>KMC001766A_C01 KMC001766A_c01
agAAAGAAACCAGGTATATTTCTCATTTAAGGATGTGATCCTTATGTAGATGGGACCAAA
CTGGACACGGGATAAGTCATAAAGTTTGGACAAAGTTCTGAAGTAGCTATAGACTAGCAA
ACATGATCCTCTTCTTTCCTCTAGAAATGGGGGCTGGCTATATAATGGCAACCCAGGCAG
AGTTTCACACAGGATATGTTTAAGAACAATATATTTTACATTGACACTTCTCTTAACCAG
GCTTTCTACATGTTTTCCAGTTAAGGGTTACACATAATTCTGGTAATATATAAAATCTCA
TAAACCAACTACATTTCCAATGCCAAGGGGCTACTCTCTTCCTGTGGCATCACTAGGAAA
AAAACGAACAATTTAGACTAGCTAAAATGTTTGTCTAGCAATAAAGTAATAAATACAACA
AAGATGGAGCAAAGCCAATCTGGCAAACCACTCACGACCAAAACTAAAGACGAATGATGG
GCTGAACAAATGAGTGACATGCATATTTCTTAATCTTCAAGATCAATTTCCTTCACGTAC
CCAAAATCACTTGACAGAAGATATTCTCTCAATCACAGTAAGGTTTGATGTTGAACCTGT
AAAGAAGCCTCTTCTTTTTCAAGGTGGGATTGTCGACAGTGAGCAATAACTTGCCCAGTT
CAGCAACTTTGAAGTGATTGGAAACCACAGGTTCATCACTAGGGGACATCTTCGTAGCCT
TCTGTATAATAACTGTATATCCATCTTTAGCATCAGGCTTGAATTCAGCACTATAGCTAA
CCTCCCAGCCCACCACACGCAGCTCCCAGACAATAATGCATTTCTCATA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001766A_C01 KMC001766A_c01
         (829 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_177361.1| cytosolic factor, putative; protein id: At1g721...   133  4e-30
gb|AAK82462.1| At1g72160/T9N14_8 [Arabidopsis thaliana] gi|22137...   133  4e-30
ref|NP_192655.1| putative protein; protein id: At4g09160.1 [Arab...   119  5e-26
emb|CAB95829.1| hypothetical protein [Cicer arietinum]                110  3e-23
dbj|BAB86220.1| contains ESTs AU093915(E1276),AU162319(E60301)~s...   104  1e-21

>ref|NP_177361.1| cytosolic factor, putative; protein id: At1g72160.1, supported by
           cDNA: gi_15081613 [Arabidopsis thaliana]
           gi|25406150|pir||A96745 probable cytosolic factor
           T9N14.8 [imported] - Arabidopsis thaliana
           gi|12323663|gb|AAG51796.1|AC067754_12 cytosolic factor,
           putative; 19554-17768 [Arabidopsis thaliana]
          Length = 490

 Score =  133 bits (334), Expect = 4e-30
 Identities = 60/83 (72%), Positives = 71/83 (85%)
 Frame = -1

Query: 829 YEKCIIVWELRVVGWEVSYSAEFKPDAKDGYTVIIQKATKMSPSDEPVVSNHFKVAELGK 650
           YEKC +VWE+RV GWEVSY AEF P+ KD YTV+IQK  KM PSDEPV+++ FKV ELGK
Sbjct: 407 YEKCELVWEIRVTGWEVSYKAEFVPEEKDAYTVVIQKPRKMRPSDEPVLTHSFKVNELGK 466

Query: 649 LLLTVDNPTLKKKRLLYRFNIKP 581
           +LLTVDNPT KKK+L+YRFN+KP
Sbjct: 467 VLLTVDNPTSKKKKLVYRFNVKP 489

>gb|AAK82462.1| At1g72160/T9N14_8 [Arabidopsis thaliana] gi|22137076|gb|AAM91383.1|
           At1g72160/T9N14_8 [Arabidopsis thaliana]
          Length = 390

 Score =  133 bits (334), Expect = 4e-30
 Identities = 60/83 (72%), Positives = 71/83 (85%)
 Frame = -1

Query: 829 YEKCIIVWELRVVGWEVSYSAEFKPDAKDGYTVIIQKATKMSPSDEPVVSNHFKVAELGK 650
           YEKC +VWE+RV GWEVSY AEF P+ KD YTV+IQK  KM PSDEPV+++ FKV ELGK
Sbjct: 307 YEKCELVWEIRVTGWEVSYKAEFVPEEKDAYTVVIQKPRKMRPSDEPVLTHSFKVNELGK 366

