Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001972A_C01 KMC001972A_c01
(527 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_196455.1| glycosyl hydrolase family 27 (alpha-galactosida... 250 6e-66
gb|AAM13199.1| alpha-galactosidase-like protein [Arabidopsis tha... 248 2e-65
pir||T10860 alpha-galactosidase (EC 3.2.1.22) - kidney bean gi|9... 241 5e-63
sp|Q42656|AGAL_COFAR ALPHA-GALACTOSIDASE PRECURSOR (MELIBIASE) (... 241 5e-63
pir||T06388 alpha-galactosidase (EC 3.2.1.22) - soybean gi|92757... 238 4e-62
>ref|NP_196455.1| glycosyl hydrolase family 27 (alpha-galactosidase/melibiase);
protein id: At5g08380.1, supported by cDNA: gi_20260601
[Arabidopsis thaliana] gi|10178280|emb|CAC08338.1|
alpha-galactosidase-like protein [Arabidopsis thaliana]
Length = 410
Score = 250 bits (639), Expect = 6e-66
Identities = 117/152 (76%), Positives = 132/152 (85%)
Frame = +3
Query: 72 MCVTASSSGPKNLNNIEEQFRRNLLANGLGRTPPMGWNSWNHFNCQINEKIIRETADALI 251
M + SS N ++ E RR+LL NGLG TPPMGWNSWNHF+C I+EK+I+ETADAL+
Sbjct: 22 MTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALV 81
Query: 252 STGLSKLGYNYVNIDDCWAELNRDHKGNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDA 431
+TGLSKLGYNYVNIDDCWAE++RD KG+LVPKKSTFPSGIKA+ADYVHSKGLKLGIYSDA
Sbjct: 82 TTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDA 141
Query: 432 GYFTCSKKMPGSLGHEFQDAKTFASWGIDYLK 527
GYFTCSK MPGSLG+E DAKTFA WGIDYLK
Sbjct: 142 GYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLK 173
>gb|AAM13199.1| alpha-galactosidase-like protein [Arabidopsis thaliana]
Length = 410
Score = 248 bits (634), Expect = 2e-65
Identities = 116/152 (76%), Positives = 132/152 (86%)
Frame = +3
Query: 72 MCVTASSSGPKNLNNIEEQFRRNLLANGLGRTPPMGWNSWNHFNCQINEKIIRETADALI 251
M + SS N ++ E RR+LL NGLG TPPMGWNSWNHF+C I+EK+I+ETA+AL+
Sbjct: 22 MTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETANALV 81
Query: 252 STGLSKLGYNYVNIDDCWAELNRDHKGNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDA 431
+TGLSKLGYNYVNIDDCWAE++RD KG+LVPKKSTFPSGIKA+ADYVHSKGLKLGIYSDA
Sbjct: 82 TTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDA 141
Query: 432 GYFTCSKKMPGSLGHEFQDAKTFASWGIDYLK 527
GYFTCSK MPGSLG+E DAKTFA WGIDYLK
Sbjct: 142 GYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLK 173
>pir||T10860 alpha-galactosidase (EC 3.2.1.22) - kidney bean
gi|927577|gb|AAA73964.1| alpha-galactosidase
Length = 425
Score = 241 bits (614), Expect = 5e-63
Identities = 110/136 (80%), Positives = 120/136 (87%)
Frame = +3
Query: 120 EEQFRRNLLANGLGRTPPMGWNSWNHFNCQINEKIIRETADALISTGLSKLGYNYVNIDD 299
E RRNL+ NGLG+TPPMGWNSWNHF+C INE +IRETADA++STGL+ LGY Y+NIDD
Sbjct: 55 EVDHRRNLVGNGLGQTPPMGWNSWNHFSCNINEDLIRETADAMVSTGLAALGYQYINIDD 114
Query: 300 CWAELNRDHKGNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDAGYFTCSKKMPGSLGHE 479
CW ELNRD +GNLVPK STFPSG+KALADYVH KGLKLGIYSDAG TCSK MPGSLGHE
Sbjct: 115 CWGELNRDSQGNLVPKASTFPSGMKALADYVHKKGLKLGIYSDAGTQTCSKTMPGSLGHE 174
Query: 480 FQDAKTFASWGIDYLK 527
QDAKTFASWGIDYLK
Sbjct: 175 EQDAKTFASWGIDYLK 190
>sp|Q42656|AGAL_COFAR ALPHA-GALACTOSIDASE PRECURSOR (MELIBIASE) (ALPHA-D-GALACTOSIDE
GALACTOHYDROLASE) gi|11264279|pir||T50781
alpha-galactosidase (EC 3.2.1.22) [imported] - coffee
gi|504489|gb|AAA33022.1| alpha-galactosidase
Length = 378
Score = 241 bits (614), Expect = 5e-63
Identities = 110/136 (80%), Positives = 122/136 (88%)
Frame = +3
Query: 120 EEQFRRNLLANGLGRTPPMGWNSWNHFNCQINEKIIRETADALISTGLSKLGYNYVNIDD 299
E+ RR+LLANGLG TPPMGWNSWNHF C ++EK+IRETADA++S GL+ LGY Y+N+DD
Sbjct: 8 EDYTRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDD 67
Query: 300 CWAELNRDHKGNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDAGYFTCSKKMPGSLGHE 479
CWAELNRD +GNLVPK STFPSGIKALADYVHSKGLKLGIYSDAG TCSK MPGSLGHE
Sbjct: 68 CWAELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHE 127
Query: 480 FQDAKTFASWGIDYLK 527
QDAKTFASWG+DYLK
Sbjct: 128 EQDAKTFASWGVDYLK 143
>pir||T06388 alpha-galactosidase (EC 3.2.1.22) - soybean
gi|927575|gb|AAA73963.1| alpha galactosidase
Length = 422
Score = 238 bits (606), Expect = 4e-62
Identities = 118/164 (71%), Positives = 127/164 (76%), Gaps = 9/164 (5%)
Frame = +3
Query: 63 LVGMCVTASSSGPKNLNNI---------EEQFRRNLLANGLGRTPPMGWNSWNHFNCQIN 215
LV + ASSS + L N +E RRNLL NGLG TPPMGWNSWNHF C I
Sbjct: 24 LVMLSNNASSSSARLLFNRTRGGFTIMPKEVHRRNLLDNGLGHTPPMGWNSWNHFACNIK 83
Query: 216 EKIIRETADALISTGLSKLGYNYVNIDDCWAELNRDHKGNLVPKKSTFPSGIKALADYVH 395
E +IRETADA++STGL+ LGY Y+NIDDCW ELNRD KGNLVPK STFPSG+KALADYVH
Sbjct: 84 EDLIRETADAMVSTGLAALGYQYINIDDCWGELNRDSKGNLVPKASTFPSGMKALADYVH 143
Query: 396 SKGLKLGIYSDAGYFTCSKKMPGSLGHEFQDAKTFASWGIDYLK 527
GLKLGIYSDAG TCSK MPGSLGHE QDAKTFASWGIDYLK
Sbjct: 144 KNGLKLGIYSDAGNQTCSKTMPGSLGHEEQDAKTFASWGIDYLK 187
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 449,847,887
Number of Sequences: 1393205
Number of extensions: 9346720
Number of successful extensions: 30542
Number of sequences better than 10.0: 119
Number of HSP's better than 10.0 without gapping: 27048
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29692
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17308240320
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
