>KMC001818A_C01 KMC001818A_c01
agaaaatatagaataggatgcgcagctaaacaattacacatgttaaaaggatgcacaata
ccaatAGTACAGGACAAACGACTATGTATATGAATACTGAGACTGTCCACAAAGGCAATA
ATGTGGAGGTGGTAAAAACGTTCCTTCTCCACTTGAGATTTTTTTCATTTTCAAGTATTA
CCTCATCAAACCGTCTAGTAAAACAAAGGCCCCATAAACATTAAAAAGATAACAGATAAA
TAATTTAGCAACATGAAGAGATTTATTGTTCGTCGAACTCATGCATGTGATCTAAAAGGT
AGTTGGCAGCCAATTCCTCATTTTTGTTGCAAGCGAAGAACACCTCCAATACAATCGCAC
GATCGAAACCCATTGCTTCAAGACGTTCAATTGCTTGGCGCTCCTCAGGGGTGACAGTTA
TTGCTTGTGGTATGCCACCAGCCAGCTGCCCCAATGGGTTCCCTTCGCCACCTTCCACAG
GCTCATTTATCAAGCGAAGGAAGTCAGCTTGATGATCTCGAATCAATCTCATTAGATGAG
GATTCTGTTTGCCAAGCTCTTGTAGCATAGGCTGCAATATTTGTGGATTAGCCTGCACCA
TAGCTCGCAAGGCTTGGAATTGTTGGCTGTTGCGAAGAAAATCTAAAGAGCCAGCACCAG
CACCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001818A_C01 KMC001818A_c01
         (665 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAB51544.1| RAD23 protein [Lycopersicon esculentum]               236  3e-61
ref|NP_198663.1| DNA repair protein RAD23 homolog; protein id: A...   227  1e-58
ref|NP_186903.1| putative RAD23; protein id: At3g02540.1, suppor...   225  4e-58
gb|AAK59766.1| unknown protein [Arabidopsis thaliana]                 225  5e-58
pir||T14336 RAD23 protein, isoform I - carrot gi|1914683|emb|CAA...   215  5e-55

>emb|CAB51544.1| RAD23 protein [Lycopersicon esculentum]
          Length = 389

 Score =  236 bits (601), Expect = 3e-61
 Identities = 117/131 (89%), Positives = 125/131 (95%)
 Frame = -2

Query: 661 AGAGSLDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNPHLMRLIRDHQADFLRLINE 482
           AGAG+LDFLRNS QFQALRAMVQANPQILQPMLQELGKQNPHLMRLI++HQ DFLRLINE
Sbjct: 260 AGAGNLDFLRNSPQFQALRAMVQANPQILQPMLQELGKQNPHLMRLIQEHQPDFLRLINE 319

Query: 481 PVEGGEGNPLGQLAGGIPQAITVTPEERQAIERLEAMGFDRAIVLEVFFACNKNEELAAN 302
           PVEG EGN LGQ AG IPQA+TVTPEER+AIERLEAMGFDRA+VLEV+FACNKNEELAAN
Sbjct: 320 PVEG-EGNVLGQTAGAIPQAVTVTPEEREAIERLEAMGFDRALVLEVYFACNKNEELAAN 378

Query: 301 YLLDHMHEFDE 269
           YLLDH+HEFDE
Sbjct: 379 YLLDHLHEFDE 389

>ref|NP_198663.1| DNA repair protein RAD23 homolog; protein id: At5g38470.1,
           supported by cDNA: 36697., supported by cDNA:
           gi_14335003, supported by cDNA: gi_16648837, supported
           by cDNA: gi_19548080 [Arabidopsis thaliana]
           gi|9758825|dbj|BAB09359.1| DNA repair protein RAD23
           homolog [Arabidopsis thaliana]
           gi|16648838|gb|AAL25609.1| unknown protein [Arabidopsis
           thaliana] gi|19548081|gb|AAL87405.1| At5g38470/At5g38470
           [Arabidopsis thaliana] gi|21593157|gb|AAM65106.1| DNA
           repair protein RAD23 homolog [Arabidopsis thaliana]
          Length = 378

 Score =  227 bits (578), Expect = 1e-58
 Identities = 113/133 (84%), Positives = 124/133 (92%)
 Frame = -2

Query: 664 GAGAGSLDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNPHLMRLIRDHQADFLRLIN 485
           GAGAG+LDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNP L+RLI++HQADFLRLIN
Sbjct: 247 GAGAGNLDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNPQLVRLIQEHQADFLRLIN 306

Query: 484 EPVEGGEGNPLGQLAGGIPQAITVTPEERQAIERLEAMGFDRAIVLEVFFACNKNEELAA 305
           EPVE GE N + QL   +PQA+TVTPEER+AIERLE MGFDRA+VLEVFFACNKNEELAA
Sbjct: 307 EPVE-GEENVMEQLEAAMPQAVTVTPEEREAIERLEGMGFDRAMVLEVFFACNKNEELAA 365