Query: 649 LLLTVDNPTLKKKRLLYRFNIKP 581
           +LLTVDNPT KKK+L+YRFN+KP
Sbjct: 367 VLLTVDNPTSKKKKLVYRFNVKP 389

>ref|NP_192655.1| putative protein; protein id: At4g09160.1 [Arabidopsis thaliana]
           gi|25407487|pir||H85092 hypothetical protein AT4g09160
           [imported] - Arabidopsis thaliana
           gi|7267559|emb|CAB78040.1| putative protein [Arabidopsis
           thaliana]
          Length = 723

 Score =  119 bits (298), Expect = 5e-26
 Identities = 55/82 (67%), Positives = 67/82 (81%)
 Frame = -1

Query: 826 EKCIIVWELRVVGWEVSYSAEFKPDAKDGYTVIIQKATKMSPSDEPVVSNHFKVAELGKL 647
           +KC IVWE+RVVGWEVSY AEF P+ K+GYTVIIQK  KM+  +E VVS+ FKV E+G++
Sbjct: 638 QKCTIVWEIRVVGWEVSYGAEFVPENKEGYTVIIQKPRKMTAKNELVVSHSFKVGEVGRI 697

Query: 646 LLTVDNPTLKKKRLLYRFNIKP 581
           LLTVDNPT  KK L+YRF +KP
Sbjct: 698 LLTVDNPTSTKKMLIYRFKVKP 719

>emb|CAB95829.1| hypothetical protein [Cicer arietinum]
          Length = 482

 Score =  110 bits (275), Expect = 3e-23
 Identities = 50/82 (60%), Positives = 65/82 (78%)
 Frame = -1

Query: 826 EKCIIVWELRVVGWEVSYSAEFKPDAKDGYTVIIQKATKMSPSDEPVVSNHFKVAELGKL 647
           EK  +VWE+RVVGW+VSY AEF P A+DGYTVI+QK  K++P+DE V++N FK+ E GK+
Sbjct: 398 EKSTLVWEVRVVGWDVSYGAEFVPSAEDGYTVIVQKNRKIAPADETVINNTFKIGEPGKV 457

Query: 646 LLTVDNPTLKKKRLLYRFNIKP 581
           +LT+DN T KKK+LLYR    P
Sbjct: 458 VLTIDNQTSKKKKLLYRSKTIP 479

>dbj|BAB86220.1| contains ESTs AU093915(E1276),AU162319(E60301)~similar to cytosolic
           factor~unknown protein [Oryza sativa (japonica
           cultivar-group)] gi|20804751|dbj|BAB92436.1| P0698A10.6
           [Oryza sativa (japonica cultivar-group)]
          Length = 613

 Score =  104 bits (260), Expect = 1e-21
 Identities = 47/81 (58%), Positives = 61/81 (75%)
 Frame = -1

Query: 826 EKCIIVWELRVVGWEVSYSAEFKPDAKDGYTVIIQKATKMSPSDEPVVSNHFKVAELGKL 647
           E   I WELRV+GWEVSY AEF PDA+ GYTVI+QK  K+  ++EP++   FKV E GK+
Sbjct: 527 ENSTIGWELRVLGWEVSYGAEFTPDAEGGYTVIVQKTRKVPANEEPIMKGSFKVGEPGKI 586

Query: 646 LLTVDNPTLKKKRLLYRFNIK 584
           +LT++NP  KKK+LLYR  +K
Sbjct: 587 VLTINNPASKKKKLLYRSKVK 607

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 706,694,321
Number of Sequences: 1393205
Number of extensions: 15216126
Number of successful extensions: 36384
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 35236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36379
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 42643890261
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD022d03_f BP045726 1 594 2 MPDL064c12_f AV779750 3 490 3 MFB013d02_f BP034864 119 502 4 SPD017h11_f BP045381 151 406 5 SPD008h11_f BP044674 157 670 6 SPD085b11_f BP050767 164 661 7 GNf008b09 BP067934 171 657 8 SPD093e08_f BP051432 198 779 9 GNf029f08 BP069482 199 590 10 SPDL084c11_f BP057253 199 560 11 MR079g03_f BP082104 199 627 12 MR021f11_f BP077615 200 642 13 MPDL049f04_f AV778989 201 739 14 MPD043f04_f AV772940 202 769 15 MPD035b11_f AV772383 203 788 16 GNf051f10 BP071171 203 706 17 SPD041b02_f BP047233 208 708 18 MFB091d01_f BP040642 208 816 19 SPDL082d05_f BP057120 209 657 20 SPD030d03_f BP046376 225 779 21 SPDL044c11_f BP054764 234 788 22 GENf014d03 BP058931 371 871

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