>KMC001724A_C02 KMC001724A_c02
tGGAGAGAAAATAAAGTCACTTAACAGTGATTATTATCATCATAAATATCACAATTCACA
AATGAAAGTGACAGTTTTCGAAAATGTAAGAAAATCTTAGTGATGGTGGCTGAACTCTTT
CTAAAAATTTGGGAAGATGAAACGATTTGAAACCATAGACGACGTACTCTGCAATTATTT
AGGTTGTGTCCGCCCAAACAGGCGCACGGGTCTTAGTCTAGTTGTAAGCTGAAGTTGACA
ACTTTAGCTACAACAGGATGGTAACAATGTGGATGGATCACTGGATCCAGTGTAACTCCA
CAACGAAACTTTCTAGTTTTATGAGACCTGATGGAGGGCATTAATCAACGTGACACTGGC
TGCATTTTTTCAGCTTCATCACGAAGCTCTTGGAACAGGACAGATGACAGGTATCTTTCC
CCAAAACTTGGATGAATAGTCACAATAAGTTTGCCTTTGTTCTCAGGCAGACTAGCCAAT
CTGAGTGCTGCAACTGTGTTAGCTCCAGAAGATATCCCAAGCATGAGTCCTTCCTTCAAG
GCCAATATCCTAGCCATTTTAACTGCATCTTCACTGCTTACTTCAAGAACTTTATCCATT
ACATCCATGTCCAATATGTCTGGTTTGAATCCAACACCATTGCCAGTTATATGATGAGGA
CCAGGTTTACCACCATTCAGCACATTACTTTCAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001724A_C02 KMC001724A_c02
         (694 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_191703.1| cysteine synthase AtcysC1; protein id: At3g6144...   196  2e-49
dbj|BAB18760.1| beta-cyanoalanine synthase [Solanum tuberosum]        191  8e-48
dbj|BAB20032.1| beta-cyanoalanine synthase like protein [Solanum...   191  8e-48
pir||A55450 cysteine synthase (EC 4.2.99.8) C precursor, mitocho...   191  8e-48
emb|CAC09469.1| cysteine synthase [Oryza sativa (indica cultivar...   182  3e-45

>ref|NP_191703.1| cysteine synthase AtcysC1; protein id: At3g61440.1, supported by
           cDNA: 32851., supported by cDNA: gi_20260389, supported
           by cDNA: gi_4996615 [Arabidopsis thaliana]
           gi|11269071|pir||T47936 cysteine synthase (EC 4.2.99.8)
           cysC1 [similarity] - Arabidopsis thaliana
           gi|4996616|dbj|BAA78560.1| cysteine synthase
           [Arabidopsis thaliana] gi|5824334|emb|CAB54830.1|
           cysteine synthase [Arabidopsis thaliana]
           gi|6850835|emb|CAB71074.1| cysteine synthase AtcysC1
           [Arabidopsis thaliana] gi|20260390|gb|AAM13093.1|
           cysteine synthase AtcysC1 [Arabidopsis thaliana]
           gi|21592815|gb|AAM64764.1| cysteine synthase AtcysC1
           [Arabidopsis thaliana] gi|22136208|gb|AAM91182.1|
           cysteine synthase AtcysC1 [Arabidopsis thaliana]
          Length = 368

 Score =  196 bits (499), Expect = 2e-49
 Identities = 98/114 (85%), Positives = 107/114 (92%)
 Frame = -3

Query: 692 ESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVKMARILALKEGLMLGI 513
           ESN+LNGGKPGPH ITGNGVGFKP+ILDMDVM+ VLEVSSEDA+KMAR LALKEGLM+GI
Sbjct: 253 ESNILNGGKPGPHAITGNGVGFKPEILDMDVMESVLEVSSEDAIKMARELALKEGLMVGI 312

Query: 512 SSGANTVAALRLASLPENKGKLIVTIHPSFGERYLSSVLFQELRDEAEKMQPVS 351
           SSGANTVAA+RLA +PENKGKLIVTIH SFGERYLSSVLF ELR EAE+M+PVS
Sbjct: 313 SSGANTVAAIRLAKMPENKGKLIVTIHASFGERYLSSVLFDELRKEAEEMKPVS 366

>dbj|BAB18760.1| beta-cyanoalanine synthase [Solanum tuberosum]
          Length = 351

 Score =  191 bits (485), Expect = 8e-48
 Identities = 95/114 (83%), Positives = 106/114 (92%)
 Frame = -3

Query: 692 ESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVKMARILALKEGLMLGI 513
           ESNVLNGG PGPH ITGNGVGFKPDILDMDVM++VL VSSE++V MAR LALKEGLM+GI
Sbjct: 236 ESNVLNGGNPGPHEITGNGVGFKPDILDMDVMEEVLMVSSEESVNMARELALKEGLMVGI 295