Query: 304 NYLLDHMHEFDEQ 266
           NYLLDHMHEF++Q
Sbjct: 366 NYLLDHMHEFEDQ 378

>ref|NP_186903.1| putative RAD23; protein id: At3g02540.1, supported by cDNA:
           gi_14517453 [Arabidopsis thaliana]
           gi|6957717|gb|AAF32461.1| putative RAD23 [Arabidopsis
           thaliana] gi|14517454|gb|AAK62617.1| AT3g02540/F16B3_17
           [Arabidopsis thaliana] gi|21360453|gb|AAM47342.1|
           AT3g02540/F16B3_17 [Arabidopsis thaliana]
          Length = 419

 Score =  225 bits (574), Expect = 4e-58
 Identities = 115/134 (85%), Positives = 125/134 (92%), Gaps = 4/134 (2%)
 Frame = -2

Query: 658 GAGSLDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNPHLMRLIRDHQADFLRLINEP 479
           GAG+LDFLRNSQQFQALRAMVQANPQ+LQPMLQELGKQNP+LMRLI+DHQADFLRLINEP
Sbjct: 286 GAGTLDFLRNSQQFQALRAMVQANPQVLQPMLQELGKQNPNLMRLIQDHQADFLRLINEP 345

Query: 478 VEGG--EGNPLGQLAGGI--PQAITVTPEERQAIERLEAMGFDRAIVLEVFFACNKNEEL 311
           VEGG   GN LGQ+A G+  PQAI VT EER+AIERLEAMGF+RA+VLEVFFACNKNEEL
Sbjct: 346 VEGGGESGNLLGQMAAGMPQPQAIQVTHEEREAIERLEAMGFERALVLEVFFACNKNEEL 405

Query: 310 AANYLLDHMHEFDE 269
           AANYLLDHMHEF+E
Sbjct: 406 AANYLLDHMHEFEE 419

>gb|AAK59766.1| unknown protein [Arabidopsis thaliana]
          Length = 378

 Score =  225 bits (573), Expect = 5e-58
 Identities = 112/133 (84%), Positives = 123/133 (92%)
 Frame = -2

Query: 664 GAGAGSLDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNPHLMRLIRDHQADFLRLIN 485
           GAGAG+LDFLRNS QFQALRAMVQANPQILQPMLQELGKQNP L+RLI++HQADFLRLIN
Sbjct: 247 GAGAGNLDFLRNSHQFQALRAMVQANPQILQPMLQELGKQNPQLVRLIQEHQADFLRLIN 306

Query: 484 EPVEGGEGNPLGQLAGGIPQAITVTPEERQAIERLEAMGFDRAIVLEVFFACNKNEELAA 305
           EPVE GE N + QL   +PQA+TVTPEER+AIERLE MGFDRA+VLEVFFACNKNEELAA
Sbjct: 307 EPVE-GEENVMEQLEAAMPQAVTVTPEEREAIERLEGMGFDRAMVLEVFFACNKNEELAA 365

Query: 304 NYLLDHMHEFDEQ 266
           NYLLDHMHEF++Q
Sbjct: 366 NYLLDHMHEFEDQ 378

>pir||T14336 RAD23 protein, isoform I - carrot gi|1914683|emb|CAA72741.1| RAD23,
           isoform I [Daucus carota]
          Length = 382

 Score =  215 bits (547), Expect = 5e-55
 Identities = 108/130 (83%), Positives = 118/130 (90%)
 Frame = -2

Query: 661 AGAGSLDFLRNSQQFQALRAMVQANPQILQPMLQELGKQNPHLMRLIRDHQADFLRLINE 482
           AGAG+LDFLR +QQFQALRAMVQ+NPQILQPMLQELGKQNPHLMRLI++HQADFL+LINE
Sbjct: 258 AGAGNLDFLRTNQQFQALRAMVQSNPQILQPMLQELGKQNPHLMRLIQEHQADFLQLINE 317

Query: 481 PVEGGEGNPLGQLAGGIPQAITVTPEERQAIERLEAMGFDRAIVLEVFFACNKNEELAAN 302
           P+EGGE      L G  PQAI+VTPEER AIERLEAMGFDR +VLEVFFACNKNEELAAN
Sbjct: 318 PMEGGE-----NLLGHGPQAISVTPEERDAIERLEAMGFDRELVLEVFFACNKNEELAAN 372

Query: 301 YLLDHMHEFD 272
           YLLDHMHEF+
Sbjct: 373 YLLDHMHEFE 382

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 585,974,518
Number of Sequences: 1393205
Number of extensions: 12824440
Number of successful extensions: 42942
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 40788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42869
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28855580904
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR061g08_f BP080696 1 398 2 SPD027a03_f BP046103 66 604 3 GENf017f01 BP059090 67 451 4 MPD063d06_f AV774198 67 156 5 SPD057g05_f BP048562 69 610 6 MWM156e11_f AV767143 78 456 7 MWM014a05_f AV764803 86 586 8 MFBL037b10_f BP043106 89 524 9 SPD079e09_f BP050328 90 675 10 SPD029b02_f BP046271 108 681 11 MR001c11_f BP075994 121 524 12 MR029a11_f BP078209 253 581

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