>KMC002111A_C03 KMC002111A_c03
GTACGGGCCCCCCTCGATGGTAGCAGTGAGAGTGAAGAGAAAAAAAATGGCTCCCAAAGC
TTTCATCATCTTTTTGTCTCTGGTTATCCCTTTCAGCACTGTCCCTTCTGAGCCCTTCTT
CTGCACAAGAAGATGTTCAGGACACTGGCCTTGCCATGAACTTCTACAAGGAAACATGTC
CTCAGGCTGAAGACATCATCACAGAACAAGTCAAGCTTCTCTACAAGCGCCACAAGAACA
CCGCTTTCTCCTGGCTCAGGAACATCTTCCATGATTGTGCTGTTCAAAGTTGTGATGCTT
CATGGTTGCTGGATTCCACAAGAAAAACCTTGTCTGAGAAGGAAACTGATAGGAGCTTTG
GGTTGAGGAACTTCAGGTACATTGAGACCATCAAGGAAGCTCTGGAGAGGGAGTGCCCTG
GAGTTGTTTCCTGTTCTGATATCCTTGTTCTCTCTGCCAGAGATGGCATTGCTGCGCTGG
GAGGACCCCATATCCCTCTTAGAACTGGAAGAAGGGATGGGAGAAGAAGCAGAGCTGATG
TGGTTGAGCAGTTTCTTCCTGACCACAATGAGTCCATTTCTGCAGTTCTTGACAAGTTTG
GTGCCATGGGAATTGACACCCTGGCGTGGTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002111A_C03 KMC002111A_c03
         (631 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAD37374.1|AF145348_1 peroxidase [Glycine max]                     317  6e-86
pir||T06227 peroxidase (EC 1.11.1.7) 2, cationic - soybean gi|39...   309  2e-83
pir||T10790 peroxidase (EC 1.11.1.7) - upland cotton gi|167367|g...   298  5e-80
emb|CAB71128.2| cationic peroxidase [Cicer arietinum]                 293  1e-78
dbj|BAB16317.1| secretory peroxidase [Avicennia marina]               278  3e-74

>gb|AAD37374.1|AF145348_1 peroxidase [Glycine max]
          Length = 336

 Score =  317 bits (813), Expect = 6e-86
 Identities = 162/189 (85%), Positives = 173/189 (90%)
 Frame = +3

Query: 54  PKLSSSFCLWLSLSALSLLSPSSAQEDVQDTGLAMNFYKETCPQAEDIITEQVKLLYKRH 233
           PK  +   + + +SALSL SPS A E  Q+ GL MNFYKE+CPQAEDIITEQVKLLYKRH
Sbjct: 3   PKGLTFLAVLICVSALSL-SPSVAGEG-QNNGLVMNFYKESCPQAEDIITEQVKLLYKRH 60

Query: 234 KNTAFSWLRNIFHDCAVQSCDASWLLDSTRKTLSEKETDRSFGLRNFRYIETIKEALERE 413
           KNTAFSWLRNIFHDCAVQSCDAS LLDSTR++LSEKETDRSFGLRNFRYIETIKEALERE
Sbjct: 61  KNTAFSWLRNIFHDCAVQSCDASLLLDSTRRSLSEKETDRSFGLRNFRYIETIKEALERE 120

Query: 414 CPGVVSCSDILVLSARDGIAALGGPHIPLRTGRRDGRRSRADVVEQFLPDHNESISAVLD 593
           CPGVVSC+DILVLSARDGI +LGGPHIPL+TGRRDGRRSRADVVEQFLPDHNESISAVLD
Sbjct: 121 CPGVVSCADILVLSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLD 180

Query: 594 KFGAMGIDT 620
           KFGAMGIDT
Sbjct: 181 KFGAMGIDT 189

>pir||T06227 peroxidase (EC 1.11.1.7) 2, cationic - soybean
           gi|3982596|gb|AAC83463.1| cationic peroxidase 2 [Glycine
           max]
          Length = 331

 Score =  309 bits (791), Expect = 2e-83
 Identities = 154/181 (85%), Positives = 165/181 (91%)
 Frame = +3

