>KMC001697A_C02 KMC001697A_c02
GGAAGAATGGAGAAGCACTTTACATGTACTTTATTATGCAGAAGCAGTCCTCTGCAATTT
ACAACAATAGTTCAAAAAGCAATACGAATTTTCAACTACAACTCAATTCACAATAGCATT
TCCAAGAGGACGTTGGGGAAGAAGAAATCATATTGTAGAATTGTACCAATTAAAAACCAA
GTTCAAGTTGCCTTTATGGTAGCAACTTCCAAAATTCCTCTCCATAATTTTCTACGAAAC
ATCTAGCACCAACAGACTTATTCCAGTTACCATCAAAACATCCATTATGAAATGATATAA
GGAGTGAAAAAAACCAAGCTTAGTTTTTCTTGGCAACCCCGAGCCTGAGACGGAAATCCT
CTTCCATTAGAGGAAGGCCGTCGCGCAACTTGACCTTTGGTTCCCAGCCTAGCAACTCCT
TTGCTTTTGCTATGTCTGGTTTTCTCTGACGAGGATCATCGGGAGTGTTCTCAATCATCT
TTATCTCCACTGTTGAATTAATAAGCTCTTTCACATTCTCTGCAAGCTCAATCATTGTAA
ACTCACCTGGGTTCCCGATGTTGATTGGACCAGTGTTTTCCCCTTCCATTAGACGAATA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001697A_C02 KMC001697A_c02
         (599 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_190228.1| dTDP-glucose 4-6-dehydratases-like protein; pro...   165  5e-40
gb|AAB68605.1| thymidine diphospho-glucose 4-6-dehydratase homol...   165  5e-40
ref|NP_180443.1| putative nucleotide-sugar dehydratase; protein ...   164  6e-40
pir||T51252 dTDPglucose 4,6-dehydratase (EC 4.2.1.46) [imported]...   163  1e-39
ref|NP_200737.1| dTDP-glucose 4-6-dehydratase - like protein; pr...   162  2e-39

>ref|NP_190228.1| dTDP-glucose 4-6-dehydratases-like protein; protein id:
           At3g46440.1, supported by cDNA: 31901., supported by
           cDNA: gi_18175745, supported by cDNA: gi_20465506
           [Arabidopsis thaliana] gi|11270114|pir||T45701
           dTDP-glucose 4-6-dehydratases-like protein - Arabidopsis
           thaliana gi|6522623|emb|CAB62035.1| dTDP-glucose
           4-6-dehydratases-like protein [Arabidopsis thaliana]
           gi|18175746|gb|AAL59920.1| putative dTDP-glucose
           4-6-dehydratase [Arabidopsis thaliana]
           gi|20465507|gb|AAM20236.1| putative dTDP-glucose
           4-6-dehydratases [Arabidopsis thaliana]
           gi|21592727|gb|AAM64676.1| dTDP-glucose
           4-6-dehydratases-like protein [Arabidopsis thaliana]
          Length = 341

 Score =  165 bits (417), Expect = 5e-40
 Identities = 77/90 (85%), Positives = 86/90 (95%)
 Frame = -2

Query: 598 IRLMEGENTGPINIGNPGEFTMIELAENVKELINSTVEIKMIENTPDDPRQRKPDIAKAK 419
           +RLMEG++TGPINIGNPGEFTM+ELAE VKELIN ++EIKM+ENTPDDPRQRKPDI KAK
Sbjct: 251 MRLMEGDDTGPINIGNPGEFTMVELAETVKELINPSIEIKMVENTPDDPRQRKPDITKAK 310

Query: 418 ELLGWEPKVKLRDGLPLMEEDFRLRLGVAK 329
           E+LGWEPKVKLR+GLPLMEEDFRLRLGV K
Sbjct: 311 EVLGWEPKVKLREGLPLMEEDFRLRLGVHK 340

>gb|AAB68605.1| thymidine diphospho-glucose 4-6-dehydratase homolog [Prunus
           armeniaca]
          Length = 265

 Score =  165 bits (417), Expect = 5e-40
 Identities = 79/90 (87%), Positives = 84/90 (92%)
 Frame = -2

Query: 598 IRLMEGENTGPINIGNPGEFTMIELAENVKELINSTVEIKMIENTPDDPRQRKPDIAKAK 419
           IRLM+G+NTGPINIGNPGEFTMIELAENVKELIN  VEI M+ENTPDDPRQRKPDI KAK
Sbjct: 174 IRLMQGDNTGPINIGNPGEFTMIELAENVKELINPKVEIIMVENTPDDPRQRKPDITKAK 233

Query: 418 ELLGWEPKVKLRDGLPLMEEDFRLRLGVAK 329
           +LLGWEPKVKLRDGLPLME+DFR RLGV K
Sbjct: 234 DLLGWEPKVKLRDGLPLMEDDFRTRLGVPK 263

