KMC002120A_c03
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC002120A_C03 KMC002120A_c03
cacaataacactctCTTTCACCACTAAAAAAGCCACAGTAGATTGGTTCTAAACAATCCA
GTTCAATTTTACATTACAACATCTGCAACCAAGGAATGAATATTAATAACAAAATCGCCC
TTAACATGAATATATACCAGATACAGATTTTTTCAAGGAAGTGTGATATTACAAATGACT
TTACTTCTGCACAATGAAAAAAGCTATAAAGAATCCAGCACACTGCCACACATTTCAAAA
GTTATTGCATACCAATAACTTTTAAGAACCCTTATTACCTTACCATTTCGAAAGGGATGG
GCACAACAATAACATATGCTAGTAAATATTATCTAATTTAAGATGATGCAGCTTAGGTAG
ATGGGTTGAAAGCAACCTGCTCAGCACACGAAAGGAATTCATGGGGGCCAGCTGTTTCTC
GACCAACACCAAGAAGGTCAAGATAATAGAGGTAATGGGAAATGATCACGTTCATTGGGT
CAATATCACCTTGTCCACAAACACTCTCCCCATAGAGAACGTTCATTGTGGAGCCAAATC
CAGGAATCCTTTTCTCCATGGTGTCATTCTGGCTAGGCTTCCATTTTCCGACGAAGATAT
CATGGGCAGAGGGTTGGTACTTCTTGTTGGGTGTCATCCATCTCCAGATCGCAGCCTGGA
AAGCAAGTGTTGCATTCTGCTCAATGTATTCTGGATGGTCCAGTAGGTTCACCTTCAAGG
CTTCTCCAACTGCACCATAATTGTAGTTCCAGTAAATAGG
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002120A_C03 KMC002120A_c03
(760 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_172076.1| glycosyl hydrolase family 19 (chitinase); prote... 236 3e-61
ref|NP_188317.1| glycosyl hydrolase family 19 (chitinase); prote... 221 9e-57
dbj|BAC55635.1| OJ1081_B12.16 [Oryza sativa (japonica cultivar-g... 214 1e-54
pir||T46629 lp6 protein - loblolly pine gi|974294|gb|AAA75101.1|... 131 9e-30
pir||S26625 chitinase (EC 3.2.1.14) - potato gi|21495|emb|CAA479... 107 2e-22
>ref|NP_172076.1| glycosyl hydrolase family 19 (chitinase); protein id: At1g05850.1,
supported by cDNA: gi_12083323, supported by cDNA:
gi_14334487, supported by cDNA: gi_17226328, supported
by cDNA: gi_21280934 [Arabidopsis thaliana]
gi|25406872|pir||C86193 hypothetical protein [imported]
- Arabidopsis thaliana
gi|6850314|gb|AAF29391.1|AC009999_11 Contains similarity
to a basic endochitinase from Arabidopis thaliana
gb|AB023448, and contains a Chitinases class I PF|00182
domain. ESTs gb|AI995747, gb|AA728545, gb|Z26222,
gb|Z25683, gb|T88386, gb|T14122, gb|T04241, gb|N38122
come from this gene. [Arabidopsis thaliana]
gi|12083324|gb|AAG48821.1|AF332458_1 putative class I
chitinase [Arabidopsis thaliana]
gi|14334488|gb|AAK59442.1| putative class I chitinase
[Arabidopsis thaliana]
gi|17226329|gb|AAL37736.1|AF422178_1 chitinase-like
protein 1 [Arabidopsis thaliana]
gi|17226331|gb|AAL37737.1|AF422179_1 chitinase-like
protein 1 [Arabidopsis thaliana]
gi|21280935|gb|AAM44973.1| putative class I chitinase
[Arabidopsis thaliana]
