KMC001675A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC001675A_C01 KMC001675A_c01
ttctcattCTCTTCTTCTTCTTCTTCTTCCTTCAGATTCATTCTCATTTCCAATGGATCC
AGTGAAAGTATGGGGTAACACACCCCTAATCGAGGTCGACGGCGAGATCCACGACCTCAT
CGAGAAGGAGAAGCGCCGCCAGTGCCGCGGCATCGAGCTAATCGCATCGGAGAATTTCAC
CTCCTTCGCCGTCATCGAAGCTCTCGGCTCCGCCCTCACCAACAAATACTCCGAGGGCAT
GCCCGGTAACCGCTACTACGGCGGCAACGAGTTCATCGACCAGATCGAGAATCTCTGCCG
CTCTCGCGCGCTTCAAGCCTTCCATCTCGACGCTGCAAAATGGGGCGTTAACGTCCAGCC
CTACTCCGGCTCACCGGCCAACTTCGCCGCATACACCGCCGTGCTCAACCCTCACGACCG
CATCATGGGGTTGGATCTCCCCTCCGGTGGACATCTCACCCATGGGTACTATACCTCCGG
CGGGAAGAAGATCTCTGCGACTTCGATTTACTTTGAGAGTCTTCCGTATAAGGTGAACTC
CACCACCGGGTACATTGATTACGATCGGTTGGAGGAGAAGGCGTT
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001675A_C01 KMC001675A_c01
(585 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_193129.1| hydroxymethyltransferase; protein id: At4g13930... 335 3e-91
gb|AAM64493.1| hydroxymethyltransferase [Arabidopsis thaliana] 333 1e-90
gb|AAG40343.1|AF324991_1 AT4g13930 [Arabidopsis thaliana] 331 5e-90
ref|NP_193125.1| glycine hydroxymethyltransferase - like protein... 306 9e-83
ref|NP_564473.1| putative hydroxymethyltransferase; protein id: ... 254 6e-67
>ref|NP_193129.1| hydroxymethyltransferase; protein id: At4g13930.1, supported by
cDNA: 29662., supported by cDNA: gi_13358183, supported
by cDNA: gi_13605526, supported by cDNA: gi_20334773
[Arabidopsis thaliana] gi|7433539|pir||B71400 glycine
hydroxymethyltransferase (EC 2.1.2.1) - Arabidopsis
thaliana gi|2244749|emb|CAB10172.1|
hydroxymethyltransferase [Arabidopsis thaliana]
gi|7268097|emb|CAB78435.1| hydroxymethyltransferase
[Arabidopsis thaliana]
gi|13605527|gb|AAK32757.1|AF361589_1 AT4g13930/dl3005c
[Arabidopsis thaliana] gi|20334774|gb|AAM16248.1|
AT4g13930/dl3005c [Arabidopsis thaliana]
Length = 471
Score = 335 bits (858), Expect = 3e-91
Identities = 160/177 (90%), Positives = 167/177 (93%)
Frame = +2
Query: 53 MDPVKVWGNTPLIEVDGEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 232
M+PV WGNT L+ VD EIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1 MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
Query: 233 EGMPGNRYYGGNEFIDQIENLCRSRALQAFHLDAAKWGVNVQPYSGSPANFAAYTAVLNP 412
EG+PGNRYYGGNEFID+IENLCRSRAL+AFH D A WGVNVQPYSGSPANFAAYTA+L P
Sbjct: 61 EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120
Query: 413 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDRLEEKA 583
HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVN TTGYIDYD+LEEKA
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKA 177
>gb|AAM64493.1| hydroxymethyltransferase [Arabidopsis thaliana]
Length = 471
Score = 333 bits (853), Expect = 1e-90
Identities = 159/177 (89%), Positives = 166/177 (92%)
Frame = +2
Query: 53 MDPVKVWGNTPLIEVDGEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 232
M+PV WGNT L+ VD EIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1 MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
Query: 233 EGMPGNRYYGGNEFIDQIENLCRSRALQAFHLDAAKWGVNVQPYSGSPANFAAYTAVLNP 412
EG+PGNRYYGGNEFID+IENLCRSRAL+AFH D A WGVNVQPYSGSPANFAAYTA+L P
Sbjct: 61 EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120
Query: 413 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDRLEEKA 583
HDRIMGLDLPSGGHLTHGYYTSGGKK SATSIYFESLPYKVN TTGYIDYD+LEEKA
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKTSATSIYFESLPYKVNFTTGYIDYDKLEEKA 177
>gb|AAG40343.1|AF324991_1 AT4g13930 [Arabidopsis thaliana]
Length = 471
Score = 331 bits (848), Expect = 5e-90
