KMC001746A_c02
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC001746A_C02 KMC001746A_c02
gatcaaacgaaaaatgagcaaatgacatGTAACGAAGAGTGATGTGAAAAATTTGAGAAC
ATATAGAATATTATTTGTGGTTTGGTAGAAGATTACATCAATGAGGAGGAATTATTGAAG
AGAATACAACAGTAGAGGTACATCAGAATTTAAGCTATTTTTTCAAAAGGCAAAAAAAGG
GGACAGTGGTTTTATTGAGCAATCTGGGAGAGTGAAAGAAACGCATTCCTCATAATTCAG
ACAAACTGGTTCAAATATTCATCTGCAGTGGTGAATTTGACATCAGGGTAAAGCTCATGA
GCCTCAGCATCCTGGGCAGGGTCAATCTCATACACTGCATCTCCCTTTAACTGCTGTGAG
TGGTACAGAGCCAATAGGTAGTGGTGAGGGAAACCAGATTCCTTGATGTCCTTAAGAACT
TGTTCCTCAGGAACATAGGTTTTCTCCAAAGTCTTCCCAATCTTCTTCTCCCACAAGGCC
ATGATCTCATTTGCTGTCAAATAATTAGCAGGGAGTCTTATGTGGACAGCCTTGTTCAAT
GTTCTGGGATCGTTCGCGGCTAAGACGGTGAAAGTCCCCACATCTGCCTCAGTGATATAA
GCTCCTTTGACAT
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001746A_C02 KMC001746A_c02
(613 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAG31155.1|AF308858_2 isoflavone reductase [Lotus corniculatus] 229 2e-59
sp|P52575|IFR_MEDSA Isoflavone reductase (IFR) (NADPH:isoflavone... 220 1e-56
pir||S17744 2'-hydroxyisoflavone reductase (EC 1.3.1.45) - alfal... 220 1e-56
sp|P52576|IFR_PEA Isoflavone reductase (IFR) (NADPH:isoflavone o... 219 2e-56
gb|AAF86332.1|AF277052_1 isoflavone reductase [Medicago truncatula] 217 8e-56
>gb|AAG31155.1|AF308858_2 isoflavone reductase [Lotus corniculatus]
Length = 118
Score = 229 bits (584), Expect = 2e-59
Identities = 112/114 (98%), Positives = 114/114 (99%)
Frame = -3
Query: 611 VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV 432
VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV
Sbjct: 5 VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV 64
Query: 431 PEEQVLKDIKESGFPHHYLLALYHSQQLKGDAVYEIDPAQDAEAHELYPDVKFT 270
PEEQVLKDIKESGFPH+YLLALYHSQQLKGDAVYEIDPA+DAEAHELYPDVKFT
Sbjct: 65 PEEQVLKDIKESGFPHNYLLALYHSQQLKGDAVYEIDPAKDAEAHELYPDVKFT 118
>sp|P52575|IFR_MEDSA Isoflavone reductase (IFR) (NADPH:isoflavone oxidoreductase)
gi|608533|gb|AAC48976.1| isoflavone reductase
Length = 318
Score = 220 bits (560), Expect = 1e-56
Identities = 105/124 (84%), Positives = 114/124 (91%)
Frame = -3
Query: 611 VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV 432
VKGAY+TEADVGTFT+ AANDP TLNKAVHIRLP NYLT NE++ALWEKKIGKTLEKTYV
Sbjct: 195 VKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPENYLTQNEVIALWEKKIGKTLEKTYV 254
Query: 431 PEEQVLKDIKESGFPHHYLLALYHSQQLKGDAVYEIDPAQDAEAHELYPDVKFTTADEYL 252
EEQVLKDI+ES FPH+YLLALYHSQQ+KGDAVYEIDPA+D EA E YPDV +TTADEYL
Sbjct: 255 SEEQVLKDIQESSFPHNYLLALYHSQQIKGDAVYEIDPAKDIEASEAYPDVTYTTADEYL 314
Query: 251 NQFV 240
NQFV
Sbjct: 315 NQFV 318
>pir||S17744 2'-hydroxyisoflavone reductase (EC 1.3.1.45) - alfalfa
gi|19620|emb|CAA41106.1| isoflavone reductase [Medicago
sativa]
Length = 318
Score = 220 bits (560), Expect = 1e-56
Identities = 105/124 (84%), Positives = 114/124 (91%)
Frame = -3
Query: 611 VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV 432
VKGAY+TEADVGTFT+ AANDP TLNKAVHIRLP NYLT NE++ALWEKKIGKTLEKTYV
Sbjct: 195 VKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPKNYLTQNEVIALWEKKIGKTLEKTYV 254
Query: 431 PEEQVLKDIKESGFPHHYLLALYHSQQLKGDAVYEIDPAQDAEAHELYPDVKFTTADEYL 252
EEQVLKDI+ES FPH+YLLALYHSQQ+KGDAVYEIDPA+D EA E YPDV +TTADEYL
Sbjct: 255 SEEQVLKDIQESSFPHNYLLALYHSQQIKGDAVYEIDPAKDIEASEAYPDVTYTTADEYL 314
