KMC001997A_c02
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC001997A_C02 KMC001997A_c02
atcaaaatcattaataTGCAATTATAAGTACGATAATGGCCGGCTATGCAATTCTTATTT
TAATGAGTAAATCATTACTCGGAATTATGTCTACCTTTGCATTTATGCAGGTAATAACAA
CTCAAAACATCCAAAGTAAAATCAGCAGAAATGTAGCAACTCAGGTTCAAAATTACAAAA
GACTAATCAGTTCAAACAAATATGGGGAAGCAGCAAAGCCCTAGTATTCCCACCCAACAC
ACCAAGTGTAGGGAAAAGGGGTTAAGGACAGATGCTTCTTTTCCCTTTCTTATTGTCTTG
TGACAGTGCACACGATCCCTTTTCAGGCTTTGCTCTATCGTCTCCTGTCTGAAATGACTC
TAAACCCATCATTTTAAGCAGAAAGCTTTGCCTTGAGCTCGGCTGCCACAGCATCGATGA
ATTCCTCAGTGTTCAAATAATGCTCCCTTGAAAGCTTGGATCCATGAAGAATAAGTGCCA
AATCCTTGGTCATCTTCCCTGACTCTACTACTCCGATGCAAGCTGCCTCTAGCTTGTCAG
TGAAATCCGAGAGTTTAGCATTGTCATCCAGTTTAGCCCTGTGAGCTAGCCCTCTTGTCC
AAGCAAAGATGGATGCTATGCTGTTTGTGCTGGTTTCCCCACCCTTCTGATGAACCCTAA
AATGACGGGTAACTGTGCCATGGGCTGCTTCTGCTTCAATGGTCTTTCCATCAGGGCAAA
CCAGTACAGATGTCATCAAACCCAATGATCCAAAACCTTGAGCTAAGAAATCACTCTGGA
CATCTCCATCGTAGTTTTTGCATGCCCATACATAACCTCCTT
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001997A_C02 KMC001997A_c02
(822 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||S33612 isocitrate dehydrogenase (NADP) (EC 1.1.1.42) - soybean 291 1e-77
sp|Q06197|IDHC_SOYBN ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCI... 291 1e-77
gb|AAA33978.1| NADPH-specific isocitrate dehydrogenase 291 1e-77
sp|Q40345|IDHP_MEDSA ISOCITRATE DEHYDROGENASE [NADP], CHLOROPLAS... 290 1e-77
ref|NP_176768.1| isocitrate dehydrogenase, putative; protein id:... 283 2e-75
>pir||S33612 isocitrate dehydrogenase (NADP) (EC 1.1.1.42) - soybean
Length = 451
Score = 291 bits (744), Expect = 1e-77
Identities = 143/148 (96%), Positives = 146/148 (98%)
Frame = -3
Query: 820 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 641
GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG
Sbjct: 304 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 363
Query: 640 GETSTNSIASIFAWTRGLAHRAKLDDNAKLSDFTDKLEAACIGVVESGKMTKDLALILHG 461
GETSTNSIASIFAWTRGLAHRAKLDDNAKL DFT+KLEAACIGVVE+GKMTKDLALILHG
Sbjct: 364 GETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLEAACIGVVEAGKMTKDLALILHG 423
Query: 460 SKLSREHYLNTEEFIDAVAAELKAKLSA 377
SKLSREHYLNTEEFIDAVAAEL A+LSA
Sbjct: 424 SKLSREHYLNTEEFIDAVAAELSARLSA 451
>sp|Q06197|IDHC_SOYBN ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
Length = 413
Score = 291 bits (744), Expect = 1e-77
Identities = 143/148 (96%), Positives = 146/148 (98%)
Frame = -3
Query: 820 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 641
GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG
Sbjct: 266 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 325
Query: 640 GETSTNSIASIFAWTRGLAHRAKLDDNAKLSDFTDKLEAACIGVVESGKMTKDLALILHG 461
GETSTNSIASIFAWTRGLAHRAKLDDNAKL DFT+KLEAACIGVVE+GKMTKDLALILHG
