>KMC001999A_C04 KMC001999A_c04
tgggtcgggcccccctcagtttttttttttttttttGAAAGAAAGAAAAGTTGCTTTTAT
CTGAATAATAAAACCTGACCCATGCGAAAATGAGGGAGAAAATTTATGGTATTTGAAGAC
TGAGGAAATATCCAATAAACTGAGAACTTGAAAAACAAAAAAGTACGAAGATAATAGAAT
GTTCCTCAAGTCTGGCAGGAAACAACACAAGCATCAAAAATATAATTACTAGTTCCTAGG
TGTTTAAAACATGATCACCCCATGACACCAAAACTGCAAGCAAACTCAAGCCTCCACCCT
TTCCTCCTCAAGGATAAGGGCCTTGGCTGTGTGGCTTAGCCAAAAGATCGGTATACTCTG
GGAATGGAGATTGGGTGAAACGAGTCTCCTTCCAGAATTCAGGTGTCAGAAAACCATAGG
TCTTCAGACAGACAATCAAAATGTAGCCTTGACGAAATTGCCAAGGGTTTGGGTGGAACC
CCTGGAGGAAGTGAAAACATCATCAATCCCAGCGAACTGCAAGACCTTCTTGGGAACCCT
AGCAGCAACGATTCCAGAACCACGAGGAGCAGGGACCATGCGGACAGTGACGGAACCACA
CTTTCCGGTAACCTTGCAGGGAACGGTGTGGGGCTTTCCGATCTTGTTACCCCAGTATCC
CCTCCTGACGGGGATAACAGAGAGCTTAGCAAGGATAATCCCACCGCGAATGGCGGTAGC
AACTTCCTTGCTGCACTTCACGCCGAGCCCGACGTGACCATTGTTGTCGCCGACGACGAC
GAAGGCCTTGAACCTGGTTCGCTGACCGGCACGGGTCTGCTTCTGGACCGGCATGATCTT
CATGACCTCGTCCTTGAGAGTGGGTCCAACCAACATGTCGATGATCTGGTGCTCCTTGAT
TGGGAGGGAGTGGAGGTAGATCTGCTCCAGGCTTCGGATTTTGCCGTCTCTGACAAGACG
ACCTAGCTTGGTCACGGGGACCCACTTCTCCTCCTCGTCGCGCCTGCCACCAGTCCTGCG
GCGTCCGCCACGGCCTCTGTCTCCACGGCCTCTGTCGCCACGACCGCCGAAGCCACGGCC
GAATCCACCACGATCTCCGCCACCACGTTCAGCCATTTTGGTTTTGTTGTGAGGGAACGG
CGAGAATTCACt

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001999A_C04 KMC001999A_c04
         (1152 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_181715.1| 40S ribosomal protein S2; protein id: At2g41840...   402  e-121
gb|AAM62944.1| 40S ribosomal protein S2 [Arabidopsis thaliana]        400  e-120
ref|NP_191308.1| 40S ribosomal protein S2 homolog; protein id: A...   396  e-119
gb|AAM67061.1| ribosomal protein S2, putative [Arabidopsis thali...   395  e-118
ref|NP_176134.1| ribosomal protein S2, putative; protein id: At1...   395  e-118

>ref|NP_181715.1| 40S ribosomal protein S2; protein id: At2g41840.1, supported by cDNA:
            17415., supported by cDNA: gi_15081714 [Arabidopsis
            thaliana] gi|3915847|sp|P49688|RS2_ARATH 40S ribosomal
            protein S2 gi|25294522|pir||G84846 40S ribosomal protein
            S2 [imported] - Arabidopsis thaliana
            gi|2335095|gb|AAC02764.1| 40S ribosomal protein S2
            [Arabidopsis thaliana] gi|15081715|gb|AAK82512.1|
            At2g41840/T11A7.6 [Arabidopsis thaliana]
            gi|22137092|gb|AAM91391.1| At2g41840/T11A7.6 [Arabidopsis
            thaliana]
          Length = 285

 Score =  402 bits (1034), Expect(2) = e-121
 Identities = 205/235 (87%), Positives = 213/235 (90%), Gaps = 9/235 (3%)
 Frame = -1