Query: 78  LWLSLSALSLLSPSSAQEDVQDTGLAMNFYKETCPQAEDIITEQVKLLYKRHKNTAFSWL 257
           ++L++   S LS S      +D GL MNFYKE+CPQAEDII EQVKLLYKRHKNTAFSWL
Sbjct: 7   IFLAVLCFSALSLSRCL--AEDNGLVMNFYKESCPQAEDIIKEQVKLLYKRHKNTAFSWL 64

Query: 258 RNIFHDCAVQSCDASWLLDSTRKTLSEKETDRSFGLRNFRYIETIKEALERECPGVVSCS 437
           RNIFHDCAVQSCDAS LLDSTR++LSEKETDRSFGLRNFRYIETIKEALERECPGVVSC+
Sbjct: 65  RNIFHDCAVQSCDASLLLDSTRRSLSEKETDRSFGLRNFRYIETIKEALERECPGVVSCA 124

Query: 438 DILVLSARDGIAALGGPHIPLRTGRRDGRRSRADVVEQFLPDHNESISAVLDKFGAMGID 617
           DILVLSARDGI +LGGPHIPL+TGRRDGRRSRADVVEQFLPDHNESISAVLDKFGAMGID
Sbjct: 125 DILVLSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDKFGAMGID 184

Query: 618 T 620
           T
Sbjct: 185 T 185

>pir||T10790 peroxidase (EC 1.11.1.7) - upland cotton gi|167367|gb|AAA99868.1|
           peroxidase
          Length = 332

 Score =  298 bits (762), Expect = 5e-80
 Identities = 143/174 (82%), Positives = 161/174 (92%)
 Frame = +3

Query: 99  LSLLSPSSAQEDVQDTGLAMNFYKETCPQAEDIITEQVKLLYKRHKNTAFSWLRNIFHDC 278
           LS  + S+  +D +D GL MNFYK++CPQAEDII EQVKLLYKRHKNTAFSWLRNIFHDC
Sbjct: 16  LSFSAVSAFAQDEEDQGLVMNFYKDSCPQAEDIIKEQVKLLYKRHKNTAFSWLRNIFHDC 75

Query: 279 AVQSCDASWLLDSTRKTLSEKETDRSFGLRNFRYIETIKEALERECPGVVSCSDILVLSA 458
           AVQSCDAS LLDSTR++LSEKETDRSFGLRNFRYIETIKEA+ERECPGVVSC+DILVLSA
Sbjct: 76  AVQSCDASLLLDSTRRSLSEKETDRSFGLRNFRYIETIKEAVERECPGVVSCADILVLSA 135

Query: 459 RDGIAALGGPHIPLRTGRRDGRRSRADVVEQFLPDHNESISAVLDKFGAMGIDT 620
           R+GI +LGGP+IPL+TGRRDGRRSRADVVE++LPDHNE+IS VLD+F AMGIDT
Sbjct: 136 REGIVSLGGPYIPLKTGRRDGRRSRADVVEEYLPDHNETISGVLDRFAAMGIDT 189

>emb|CAB71128.2| cationic peroxidase [Cicer arietinum]
          Length = 336

 Score =  293 bits (750), Expect = 1e-78
 Identities = 146/189 (77%), Positives = 165/189 (87%)
 Frame = +3

Query: 54  PKLSSSFCLWLSLSALSLLSPSSAQEDVQDTGLAMNFYKETCPQAEDIITEQVKLLYKRH 233
           P  +  F + LS S     + SSAQ   QD GL MN+YKE+CPQAE+II EQVKLLYKRH
Sbjct: 3   PNHAFIFLVLLSFSPQLFFTLSSAQ---QDNGLLMNYYKESCPQAEEIIKEQVKLLYKRH 59

Query: 234 KNTAFSWLRNIFHDCAVQSCDASWLLDSTRKTLSEKETDRSFGLRNFRYIETIKEALERE 413
           KNTAFSWLRNIFHDCAVQSCDAS LL STR++LSE+E DRSFGLRNFRYI+TIKEA+ERE
Sbjct: 60  KNTAFSWLRNIFHDCAVQSCDASLLLTSTRRSLSEQEHDRSFGLRNFRYIDTIKEAVERE 119