Sbjct: 326 GETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLEAACIGVVEAGKMTKDLALILHG 385
Query: 460 SKLSREHYLNTEEFIDAVAAELKAKLSA 377
SKLSREHYLNTEEFIDAVAAEL A+LSA
Sbjct: 386 SKLSREHYLNTEEFIDAVAAELSARLSA 413
>gb|AAA33978.1| NADPH-specific isocitrate dehydrogenase
Length = 441
Score = 291 bits (744), Expect = 1e-77
Identities = 143/148 (96%), Positives = 146/148 (98%)
Frame = -3
Query: 820 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 641
GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG
Sbjct: 294 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 353
Query: 640 GETSTNSIASIFAWTRGLAHRAKLDDNAKLSDFTDKLEAACIGVVESGKMTKDLALILHG 461
GETSTNSIASIFAWTRGLAHRAKLDDNAKL DFT+KLEAACIGVVE+GKMTKDLALILHG
Sbjct: 354 GETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLEAACIGVVEAGKMTKDLALILHG 413
Query: 460 SKLSREHYLNTEEFIDAVAAELKAKLSA 377
SKLSREHYLNTEEFIDAVAAEL A+LSA
Sbjct: 414 SKLSREHYLNTEEFIDAVAAELSARLSA 441
>sp|Q40345|IDHP_MEDSA ISOCITRATE DEHYDROGENASE [NADP], CHLOROPLAST PRECURSOR
(OXALOSUCCINATE DECARBOXYLASE) (IDH) (NADP+-SPECIFIC
ICDH) (IDP) gi|7488742|pir||T09619 isocitrate
dehydrogenase (NADP) (EC 1.1.1.42) precursor - alfalfa
(fragment) gi|166386|gb|AAA32656.1| isocitrate
dehydrogenase
Length = 433
Score = 290 bits (743), Expect = 1e-77
Identities = 143/148 (96%), Positives = 145/148 (97%)
Frame = -3
Query: 820 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 641
GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG
Sbjct: 286 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 345
Query: 640 GETSTNSIASIFAWTRGLAHRAKLDDNAKLSDFTDKLEAACIGVVESGKMTKDLALILHG 461
GETSTNSIASIFAWTRGLAHRAKLDDNA L DFT+KLEAACIGVVESGKMTKDLALILHG
Sbjct: 346 GETSTNSIASIFAWTRGLAHRAKLDDNATLLDFTEKLEAACIGVVESGKMTKDLALILHG 405
Query: 460 SKLSREHYLNTEEFIDAVAAELKAKLSA 377
SKLSREHYLNTEEFIDAVAAELK K+SA
Sbjct: 406 SKLSREHYLNTEEFIDAVAAELKTKISA 433
>ref|NP_176768.1| isocitrate dehydrogenase, putative; protein id: At1g65930.1,
supported by cDNA: 41544., supported by cDNA:
gi_15010659, supported by cDNA: gi_16930442, supported
by cDNA: gi_20260383, supported by cDNA: gi_20453234
[Arabidopsis thaliana] gi|25283700|pir||F96683
hypothetical protein F12P19.10 [imported] - Arabidopsis
thaliana gi|6227018|gb|AAF06054.1|AC009513_10 Strong
similarity to gb|AF155333 NADP-specific isocitrate
dehydrogenase from Oryza sativa. ESTs gb|R30474,
gb|H36712, gb|T22563, gb|N97293, gb|T43729, gb|Z17440,
gb|Z34193, gb|Z46528, gb|T14072, gb|T42413, gb|AA389759,
gb|N38098, gb|T43337, gb|N96032, gb|N96031 and gb|Z38038
come from this gene. [Arabidopsis thaliana]
gi|15010660|gb|AAK73989.1| At1g65930/F12P19_10
[Arabidopsis thaliana]
gi|16930443|gb|AAL31907.1|AF419575_1 At1g65930/F12P19_10
[Arabidopsis thaliana] gi|20453235|gb|AAM19856.1|