Length = 321
Score = 236 bits (602), Expect = 3e-61
Identities = 109/140 (77%), Positives = 122/140 (86%)
Frame = -1
Query: 760 PIYWNYNYGAVGEALKVNLLDHPEYIEQNATLAFQAAIWRWMTPNKKYQPSAHDIFVGKW 581
PIYWN+NYGA GEALK +LL+HPEYIEQNATLAFQAAIWRWMTP K+ QPSAHDIFVG W
Sbjct: 180 PIYWNFNYGAAGEALKADLLNHPEYIEQNATLAFQAAIWRWMTPIKRAQPSAHDIFVGNW 239
Query: 580 KPSQNDTMEKRIPGFGSTMNVLYGESVCGQGDIDPMNVIISHYLYYLDLLGVGRETAGPH 401
KP++NDT+ KR P FGSTMNVLYGE CGQG IDPMN IISHYLY+LDL+G+GRE AGP+
Sbjct: 240 KPTKNDTLSKRGPTFGSTMNVLYGEYTCGQGSIDPMNNIISHYLYFLDLMGIGREDAGPN 299
Query: 400 EFLSCAEQVAFNPST*AASS 341
+ LSCAEQ FNPST +SS
Sbjct: 300 DELSCAEQKPFNPSTVPSSS 319
>ref|NP_188317.1| glycosyl hydrolase family 19 (chitinase); protein id: At3g16920.1,
supported by cDNA: gi_15724261, supported by cDNA:
gi_19699111 [Arabidopsis thaliana]
gi|7670022|dbj|BAA94976.1| basic chitinase [Arabidopsis
thaliana] gi|15724262|gb|AAL06524.1|AF412071_1
AT3g16920/K14A17_4 [Arabidopsis thaliana]
gi|19699112|gb|AAL90922.1| AT3g16920/K14A17_4
[Arabidopsis thaliana]
Length = 333
Score = 221 bits (563), Expect = 9e-57
Identities = 101/141 (71%), Positives = 117/141 (82%), Gaps = 1/141 (0%)
Frame = -1
Query: 760 PIYWNYNYGAVGEALKVNLLDHPEYIEQNATLAFQAAIWRWMTPNKKYQPSAHDIFVGKW 581
P+YWNYNYG GEALKV+LL HPEY+E NATLAFQAAIWRWMTP KK+ PSAHD+FVGKW
Sbjct: 188 PVYWNYNYGQTGEALKVDLLSHPEYLENNATLAFQAAIWRWMTPPKKHLPSAHDVFVGKW 247
Query: 580 KPSQNDTMEKRIPGFGSTMNVLYGESVCGQG-DIDPMNVIISHYLYYLDLLGVGRETAGP 404
KP++NDT KR PGFG+T+NVLYG+ +C G D D MN I+SHYLYYLDL+GVGRE AGP
Sbjct: 248 KPTKNDTAAKRTPGFGATINVLYGDQICNSGFDNDEMNNIVSHYLYYLDLIGVGREEAGP 307
Query: 403 HEFLSCAEQVAFNPST*AASS 341
HE LSCA+Q F+ S+ A S
Sbjct: 308 HEKLSCADQEPFSSSSSAPPS 328
>dbj|BAC55635.1| OJ1081_B12.16 [Oryza sativa (japonica cultivar-group)]
Length = 316
Score = 214 bits (545), Expect = 1e-54
Identities = 92/135 (68%), Positives = 116/135 (85%)
Frame = -1
Query: 760 PIYWNYNYGAVGEALKVNLLDHPEYIEQNATLAFQAAIWRWMTPNKKYQPSAHDIFVGKW 581
P+YWNYNYG +G+ +K +LL+HPE +EQNATLAF+AAIWRWMTP K+ QPSAHD+FVG W
Sbjct: 179 PVYWNYNYGIIGQGIKQDLLNHPELLEQNATLAFEAAIWRWMTPMKRKQPSAHDVFVGNW 238
Query: 580 KPSQNDTMEKRIPGFGSTMNVLYGESVCGQGDIDPMNVIISHYLYYLDLLGVGRETAGPH 401
KP++NDT+ KR PGFG+TMN+LYG+ +CGQG ID MNVI+SHY +YLDL+GVG + AG +
Sbjct: 239 KPTKNDTLSKRYPGFGATMNILYGDLICGQGSIDKMNVIVSHYQHYLDLMGVGSDKAGDN 298
Query: 400 EFLSCAEQVAFNPST 356
L CA+QVAFNPS+
Sbjct: 299 --LDCADQVAFNPSS 311
>pir||T46629 lp6 protein - loblolly pine gi|974294|gb|AAA75101.1| LP6
