Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002161A_C01 KMC002161A_c01
(558 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_190491.1| pectinesterase - like protein; protein id: At3g... 120 8e-44
gb|AAG40402.1|AF325050_1 AT3g49220 [Arabidopsis thaliana] 120 8e-44
ref|NP_200149.1| pectinesterase; protein id: At5g53370.1, suppor... 105 3e-39
pir||T49241 pectinesterase-like protein - Arabidopsis thaliana g... 96 4e-31
gb|AAK81875.1| pectin methylesterase PME1 [Vitis vinifera] 97 7e-31
>ref|NP_190491.1| pectinesterase - like protein; protein id: At3g49220.1, supported
by cDNA: gi_16648928 [Arabidopsis thaliana]
gi|11279196|pir||T45827 pectinesterase-like protein -
Arabidopsis thaliana gi|6723392|emb|CAB66401.1|
pectinesterase-like protein [Arabidopsis thaliana]
gi|16648929|gb|AAL24316.1| pectinesterase-like protein
[Arabidopsis thaliana]
Length = 598
Score = 120 bits (301), Expect(2) = 8e-44
Identities = 52/65 (80%), Positives = 59/65 (90%)
Frame = -3
Query: 556 LKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGP 377
L+A+N S +TYLGRPWKL+SRTVYMMSYIG HVH RGWLEWNTTFALDTLYYGEY+N GP
Sbjct: 489 LQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGP 548
Query: 376 GAAVG 362
G+ +G
Sbjct: 549 GSGLG 553
Score = 78.6 bits (192), Expect(2) = 8e-44
Identities = 35/46 (76%), Positives = 42/46 (91%)
Frame = -1
Query: 366 LGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 229
LGQRV+WPGYRVI S EA+RFTVA+FI G++WLPSTGV+FL+GLS
Sbjct: 552 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
>gb|AAG40402.1|AF325050_1 AT3g49220 [Arabidopsis thaliana]
Length = 144
Score = 120 bits (301), Expect(2) = 8e-44
Identities = 52/65 (80%), Positives = 59/65 (90%)
Frame = -3
Query: 556 LKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGP 377
L+A+N S +TYLGRPWKL+SRTVYMMSYIG HVH RGWLEWNTTFALDTLYYGEY+N GP
Sbjct: 35 LQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGP 94
Query: 376 GAAVG 362
G+ +G
Sbjct: 95 GSGLG 99
Score = 78.6 bits (192), Expect(2) = 8e-44
Identities = 35/46 (76%), Positives = 42/46 (91%)
Frame = -1
Query: 366 LGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 229
LGQRV+WPGYRVI S EA+RFTVA+FI G++WLPSTGV+FL+GLS
Sbjct: 98 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 143
>ref|NP_200149.1| pectinesterase; protein id: At5g53370.1, supported by cDNA:
gi_13507548, supported by cDNA: gi_15293286 [Arabidopsis
thaliana] gi|9759184|dbj|BAB09799.1| pectinesterase
[Arabidopsis thaliana]
gi|13507549|gb|AAK28637.1|AF360340_1 putative
pectinesterase [Arabidopsis thaliana]
gi|15293287|gb|AAK93754.1| putative pectinesterase
[Arabidopsis thaliana]
Length = 587
Score = 105 bits (261), Expect(2) = 3e-39
Identities = 46/65 (70%), Positives = 53/65 (80%)
Frame = -3
Query: 556 LKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGP 377
L+AS S+ TYLGRPWKLYSR VYMMS +GDH+ RGWLEWN FALD+LYYGEYMN G
Sbjct: 478 LEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGL 537
Query: 376 GAAVG 362
G+ +G
Sbjct: 538 GSGIG 542
Score = 78.6 bits (192), Expect(2) = 3e-39
Identities = 35/46 (76%), Positives = 41/46 (89%)
Frame = -1
Query: 366 LGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 229
+GQRV WPGY VITS +EAS+FTVAQFI G++WLPSTGV+F SGLS
Sbjct: 541 IGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
>pir||T49241 pectinesterase-like protein - Arabidopsis thaliana
gi|7649367|emb|CAB89048.1| pectinesterase-like protein
[Arabidopsis thaliana] gi|17979141|gb|AAL49828.1|
putative pectinesterase [Arabidopsis thaliana]
gi|20465719|gb|AAM20328.1| putative pectinesterase
[Arabidopsis thaliana]
Length = 527
Score = 95.5 bits (236), Expect(2) = 4e-31
Identities = 40/55 (72%), Positives = 47/55 (84%)
Frame = -3
Query: 529 TYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAV 365
TYLGRPWKLYSRTV+M +Y+ D ++ GWLEWN FALDTLYYGEYMN GPGA++
Sbjct: 427 TYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASL 481
Score = 60.8 bits (146), Expect(2) = 4e-31
Identities = 25/45 (55%), Positives = 34/45 (75%)
Frame = -1
Query: 366 LGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 232
L +RV WPGY V+ ++ EA+ FTV+Q I G WLPSTG+ F++GL
Sbjct: 481 LDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525
>gb|AAK81875.1| pectin methylesterase PME1 [Vitis vinifera]
Length = 531
Score = 97.4 bits (241), Expect(2) = 7e-31
Identities = 44/65 (67%), Positives = 48/65 (73%)
Frame = -3
Query: 556 LKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGP 377
L AS S +YLGRPWK YSRT+ M SYI D + GWLEWN FALDTLYYGEYMNYGP
Sbjct: 422 LLASVNSTLSYLGRPWKQYSRTIIMKSYISDAIRPEGWLEWNGDFALDTLYYGEYMNYGP 481
Query: 376 GAAVG 362
A +G
Sbjct: 482 SAGLG 486
Score = 58.2 bits (139), Expect(2) = 7e-31
Identities = 23/46 (50%), Positives = 34/46 (73%)
Frame = -1
Query: 366 LGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 229
LG RV WPG+ ++ ++ +A+ FTV +FI G WLPSTGV + +GL+
Sbjct: 485 LGSRVQWPGFHLLNNSAQAANFTVTEFIAGNLWLPSTGVKYSAGLA 530
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 559,573,085
Number of Sequences: 1393205
Number of extensions: 13730854
Number of successful extensions: 39316
Number of sequences better than 10.0: 209
Number of HSP's better than 10.0 without gapping: 37645
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39229
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