Query: 1116 MAERGG-------GDRGGFGRGFGGRGDRG-RGDRGRGGRR-RTGGRRDEEEKWVPVTKL 964
            MAERGG       G+RG FGRGFGGRG RG RG RGRGGR  R GGR  EEEKWVPVTKL
Sbjct: 1    MAERGGERGVERGGERGDFGRGFGGRGGRGDRGGRGRGGRGGRRGGRASEEEKWVPVTKL 60

Query: 963  GRLVRDGKIRSLEQIYLHSLPIKEHQIIDMLVGPTLKDEVMKIMPVQKQTRAGQRTRFKA 784
            GRLV  G I+ +EQIYLHSLP+KE+QIIDML+GPTLKDEVMKIMPVQKQTRAGQRTRFKA
Sbjct: 61   GRLVAAGHIKQIEQIYLHSLPVKEYQIIDMLIGPTLKDEVMKIMPVQKQTRAGQRTRFKA 120

Query: 783  FVVVGDNNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTG 604
            FVVVGD NGHVGLGVKCSKEVATAIRG IILAKLSV+PVRRGYWGNKIGKPHTVPCKVTG
Sbjct: 121  FVVVGDGNGHVGLGVKCSKEVATAIRGAIILAKLSVVPVRRGYWGNKIGKPHTVPCKVTG 180

Query: 603  KCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTQTLGNFVKATF 439
            KCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGST+TLGNFVKATF
Sbjct: 181  KCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATF 235

 Score = 56.6 bits (135), Expect(2) = e-121
 Identities = 25/32 (78%), Positives = 29/32 (90%), Gaps = 1/32 (3%)
 Frame = -3

Query: 430 CL-KTYGFLTPEFWKETRFTQSPFPEYTDLLA 338
           CL KTYGFLTPEFWKETRF++SP+ E+TD LA
Sbjct: 237 CLQKTYGFLTPEFWKETRFSRSPYQEHTDFLA 268

>gb|AAM62944.1| 40S ribosomal protein S2 [Arabidopsis thaliana]
          Length = 285

 Score =  400 bits (1027), Expect(2) = e-120
 Identities = 204/235 (86%), Positives = 212/235 (89%), Gaps = 9/235 (3%)
 Frame = -1

Query: 1116 MAERGG-------GDRGGFGRGFGGRGDRG-RGDRGRGGRR-RTGGRRDEEEKWVPVTKL 964
            MAERGG       G+RG FGRGFGGRG RG RG RGRGGR  R GGR  EEEKWVPVTKL
Sbjct: 1    MAERGGERGVERGGERGDFGRGFGGRGGRGDRGGRGRGGRGGRRGGRASEEEKWVPVTKL 60

Query: 963  GRLVRDGKIRSLEQIYLHSLPIKEHQIIDMLVGPTLKDEVMKIMPVQKQTRAGQRTRFKA 784
            GR V  G I+ +EQIYLHSLP+KE+QIIDML+GPTLKDEVMKIMPVQKQTRAGQRTRFKA
Sbjct: 61   GRHVAAGHIKQIEQIYLHSLPVKEYQIIDMLIGPTLKDEVMKIMPVQKQTRAGQRTRFKA 120

Query: 783  FVVVGDNNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTG 604
            FVVVGD NGHVGLGVKCSKEVATAIRG IILAKLSV+PVRRGYWGNKIGKPHTVPCKVTG
Sbjct: 121  FVVVGDGNGHVGLGVKCSKEVATAIRGAIILAKLSVVPVRRGYWGNKIGKPHTVPCKVTG 180

Query: 603  KCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTQTLGNFVKATF 439
            KCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGST+TLGNFVKATF
Sbjct: 181  KCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATF 235

 Score = 56.6 bits (135), Expect(2) = e-120
 Identities = 25/32 (78%), Positives = 29/32 (90%), Gaps = 1/32 (3%)
 Frame = -3

Query: 430 CL-KTYGFLTPEFWKETRFTQSPFPEYTDLLA 338
           CL KTYGFLTPEFWKETRF++SP+ E+TD LA
Sbjct: 237 CLQKTYGFLTPEFWKETRFSRSPYQEHTDFLA 268

