Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001658A_C01 KMC001658A_c01
(558 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pdb|1BYA| Beta-Amylase (E.C.3.2.1.2) gi|576034|pdb|1BYB| Beta... 166 2e-40
dbj|BAA09462.1| beta-amylase [Glycine max] gi|2196550|dbj|BAA204... 166 2e-40
sp|P10538|AMYB_SOYBN Beta-amylase (1,4-alpha-D-glucan maltohydro... 166 2e-40
pdb|1BFN| Beta-AmylaseBETA-Cyclodextrin Complex 164 9e-40
sp|O64407|AMYB_VIGUN BETA-AMYLASE (1,4-ALPHA-D-GLUCAN MALTOHYDRO... 164 9e-40
>pdb|1BYA| Beta-Amylase (E.C.3.2.1.2) gi|576034|pdb|1BYB| Beta-Amylase
(E.C.3.2.1.2) Reacted With 200 Mm Maltose And Complexed
With Maltotetraose gi|576035|pdb|1BYC| Beta-Amylase
(E.C.3.2.1.2) Reacted With 8 Mm Maltose And Complexed
With Maltotetraose gi|576036|pdb|1BYD| Beta-Amylase
(E.C.3.2.1.2) Reacted With 100 Mm Maltal And Complexed
With 2-Deoxymaltose
Length = 495
Score = 166 bits (420), Expect = 2e-40
Identities = 94/192 (48%), Positives = 117/192 (59%), Gaps = 7/192 (3%)
Frame = +1
Query: 4 QELFQLIQECGLKLQAIMSFHQCGGNVGDAVNIPIPQWVLDIGESNPDIFYTDISGTRNK 183
+ LFQL+QECGL LQAIMSFHQCGGNVGD VNIPIPQWVLDIGESN DIFYT+ SGTRNK
Sbjct: 73 RSLFQLVQECGLTLQAIMSFHQCGGNVGDIVNIPIPQWVLDIGESNHDIFYTNRSGTRNK 132
Query: 184 EYLGWVV*TTSVSSMEEQPLSYTVTT*RV-TEKTCQIS*NLN------LL*TLKLGLVQQ 342
EYL ++ +P+ + T + ++ N++ L+ +++GL
Sbjct: 133 EYL--------TVGVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPA 184
Query: 343 ESSDIPLIHKVGGGNILVLENVSAMTTM*RQISKRQRAGAGHPEWELPDDAGKYNDVPQS 522
P + G + + K A AGHPEWELPDDAGKYNDVP+S
Sbjct: 185 GELRYPSYPQSQGWEFPRIGEFQCYDKYLKADFKAAVARAGHPEWELPDDAGKYNDVPES 244
Query: 523 TEFFRSNGTYLT 558
T FF+SNGTY+T
Sbjct: 245 TGFFKSNGTYVT 256
Score = 157 bits (397), Expect = 8e-38
Identities = 73/86 (84%), Positives = 80/86 (92%)
Frame = +3
Query: 201 GVDNERIFHGRTAIELYSDYMKSYRENMSDFLKSELIIDIEVGLGPAGELRYPSYPQSRG 380
GVDNE IFHGRTAIE+YSDYMKS+RENMSDFL+S LIIDIEVGLGPAGELRYPSYPQS+G
Sbjct: 138 GVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPAGELRYPSYPQSQG 197
Query: 381 WEYPGIGECQCYDNYVKADFKAAASR 458
WE+P IGE QCYD Y+KADFKAA +R
Sbjct: 198 WEFPRIGEFQCYDKYLKADFKAAVAR 223
>dbj|BAA09462.1| beta-amylase [Glycine max] gi|2196550|dbj|BAA20453.1| beta-amylase
[Glycine max]
Length = 496
Score = 166 bits (420), Expect = 2e-40
Identities = 94/192 (48%), Positives = 117/192 (59%), Gaps = 7/192 (3%)
Frame = +1
Query: 4 QELFQLIQECGLKLQAIMSFHQCGGNVGDAVNIPIPQWVLDIGESNPDIFYTDISGTRNK 183
+ LFQL+QECGL LQAIMSFHQCGGNVGD VNIPIPQWVLDIGESN DIFYT+ SGTRNK
Sbjct: 74 RSLFQLVQECGLTLQAIMSFHQCGGNVGDIVNIPIPQWVLDIGESNHDIFYTNRSGTRNK 133
Query: 184 EYLGWVV*TTSVSSMEEQPLSYTVTT*RV-TEKTCQIS*NLN------LL*TLKLGLVQQ 342
EYL ++ +P+ + T + ++ N++ L+ +++GL
