>KMC002077A_C01 KMC002077A_c01
actgaataagtgCAATATTATAGCATGAACCCGTAGTTAAATAAGGTCCTTTTTACATCA
TGGAAGTTGAGTCCCCAAACGACATCCAAAAAGGGGGAAATGCTAATAGAATATAAACTA
AGAAAAGGGCTTAGGGGCGAGACTGTTCCAATGATATGAGAGATCCAAAGGATCATATCA
TAACACAATATTGAAACATAAACAAAAACAAAAATTGAAATCAACACTACCTACTTCATT
AGAAGTTTAGAACAAGGAAAATATATTTTCTCTTTTGACAAAATTTCAGTGAAACACGGA
AAGAACTCAGCTCACAAGAGAAAAAAACTTAAAAAAGGAAGAAAAGGTAAACAATATTCC
GATGATGACTAAATTGCATGGTTTCTGTGTCTTCAAGAAATGTCCACTATCTGTAATAAG
ATGCCAAAATATGGAGCATAACTGCAGATGATGTGGTGATTATCAACTCCTGACTGTTGT
AAAGTGTAGCAGGAATAAAACCACCGGCAAACTCAATTATGTTCAAAATGAAAGTGTATC
CCAAGGATGAAAAGGCTATACATCCGAAAACCAAGGCCAGTGATCTTAACAATCGTTTTC
CCATACGCAGTCCTTCTCCTGTATCTTCACCCTCTTCGTTATATGTGGAGGTTACAGGAG
ATTCAAAGGATTTTAACTTTTCAAAAAGCCTATAAACAAAAGCAAAGGCTAAAAACTGCA
ACGGT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002077A_C01 KMC002077A_c01
         (725 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM65006.1| unknown [Arabidopsis thaliana]                         144  1e-33
ref|NP_563823.1| expressed protein; protein id: At1g08640.1, sup...   144  1e-33
gb|AAN31811.1| unknown protein [Arabidopsis thaliana]                 142  4e-33
pir||H86218 protein F22O13.12 [imported] - Arabidopsis thaliana ...   117  2e-25
pir||T00717 hypothetical protein F22O13.12 - Arabidopsis thaliana     117  2e-25

>gb|AAM65006.1| unknown [Arabidopsis thaliana]
          Length = 294

 Score =  144 bits (363), Expect = 1e-33
 Identities = 70/105 (66%), Positives = 86/105 (81%)
 Frame = -2

Query: 724 PLQFLAFAFVYRLFEKLKSFESPVTSTYNEEGEDTGEGLRMGKRLLRSLALVFGCIAFSS 545
           PLQF++F FVYR+FEKLKSFE+P +  YNEEGE++G GLRMGKRLLRSL+LVFG I  +S
Sbjct: 190 PLQFMSFVFVYRIFEKLKSFEAPSSPIYNEEGEESGRGLRMGKRLLRSLSLVFGSILLAS 249

Query: 544 LGYTFILNIIEFAGGFIPATLYNSQELIITTSSAVMLHILASYYR 410
           L YT  LN IE+ G  IP  LYN+QELI+T SSA ML+++AS+YR
Sbjct: 250 LAYTGFLNGIEYMGYSIPMVLYNNQELIVTASSAFMLYVIASFYR 294

>ref|NP_563823.1| expressed protein; protein id: At1g08640.1, supported by cDNA:
           35718., supported by cDNA: gi_13877838, supported by
           cDNA: gi_16323503 [Arabidopsis thaliana]
           gi|13877839|gb|AAK43997.1|AF370182_1 unknown protein
           [Arabidopsis thaliana] gi|16323504|gb|AAL15246.1|
           unknown protein [Arabidopsis thaliana]
          Length = 294

 Score =  144 bits (363), Expect = 1e-33
 Identities = 70/105 (66%), Positives = 86/105 (81%)
 Frame = -2

Query: 724 PLQFLAFAFVYRLFEKLKSFESPVTSTYNEEGEDTGEGLRMGKRLLRSLALVFGCIAFSS 545
           PLQF++F FVYR+FEKLKSFE+P +  YNEEGE++G GLRMGKRLLRSL+LVFG I  +S
Sbjct: 190 PLQFMSFVFVYRIFEKLKSFEAPSSPIYNEEGEESGRGLRMGKRLLRSLSLVFGSILLAS 249

