>KMC001739A_C01 KMC001739A_c01
aaccagtggaatctagactagtttAAACAAAAAAAAAAGAACATACTAAGCATACTTCAG
AAAAAATGGCCAGGGTTAAATGGCATTGAAATCCTACTTCAGCATTGCAATCATCAATGC
ACGCCTGATACTAAATATAACAGCAAAATTCAATTCAAACATCCAAGAACTGTAAAATTA
AATAACACAACCAGAAATTAAATAACACAACCAGACGAGACCTAAAGGTAAACTAAGCTG
CAGTTATAACTGCATGATGCAGACAGAAGATAACCACACAAACAACTTAATAGATATGAA
GAATAAGCTGTAGCACAAAATGCACTACTGTTGAGCACATTGTACTCGTTGCGTCGATGG
CTCATCATCATCCTCATCTTCATCATATGCCTCACGGCTCCGTTGCTGCTTCCTTCGACG
CATCTCCTCCTCCAAATTGTTGACATCATGCAAAATGGTCTCCTCACAATCATCCAGCTC
CATATCTGCCTTCTTGCTGCTCTTTTGAGGTAATATCTTTTCTAATAACTGGCATTGATC
AGGGGAAATAAAACCAGAGTCTGGGAAATCAACATTAAACTTGATGTAAAGTCGTCCCTT
AATGAAGGGCCTGCCG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001739A_C01 KMC001739A_c01
         (616 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T06391 isoprenylated protein - soybean (fragment) gi|532701...   141  6e-33
pir||T09338 DnaJ-like protein MsJ1 - alfalfa gi|2370312|emb|CAA0...   130  1e-29
emb|CAC39071.1| DnaJ-like protein [Oryza sativa]                      127  9e-29
pir||T43929 DnaJ protein homolog [imported] - Salix gilgiana gi|...   120  1e-26
pir||T01643 DnaJ protein homolog ZMDJ1 - maize gi|2984709|gb|AAC...   117  1e-25

>pir||T06391 isoprenylated protein - soybean (fragment) gi|532701|gb|AAA65011.1|
           similar to Atriplex nummularia chaperone ANJ1 protein,
           Swiss-Prot Accession Number JQ2142
          Length = 86

 Score =  141 bits (356), Expect = 6e-33
 Identities = 72/89 (80%), Positives = 80/89 (88%), Gaps = 1/89 (1%)
 Frame = -2

Query: 591 LYIKFNVDFPDSGFISPDQCQLLEKILPQKSSKK-ADMELDDCEETILHDVNNLEEEMRR 415
           LYI+FNVDFPDSGF+SPDQCQLLEK+LPQKSSK  +DMELDDCEET LHDV N +EEMRR
Sbjct: 1   LYIQFNVDFPDSGFLSPDQCQLLEKVLPQKSSKHVSDMELDDCEETTLHDV-NFKEEMRR 59

Query: 414 RKQQRSREAYDEDEDDDEPSTQRVQCAQQ 328
           ++QQ+ REAY  DEDDDEPS QRVQCAQQ
Sbjct: 60  KQQQQYREAY--DEDDDEPSGQRVQCAQQ 86

>pir||T09338 DnaJ-like protein MsJ1 - alfalfa gi|2370312|emb|CAA04447.1|
           DnaJ-like protein [Medicago sativa]
           gi|3202020|gb|AAC19391.1| DnaJ-like protein MsJ1
           [Medicago sativa]
          Length = 423

 Score =  130 bits (327), Expect = 1e-29
 Identities = 70/98 (71%), Positives = 80/98 (81%), Gaps = 2/98 (2%)
 Frame = -2

Query: 615 GRPFIKGRLYIKFNVDFPDSGFISPDQCQLLEKILPQKSSKK-ADMELDDCEETILHDVN 439
           GRPF+KGRLYIKF+VDFPDSGF+SP Q   LEKILPQK+SK  +  E+DDCEET LHDV 
Sbjct: 328 GRPFMKGRLYIKFSVDFPDSGFLSPSQSLELEKILPQKTSKNLSQKEVDDCEETTLHDV- 386

Query: 438 NLEEEMRRRKQQRSREAYDEDED-DDEPSTQRVQCAQQ 328
           N+ EEM R+KQQ  REAYD+D+D DDE S  RVQCAQQ
Sbjct: 387 NIAEEMSRKKQQ-YREAYDDDDDEDDEHSQPRVQCAQQ 423

