>KMC002065A_C02 KMC002065A_c02
GAAGAAAATAACTGTTACAAAAATGAATTTATATCATTACAATATAATAAAAAATCAAAA
AAATATGTTCAGGATGCTACAAGGGAATTGGATGGTGTGAGTCTCATTGGACGCCAAAAA
TATTGGAAACAGTAAGAGACAAATATCATATGCCTTTGAATGCACCACAATACCAACTAA
CAGCTTAACCAACCAGTTCACAACTTGCTATAAGAGTTTGATTGATGCCATGACTGATAC
TCAAGAAGATTGATGAAAGAGACAAGCAAAATCAAGTGCATTTTAAATCAACCTTTTATC
AGAACTCTTGAGGTTCAGGTTAAACCATTTCTTTTTGCCTGATTTATCCTTGCCATCTGA
CCCTCCTTCTTCCCCAGGGCTCTCACCTTTGGAATCTTCACCAGAAATTTTGCTAGAACT
TCCATTGCTTAAATTACTACCACTTCCACTGATATTGGTTGGCTTGACAATGGTGAAGGA
AGAATGGATACCGTCCCTTTGTTTCAGAAGCTCATCTACAGCCTGTTTCTCTTTTGTGTA
TCTGTCTGTCACTTCCTGATACCGTGCTAATGCCTTTCTATATTCTGAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002065A_C02 KMC002065A_c02
         (589 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_177004.1| ARG1 protein (Altered Response to Gravity); pro...   135  5e-31
dbj|BAB32957.1| putative ARG1 protein (Altered Response to Gravi...   103  2e-21
pir||T00641 hypothetical protein F3I6.4 - Arabidopsis thaliana g...    67  2e-10
ref|NP_173822.2| DNAJ-like heatshock protein; protein id: At1g24...    67  2e-10
dbj|BAC43485.1| unknown protein [Arabidopsis thaliana] gi|289731...    59  5e-08

>ref|NP_177004.1| ARG1 protein (Altered Response to Gravity); protein id:
           At1g68370.1, supported by cDNA: gi_4249661 [Arabidopsis
           thaliana] gi|25296042|pir||E96707 hypothetical protein
           T2E12.8 [imported] - Arabidopsis thaliana
           gi|4249662|gb|AAD13758.1| Altered Response to Gravity
           [Arabidopsis thaliana]
           gi|6714354|gb|AAF26045.1|AC015986_8 ARG1 protein
           (Altered Response to Gravity); 32591-35072 [Arabidopsis
           thaliana]
          Length = 410

 Score =  135 bits (339), Expect = 5e-31
 Identities = 70/102 (68%), Positives = 87/102 (84%), Gaps = 2/102 (1%)
 Frame = -2

Query: 588 SEYRKALARYQEVTDRYTKEKQAVDELLKQRDGIHSSFTIVKPTNISGSGSNLSNGSSSK 409
           +EYRKALAR+QEVT+RYT+EKQ VDELLKQRD IHS+F++VK    + SG+NLSNGSSSK
Sbjct: 313 TEYRKALARFQEVTNRYTQEKQTVDELLKQRDTIHSTFSVVK----TPSGNNLSNGSSSK 368

Query: 408 ISGEDSK--GESPGEEGGSDGKDKSGKKKWFNLNLKSSDKRL 289
             G++SK  G+S GEEGG++ +DKS K+KWFNLNLK SDK+L
Sbjct: 369 AQGDESKGDGDSAGEEGGTENRDKS-KRKWFNLNLKGSDKKL 409

>dbj|BAB32957.1| putative ARG1 protein (Altered Response to Gravity) [Oryza sativa
           (japonica cultivar-group)] gi|14495200|dbj|BAB60919.1|
           putative ARG1 protein (Altered Response to Gravity)
           [Oryza sativa (japonica cultivar-group)]
          Length = 403

 Score =  103 bits (257), Expect = 2e-21
 Identities = 57/100 (57%), Positives = 71/100 (71%), Gaps = 1/100 (1%)
 Frame = -2

Query: 588 SEYRKALARYQEVTDRYTKEKQAVDELLKQRDGIHSSFTIVKP-TNISGSGSNLSNGSSS 412
           +EYRKALAR+QEVT+RYT+EK+AVD++L++RD IHSSFT  +   N  G+GS+ S   + 
Sbjct: 314 TEYRKALARFQEVTNRYTQEKEAVDDMLRERDDIHSSFTTERTMVNSVGAGSSSSRYPT- 372

Query: 411 KISGEDSKGESPGEEGGSDGKDKSGKKKWFNLNLKSSDKR 292
                    ESP E G  DGKDKS KKKWFNLNL  SDK+
Sbjct: 373 ---------ESP-ENGNIDGKDKSSKKKWFNLNLNRSDKK 402