>ref|NP_191308.1| 40S ribosomal protein S2 homolog; protein id: At3g57490.1, supported
            by cDNA: 101250. [Arabidopsis thaliana]
            gi|11276536|pir||T46185 ribosomal protein S2, cytosolic
            [similarity] - Arabidopsis thaliana
            gi|6706420|emb|CAB66106.1| 40S ribosomal protein S2
            homolog [Arabidopsis thaliana] gi|21536514|gb|AAM60846.1|
            40S ribosomal protein S2 homolog [Arabidopsis thaliana]
          Length = 276

 Score =  396 bits (1017), Expect(2) = e-119
 Identities = 198/229 (86%), Positives = 210/229 (91%), Gaps = 3/229 (1%)
 Frame = -1

Query: 1116 MAERG---GGDRGGFGRGFGGRGDRGRGDRGRGGRRRTGGRRDEEEKWVPVTKLGRLVRD 946
            MAERG   GGDRG FGRGFGGRG    G RGRG   R  GR  EEEKWVPVTKLGRLV++
Sbjct: 1    MAERGVERGGDRGDFGRGFGGRGGGRGGPRGRG---RRAGRAPEEEKWVPVTKLGRLVKE 57

Query: 945  GKIRSLEQIYLHSLPIKEHQIIDMLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAFVVVGD 766
            GKI  +EQIYLHSLP+KE+QIID+LVGP+LKDEVMKIMPVQKQTRAGQRTRFKAF+VVGD
Sbjct: 58   GKITKIEQIYLHSLPVKEYQIIDLLVGPSLKDEVMKIMPVQKQTRAGQRTRFKAFIVVGD 117

Query: 765  NNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTGKCGSVT 586
            +NGHVGLGVKCSKEVATAIRG IILAKLSV+P+RRGYWGNKIGKPHTVPCKVTGKCGSVT
Sbjct: 118  SNGHVGLGVKCSKEVATAIRGAIILAKLSVVPIRRGYWGNKIGKPHTVPCKVTGKCGSVT 177

Query: 585  VRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTQTLGNFVKATF 439
            VRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGST+TLGNFVKATF
Sbjct: 178  VRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATF 226

 Score = 56.2 bits (134), Expect(2) = e-119
 Identities = 25/34 (73%), Positives = 29/34 (84%), Gaps = 1/34 (2%)
 Frame = -3

Query: 430 CL-KTYGFLTPEFWKETRFTQSPFPEYTDLLAKP 332
           CL KTYGFLTPEFWKETRF++SP+ E+TD L  P
Sbjct: 228 CLQKTYGFLTPEFWKETRFSKSPYQEHTDFLLIP 261

>gb|AAM67061.1| ribosomal protein S2, putative [Arabidopsis thaliana]
          Length = 284

 Score =  395 bits (1015), Expect(2) = e-118
 Identities = 203/234 (86%), Positives = 207/234 (87%), Gaps = 8/234 (3%)
 Frame = -1

Query: 1116 MAERGG-------GDRGGFGRGFGGRGDRGRG-DRGRGGRRRTGGRRDEEEKWVPVTKLG 961
            MAERGG       GDRG FGRGFGG    GRG DRG  GR R GGR  EE KWVPVTKLG
Sbjct: 1    MAERGGEGGTERGGDRGDFGRGFGGGRGGGRGRDRGPRGRGRRGGRASEETKWVPVTKLG 60

Query: 960  RLVRDGKIRSLEQIYLHSLPIKEHQIIDMLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAF 781
            RLV D KI  LEQIYLHSLP+KE+QIID LVGPTLKDEVMKIMPVQKQTRAGQRTRFKAF
Sbjct: 61   RLVADNKITKLEQIYLHSLPVKEYQIIDHLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAF 120

Query: 780  VVVGDNNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTGK 601
            VVVGD NGHVGLGVKCSKEVATAIRG IILAKLSV+PVRRGYWGNKIGKPHTVPCKVTGK
Sbjct: 121  VVVGDGNGHVGLGVKCSKEVATAIRGAIILAKLSVVPVRRGYWGNKIGKPHTVPCKVTGK 180

Query: 600  CGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTQTLGNFVKATF 439
            CGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGST+TLGNFVKATF
Sbjct: 181  CGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATF 234

 Score = 55.5 bits (132), Expect(2) = e-118
 Identities = 24/32 (75%), Positives = 29/32 (90%), Gaps = 1/32 (3%)
 Frame = -3