>emb|CAC39071.1| DnaJ-like protein [Oryza sativa]
          Length = 420

 Score =  127 bits (320), Expect = 9e-29
 Identities = 60/97 (61%), Positives = 80/97 (81%), Gaps = 1/97 (1%)
 Frame = -2

Query: 615 GRPFIKGRLYIKFNVDFPDSGFISPDQCQLLEKILPQK-SSKKADMELDDCEETILHDVN 439
           GRPF+KGRL+++FNV+FP+ G ++P QC+ LEKILP +  ++ +DMELD CEET +HDV 
Sbjct: 325 GRPFMKGRLFVEFNVEFPEPGALTPGQCRSLEKILPPRPRNQLSDMELDQCEETTMHDV- 383

Query: 438 NLEEEMRRRKQQRSREAYDEDEDDDEPSTQRVQCAQQ 328
           N+EEEMRRR+Q R +EAYDED+D+D  +  RVQCAQQ
Sbjct: 384 NIEEEMRRRQQHRRQEAYDEDDDEDAGAGPRVQCAQQ 420

>pir||T43929 DnaJ protein homolog [imported] - Salix gilgiana
           gi|4589726|dbj|BAA76883.1| DnaJ homolog protein [Salix
           gilgiana] gi|4589739|dbj|BAA76888.1| DnaJ homolog
           protein [Salix gilgiana]
          Length = 423

 Score =  120 bits (302), Expect = 1e-26
 Identities = 58/96 (60%), Positives = 77/96 (79%), Gaps = 1/96 (1%)
 Frame = -2

Query: 612 RPFIKGRLYIKFNVDFPDSGFISPDQCQLLEKILPQKSSKK-ADMELDDCEETILHDVNN 436
           RPF++G+LYI FNV FPDSG +SP+QC+ LE ILP + SK  ++ME+D+CEETI+HDVN 
Sbjct: 329 RPFMRGKLYIHFNVVFPDSGTLSPEQCRTLETILPPRQSKNLSEMEIDNCEETIMHDVNM 388

Query: 435 LEEEMRRRKQQRSREAYDEDEDDDEPSTQRVQCAQQ 328
            EE+ R+++Q+   EAYDEDE ++E S  RVQCAQQ
Sbjct: 389 EEEKRRKQQQRHQHEAYDEDE-EEESSMPRVQCAQQ 423

>pir||T01643 DnaJ protein homolog ZMDJ1 - maize gi|2984709|gb|AAC08009.1|
           DnaJ-related protein ZMDJ1 [Zea mays]
          Length = 419

 Score =  117 bits (293), Expect = 1e-25
 Identities = 57/96 (59%), Positives = 76/96 (78%), Gaps = 1/96 (1%)
 Frame = -2

Query: 612 RPFIKGRLYIKFNVDFPDSGFISPDQCQLLEKILPQK-SSKKADMELDDCEETILHDVNN 436
           RPF+KG+LYI F V+FPDS  ++P+QC+ LE +LP + SSK  DME+D+CEET +HDVNN
Sbjct: 327 RPFMKGKLYIHFTVEFPDS--LAPEQCKALETVLPPRPSSKLTDMEIDECEETTMHDVNN 384

Query: 435 LEEEMRRRKQQRSREAYDEDEDDDEPSTQRVQCAQQ 328
           +EEEMRR++   ++EAY+ED D+     QRVQCAQQ
Sbjct: 385 IEEEMRRKQAHAAQEAYEED-DEMPGGAQRVQCAQQ 419

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 507,728,674
Number of Sequences: 1393205
Number of extensions: 10745870
Number of successful extensions: 63771
Number of sequences better than 10.0: 187
Number of HSP's better than 10.0 without gapping: 46151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 57756
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24854530794
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD022c11_f BP045722 1 471 2 MF004f04_f BP028453 25 555 3 MPD064h01_f AV774294 26 531 4 MWL049c02_f AV769404 27 419 5 GNf081h02 BP073378 47 481 6 GENf021b03 BP059242 56 234 7 MPD087c02_f AV775703 56 583 8 MR062a12_f BP080719 59 450 9 MWM058e04_f AV765615 61 238 10 SPD087d08_f BP050945 62 494 11 MPDL074a05_f AV780283 92 582 12 MPD069d12_f AV774573 92 228 13 MF080h09_f BP032540 94 610 14 GENf012e08 BP058860 100 467 15 MF043b02_f BP030535 104 628

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