAF36688.1|AF225922_1 secretory carrier membrane protein [Ory... 264 9e-88
ref|NP_172584.1| secretory carrier membrane protein, putative; p... 263 8e-83
gb|AAC82326.1| similarity to SCAMP37 [Pisum sativum] 282 9e-75
ref|NP_179680.1| putative secretory carrier-associated membrane ... 281 1e-74
>ref|NP_174485.1| secretory carrier membrane protein, putative; protein id:
At1g32050.1, supported by cDNA: 34837. [Arabidopsis
thaliana] gi|25403140|pir||G86444 probable secretory
carrier membrane protein [imported] - Arabidopsis
thaliana gi|12321473|gb|AAG50798.1|AC074309_15 secretory
carrier membrane protein, putative [Arabidopsis
thaliana] gi|21592989|gb|AAM64938.1| secretory carrier
membrane protein, putative [Arabidopsis thaliana]
Length = 264
Score = 328 bits (841), Expect(2) = e-109
Identities = 150/179 (83%), Positives = 163/179 (90%)
Frame = -1
Query: 938 GVPVDDKNWPPIFPIIHHDIANEIPVHAQRLQYLAFASWLGIVLCLVFNVVAVMVCWIRG 759
GV +DDKNWPP FPIIHHDIA EIPVHAQ+LQYLAFASWLGIVLCLVFNV+A MVCWI+G
Sbjct: 86 GVQIDDKNWPPFFPIIHHDIAKEIPVHAQKLQYLAFASWLGIVLCLVFNVIATMVCWIKG 145
Query: 758 GGVKIFFLAVIYALLGVPLSYVLWYRPLYRAMRTDSALKFGWFFMFYLLHIAFCIFAAIA 579
GGVKIFFLA IYAL+G PLSYVLWYRPLYRAMRTDSALKFGWFF YL+HI FCI AAIA
Sbjct: 146 GGVKIFFLATIYALIGCPLSYVLWYRPLYRAMRTDSALKFGWFFFTYLIHIGFCIVAAIA 205
Query: 578 PPVVFHGKSLTGILAATDVFSDHVLVGIFYLVGFGLFCLESLLSLWVIQKIYLFFRGHK 402
PP+ FHGKSLTG+LAA DV SD +L GIFY +GFGLFCLESLLSLWV+QKIYL+FRG+K
Sbjct: 206 PPIFFHGKSLTGVLAAIDVISDSLLAGIFYFIGFGLFCLESLLSLWVLQKIYLYFRGNK 264
Score = 91.3 bits (225), Expect(2) = e-109
Identities = 46/89 (51%), Positives = 62/89 (68%), Gaps = 2/89 (2%)
Frame = -3
Query: 1203 NRHHDPNPFDEDE--VNPFSKGAAPGSKSRIPPLASEALGLGQRHDATVDMP*DTTNEPK 1030
NRHHDPNPFDEDE VNPFSKG R+P AS + GQ DATVD+P D N+
Sbjct: 2 NRHHDPNPFDEDEEIVNPFSKGGG-----RVPA-ASRPVEYGQSLDATVDIPLDNMNDSS 55
Query: 1029 RKGQELGSWEADWKRREKDLKRREDAVSR 943
+K ++L WEA+ +++E D+KRRE+A+++
Sbjct: 56 QKQRKLADWEAELRKKEMDIKRREEAIAK 84
>gb|AAF36688.1|AF225922_1 secretory carrier membrane protein [Oryza sativa]
gi|15289879|dbj|BAB63575.1| secretory carrier membrane
protein [Oryza sativa (japonica cultivar-group)]
Length = 286
Score = 264 bits (675), Expect(2) = 9e-88
Identities = 115/177 (64%), Positives = 147/177 (82%)
Frame = -1
Query: 938 GVPVDDKNWPPIFPIIHHDIANEIPVHAQRLQYLAFASWLGIVLCLVFNVVAVMVCWIRG 759
G+ +++KNWPP FPIIHHDI+NEIP+H QR+QYLAF+S LG+ CL +N++A WI+G
Sbjct: 90 GIVIEEKNWPPFFPIIHHDISNEIPIHLQRMQYLAFSSLLGLAACLFWNIIATTAAWIKG 149
Query: 758 GGVKIFFLAVIYALLGVPLSYVLWYRPLYRAMRTDSALKFGWFFMFYLLHIAFCIFAAIA 579
GV I+ LA+IY + GVP +YVLWYRPLY AMRT+SALKFGWFF+FYL+HI FCI++A+A
Sbjct: 150 AGVMIWLLAIIYFISGVPGAYVLWYRPLYNAMRTESALKFGWFFLFYLIHILFCIWSAVA 209