Sbjct: 134 EYL--------TVGVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPA 185
Query: 343 ESSDIPLIHKVGGGNILVLENVSAMTTM*RQISKRQRAGAGHPEWELPDDAGKYNDVPQS 522
P + G + + K A AGHPEWELPDDAGKYNDVP+S
Sbjct: 186 GELRYPSYPQSQGWEFPGIGEFQCYDKYLKADFKAAVARAGHPEWELPDDAGKYNDVPES 245
Query: 523 TEFFRSNGTYLT 558
T FF+SNGTY+T
Sbjct: 246 TGFFKSNGTYVT 257
Score = 160 bits (405), Expect = 1e-38
Identities = 74/86 (86%), Positives = 81/86 (94%)
Frame = +3
Query: 201 GVDNERIFHGRTAIELYSDYMKSYRENMSDFLKSELIIDIEVGLGPAGELRYPSYPQSRG 380
GVDNE IFHGRTAIE+YSDYMKS+RENMSDFL+S LIIDIEVGLGPAGELRYPSYPQS+G
Sbjct: 139 GVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPAGELRYPSYPQSQG 198
Query: 381 WEYPGIGECQCYDNYVKADFKAAASR 458
WE+PGIGE QCYD Y+KADFKAA +R
Sbjct: 199 WEFPGIGEFQCYDKYLKADFKAAVAR 224
>sp|P10538|AMYB_SOYBN Beta-amylase (1,4-alpha-D-glucan maltohydrolase)
gi|320571|pir||A60473 beta-amylase (EC 3.2.1.2) 2
[validated] - soybean gi|296447|emb|CAA50551.1| unnamed
protein product [Glycine max]
Length = 496
Score = 166 bits (420), Expect = 2e-40
Identities = 94/192 (48%), Positives = 117/192 (59%), Gaps = 7/192 (3%)
Frame = +1
Query: 4 QELFQLIQECGLKLQAIMSFHQCGGNVGDAVNIPIPQWVLDIGESNPDIFYTDISGTRNK 183
+ LFQL+QECGL LQAIMSFHQCGGNVGD VNIPIPQWVLDIGESN DIFYT+ SGTRNK
Sbjct: 74 RSLFQLVQECGLTLQAIMSFHQCGGNVGDIVNIPIPQWVLDIGESNHDIFYTNRSGTRNK 133
Query: 184 EYLGWVV*TTSVSSMEEQPLSYTVTT*RV-TEKTCQIS*NLN------LL*TLKLGLVQQ 342
EYL ++ +P+ + T + ++ N++ L+ +++GL
Sbjct: 134 EYL--------TVGVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPA 185
Query: 343 ESSDIPLIHKVGGGNILVLENVSAMTTM*RQISKRQRAGAGHPEWELPDDAGKYNDVPQS 522
P + G + + K A AGHPEWELPDDAGKYNDVP+S
Sbjct: 186 GELRYPSYPQSQGWEFPRIGEFQCYDKYLKADFKAAVARAGHPEWELPDDAGKYNDVPES 245
Query: 523 TEFFRSNGTYLT 558
T FF+SNGTY+T
Sbjct: 246 TGFFKSNGTYVT 257
Score = 157 bits (397), Expect = 8e-38
Identities = 73/86 (84%), Positives = 80/86 (92%)
Frame = +3
Query: 201 GVDNERIFHGRTAIELYSDYMKSYRENMSDFLKSELIIDIEVGLGPAGELRYPSYPQSRG 380
GVDNE IFHGRTAIE+YSDYMKS+RENMSDFL+S LIIDIEVGLGPAGELRYPSYPQS+G
Sbjct: 139 GVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPAGELRYPSYPQSQG 198
Query: 381 WEYPGIGECQCYDNYVKADFKAAASR 458
WE+P IGE QCYD Y+KADFKAA +R
Sbjct: 199 WEFPRIGEFQCYDKYLKADFKAAVAR 224
>pdb|1BFN| Beta-AmylaseBETA-Cyclodextrin Complex
Length = 495
Score = 164 bits (414), Expect = 9e-40
Identities = 93/192 (48%), Positives = 116/192 (59%), Gaps = 7/192 (3%)
Frame = +1
Query: 4 QELFQLIQECGLKLQAIMSFHQCGGNVGDAVNIPIPQWVLDIGESNPDIFYTDISGTRNK 183
+ L QL+QECGL LQAIMSFHQCGGNVGD VNIPIPQWVLDIGESN DIFYT+ SGTRNK
Sbjct: 73 RSLLQLVQECGLTLQAIMSFHQCGGNVGDIVNIPIPQWVLDIGESNHDIFYTNRSGTRNK 132