Query: 512 SSGANTVAALRLASLPENKGKLIVTIHPSFGERYLSSVLFQELRDEAEKMQPVS 351
           SSGANTVAALRLA+ PENKGKLIVTIHPSFGERYLSSVL++++R EA+ MQPVS
Sbjct: 296 SSGANTVAALRLANRPENKGKLIVTIHPSFGERYLSSVLYEDIRKEAQNMQPVS 349

>dbj|BAB20032.1| beta-cyanoalanine synthase like protein [Solanum tuberosum]
          Length = 347

 Score =  191 bits (485), Expect = 8e-48
 Identities = 95/114 (83%), Positives = 105/114 (91%)
 Frame = -3

Query: 692 ESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVKMARILALKEGLMLGI 513
           ESN+LNGGKPGPHHITGNGVG KPDI+DMD+M++VL VSSEDAV MAR LA+KEGLM+GI
Sbjct: 232 ESNILNGGKPGPHHITGNGVGSKPDIVDMDLMEEVLMVSSEDAVNMARELAVKEGLMVGI 291

Query: 512 SSGANTVAALRLASLPENKGKLIVTIHPSFGERYLSSVLFQELRDEAEKMQPVS 351
           SSGANTVAALRLA  PENKGKLIVT+H SFGERYLSSVL+Q+LR EAE MQPVS
Sbjct: 292 SSGANTVAALRLAQKPENKGKLIVTVHASFGERYLSSVLYQDLRKEAENMQPVS 345

>pir||A55450 cysteine synthase (EC 4.2.99.8) C precursor, mitochondrial -
           spinach gi|1066153|dbj|BAA07177.1| cysteine synthase
           [Spinacia oleracea]
          Length = 368

 Score =  191 bits (485), Expect = 8e-48
 Identities = 95/113 (84%), Positives = 104/113 (91%)
 Frame = -3

Query: 692 ESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVKMARILALKEGLMLGI 513
           ESN+LNGGKPGPH ITGNGVGFKP+ILDMDVMD VLEV S+DAVKMAR LAL+EGL++GI
Sbjct: 253 ESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMARQLALQEGLLVGI 312

Query: 512 SSGANTVAALRLASLPENKGKLIVTIHPSFGERYLSSVLFQELRDEAEKMQPV 354
           SSGANT+AAL LA  PENKGKLIVTIHPSFGERYLSS LF+ELR+EAE MQPV
Sbjct: 313 SSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAENMQPV 365

>emb|CAC09469.1| cysteine synthase [Oryza sativa (indica cultivar-group)]
          Length = 377

 Score =  182 bits (463), Expect = 3e-45
 Identities = 92/113 (81%), Positives = 101/113 (88%)
 Frame = -3

Query: 692 ESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVKMARILALKEGLMLGI 513
           E+NVLNGGKPGPH ITGNGVGFKP+IL+MD+M+KVLEV  EDAVKMAR LALKEGL++GI
Sbjct: 262 EANVLNGGKPGPHLITGNGVGFKPEILNMDIMEKVLEVKGEDAVKMARELALKEGLLVGI 321

Query: 512 SSGANTVAALRLASLPENKGKLIVTIHPSFGERYLSSVLFQELRDEAEKMQPV 354
           SSGANTVAAL LA  PENKGKLIVT+ PS GERYLSS LF+ELR EAE MQPV
Sbjct: 322 SSGANTVAALELAKKPENKGKLIVTVLPSLGERYLSSALFEELRAEAEAMQPV 374

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 590,007,577
Number of Sequences: 1393205
Number of extensions: 12253211
Number of successful extensions: 31988
Number of sequences better than 10.0: 364
Number of HSP's better than 10.0 without gapping: 30865
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31878
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31401661572
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD014c07_f AV770946 1 462 2 GNf038b03 BP070118 2 327 3 GNf036f01 BP069998 3 372 4 GNf023h08 BP069068 3 434 5 GNf094d06 BP074324 3 444 6 GNf084a01 BP073526 3 518 7 GNf090e02 BP074013 3 432 8 GENf036c12 BP059883 3 371 9 GENf048f03 BP060387 3 369 10 GNf076f12 BP073006 4 438 11 GNf041h04 BP070425 4 419 12 GENf091e03 BP062169 4 503 13 GENf019a05 BP059148 4 458 14 GENf026a08 BP059417 4 519 15 GENf049h02 BP060441 6 369 16 GENf061d02 BP060949 6 520 17 GNf075f07 BP072928 7 545 18 GENf011g06 BP058824 7 371 19 GENf092a11 BP062189 7 369 20 GNf089h07 BP073968 8 437 21 GNf015b12 BP068437 9 164 22 GNf070g01 BP072569 9 556 23 GENf086g07 BP062011 11 591 24 GNf082c12 BP073408 18 527 25 GNf088d06 BP073861 18 439 26 GENf064f12 BP061100 18 511 27 GENf078h06 BP061719 28 321 28 GNf052b01 BP071206 110 706

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