Query: 578 PPVVFHGKSLTGILAATDVFSDHVLVGIFYLVGFGLFCLESLLSLWVIQKIYLFFRG 408
PP F GKSL GIL A DV ++ +VGIFY +GFGLFCLESLLS+ VIQ++Y++FRG
Sbjct: 210 PPFPFKGKSLAGILPAIDVIGNNAIVGIFYFIGFGLFCLESLLSVVVIQQVYMYFRG 266
Score = 83.2 bits (204), Expect(2) = 9e-88
Identities = 44/86 (51%), Positives = 60/86 (69%), Gaps = 1/86 (1%)
Frame = -3
Query: 1194 HDPNPFDEDEVNPFSKGAA-PGSKSRIPPLASEALGLGQRHDATVDMP*DTTNEPKRKGQ 1018
+D NPFDED+VNPF+ G+ P S SR+PPL E G ATVD+P D+T + K+K +
Sbjct: 5 YDRNPFDEDDVNPFAGGSVPPASNSRMPPLPHEP-GFYNDRGATVDIPLDSTKDMKKKEK 63
Query: 1017 ELGSWEADWKRREKDLKRREDAVSRA 940
EL + EA+ +RE +L+RRE+A SRA
Sbjct: 64 ELQAKEAELNKRESELRRREEAASRA 89
>ref|NP_172584.1| secretory carrier membrane protein, putative; protein id:
At1g11180.1 [Arabidopsis thaliana]
Length = 291
Score = 263 bits (671), Expect(2) = 8e-83
Identities = 117/177 (66%), Positives = 145/177 (81%)
Frame = -1
Query: 938 GVPVDDKNWPPIFPIIHHDIANEIPVHAQRLQYLAFASWLGIVLCLVFNVVAVMVCWIRG 759
G+ ++ KNWP FP+IHHDIANEI V QRLQY+AFA++LG+VL L +N++AV WI+G
Sbjct: 95 GIVIEAKNWPTFFPLIHHDIANEILVRLQRLQYIAFATYLGLVLALFWNIIAVTTAWIKG 154
Query: 758 GGVKIFFLAVIYALLGVPLSYVLWYRPLYRAMRTDSALKFGWFFMFYLLHIAFCIFAAIA 579
GV I+ LAVIY + GVP YVLWYRPLYRA R+DSA FGWFF+FY+LHI FC+FAA+A
Sbjct: 155 EGVTIWLLAVIYFISGVPGGYVLWYRPLYRAFRSDSAFNFGWFFLFYMLHILFCLFAAVA 214
Query: 578 PPVVFHGKSLTGILAATDVFSDHVLVGIFYLVGFGLFCLESLLSLWVIQKIYLFFRG 408
PP+VF GKSL GIL A DV S LVGIFY +GFGLFCLES++S+WVIQ++Y++FRG
Sbjct: 215 PPIVFKGKSLAGILPAIDVLSAQALVGIFYFIGFGLFCLESVVSIWVIQQVYMYFRG 271
Score = 68.2 bits (165), Expect(2) = 8e-83
Identities = 44/92 (47%), Positives = 59/92 (63%), Gaps = 7/92 (7%)
Frame = -3
Query: 1194 HDPNPFDE-DEVNPFSK-GAAPG-SKSRIPPLASEALGLGQRHDATVDMP*DT----TNE 1036
+D N FDE DEVNPF+ G+ P S SR+ PL E G G + TVD+P D +
Sbjct: 5 YDRNTFDEQDEVNPFANPGSVPAASNSRLSPLPPEPAGFG--YGRTVDIPLDRPGSGAQD 62
Query: 1035 PKRKGQELGSWEADWKRREKDLKRREDAVSRA 940
K+K +EL + EAD +RRE+DLKR++DA +RA
Sbjct: 63 LKKKEKELQAKEADLRRREQDLKRKQDAAARA 94
>gb|AAC82326.1| similarity to SCAMP37 [Pisum sativum]
Length = 289
Score = 282 bits (721), Expect = 9e-75
Identities = 126/197 (63%), Positives = 160/197 (80%)
Frame = -1
Query: 998 QIGKGEKRI*KEERMLYLERGVPVDDKNWPPIFPIIHHDIANEIPVHAQRLQYLAFASWL 819
++ K E+ + ++E + G+ +++KNWPP FPIIHHDI NEIP+H + LQY+AF S L
Sbjct: 73 ELRKREQEVRRKEEAI-ARAGIVIEEKNWPPFFPIIHHDITNEIPIHLRTLQYVAFFSLL 131
Query: 818 GIVLCLVFNVVAVMVCWIRGGGVKIFFLAVIYALLGVPLSYVLWYRPLYRAMRTDSALKF 639
G+VLCL +NVV+V WI+G GVKI+FLA+IY + GVP +Y LWYRPLYRA RTDSA+KF
Sbjct: 132 GLVLCLTWNVVSVTAAWIKGEGVKIWFLAIIYFIAGVPGAYALWYRPLYRAFRTDSAIKF 191
Query: 638 GWFFMFYLLHIAFCIFAAIAPPVVFHGKSLTGILAATDVFSDHVLVGIFYLVGFGLFCLE 459