>ref|NP_180443.1| putative nucleotide-sugar dehydratase; protein id: At2g28760.1,
           supported by cDNA: gi_20466473 [Arabidopsis thaliana]
           gi|25292328|pir||F84688 probable nucleotide-sugar
           dehydratase [imported] - Arabidopsis thaliana
           gi|3927825|gb|AAC79582.1| putative nucleotide-sugar
           dehydratase [Arabidopsis thaliana]
           gi|20466474|gb|AAM20554.1| putative nucleotide-sugar
           dehydratase [Arabidopsis thaliana]
           gi|22136442|gb|AAM91299.1| putative nucleotide-sugar
           dehydratase [Arabidopsis thaliana]
          Length = 343

 Score =  164 bits (416), Expect = 6e-40
 Identities = 78/91 (85%), Positives = 85/91 (92%)
 Frame = -2

Query: 598 IRLMEGENTGPINIGNPGEFTMIELAENVKELINSTVEIKMIENTPDDPRQRKPDIAKAK 419
           +RLMEG+ TGPINIGNPGEFTM+ELAE VKELI   VEIKM+ENTPDDPRQRKPDI+KAK
Sbjct: 253 MRLMEGDQTGPINIGNPGEFTMVELAETVKELIKPDVEIKMVENTPDDPRQRKPDISKAK 312

Query: 418 ELLGWEPKVKLRDGLPLMEEDFRLRLGVAKK 326
           E+LGWEPKVKLR+GLPLMEEDFRLRLGV KK
Sbjct: 313 EVLGWEPKVKLREGLPLMEEDFRLRLGVPKK 343

>pir||T51252 dTDPglucose 4,6-dehydratase (EC 4.2.1.46) [imported] - chickpea
           gi|6469141|emb|CAB61752.1| dTDP-glucose 4-6-dehydratase
           [Cicer arietinum]
          Length = 346

 Score =  163 bits (413), Expect = 1e-39
 Identities = 79/92 (85%), Positives = 83/92 (89%)
 Frame = -2

Query: 598 IRLMEGENTGPINIGNPGEFTMIELAENVKELINSTVEIKMIENTPDDPRQRKPDIAKAK 419
           IRLM G +TGPIN+GNPGEFTM+ELAE VKELIN  VEIK +ENTPDDPRQRKPDI KAK
Sbjct: 255 IRLMGGSDTGPINLGNPGEFTMLELAETVKELINPNVEIKTVENTPDDPRQRKPDITKAK 314

Query: 418 ELLGWEPKVKLRDGLPLMEEDFRLRLGVAKKN 323
           ELLGWEPKVKLRDGLPLME DFRLRLGV KKN
Sbjct: 315 ELLGWEPKVKLRDGLPLMEGDFRLRLGVDKKN 346

>ref|NP_200737.1| dTDP-glucose 4-6-dehydratase - like protein; protein id:
           At5g59290.1, supported by cDNA: 6701., supported by
           cDNA: gi_14030702, supported by cDNA: gi_14595665,
           supported by cDNA: gi_20334715 [Arabidopsis thaliana]
           gi|9759250|dbj|BAB09774.1| dTDP-glucose 4-6-dehydratase
           [Arabidopsis thaliana]
           gi|14595666|gb|AAK70882.1|AF387789_1 UDP-glucuronic acid
           decarboxylase [Arabidopsis thaliana]
           gi|21594196|gb|AAM65979.1| dTDP-glucose
           4-6-dehydratase-like protein [Arabidopsis thaliana]
          Length = 342

 Score =  162 bits (411), Expect = 2e-39
 Identities = 76/90 (84%), Positives = 85/90 (94%)
 Frame = -2

Query: 598 IRLMEGENTGPINIGNPGEFTMIELAENVKELINSTVEIKMIENTPDDPRQRKPDIAKAK 419
           IRLMEG +TGPINIGNPGEFTM+ELAE VKELIN ++EIKM+ENTPDDPRQRKPDI+KAK
Sbjct: 252 IRLMEGNDTGPINIGNPGEFTMVELAETVKELINPSIEIKMVENTPDDPRQRKPDISKAK 311

Query: 418 ELLGWEPKVKLRDGLPLMEEDFRLRLGVAK 329
           E+LGWEPKVKLR+GLPLMEEDFRLRL V +
Sbjct: 312 EVLGWEPKVKLREGLPLMEEDFRLRLNVPR 341

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 507,243,659
Number of Sequences: 1393205
Number of extensions: 10839634
Number of successful extensions: 27932
Number of sequences better than 10.0: 307
Number of HSP's better than 10.0 without gapping: 26805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27842
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf051h02 BP071186 1 454 2 MR090b09_f BP082901 2 320 3 MFBL031d03_f BP042807 2 543 4 GNf062a04 BP071947 2 471 5 SPD087c12_f BP050939 3 487 6 MFB056c03_f BP038043 6 543 7 MR010h06_f BP076743 7 417 8 MR089c05_f BP082829 8 417 9 MFB051b10_f BP037682 9 510 10 MWL030e03_f AV769067 9 372 11 SPD077d02_f BP050155 9 620 12 MPDL027f06_f AV777861 10 557 13 SPD056c01_f BP048444 10 539

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