Query: 414 CPGVVSCSDILVLSARDGIAALGGPHIPLRTGRRDGRRSRADVVEQFLPDHNESISAVLD 593
           CPGVVSCSDILVLSARDGI +LGGP+IPL+TGRRDGR+SR D++E++LPDHNESISAVLD
Sbjct: 120 CPGVVSCSDILVLSARDGIVSLGGPYIPLKTGRRDGRKSRVDLLEEYLPDHNESISAVLD 179

Query: 594 KFGAMGIDT 620
           KFGAMGIDT
Sbjct: 180 KFGAMGIDT 188

>dbj|BAB16317.1| secretory peroxidase [Avicennia marina]
          Length = 331

 Score =  278 bits (712), Expect = 3e-74
 Identities = 136/186 (73%), Positives = 159/186 (85%)
 Frame = +3

Query: 60  LSSSFCLWLSLSALSLLSPSSAQEDVQDTGLAMNFYKETCPQAEDIITEQVKLLYKRHKN 239
           + SS    L   A+   S  SA  + +  GLAMN+Y+++CPQAE+II EQV+LLYKRHKN
Sbjct: 2   IMSSISKALFFFAILSFSSFSAFAENEGHGLAMNYYRDSCPQAEEIIKEQVQLLYKRHKN 61

Query: 240 TAFSWLRNIFHDCAVQSCDASWLLDSTRKTLSEKETDRSFGLRNFRYIETIKEALERECP 419
           TAFSWLRNIFHDC V+SCDAS LLDSTR+ LSEKETDRSFG+RNFRYIETIKEALERECP
Sbjct: 62  TAFSWLRNIFHDCFVESCDASLLLDSTRRMLSEKETDRSFGMRNFRYIETIKEALERECP 121

Query: 420 GVVSCSDILVLSARDGIAALGGPHIPLRTGRRDGRRSRADVVEQFLPDHNESISAVLDKF 599
           GVVSC+DILVLSARDGI +LGGP+IPL+TGRRDGR+SRA+++EQ LPDHNES+S VLD+F
Sbjct: 122 GVVSCADILVLSARDGIVSLGGPYIPLKTGRRDGRKSRAEILEQHLPDHNESLSVVLDRF 181

Query: 600 GAMGID 617
             MGID
Sbjct: 182 ANMGID 187

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 603,256,522
Number of Sequences: 1393205
Number of extensions: 13428639
Number of successful extensions: 51412
Number of sequences better than 10.0: 472
Number of HSP's better than 10.0 without gapping: 48123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 50933
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25870486187
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD044c08_f BP047496 1 621 2 MPD063h02_f AV774231 8 553 3 GNf063e06 BP072053 13 569 4 MPD073a04_f AV774763 14 542 5 SPDL076e09_f BP056718 16 434 6 SPD033h04_f BP046656 16 646 7 MPD066f10_f AV774411 17 560 8 MWM207c05_f AV767917 20 629 9 MFB015h12_f BP035065 22 625 10 MFB093f03_f BP040794 23 618 11 MR038b06_f BP078922 24 537 12 GNf064e09 BP072129 24 486 13 GNf063f06 BP072063 25 551 14 SPD100h02_f BP052011 28 645 15 SPD023f04_f BP045831 29 553 16 SPD092a06_f BP051315 30 568 17 MR074f09_f BP081708 32 389 18 SPDL097g07_f BP058118 36 630 19 MFBL023g12_f BP042428 37 574 20 MFB043b11_f BP037124 37 495 21 MR081h05_f BP082266 37 448 22 MFB038f04_f BP036800 38 621 23 MFB087h11_f BP040389 38 602 24 MFB056g08_f BP038083 39 627 25 SPD003d03_f BP044229 40 617 26 MFB096c09_f BP040987 41 574 27 MFB044d07_f BP037216 43 641 28 MFB005f07_f BP034277 44 651 29 MFB004d06_f BP034182 45 662 30 MFB060d07_f BP038349 46 612 31 MFB059c04_f BP038258 50 535 32 MPD064c04_f AV774255 55 590 33 MPD075h07_f AV774956 78 471 34 MPD073e04_f AV774787 82 563 35 MPD097h07_f AV776375 158 599

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