Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002049A_C02 KMC002049A_c02
(655 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T12317 26S proteasome regulatory subunit S5A - common ice p... 132 4e-30
pir||T51606 probable 26S proteasome non-ATPase chain S5a [import... 106 3e-22
gb|AAF74210.1|AF076610_1 multiubiquitin chain-binding protein [P... 89 4e-17
ref|NP_195575.1| 26S proteasome regulatory subunit S5A (RPN10); ... 89 5e-17
gb|AAM65985.1| multiubiquitin chain binding protein MBP1 [Arabid... 89 5e-17
>pir||T12317 26S proteasome regulatory subunit S5A - common ice plant
gi|3202042|gb|AAC19402.1| 26S proteasome regulatory
subunit S5A [Mesembryanthemum crystallinum]
Length = 405
Score = 132 bits (332), Expect = 4e-30
Identities = 70/98 (71%), Positives = 81/98 (82%), Gaps = 3/98 (3%)
Frame = -1
Query: 655 DMMDDENALLQQALAMSMDDPVINKDMRDTDMSEAAADDPELALALQLSVADGSEDSGSQ 476
D+ DDENALLQQALAMSMD+P +RDTDM++A DD +L LALQLSV D +++S SQ
Sbjct: 287 DLTDDENALLQQALAMSMDEPASTTTVRDTDMADATGDDQDLQLALQLSVQDSAKESTSQ 346
Query: 475 S---DMSKLLADQSFVSSILASLPGVDPNDPSVKDLLA 371
S ++ KLLADQSFVSSILASLPGVDPNDPSVKDLLA
Sbjct: 347 SNPEEVGKLLADQSFVSSILASLPGVDPNDPSVKDLLA 384
Score = 36.6 bits (83), Expect = 0.30
Identities = 21/42 (50%), Positives = 26/42 (61%)
Frame = -2
Query: 426 LHRFPGLTQMTHLSKIYWPAMQNQSEPQQKNEDKPPNEEEKK 301
L PG+ K +MQNQSE +K+EDKPP+EEEKK
Sbjct: 366 LASLPGVDPNDPSVKDLLASMQNQSE--KKDEDKPPSEEEKK 405
>pir||T51606 probable 26S proteasome non-ATPase chain S5a [imported] - rice
gi|3550985|dbj|BAA32704.1| OsS5a [Oryza sativa (japonica
cultivar-group)] gi|17297981|dbj|BAB78488.1| 26S
proteasome regulatory particle non-ATPase subunit10
[Oryza sativa (japonica cultivar-group)]
Length = 402
Score = 106 bits (264), Expect = 3e-22
Identities = 59/92 (64%), Positives = 68/92 (73%)
Frame = -1
Query: 646 DDENALLQQALAMSMDDPVINKDMRDTDMSEAAADDPELALALQLSVADGSEDSGSQSDM 467
DD+ LLQQALAMSM++ D M+EAA DD +LALALQ+SV D SQSDM
Sbjct: 290 DDDAQLLQQALAMSMEEGSSGAAAADAAMAEAAVDDQDLALALQMSVQDAG--GSSQSDM 347
Query: 466 SKLLADQSFVSSILASLPGVDPNDPSVKDLLA 371
SK+ D+SFV+SIL SLPGVDPNDPSVKDLLA
Sbjct: 348 SKVFEDRSFVTSILNSLPGVDPNDPSVKDLLA 379
>gb|AAF74210.1|AF076610_1 multiubiquitin chain-binding protein [Physcomitrella patens]
Length = 414
Score = 89.4 bits (220), Expect = 4e-17
Identities = 53/105 (50%), Positives = 70/105 (66%), Gaps = 11/105 (10%)
Frame = -1
Query: 652 MMDDENALLQQALAMSMDDPVI-------NKDMRDTDMSEAAADDPELALALQLSV---- 506
M+DD+ ALLQQA+AMSM DT M E+ DD +LA ALQ+S+
Sbjct: 292 MIDDDTALLQQAIAMSMAQAAQAAAAAGGGPATTDTSMEESGGDDQDLAYALQMSMQQAA 351
Query: 505 ADGSEDSGSQSDMSKLLADQSFVSSILASLPGVDPNDPSVKDLLA 371
A S GS +D++++L DQSFVSS+L+SLPGVDPNDP+V+D+LA
Sbjct: 352 AAASAAQGS-ADVNQVLGDQSFVSSVLSSLPGVDPNDPNVRDVLA 395
>ref|NP_195575.1| 26S proteasome regulatory subunit S5A (RPN10); protein id:
At4g38630.1, supported by cDNA: 6757., supported by
cDNA: gi_1165205, supported by cDNA: gi_13430813,
supported by cDNA: gi_21281019 [Arabidopsis thaliana]
gi|1709794|sp|P55034|PSD4_ARATH 26S proteasome
non-ATPase regulatory subunit 4 (26S proteasome
regulatory subunit S5A) (Multiubiquitin chain binding
protein) gi|7487961|pir||T05691 multiubiquitin
chain-binding protein MBP1 - Arabidopsis thaliana
gi|1165206|gb|AAA85583.1| MBP1
gi|4467150|emb|CAB37519.1| multiubiquitin chain binding
protein (MBP1) [Arabidopsis thaliana]
gi|7270846|emb|CAB80527.1| multiubiquitin chain binding
protein (MBP1) [Arabidopsis thaliana]
gi|13430814|gb|AAK26029.1|AF360319_1 putative
multiubiquitin chain binding protein MBP1 [Arabidopsis
thaliana] gi|21281020|gb|AAM44937.1| putative
multiubiquitin chain binding protein MBP1 [Arabidopsis
thaliana]
Length = 386
Score = 89.0 bits (219), Expect = 5e-17
Identities = 49/93 (52%), Positives = 69/93 (73%)
Frame = -1
Query: 649 MDDENALLQQALAMSMDDPVINKDMRDTDMSEAAADDPELALALQLSVADGSEDSGSQSD 470
MD+++ALL QA+AMS+ D +MSEAA +D +LALALQ+S++ G E S +
Sbjct: 280 MDEDSALLDQAIAMSVGD---------VNMSEAADEDQDLALALQMSMS-GEESSEATGA 329
Query: 469 MSKLLADQSFVSSILASLPGVDPNDPSVKDLLA 371
+ LL +Q+F+SS+L+SLPGVDPNDP+VK+LLA
Sbjct: 330 GNNLLGNQAFISSVLSSLPGVDPNDPAVKELLA 362
>gb|AAM65985.1| multiubiquitin chain binding protein MBP1 [Arabidopsis thaliana]
Length = 386
Score = 89.0 bits (219), Expect = 5e-17
Identities = 49/93 (52%), Positives = 69/93 (73%)
Frame = -1
Query: 649 MDDENALLQQALAMSMDDPVINKDMRDTDMSEAAADDPELALALQLSVADGSEDSGSQSD 470
MD+++ALL QA+AMS+ D +MSEAA +D +LALALQ+S++ G E S +
Sbjct: 280 MDEDSALLDQAIAMSVGD---------VNMSEAADEDQDLALALQMSMS-GEESSEATGA 329
Query: 469 MSKLLADQSFVSSILASLPGVDPNDPSVKDLLA 371
+ LL +Q+F+SS+L+SLPGVDPNDP+VK+LLA
Sbjct: 330 GNNLLGNQAFISSVLSSLPGVDPNDPAVKELLA 362
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 551,053,692
Number of Sequences: 1393205
Number of extensions: 11796905
Number of successful extensions: 32490
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 31043
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32417
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 28144814643
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)