Query: 184 EYLGWVV*TTSVSSMEEQPLSYTVTT*RV-TEKTCQIS*NLN------LL*TLKLGLVQQ 342
EYL ++ +P+ + T + ++ N++ L+ +++GL
Sbjct: 133 EYL--------TVGVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPA 184
Query: 343 ESSDIPLIHKVGGGNILVLENVSAMTTM*RQISKRQRAGAGHPEWELPDDAGKYNDVPQS 522
P + G + + K A AGHPEWELPDDAGKYNDVP+S
Sbjct: 185 GELRYPSYPQSQGWEFPGIGEFQCYDKYLKADFKAAVARAGHPEWELPDDAGKYNDVPES 244
Query: 523 TEFFRSNGTYLT 558
T FF+SNGTY+T
Sbjct: 245 TGFFKSNGTYVT 256
Score = 160 bits (405), Expect = 1e-38
Identities = 74/86 (86%), Positives = 81/86 (94%)
Frame = +3
Query: 201 GVDNERIFHGRTAIELYSDYMKSYRENMSDFLKSELIIDIEVGLGPAGELRYPSYPQSRG 380
GVDNE IFHGRTAIE+YSDYMKS+RENMSDFL+S LIIDIEVGLGPAGELRYPSYPQS+G
Sbjct: 138 GVDNEPIFHGRTAIEIYSDYMKSFRENMSDFLESGLIIDIEVGLGPAGELRYPSYPQSQG 197
Query: 381 WEYPGIGECQCYDNYVKADFKAAASR 458
WE+PGIGE QCYD Y+KADFKAA +R
Sbjct: 198 WEFPGIGEFQCYDKYLKADFKAAVAR 223
>sp|O64407|AMYB_VIGUN BETA-AMYLASE (1,4-ALPHA-D-GLUCAN MALTOHYDROLASE)
gi|7435337|pir||T11575 beta-amylase (EC 3.2.1.2) -
cowpea gi|2995395|emb|CAA12395.1| beta amylase [Vigna
unguiculata]
Length = 496
Score = 164 bits (414), Expect = 9e-40
Identities = 93/192 (48%), Positives = 118/192 (61%), Gaps = 7/192 (3%)
Frame = +1
Query: 4 QELFQLIQECGLKLQAIMSFHQCGGNVGDAVNIPIPQWVLDIGESNPDIFYTDISGTRNK 183
+ LFQL+QECGLKLQAIMSFHQCGGNVGD VNIPIPQWVLDIGES+PDIFYT+ SGTR+K
Sbjct: 74 KSLFQLVQECGLKLQAIMSFHQCGGNVGDVVNIPIPQWVLDIGESDPDIFYTNRSGTRDK 133
Query: 184 EYLGWVV*TTSVSSMEEQPLSYTVTT*RV-TEKTCQIS*NLN------LL*TLKLGLVQQ 342
EYL ++ +P+ + T V ++ N++ ++ +++GL
Sbjct: 134 EYL--------TIGVDNKPIFHGRTAIEVYSDYMKSFRENMSDFLKSEVIIDIEVGLGPA 185
Query: 343 ESSDIPLIHKVGGGNILVLENVSAMTTM*RQISKRQRAGAGHPEWELPDDAGKYNDVPQS 522
P + G + + K A AGH EWELPDDAG YNDVP+S
Sbjct: 186 GELRYPSYPQNQGWVFPGIGEFQCYDKYLKAEFKAAAARAGHSEWELPDDAGTYNDVPES 245
Query: 523 TEFFRSNGTYLT 558
TEFF++NGTYLT
Sbjct: 246 TEFFKTNGTYLT 257
Score = 157 bits (398), Expect = 6e-38
Identities = 72/86 (83%), Positives = 82/86 (94%)
Frame = +3
Query: 201 GVDNERIFHGRTAIELYSDYMKSYRENMSDFLKSELIIDIEVGLGPAGELRYPSYPQSRG 380
GVDN+ IFHGRTAIE+YSDYMKS+RENMSDFLKSE+IIDIEVGLGPAGELRYPSYPQ++G
Sbjct: 139 GVDNKPIFHGRTAIEVYSDYMKSFRENMSDFLKSEVIIDIEVGLGPAGELRYPSYPQNQG 198
Query: 381 WEYPGIGECQCYDNYVKADFKAAASR 458
W +PGIGE QCYD Y+KA+FKAAA+R
Sbjct: 199 WVFPGIGEFQCYDKYLKAEFKAAAAR 224
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 528,480,669
Number of Sequences: 1393205
Number of extensions: 12089784
Number of successful extensions: 32803
Number of sequences better than 10.0: 90
Number of HSP's better than 10.0 without gapping: 31647
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32751
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
