>KMC001897A_C01 KMC001897A_c01
aatAAAAAGAGTATATTCTCATTAATGATTCAGAAGTTGGTAGTACAAGGTAATGACTTT
CCCTTTTATAGGGGGTTTGTCAAGACATGGACTTTTCCTACATTTAGGCCTGACAACTAG
GGCCCAAATCCCTATGCACATAAGACAAATCCAGAAGAATCTTCCAGCTGGCTTGTGGGT
TCGCCTAAAAGGAAGGGCGAGAATCCTAAATGTTTCACCTGTTCCCGCCCTGTCTTCCTT
CATCTGGCTGACTCCTTGTTTTGGGCGAGCATGGAACTCTTTATGTCCCGCCCAGTCCAA
TAATGAAGAAGAAATCAGCTTCTTCCATCAATTTCATATCTGATATACAATCTTTGTACC
CTCACTTTGTGGCATAATTAATGTGCAATTGTACAAGCACATTTGTATCTCTCTAATTAC
AAAATTTACAATTATCTTAATATTTTATTTTTTGGTTACAGGGAGGGGCTAAAGCCCCAA
TTATCTTAATATTAACACATGAGACTATGAGAGATATTTTTAAGTGGATGTTTGGACAAT
ATCAATAATTAAATGACCTTTTTATTGATCATCTTGGAGATTTTAAAGACTCCACATGAG
ATTTTCCACATGGTCCTTTCATTCTAATGAAACGCCTGTATTCATCAAAGGTCATGGCTG
TGTAAAGTGCAGGTTTGTCTTCTTTTACCAATTCTTTGGCTGGTTCAATAGGTATGTCAC
CTCTTGGGTTGTAGAAGAAAGCTAGTGAAACTCTCTCTTTATTTGAATTCACTATTACTC
TATGCTCTACACTCTTGTAGATTGCATTGCTTAGGACCTGAATCTGATCACCAATATTGA
CAATAAAGGCATGGGGAACAGGCTTCACAGTGATCCAATTGTCCCCTTTCCGGACCTGAA
GACCAGTAACTTGATCATCAGGTAGTAAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001897A_C01 KMC001897A_c01
         (929 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM61665.1| leucoanthocyanidin dioxygenase-like protein [Arab...   187  3e-46
ref|NP_196179.1| leucoanthocyanidin dioxygenase-like protein; pr...   187  3e-46
gb|AAD52015.1|AF082862_1 unknown [Pisum sativum]                      186  4e-46
ref|NP_187728.1| putative leucoanthocyanidin dioxygenase; protei...   181  1e-44
ref|NP_191156.1| leucoanthocyanidin dioxygenase -like protein; p...   175  1e-42

>gb|AAM61665.1| leucoanthocyanidin dioxygenase-like protein [Arabidopsis thaliana]
          Length = 355

 Score =  187 bits (474), Expect = 3e-46
 Identities = 92/119 (77%), Positives = 100/119 (83%)
 Frame = -2

Query: 928 LLPDDQVTGLQVRKGDNWITVKPVPHAFIVNIGDQIQVLSNAIYKSVEHRVIVNSNKERV 749
           LLPDDQV GLQVRK D WITVKP PHAFIVNIGDQIQ+LSN+ YKSVEHRVIVNS+KERV
Sbjct: 237 LLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERV 296

Query: 748 SLAFFYNPRGDIPIEPAKELVKEDKPALYTAMTFDEYRRFIRMKGPCGKSHVESLKSPR 572
           SLAFFYNP+ DIPI+P +ELV    P LY  MTFD+YR FIR +GP GKSHVES  SPR
Sbjct: 297 SLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVESHISPR 355

>ref|NP_196179.1| leucoanthocyanidin dioxygenase-like protein; protein id:
           At5g05600.1, supported by cDNA: 13012., supported by
           cDNA: gi_14532537 [Arabidopsis thaliana]
           gi|10178137|dbj|BAB11549.1| leucoanthocyanidin
           dioxygenase-like protein [Arabidopsis thaliana]
           gi|14532538|gb|AAK63997.1| AT5g05600/MOP10_14
           [Arabidopsis thaliana] gi|22137300|gb|AAM91495.1|
           AT5g05600/MOP10_14 [Arabidopsis thaliana]
          Length = 371

 Score =  187 bits (474), Expect = 3e-46
 Identities = 92/119 (77%), Positives = 100/119 (83%)
 Frame = -2

Query: 928 LLPDDQVTGLQVRKGDNWITVKPVPHAFIVNIGDQIQVLSNAIYKSVEHRVIVNSNKERV 749
           LLPDDQV GLQVRK D WITVKP PHAFIVNIGDQIQ+LSN+ YKSVEHRVIVNS+KERV
Sbjct: 253 LLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERV 312