At1g65930/F12P19_10 [Arabidopsis thaliana]
gi|27311589|gb|AAO00760.1| isocitrate dehydrogenase,
putative [Arabidopsis thaliana]
Length = 410
Score = 283 bits (724), Expect = 2e-75
Identities = 138/148 (93%), Positives = 144/148 (97%)
Frame = -3
Query: 820 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 641
GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG
Sbjct: 263 GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKG 322
Query: 640 GETSTNSIASIFAWTRGLAHRAKLDDNAKLSDFTDKLEAACIGVVESGKMTKDLALILHG 461
GETSTNSIASIFAWTRGLAHRAKLDDNAKL DFT+KLEAAC+G VESGKMTKDLALI+HG
Sbjct: 323 GETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLEAACVGTVESGKMTKDLALIIHG 382
Query: 460 SKLSREHYLNTEEFIDAVAAELKAKLSA 377
SKLSR+ YLNTEEFIDAVAAELK +L+A
Sbjct: 383 SKLSRDTYLNTEEFIDAVAAELKERLNA 410
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 699,227,744
Number of Sequences: 1393205
Number of extensions: 15313940
Number of successful extensions: 33171
Number of sequences better than 10.0: 219
Number of HSP's better than 10.0 without gapping: 31910
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33089
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 42576939184
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
MF027c10_f |
BP029682 |
1 |
327 |
| 2 |
MR038d12_f |
BP078946 |
4 |
165 |
| 3 |
MPDL058g02_f |
AV779464 |
11 |
535 |
| 4 |
GNf006f01 |
BP067819 |
29 |
455 |
| 5 |
MR025f04_f |
BP077927 |
30 |
541 |
| 6 |
MF069f11_f |
BP031979 |
30 |
137 |
| 7 |
MFB004b11_f |
BP034167 |
31 |
555 |
| 8 |
MFBL008g12_f |
BP041690 |
31 |
541 |
| 9 |
SPDL033c05_f |
BP054046 |
34 |
439 |
| 10 |
GNf032c02 |
BP069678 |
39 |
509 |
| 11 |
MF035a02_f |
BP030125 |
40 |
566 |
| 12 |
GNf034e05 |
BP069845 |
41 |
469 |
| 13 |
MR043g08_f |
BP079355 |
41 |
421 |
| 14 |
MFB069f06_f |
BP039027 |
43 |
148 |
| 15 |
MFB022b03_f |
BP035553 |
43 |
212 |
| 16 |
MPD033a12_f |
AV772234 |
48 |
629 |
| 17 |
MR058h12_f |
BP080492 |
48 |
398 |
| 18 |
MF043a01_f |
BP030528 |
53 |
136 |
| 19 |
GENf049d08 |
BP060419 |
59 |
571 |
| 20 |
MR010a03_f |
BP076672 |
59 |
535 |
| 21 |
MR067b07_f |
BP081131 |
59 |
543 |
| 22 |
MFBL028f03_f |
BP042671 |
59 |
630 |
| 23 |
MPD005f12_f |
AV770347 |
62 |
598 |
| 24 |
SPD089a08_f |
BP051079 |
65 |
437 |
| 25 |
MFB065g06_f |
BP038741 |
71 |
224 |
| 26 |
MFB013e07_f |
BP034879 |
77 |
581 |
| 27 |
GNf012a10 |
BP068212 |
80 |
391 |
| 28 |
SPDL025a10_f |
BP053527 |
80 |
589 |
| 29 |
GNf077h01 |
BP073090 |
80 |
490 |
| 30 |
SPD026c02_f |
BP046040 |
80 |
647 |
| 31 |
GENf029e08 |
BP059583 |
80 |
449 |
| 32 |
GNf012e06 |
BP068247 |
81 |
489 |
| 33 |
MF067h04_f |
BP031890 |
81 |
597 |
| 34 |
MR096h11_f |
BP083396 |
454 |
832 |
|
Lotus japonicus
Kazusa DNA Research Institute