Query: 430 CL-KTYGFLTPEFWKETRFTQSPFPEYTDLLA 338
           CL KTYGFLTPEFWKETRF++SP+ E+TD L+
Sbjct: 236 CLQKTYGFLTPEFWKETRFSRSPYQEHTDFLS 267

>ref|NP_176134.1| ribosomal protein S2, putative; protein id: At1g58380.1 [Arabidopsis
            thaliana] gi|11276533|pir||T50673 ribosomal protein S2
            homolog XW6 [imported] - Arabidopsis thaliana
            gi|6566272|dbj|BAA88263.1| similar to ribosomal protein
            S2 [Arabidopsis thaliana]
            gi|12321043|gb|AAG50639.1|AC082643_3 ribosomal protein
            S2, putative [Arabidopsis thaliana]
            gi|14423442|gb|AAK62403.1|AF386958_1 ribosomal protein
            S2, putative [Arabidopsis thaliana]
            gi|18181932|dbj|BAB83870.1| ribosomal protein S2
            [Arabidopsis thaliana] gi|18377554|gb|AAL66943.1|
            ribosomal protein S2, putative [Arabidopsis thaliana]
          Length = 284

 Score =  395 bits (1015), Expect(2) = e-118
 Identities = 203/234 (86%), Positives = 207/234 (87%), Gaps = 8/234 (3%)
 Frame = -1

Query: 1116 MAERGG-------GDRGGFGRGFGGRGDRGRG-DRGRGGRRRTGGRRDEEEKWVPVTKLG 961
            MAERGG       GDRG FGRGFGG    GRG DRG  GR R GGR  EE KWVPVTKLG
Sbjct: 1    MAERGGEGGAERGGDRGDFGRGFGGGRGGGRGRDRGPRGRGRRGGRASEETKWVPVTKLG 60

Query: 960  RLVRDGKIRSLEQIYLHSLPIKEHQIIDMLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAF 781
            RLV D KI  LEQIYLHSLP+KE+QIID LVGPTLKDEVMKIMPVQKQTRAGQRTRFKAF
Sbjct: 61   RLVADNKITKLEQIYLHSLPVKEYQIIDHLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAF 120

Query: 780  VVVGDNNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTGK 601
            VVVGD NGHVGLGVKCSKEVATAIRG IILAKLSV+PVRRGYWGNKIGKPHTVPCKVTGK
Sbjct: 121  VVVGDGNGHVGLGVKCSKEVATAIRGAIILAKLSVVPVRRGYWGNKIGKPHTVPCKVTGK 180

Query: 600  CGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTQTLGNFVKATF 439
            CGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGST+TLGNFVKATF
Sbjct: 181  CGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATF 234

 Score = 55.5 bits (132), Expect(2) = e-118
 Identities = 24/32 (75%), Positives = 29/32 (90%), Gaps = 1/32 (3%)
 Frame = -3

Query: 430 CL-KTYGFLTPEFWKETRFTQSPFPEYTDLLA 338
           CL KTYGFLTPEFWKETRF++SP+ E+TD L+
Sbjct: 236 CLQKTYGFLTPEFWKETRFSRSPYQEHTDFLS 267