Query: 748 SLAFFYNPRGDIPIEPAKELVKEDKPALYTAMTFDEYRRFIRMKGPCGKSHVESLKSPR 572
           SLAFFYNP+ DIPI+P +ELV    P LY  MTFD+YR FIR +GP GKSHVES  SPR
Sbjct: 313 SLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVESHISPR 371

>gb|AAD52015.1|AF082862_1 unknown [Pisum sativum]
          Length = 134

 Score =  186 bits (472), Expect = 4e-46
 Identities = 88/117 (75%), Positives = 105/117 (89%)
 Frame = -2

Query: 928 LLPDDQVTGLQVRKGDNWITVKPVPHAFIVNIGDQIQVLSNAIYKSVEHRVIVNSNKERV 749
           LLPDD V+GLQVRKG++WITVKPVP+AFI+NIGDQIQV+SNAIYKSVEHRVIVN  ++RV
Sbjct: 16  LLPDDFVSGLQVRKGNDWITVKPVPNAFIINIGDQIQVMSNAIYKSVEHRVIVNPTQDRV 75

Query: 748 SLAFFYNPRGDIPIEPAKELVKEDKPALYTAMTFDEYRRFIRMKGPCGKSHVESLKS 578
           SLA FYNP+ D+ I+PAKELV +++PALY  MT+DEYR +IRMKGPCGK+ VESL S
Sbjct: 76  SLAMFYNPKSDLIIQPAKELVTKERPALYPPMTYDEYRLYIRMKGPCGKAQVESLAS 132

>ref|NP_187728.1| putative leucoanthocyanidin dioxygenase; protein id: At3g11180.1
           [Arabidopsis thaliana]
           gi|6016680|gb|AAF01507.1|AC009991_3 putative
           leucoanthocyanidin dioxygenase [Arabidopsis thaliana]
           gi|12321884|gb|AAG50980.1|AC073395_22 leucoanthocyanidin
           dioxygenase, putative; 41415-43854 [Arabidopsis
           thaliana]
          Length = 400

 Score =  181 bits (460), Expect = 1e-44
 Identities = 89/119 (74%), Positives = 98/119 (81%)
 Frame = -2

Query: 928 LLPDDQVTGLQVRKGDNWITVKPVPHAFIVNIGDQIQVLSNAIYKSVEHRVIVNSNKERV 749
           LLPDDQV GLQVR GD WITV P+ HAFIVNIGDQIQ+LSN+ YKSVEHRVIVNS KERV
Sbjct: 282 LLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERV 341

Query: 748 SLAFFYNPRGDIPIEPAKELVKEDKPALYTAMTFDEYRRFIRMKGPCGKSHVESLKSPR 572
           SLAFFYNP+ DIPI+P ++LV    P LY  MTFD+YR FIR +GP GKSHVES  SPR
Sbjct: 342 SLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVESHISPR 400

>ref|NP_191156.1| leucoanthocyanidin dioxygenase -like protein; protein id:
           At3g55970.1 [Arabidopsis thaliana]
           gi|11255840|pir||T49209 leucoanthocyanidin
           dioxygenase-like protein - Arabidopsis thaliana
           gi|7573492|emb|CAB87851.1| leucoanthocyanidin
           dioxygenase-like protein [Arabidopsis thaliana]
           gi|12043537|emb|CAC19787.1| putative leucoanthocyanidin
           dioxygenase [Arabidopsis thaliana]
          Length = 363

 Score =  175 bits (443), Expect = 1e-42
 Identities = 83/120 (69%), Positives = 100/120 (83%), Gaps = 1/120 (0%)
 Frame = -2

Query: 928 LLPDDQVTGLQVRKGDN-WITVKPVPHAFIVNIGDQIQVLSNAIYKSVEHRVIVNSNKER 752
           LLPD+QV  LQVR  D+ WITV+P PHAFIVN+GDQIQ+LSN+IYKSVEHRVIVN   ER
Sbjct: 244 LLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPENER 303

Query: 751 VSLAFFYNPRGDIPIEPAKELVKEDKPALYTAMTFDEYRRFIRMKGPCGKSHVESLKSPR 572
           +SLAFFYNP+G++PIEP KELV  D PALY++ T+D YR+FIR +GP  K H++ LKSPR
Sbjct: 304 LSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQGPRSKCHIDELKSPR 363

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 823,493,601
Number of Sequences: 1393205
Number of extensions: 18733144
Number of successful extensions: 40704
Number of sequences better than 10.0: 635
Number of HSP's better than 10.0 without gapping: 39181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40610
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 51582455952
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR033d08_f BP078543 1 396 2 MR086e12_f BP082626 4 386 3 MWL021e10_f AV768927 5 572 4 MR067a06_f BP081120 370 798 5 GENf023a01 BP059313 408 520 6 SPD084d03_f BP050702 410 933 7 MR044a01_f BP079371 435 900

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