>KMC002039A_C01 KMC002039A_c01
aaatCAATGAAGAATCATTATACTTTCAGTTATCTTACTAATAATCCCTGGCAACAGCCA
GAAAGGGGGGCAATAGCATGGAATTTATCAAACATGAACTGAAAAGAGTCTCAATTATTT
TGGAAAAGCATGATGCCAGTCTCAAGCAGCAGCTATTTTACACAAAGCTAAACTTTCAAT
CAGGCCAACGAGGAAGGGAATCACAAAGAATAGTTCCGTATCGAAACAACAATATCTCTC
ACCTTCTAAGTTTTTCTCTATTCTTTTTTTTTTTGTACTATTTATCTATCACCTGAAATC
TTTTAGCAAGTCCAAGAGGATGATCTTTTAATCACTGGTTTCATCTGCTTGTCTTCTTCA
AGGGAAATCATCCCCAAGTGTGAGGGGTCCTCCAGCATCATCTTTGCCACCAGATAACCT
GCAATAGACCATGTTTGGTACTTTCTTGCTTGCTTGCCTACATATCTCCCAAGTTTACCA
TCATAATATTCAGGCCACCCGTCCTTCAATAGACGACTCTCAGCAAGCTCAATTGCACGT
CTTGCTATCTGTGGTCGCCCCGTTTTGATGCAAGCAGCCGTTACAAGCCACAGCAGCACT
GGCCAAGATCCTCCATTATGGTAACTCCATCTGGTATTTTTGGGATCACAACCAGTAACT
ATTCGCCACTCATGACTTTCTATTGCAGGATAAGATATTTTTAGTGGCATTTCTCCTACC
AATTCATCCCAGCGAGCTTCAATAAGATCCATGATAGCCATTGATTGCTCTGGGGTTGCT
AAAGATGATAGAATTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002039A_C01 KMC002039A_c01
         (796 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_567347.1| neutral invertase like protein; protein id: At4...   314  8e-85
pir||A85097 neutral invertase like protein [imported] - Arabidop...   314  8e-85
ref|NP_195212.1| invertase - like protein; protein id: At4g34860...   311  5e-84
ref|NP_564177.1| putative invertase; protein id: At1g22650.1, su...   310  2e-83
gb|AAM65926.1| putative invertase [Arabidopsis thaliana]              307  1e-82

>ref|NP_567347.1| neutral invertase like protein; protein id: At4g09510.1, supported
           by cDNA: 156092. [Arabidopsis thaliana]
          Length = 558

 Score =  314 bits (805), Expect = 8e-85
 Identities = 147/163 (90%), Positives = 155/163 (94%)
 Frame = -3

Query: 794 ILSSLATPEQSMAIMDLIEARWDELVGEMPLKISYPAIESHEWRIVTGCDPKNTRWSYHN 615
           ILSSLATP+QSMAIMDL+E RW+ELVGEMPLKI YP IESHEWRIVTGCDPKNTRWSYHN
Sbjct: 396 ILSSLATPDQSMAIMDLLEHRWEELVGEMPLKICYPCIESHEWRIVTGCDPKNTRWSYHN 455

Query: 614 GGSWPVLLWLVTAACIKTGRPQIARRAIELAESRLLKDGWPEYYDGKLGRYVGKQARKYQ 435
           GGSWPVLLW +TAACIKTGRPQIARRAI+L ESRL +D WPEYYDGK GRYVGKQARKYQ
Sbjct: 456 GGSWPVLLWTLTAACIKTGRPQIARRAIDLIESRLHRDCWPEYYDGKQGRYVGKQARKYQ 515

Query: 434 TWSIAGYLVAKMMLEDPSHLGMISLEEDKQMKPVIKRSSSWTC 306
           TWSIAGYLVAKMMLEDPSH+GMISLEEDKQMKPVIKRS+SWTC
Sbjct: 516 TWSIAGYLVAKMMLEDPSHIGMISLEEDKQMKPVIKRSASWTC 558