Query: 251 NQFV 240
NQFV
Sbjct: 315 NQFV 318
>sp|P52576|IFR_PEA Isoflavone reductase (IFR) (NADPH:isoflavone oxidoreductase)
gi|1084375|pir||S48631 2'-hydroxyisoflavone reductase
(EC 1.3.1.45) - garden pea gi|619253|gb|AAB31368.1|
isoflavone reductase; IFR [Pisum sativum]
Length = 318
Score = 219 bits (559), Expect = 2e-56
Identities = 103/124 (83%), Positives = 116/124 (93%)
Frame = -3
Query: 611 VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV 432
V+GAY+TEADVGT+T+ AANDP TLNKAVHIRLP NYLTANE++ALWEKKIGKTLEKTYV
Sbjct: 195 VRGAYVTEADVGTYTIRAANDPNTLNKAVHIRLPNNYLTANEVIALWEKKIGKTLEKTYV 254
Query: 431 PEEQVLKDIKESGFPHHYLLALYHSQQLKGDAVYEIDPAQDAEAHELYPDVKFTTADEYL 252
EEQVLKDI+ S FPH+YLLALYHSQQ+KGDAVYEIDPA+D EA++ YPDVK+TTADEYL
Sbjct: 255 SEEQVLKDIQTSSFPHNYLLALYHSQQIKGDAVYEIDPAKDVEAYDAYPDVKYTTADEYL 314
Query: 251 NQFV 240
NQFV
Sbjct: 315 NQFV 318
>gb|AAF86332.1|AF277052_1 isoflavone reductase [Medicago truncatula]
Length = 318
Score = 217 bits (553), Expect = 8e-56
Identities = 103/124 (83%), Positives = 113/124 (91%)
Frame = -3
Query: 611 VKGAYITEADVGTFTVLAANDPRTLNKAVHIRLPANYLTANEIMALWEKKIGKTLEKTYV 432
VKGAY+TEADVGTFT+ AANDP TLNKAVHIRLP NYLT NE+++LWEKKIGKTLEKTYV
Sbjct: 195 VKGAYVTEADVGTFTIKAANDPNTLNKAVHIRLPKNYLTQNEVISLWEKKIGKTLEKTYV 254
Query: 431 PEEQVLKDIKESGFPHHYLLALYHSQQLKGDAVYEIDPAQDAEAHELYPDVKFTTADEYL 252
EEQVLKDI+ES FPH+YLLALYHSQQ+KGDAVYEIDP +D EA E YPDV +TTADEYL
Sbjct: 255 SEEQVLKDIQESSFPHNYLLALYHSQQIKGDAVYEIDPTKDIEASEAYPDVTYTTADEYL 314
Query: 251 NQFV 240
NQFV
Sbjct: 315 NQFV 318
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 511,826,274
Number of Sequences: 1393205
Number of extensions: 11389287
Number of successful extensions: 25700
Number of sequences better than 10.0: 71
Number of HSP's better than 10.0 without gapping: 24589
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25600
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24568846532
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
GNf051h09 |
BP071191 |
1 |
438 |
| 2 |
SPD026b09_f |
BP046035 |
12 |
641 |
| 3 |
SPD060e09_f |
BP048778 |
22 |
512 |
| 4 |
MR062f07_f |
BP080769 |
29 |
438 |
| 5 |
GNf072h09 |
BP072735 |
45 |
150 |
| 6 |
MR069b12_f |
BP081283 |
46 |
608 |
| 7 |
MR006g01_f |
BP076421 |
46 |
478 |
| 8 |
GNf042d12 |
BP070462 |
46 |
468 |
| 9 |
MR019d03_f |
BP077427 |
46 |
152 |
| 10 |
GNf069f11 |
BP072499 |
47 |
564 |
| 11 |
GNf049e11 |
BP071004 |
47 |
619 |
| 12 |
GNf057c02 |
BP071599 |
47 |
566 |
| 13 |
MR057f03_f |
BP080392 |
47 |
502 |
| 14 |
MR049h07_f |
BP079825 |
47 |
608 |
| 15 |
GNf015a03 |
BP068423 |
49 |
261 |
| 16 |
GNf065h07 |
BP072226 |
51 |
270 |
| 17 |
GENf044f04 |
BP060216 |
54 |
438 |
| 18 |
MWL073g07_f |
AV769902 |
55 |
649 |
| 19 |
MR026g06_f |
BP078021 |
55 |
566 |
| 20 |
MR028b04_f |
BP078133 |
55 |
489 |
| 21 |
MR085d08_f |
BP082540 |
55 |
471 |
| 22 |
MR013a08_f |
BP076918 |
55 |
461 |
| 23 |
GENf048e09 |
BP060383 |
55 |
433 |
| 24 |
SPD070g09_f |
BP049629 |
55 |
590 |
| 25 |
GENf013a05 |
BP058879 |
56 |
465 |
| 26 |
SPD051b12_f |
BP048051 |
58 |
506 |
| 27 |
MR080c01_f |
BP082141 |
58 |
480 |
| 28 |
GNf040g04 |
BP070338 |
81 |
496 |
| 29 |
GNf070b10 |
BP072531 |
134 |
562 |
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Lotus japonicus
Kazusa DNA Research Institute