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,186,474,887
Number of Sequences: 1393205
Number of extensions: 32869057
Number of successful extensions: 286587
Number of sequences better than 10.0: 2519
Number of HSP's better than 10.0 without gapping: 132303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 234914
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 70830964476
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD026b07_f BP046033 1 585 2 MF084c05_f BP032717 15 519 3 SPD084b10_f BP050688 60 669 4 MFB058d09_f BP038203 61 663 5 MPD003e07_f AV770199 68 660 6 MPD062e02_f AV774141 77 634 7 SPD077e06_f BP050166 78 718 8 SPD069c11_f BP049509 78 660 9 SPD091g09_f BP051297 81 464 10 MFB054b12_f BP037904 84 701 11 SPD076c01_f BP050059 84 690 12 SPD076b06_f BP050054 84 660 13 MF015f10_f BP029050 84 591 14 MF056d06_f BP031239 85 592 15 MFB078h07_f BP039735 86 639 16 MPD008f12_f AV770546 87 621 17 MF063b08_f BP031624 87 541 18 MPD012h06_f AV770848 87 636 19 SPD097c11_f BP051740 88 677 20 SPD029h01_f BP046333 88 747 21 MPDL038f01_f AV778435 88 661 22 MPD080e06_f AV775260 90 610 23 MF076h03_f BP032344 92 619 24 GNf091a09 BP074056 92 645 25 GNf051c09 BP071138 92 497 26 GNf096f10 BP074499 92 468 27 GENf029g05 BP059592 92 327 28 SPD054e08_f BP048316 92 658 29 SPD057g01_f BP048559 92 680 30 SPD088b12_f BP051012 92 678 31 MFB076g06_f BP039577 92 709 32 MFB065e04_f BP038720 92 623 33 SPD092c11_f BP051334 92 557 34 SPD025g02_f BP045998 92 544 35 MF019b09_f BP029240 92 525 36 SPD075e01_f BP049996 92 639 37 MR034c10_f BP078613 92 271 38 MFB038h11_f BP036825 92 660 39 MFB026f05_f BP035904 92 629 40 SPD093a08_f BP051392 92 662 41 MFB092c12_f BP040712 92 609 42 MF015e11_f BP029042 92 591 43 MF030a03_f BP029827 92 593 44 MFB062h04_f BP038530 92 620 45 MF012c08_f BP028858 93 658 46 SPD096h08_f BP051710 93 600 47 SPD013b02_f BP045007 93 638 48 SPD050a07_f BP047954 93 638 49 SPD014a02_f BP045077 93 638 50 MFB047g01_f BP037439 94 662 51 MPDL084e03_f AV780875 94 238 52 MF021c01_f BP029362 94 554 53 GNf093g02 BP074268 95 501 54 SPD083e12_f BP050639 95 632 55 MF070c03_f BP031999 95 615 56 MF096h11_f BP033325 96 636 57 SPD022a03_f BP045691 96 660 58 MR092f07_f BP083097 96 493 59 MPDL019f05_f AV777474 100 579 60 MF058e12_f BP031364 101 581 61 GNf003b07 BP067571 102 575 62 GNf025d03 BP069168 102 638 63 GNf073b07 BP072750 106 533 64 GENf074b07 BP061514 106 486 65 GNf099f05 BP074718 106 631 66 MR070e11_f BP081395 106 638 67 SPD076d12_f BP050080 106 717 68 MF060a09_f BP031441 107 538 69 SPD074h12_f BP049951 107 698 70 MFB060b02_f BP038327 108 639 71 MFB041d02_f BP036977 108 701 72 SPD031c08_f BP046451 108 542 73 SPD019c07_f BP045481 108 717 74 SPD034f09_f BP046724 108 696 75 MFB087b11_f BP040342 108 596 76 SPD043f07_f BP047444 108 662 77 MR056c02_f BP080291 108 527 78 SPD013f07_f BP045049 111 683 79 MFB059f12_f BP038297 111 506 80 SPD072g06_f BP049783 111 622 81 GNLf009d10 BP075336 113 616 82 MFB056b03_f BP038033 113 744 83 SPD042d11_f BP047346 113 658 84 MF029c04_f BP029786 114 535 85 GNf098g02 BP074652 117 638 86 MF074g10_f BP032248 117 669 87 MFB025f12_f BP035827 117 665 88 SPD096c06_f BP051659 120 629 89 GNf083a09 BP073461 120 240 90 GNf082h07 BP073451 120 555 91 SPD072h07_f BP049796 120 407 92 SPD075a06_f BP049957 120 661 93 SPD022a02_f BP045690 120 589 94 MF027b01_f BP029669 120 629 95 SPD070e05_f BP049606 122 597 96 MF087b11_f BP032863 123 662 97 GNf003b10 BP067573 125 661 98 SPD028g09_f BP046247 127 743 99 MWM124a02_f AV766705 129 485 100 GNf100d05 BP074776 131 631 101 SPD075b08_f BP049970 131 625 102 SPD008c12_f BP044628 132 466 103 MF031a01_f BP029884 152 578 104 MPDL035g03_f AV778259 153 642 105 SPD022c08_f BP045719 155 730 106 MFB002b05_f BP034017 161 771 107 MWM134e05_f AV766845 205 726 108 SPD033f08_f BP046640 684 1174 109 MF034h07_f BP030120 691 1177 110 MF027g07_f BP029705 752 1178 111 MWM132g10_f AV766825 880 1175 112 MPDL068h03_f AV779996 1084 1164

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