Identities = 158/177 (89%), Positives = 165/177 (92%)
Frame = +2
Query: 53 MDPVKVWGNTPLIEVDGEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 232
M+PV WGNT L+ VD EIHDLIEKEKRRQCRGIELIASENFTSFAVIEALG ALTNKYS
Sbjct: 1 MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGRALTNKYS 60
Query: 233 EGMPGNRYYGGNEFIDQIENLCRSRALQAFHLDAAKWGVNVQPYSGSPANFAAYTAVLNP 412
EG+PGNRYYGGNEFID+IENLCR RAL+AFH D A WGVNVQPYSGSPANFAAYTA+L P
Sbjct: 61 EGIPGNRYYGGNEFIDEIENLCRPRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120
Query: 413 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDRLEEKA 583
HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVN TTGYIDYD+LEEKA
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKA 177
>ref|NP_193125.1| glycine hydroxymethyltransferase - like protein; protein id:
At4g13890.1 [Arabidopsis thaliana]
gi|7433549|pir||T05258 glycine hydroxymethyltransferase
(EC 2.1.2.1) F18A5.280 - Arabidopsis thaliana
gi|4455318|emb|CAB36853.1| glycine
hydroxymethyltransferase-like protein [Arabidopsis
thaliana] gi|7268093|emb|CAB78431.1| glycine
hydroxymethyltransferase-like protein [Arabidopsis
thaliana]
Length = 470
Score = 306 bits (785), Expect = 9e-83
Identities = 146/177 (82%), Positives = 161/177 (90%)
Frame = +2
Query: 53 MDPVKVWGNTPLIEVDGEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 232
M+PV WGNT L VD EI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1 MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60
Query: 233 EGMPGNRYYGGNEFIDQIENLCRSRALQAFHLDAAKWGVNVQPYSGSPANFAAYTAVLNP 412
EGMPGNRYYGG EFID+IE+LCRSR+L+AFH + KWGVNVQPYSGSPANFAAYTA+L P
Sbjct: 61 EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120
Query: 413 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDRLEEKA 583
HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKV+S TGYIDYD+LEEKA
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKA 177
>ref|NP_564473.1| putative hydroxymethyltransferase; protein id: At1g36370.1,
supported by cDNA: 122515. [Arabidopsis thaliana]
gi|27754227|gb|AAO22567.1| putative
hydroxymethyltransferase [Arabidopsis thaliana]
Length = 598
Score = 254 bits (649), Expect = 6e-67
Identities = 123/174 (70%), Positives = 143/174 (81%)
Frame = +2
Query: 62 VKVWGNTPLIEVDGEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 241
V+ WG+ P+ D +IH+L+EKEK+RQ RGIELIASENF AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189
Query: 242 PGNRYYGGNEFIDQIENLCRSRALQAFHLDAAKWGVNVQPYSGSPANFAAYTAVLNPHDR 421
PG RYY GN++IDQIENLC RAL AF L++ KWGVNVQPYS + ANFA YT +L P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249
Query: 422 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDRLEEKA 583
IMGLD PSGGH++HGY T GGKKISA SI+FES PYKVN TGYIDYD+LE+KA
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKA 303
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 591,700,920
Number of Sequences: 1393205
Number of extensions: 15161834
Number of successful extensions: 108814
Number of sequences better than 10.0: 2722
Number of HSP's better than 10.0 without gapping: 72591
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98722
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21997688174
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
|
|
|
|
|
|
clone |
accession |
position |
| 1 |
MWM102g05_f |
AV766392 |
1 |
565 |
| 2 |
MF071h10_f |
BP032095 |
9 |
406 |
| 3 |
MR010g09_f |
BP076734 |
40 |
149 |
| 4 |
GENf009c06 |
BP058713 |
223 |
587 |
|
Lotus japonicus
Kazusa DNA Research Institute