>pir||T00641 hypothetical protein F3I6.4 - Arabidopsis thaliana
           gi|2829865|gb|AAC00573.1| N-terminal region similar to
           DNA-J proteins [Arabidopsis thaliana]
          Length = 388

 Score = 66.6 bits (161), Expect = 2e-10
 Identities = 40/119 (33%), Positives = 61/119 (50%), Gaps = 20/119 (16%)
 Frame = -2

Query: 588 SEYRKALARYQEVTDRYTKEKQAVDELLKQRDGIHSSFTIVKPTNISGSGSNLSNGSSSK 409
           +EYR+ L ++ ++T RY +E Q++DELLKQR+ IHS++T +     S S + +   S  K
Sbjct: 268 TEYREVLVQFTDMTSRYAQEMQSIDELLKQRNEIHSAYTTIPLMKRSSSKNRMRKSSFKK 327

Query: 408 ISG---------EDSKGESPGEEGGSDGKDK-----------SGKKKWFNLNLKSSDKR 292
            +          E+ + E   EE  S  K+K             K KWFNL+LK   K+
Sbjct: 328 AAAKAPAPTEQEEEEEEEEEEEEESSRQKNKKPSTCDKSETLKKKSKWFNLHLKLDKKK 386

>ref|NP_173822.2| DNAJ-like heatshock protein; protein id: At1g24120.1, supported by
           cDNA: gi_18377834 [Arabidopsis thaliana]
           gi|18377835|gb|AAL67104.1| At1g24120/F3I6_4 [Arabidopsis
           thaliana]
          Length = 436

 Score = 66.6 bits (161), Expect = 2e-10
 Identities = 40/119 (33%), Positives = 61/119 (50%), Gaps = 20/119 (16%)
 Frame = -2

Query: 588 SEYRKALARYQEVTDRYTKEKQAVDELLKQRDGIHSSFTIVKPTNISGSGSNLSNGSSSK 409
           +EYR+ L ++ ++T RY +E Q++DELLKQR+ IHS++T +     S S + +   S  K
Sbjct: 316 TEYREVLVQFTDMTSRYAQEMQSIDELLKQRNEIHSAYTTIPLMKRSSSKNRMRKSSFKK 375

Query: 408 ISG---------EDSKGESPGEEGGSDGKDK-----------SGKKKWFNLNLKSSDKR 292
            +          E+ + E   EE  S  K+K             K KWFNL+LK   K+
Sbjct: 376 AAAKAPAPTEQEEEEEEEEEEEEESSRQKNKKPSTCDKSETLKKKSKWFNLHLKLDKKK 434

>dbj|BAC43485.1| unknown protein [Arabidopsis thaliana] gi|28973195|gb|AAO63922.1|
           unknown protein [Arabidopsis thaliana]
          Length = 414

 Score = 58.9 bits (141), Expect = 5e-08
 Identities = 36/101 (35%), Positives = 57/101 (55%), Gaps = 2/101 (1%)
 Frame = -2

Query: 588 SEYRKALARYQEVTDRYTKEKQAVDELLKQRDGIHSSFTIVKPTNISGSGSNLSNGSSSK 409
           +EY +  A++ E+  + T E Q +DELLK+R+ I +++TI  PT     GS+ S   S K
Sbjct: 319 AEYHEVFAQFTEMASKCTGEVQEIDELLKRRNEICAAYTIFPPTK---QGSSKSRSWSKK 375

Query: 408 ISGEDSKGESPGEEGGSDGKDKSG--KKKWFNLNLKSSDKR 292
              + S    P EEG    +++ G  KKKW+N+ L+   K+
Sbjct: 376 ---KSSLLMEPREEGEVAVREEGGVKKKKWYNIQLRQDKKK 413

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 500,564,113
Number of Sequences: 1393205
Number of extensions: 11065645
Number of successful extensions: 46323
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 40582
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 45713
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22283372436
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf080f11 BP073290 1 523 2 MR011d05_f BP076783 79 247 3 GNf036b03 BP069960 79 532 4 GNf089a04 BP073906 79 556 5 GNf047h08 BP070873 79 580 6 GENf063g11 BP061061 93 452 7 GENf034c08 BP059793 95 560 8 GNf100b11 BP074760 96 494 9 MR043e05_f BP079333 96 487 10 GNf031f03 BP069631 96 506 11 SPD061a09_f BP048819 96 527 12 SPD041c02_f BP047243 97 555 13 GENf084d07 BP061924 97 429 14 MR023a08_f BP077723 100 488 15 MFB016b10_f BP035085 123 669

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