Length = 216
Score = 131 bits (330), Expect = 9e-30
Identities = 63/114 (55%), Positives = 79/114 (69%), Gaps = 1/114 (0%)
Frame = -1
Query: 700 DHPEYIEQNATLAFQAAIWRWMTPNKKYQPSAHDIFVGKWKPSQNDTMEKRIPGFGSTMN 521
DH EY+ +NATLAF AAIWRWMTP K QPSAH + VGKW P++NDT R+PGFG T+N
Sbjct: 29 DHAEYLSENATLAFAAAIWRWMTPMKVKQPSAHQVMVGKWVPTKNDTEALRLPGFGMTIN 88
Query: 520 VLYGESVCG-QGDIDPMNVIISHYLYYLDLLGVGRETAGPHEFLSCAEQVAFNP 362
+L ++ CG D MN I+HYL +LD + VGRE AG + + C+EQ NP
Sbjct: 89 ILKADAECGTDSDDKQMNTRIAHYLDFLDHMDVGRENAGDN--VDCSEQKVLNP 140
>pir||S26625 chitinase (EC 3.2.1.14) - potato gi|21495|emb|CAA47921.1|
chitinase; endochitinase [Solanum tuberosum]
Length = 264
Score = 107 bits (267), Expect = 2e-22
Identities = 53/130 (40%), Positives = 75/130 (56%)
Frame = -1
Query: 757 IYWNYNYGAVGEALKVNLLDHPEYIEQNATLAFQAAIWRWMTPNKKYQPSAHDIFVGKWK 578
I +NYNYG G A+ VNLL++P+ + +A ++F+ A+W WMTP + +PSAHD+ G+W
Sbjct: 139 ISYNYNYGPAGRAIGVNLLNNPDLVANDAVVSFKTALWFWMTPQQP-KPSAHDVITGRWS 197
Query: 577 PSQNDTMEKRIPGFGSTMNVLYGESVCGQGDIDPMNVIISHYLYYLDLLGVGRETAGPHE 398
PS D+ R+PGFG N++ G C +G M+ I Y Y +LGV P
Sbjct: 198 PSAADSAAGRVPGFGVITNIINGGIECSKGSNAQMDNRIGFYRRYCQILGV-----DPGN 252
Query: 397 FLSCAEQVAF 368
L CA Q F
Sbjct: 253 NLDCANQRPF 262
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 708,418,342
Number of Sequences: 1393205
Number of extensions: 16551021
Number of successful extensions: 46932
Number of sequences better than 10.0: 346
Number of HSP's better than 10.0 without gapping: 45183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46688
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 37158613404
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
MFB036h09_f |
BP036665 |
1 |
491 |
| 2 |
SPD054g01_f |
BP048331 |
46 |
613 |
| 3 |
MFB072f11_f |
BP039261 |
49 |
511 |
| 4 |
MFB052a02_f |
BP037739 |
49 |
514 |
| 5 |
MPD092c08_f |
AV776031 |
52 |
570 |
| 6 |
MF058f08_f |
BP031371 |
59 |
560 |
| 7 |
MF009f12_f |
BP028707 |
71 |
521 |
| 8 |
SPD005d11_f |
BP044396 |
74 |
546 |
| 9 |
MFB047e04_f |
BP037425 |
76 |
478 |
| 10 |
SPDL079c12_f |
BP056904 |
85 |
558 |
| 11 |
MPD004e02_f |
AV770260 |
95 |
429 |
| 12 |
MF030f02_f |
BP029864 |
102 |
615 |
| 13 |
SPD061g02_f |
BP048877 |
129 |
647 |
| 14 |
MPD081f10_f |
AV775339 |
136 |
646 |
| 15 |
SPD084g09_f |
BP050735 |
139 |
634 |
| 16 |
MPDL045h01_f |
AV778802 |
239 |
763 |
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Lotus japonicus
Kazusa DNA Research Institute