>pir||A85097 neutral invertase like protein [imported] - Arabidopsis thaliana
           gi|7267646|emb|CAB78074.1| neutral invertase like
           protein [Arabidopsis thaliana]
          Length = 566

 Score =  314 bits (805), Expect = 8e-85
 Identities = 147/163 (90%), Positives = 155/163 (94%)
 Frame = -3

Query: 794 ILSSLATPEQSMAIMDLIEARWDELVGEMPLKISYPAIESHEWRIVTGCDPKNTRWSYHN 615
           ILSSLATP+QSMAIMDL+E RW+ELVGEMPLKI YP IESHEWRIVTGCDPKNTRWSYHN
Sbjct: 404 ILSSLATPDQSMAIMDLLEHRWEELVGEMPLKICYPCIESHEWRIVTGCDPKNTRWSYHN 463

Query: 614 GGSWPVLLWLVTAACIKTGRPQIARRAIELAESRLLKDGWPEYYDGKLGRYVGKQARKYQ 435
           GGSWPVLLW +TAACIKTGRPQIARRAI+L ESRL +D WPEYYDGK GRYVGKQARKYQ
Sbjct: 464 GGSWPVLLWTLTAACIKTGRPQIARRAIDLIESRLHRDCWPEYYDGKQGRYVGKQARKYQ 523

Query: 434 TWSIAGYLVAKMMLEDPSHLGMISLEEDKQMKPVIKRSSSWTC 306
           TWSIAGYLVAKMMLEDPSH+GMISLEEDKQMKPVIKRS+SWTC
Sbjct: 524 TWSIAGYLVAKMMLEDPSHIGMISLEEDKQMKPVIKRSASWTC 566

>ref|NP_195212.1| invertase - like protein; protein id: At4g34860.1, supported by
           cDNA: gi_15215775 [Arabidopsis thaliana]
           gi|7486858|pir||T10232 hypothetical protein T11I11.100 -
           Arabidopsis thaliana gi|5123703|emb|CAB45447.1|
           invertase-like protein [Arabidopsis thaliana]
           gi|7270437|emb|CAB80203.1| invertase-like protein
           [Arabidopsis thaliana] gi|15215776|gb|AAK91433.1|
           AT4g34860/F11I11_100 [Arabidopsis thaliana]
           gi|27363384|gb|AAO11611.1| At4g34860/F11I11_100
           [Arabidopsis thaliana]
          Length = 571

 Score =  311 bits (798), Expect = 5e-84
 Identities = 143/163 (87%), Positives = 157/163 (95%)
 Frame = -3

Query: 794 ILSSLATPEQSMAIMDLIEARWDELVGEMPLKISYPAIESHEWRIVTGCDPKNTRWSYHN 615
           ILSSLATPEQS AIMDLIE+RW+ELVGEMPLK+ YPAIESHEWRIVTGCDPKNTRWSYHN
Sbjct: 409 ILSSLATPEQSTAIMDLIESRWEELVGEMPLKVCYPAIESHEWRIVTGCDPKNTRWSYHN 468

Query: 614 GGSWPVLLWLVTAACIKTGRPQIARRAIELAESRLLKDGWPEYYDGKLGRYVGKQARKYQ 435
           GGSWPVLLWL+TAACIKTGRPQIARRAIE+AE+RL KD WPEYYDGK+GRYVGKQ+RK Q
Sbjct: 469 GGSWPVLLWLLTAACIKTGRPQIARRAIEVAEARLHKDHWPEYYDGKVGRYVGKQSRKNQ 528

Query: 434 TWSIAGYLVAKMMLEDPSHLGMISLEEDKQMKPVIKRSSSWTC 306
           TWS+AGYLVAKMMLEDPSH+GM+ LEEDKQMKPV++RS+SWTC
Sbjct: 529 TWSVAGYLVAKMMLEDPSHVGMVCLEEDKQMKPVMRRSNSWTC 571