Query: 544 LGYTFILNIIEFAGGFIPATLYNSQELIITTSSAVMLHILASYYR 410
           L YT  LN IE+ G  IP  LYN+QELI+T SSA ML+++AS+YR
Sbjct: 250 LAYTGFLNGIEYMGYSIPMVLYNNQELIVTASSAFMLYVIASFYR 294

>gb|AAN31811.1| unknown protein [Arabidopsis thaliana]
          Length = 294

 Score =  142 bits (359), Expect = 4e-33
 Identities = 69/105 (65%), Positives = 86/105 (81%)
 Frame = -2

Query: 724 PLQFLAFAFVYRLFEKLKSFESPVTSTYNEEGEDTGEGLRMGKRLLRSLALVFGCIAFSS 545
           PLQF++F FVYR+FEKLKSFE+P +  YNEEGE++G GLRMGKRLLRSL+LVFG I  +S
Sbjct: 190 PLQFMSFVFVYRIFEKLKSFEAPSSPIYNEEGEESGRGLRMGKRLLRSLSLVFGSILLAS 249

Query: 544 LGYTFILNIIEFAGGFIPATLYNSQELIITTSSAVMLHILASYYR 410
           L YT  LN IE+ G  IP  LYN+QELI+T SSA +L+++AS+YR
Sbjct: 250 LAYTGFLNGIEYMGYSIPMVLYNNQELIVTASSAFILYVIASFYR 294

>pir||H86218 protein F22O13.12 [imported] - Arabidopsis thaliana
           gi|9802577|gb|AAF99779.1|AC003981_29 F22O13.12
           [Arabidopsis thaliana]
          Length = 319

 Score =  117 bits (292), Expect = 2e-25
 Identities = 57/85 (67%), Positives = 68/85 (79%)
 Frame = -2

Query: 724 PLQFLAFAFVYRLFEKLKSFESPVTSTYNEEGEDTGEGLRMGKRLLRSLALVFGCIAFSS 545
           PLQF++F FVYR+FEKLKSFE+P +  YNEEGE++G GLRMGKRLLRSL+LVFG I  +S
Sbjct: 190 PLQFMSFVFVYRIFEKLKSFEAPSSPIYNEEGEESGRGLRMGKRLLRSLSLVFGSILLAS 249

Query: 544 LGYTFILNIIEFAGGFIPATLYNSQ 470
           L YT  LN IE+ G  IP  LYN+Q
Sbjct: 250 LAYTGFLNGIEYMGYSIPMVLYNNQ 274

>pir||T00717 hypothetical protein F22O13.12 - Arabidopsis thaliana
          Length = 364

 Score =  117 bits (292), Expect = 2e-25
 Identities = 57/85 (67%), Positives = 68/85 (79%)
 Frame = -2

Query: 724 PLQFLAFAFVYRLFEKLKSFESPVTSTYNEEGEDTGEGLRMGKRLLRSLALVFGCIAFSS 545
           PLQF++F FVYR+FEKLKSFE+P +  YNEEGE++G GLRMGKRLLRSL+LVFG I  +S
Sbjct: 235 PLQFMSFVFVYRIFEKLKSFEAPSSPIYNEEGEESGRGLRMGKRLLRSLSLVFGSILLAS 294

Query: 544 LGYTFILNIIEFAGGFIPATLYNSQ 470
           L YT  LN IE+ G  IP  LYN+Q
Sbjct: 295 LAYTGFLNGIEYMGYSIPMVLYNNQ 319

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 593,986,164
Number of Sequences: 1393205
Number of extensions: 12701493
Number of successful extensions: 33165
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 31740
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33112
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 34062062287
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD037g03_f BP046966 1 573 2 MF004d03_f BP028440 13 420 3 MFB065e11_f BP038726 86 639 4 MR092d01_f BP083073 153 506 5 GENf034h08 BP059821 194 618 6 SPD003g02_f BP044261 197 725 7 SPD060a02_f BP048736 220 708 8 MFB084d10_f BP040146 252 437

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