KMC001920A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC001920A_C01 KMC001920A_c01
gttgttgGCACGAAAACATAATACTATATATAGCACAAACTGGTATTTGCAGAGTACAAA
ATGCTGCAAAGCTTACAACATTACCACACAGAAAAGACATCCAAAGCTGTTGAAAAATAC
AAGTGATGACTCTTCTCTGAAATATTGAGAAGTTCATTTTTTTAAACCTCTCAGCAATTG
CTGTCACTGTCTGTCTTGTATCACACACTGTATATAAGTTGTAAACTGGAGAAGAAAGAG
GATGAGCACTCCCTTCTTGTCTCTTCACTCCCTTCCGCCCCGGTCCTTCCATCTTATATA
ATCTACCTATTTTTATCTAACCTAATGAAATGCAAAATACACAAGAGCAGAAGAAGATTT
GACACCAGAAATGGAAAAGGAAGATAAAATAACCTCTCGCACTGCACATTTTGTATGGCT
ACACAGTCTTACACACACCAGTGGACCGTTCCCTCGTGACTTAAACGGTTAAACCAACTT
GATCATTCTCAGGCCAATAAGTACTTGGCGACTGAGACATCTGCTGGAGAATTAGCAGCA
TGGATGTAGACAAATTCAAGGCTTTTACCAGCTGATAATGAACTGATCCATGAAGGG


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001920A_C01 KMC001920A_c01
         (597 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAG31636.1| endo-beta-1,4-D-glucanase [Lycopersicon esculentum]     54  1e-06
pir||T09873 probable cellulase (EC 3.2.1.4) - upland cotton (fra...    53  3e-06
dbj|BAC22691.1| endo-1,4-beta-D-glucanase [Pyrus communis]             49  6e-05
emb|CAC94006.1| endo-beta-1,4-glucanase [Fragaria x ananassa]          48  8e-05
gb|AAC78298.2| cellulase [Fragaria x ananassa]                         48  8e-05

>gb|AAG31636.1| endo-beta-1,4-D-glucanase [Lycopersicon esculentum]
          Length = 43

 Score = 54.3 bits (129), Expect = 1e-06
 Identities = 25/34 (73%), Positives = 28/34 (81%)
 Frame = -2

Query: 596 PSWISSLSAGKSLEFVYIHAANSPADVSVAKYLL 495
           P+WI+SL  GKSLEFVYIH ANSPA VSV+ Y L
Sbjct: 9   PAWINSLPTGKSLEFVYIHTANSPAVVSVSSYTL 42

>pir||T09873 probable cellulase (EC 3.2.1.4) - upland cotton (fragment)
           gi|2244740|dbj|BAA21111.1| endo-1,4-beta-glucanase
           [Gossypium hirsutum]
          Length = 324

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 26/35 (74%), Positives = 30/35 (85%)
 Frame = -2

Query: 596 PSWISSLSAGKSLEFVYIHAANSPADVSVAKYLLA 492
           P W++SL AGKSLEFVYIHAA SPADVSV+ Y +A
Sbjct: 291 PEWLNSLPAGKSLEFVYIHAA-SPADVSVSSYNIA 324

>dbj|BAC22691.1| endo-1,4-beta-D-glucanase [Pyrus communis]
          Length = 620

 Score = 48.5 bits (114), Expect = 6e-05
 Identities = 24/35 (68%), Positives = 29/35 (82%)
 Frame = -2

Query: 596 PSWISSLSAGKSLEFVYIHAANSPADVSVAKYLLA 492
           PSWI+SL  GKS+EFVYIH+A SPA+V V+ Y LA
Sbjct: 587 PSWINSLPVGKSMEFVYIHSA-SPANVLVSSYSLA 620

>emb|CAC94006.1| endo-beta-1,4-glucanase [Fragaria x ananassa]
          Length = 620

 Score = 48.1 bits (113), Expect = 8e-05
 Identities = 25/35 (71%), Positives = 29/35 (82%)
 Frame = -2

Query: 596 PSWISSLSAGKSLEFVYIHAANSPADVSVAKYLLA 492
           PSW++SL AGKSLEFVYIHAA S A+V V+ Y LA
Sbjct: 587 PSWLNSLPAGKSLEFVYIHAA-SAANVLVSSYSLA 620

>gb|AAC78298.2| cellulase [Fragaria x ananassa]
          Length = 620

 Score = 48.1 bits (113), Expect = 8e-05
 Identities = 25/35 (71%), Positives = 29/35 (82%)
 Frame = -2

Query: 596 PSWISSLSAGKSLEFVYIHAANSPADVSVAKYLLA 492
           PSW++SL AGKSLEFVYIHAA S A+V V+ Y LA
Sbjct: 587 PSWLNSLPAGKSLEFVYIHAA-SAANVLVSSYSLA 620

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 499,109,396
Number of Sequences: 1393205
Number of extensions: 10511086
Number of successful extensions: 24683
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 23985
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24677
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23140425222
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MPD017g01_f AV771189 1 533
2 MF063a07_f BP031616 6 343
3 MFBL013c01_f BP041903 8 292
4 MWL013e09_f AV768802 8 603
5 MWM141f10_f AV766935 8 406
6 MRL007a08_f BP084039 8 428
7 MF015f12_f BP029051 8 445
8 MFBL020e08_f BP042271 8 521
9 MRL028h08_f BP085147 9 510
10 SPD095h05_f BP051629 9 560
11 MFB067a02_f BP038827 11 149
12 MF015d02_f BP029031 11 459
13 SPDL079c08_f BP056902 36 613
14 GENf024g01 BP059365 51 428




Lotus japonicus
Kazusa DNA Research Institute