>ref|NP_564177.1| putative invertase; protein id: At1g22650.1, supported by cDNA:
           6399. [Arabidopsis thaliana]
          Length = 534

 Score =  310 bits (793), Expect = 2e-83
 Identities = 144/162 (88%), Positives = 156/162 (95%)
 Frame = -3

Query: 794 ILSSLATPEQSMAIMDLIEARWDELVGEMPLKISYPAIESHEWRIVTGCDPKNTRWSYHN 615
           IL+SLATPEQS +IMDLIE RW+ELVGEMP+KI +PAIESHEWRIVTGCDPKNTRWSYHN
Sbjct: 373 ILASLATPEQSASIMDLIEERWEELVGEMPVKICHPAIESHEWRIVTGCDPKNTRWSYHN 432

Query: 614 GGSWPVLLWLVTAACIKTGRPQIARRAIELAESRLLKDGWPEYYDGKLGRYVGKQARKYQ 435
           GGSWPVLLWL+TAACIKTGRPQIARRAI+LAE+RLLKDGWPEYYDGK GR++GKQARK+Q
Sbjct: 433 GGSWPVLLWLLTAACIKTGRPQIARRAIDLAEARLLKDGWPEYYDGKSGRFIGKQARKFQ 492

Query: 434 TWSIAGYLVAKMMLEDPSHLGMISLEEDKQMKPVIKRSSSWT 309
           TWSIAGYLVAKM+LEDPSHLGMISLEEDKQ KPVIKRS SWT
Sbjct: 493 TWSIAGYLVAKMLLEDPSHLGMISLEEDKQTKPVIKRSYSWT 534

>gb|AAM65926.1| putative invertase [Arabidopsis thaliana]
          Length = 534

 Score =  307 bits (787), Expect = 1e-82
 Identities = 143/162 (88%), Positives = 155/162 (95%)
 Frame = -3

Query: 794 ILSSLATPEQSMAIMDLIEARWDELVGEMPLKISYPAIESHEWRIVTGCDPKNTRWSYHN 615
           IL+SLATPEQS +IMDLIE RW+ELVG MP+KI +PAIESHEWRIVTGCDPKNTRWSYHN
Sbjct: 373 ILASLATPEQSASIMDLIEERWEELVGAMPVKICHPAIESHEWRIVTGCDPKNTRWSYHN 432

Query: 614 GGSWPVLLWLVTAACIKTGRPQIARRAIELAESRLLKDGWPEYYDGKLGRYVGKQARKYQ 435
           GGSWPVLLWL+TAACIKTGRPQIARRAI+LAE+RLLKDGWPEYYDGK GR++GKQARK+Q
Sbjct: 433 GGSWPVLLWLLTAACIKTGRPQIARRAIDLAEARLLKDGWPEYYDGKSGRFIGKQARKFQ 492

Query: 434 TWSIAGYLVAKMMLEDPSHLGMISLEEDKQMKPVIKRSSSWT 309
           TWSIAGYLVAKM+LEDPSHLGMISLEEDKQ KPVIKRS SWT
Sbjct: 493 TWSIAGYLVAKMLLEDPSHLGMISLEEDKQTKPVIKRSYSWT 534

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 683,582,531
Number of Sequences: 1393205
Number of extensions: 15137029
Number of successful extensions: 40844
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 39408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40804
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 40055936206
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL064g10_f BP055999 1 88 2 MR025f01_f BP077924 5 396 3 MWM184a11_f AV767555 96 428 4 SPD049h11_f BP047949 244 590 5 GENf032h12 BP059731 275 635 6 MWM067b05_f AV765777 275 728 7 MFBL044g04_f BP043516 279 793 8 MFB057h12_f BP038163 280 797 9 MFB053d07_f BP037840 281 760 10 MR090e08_f BP082933 287 741

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