GWFFMFYLLHI FCI AA+APP+VF GKSLTGIL+A DV D+ LVGIFY +GFG FCLE
Sbjct: 192 GWFFMFYLLHIGFCILAAVAPPIVFKGKSLTGILSAIDVVGDYTLVGIFYFIGFGFFCLE 251
Query: 458 SLLSLWVIQKIYLFFRG 408
+L+S+WVIQ++Y+ FRG
Sbjct: 252 TLISIWVIQQVYMHFRG 268
Score = 74.7 bits (182), Expect = 3e-12
Identities = 36/87 (41%), Positives = 60/87 (68%), Gaps = 2/87 (2%)
Frame = -3
Query: 1194 HDPNPFDEDEVNPFS--KGAAPGSKSRIPPLASEALGLGQRHDATVDMP*DTTNEPKRKG 1021
+DPNPFDE++VNPFS + AA + SR PL + TVD+P DT+ + K+K
Sbjct: 5 YDPNPFDEEQVNPFSNPRSAASATNSRPAPLNPDRADYNYGFGPTVDIPLDTSTDGKKKE 64
Query: 1020 QELGSWEADWKRREKDLKRREDAVSRA 940
++L + EA+ ++RE++++R+E+A++RA
Sbjct: 65 RDLQAKEAELRKREQEVRRKEEAIARA 91
>ref|NP_179680.1| putative secretory carrier-associated membrane protein; protein id:
At2g20840.1 [Arabidopsis thaliana]
gi|25411996|pir||A84594 hypothetical protein At2g20840
[imported] - Arabidopsis thaliana
gi|4454464|gb|AAD20911.1| putative secretory
carrier-associated membrane protein [Arabidopsis
thaliana]
Length = 282
Score = 281 bits (720), Expect = 1e-74
Identities = 123/197 (62%), Positives = 164/197 (82%)
Frame = -1
Query: 998 QIGKGEKRI*KEERMLYLERGVPVDDKNWPPIFPIIHHDIANEIPVHAQRLQYLAFASWL 819
++ + E+ I ++E + + G+ +++KNWPP FP+IHHDI+NEIP+H QR+QY+AF S L
Sbjct: 67 ELKRREQEIKRKEDAI-AQAGIVIEEKNWPPFFPLIHHDISNEIPIHLQRIQYVAFTSML 125
Query: 818 GIVLCLVFNVVAVMVCWIRGGGVKIFFLAVIYALLGVPLSYVLWYRPLYRAMRTDSALKF 639
G+V+CL++N+VAV WI+G G I+FLA+IY + GVP +YV+WYRPLYRAMRTDSALKF
Sbjct: 126 GLVVCLLWNIVAVTTAWIKGEGPTIWFLAIIYFISGVPGAYVMWYRPLYRAMRTDSALKF 185
Query: 638 GWFFMFYLLHIAFCIFAAIAPPVVFHGKSLTGILAATDVFSDHVLVGIFYLVGFGLFCLE 459
GWFF YL HIAFC+FAA+APP++F GKSLTGIL A DV S ++LVGIFY +GFG FCLE
Sbjct: 186 GWFFFTYLFHIAFCVFAAVAPPIIFKGKSLTGILPAIDVLSGNILVGIFYFIGFGFFCLE 245
Query: 458 SLLSLWVIQKIYLFFRG 408
SL+S+WVIQ++Y++FRG
Sbjct: 246 SLVSIWVIQQVYMYFRG 262
Score = 62.0 bits (149), Expect = 2e-08
Identities = 33/86 (38%), Positives = 55/86 (63%), Gaps = 1/86 (1%)
Frame = -3
Query: 1194 HDPNPFDEDEVNPFSKGAA-PGSKSRIPPLASEALGLGQRHDATVDMP*DTTNEPKRKGQ 1018
+ + FD+ E+NPF+ + P + S++ PL E G AT+D+P D+ + K K +
Sbjct: 4 YQSHSFDDGEINPFANPTSVPAATSKLSPLPPEPYDRG----ATMDIPLDSGKDLKAKEK 59
Query: 1017 ELGSWEADWKRREKDLKRREDAVSRA 940
EL EA+ KRRE+++KR+EDA+++A
Sbjct: 60 ELREKEAELKRREQEIKRKEDAIAQA 85
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,077,766,291
Number of Sequences: 1393205
Number of extensions: 25284176
Number of successful extensions: 80540
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 69993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80355
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 75114989675
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
