>KMC001634A_C01 KMC001634A_c01
atcaactCAACTAAGAATAAGTTTTCATTATGTAAAGTTAATTGTTACACTCACACATCT
CATATAGCTGCCATCAGAAGAAGGTTCTCCCGGCACCCTATCCGGGTTTAATCACCAAAA
AAAACCAGGAACCCAATGTTATTTAATTGATTGGGAGTTTGAACCTGATTCAGGACTAAA
TAGGTACAAGCAAACATCCAATACTCCAATTAGAAATAAGACTTTGAATTTGATTCAAAG
GCGTTTACTTTCAGGAATGAAGTATCTGGATTTTATTATCGTCGTCATCGTACATGATCA
CGTACTCTACATTATGAAAGGAAGATACAAAAGTTAAAGATTTTCACATCTTTTTTTGAT
AGTGAAACTGAAATGGTGGTAGTACAAGCAAGACAGAAAAATTATTCAGGACCAAGCACC
CAAAAATTATCACGGCCAAGGCATCTGGAGAGACCCGTTCACTGATTGTAGAACCAAATT
GTGGGCCCCCTTTCCTATATGAAATAAGAAACTCAGTAGGGTATCCCTTCAGCTTCTGAG
GATTAATTCCAAGATCATTGAAAGTCAATGCATTTTCTGAAACAACAGTATCTGTAGTCA
AAGCACGGATCTGATCCAGATTGAATATGTTAGGAGTGGGGAGTGGAAAGGGGAGCTTAT
TAATTAATAATTCTCTCGGTGATGCCAATGCCTTAGCAATGGGAAAAGGAACCTTCACAT
ATCGAGGCCATTCTCGAATGACCTCATACATAAGCTCTGCCAATTCATGCATAGTATAGA
CCTCAGGACCCCCCAATTCATAAACCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001634A_C01 KMC001634A_c01
         (807 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||C84588 probable NADH-ubiquinone oxireductase [imported] - A...   196  3e-49
ref|NP_565469.1| putative NADH-ubiquinone oxireductase; protein ...   196  3e-49
ref|NP_422398.1| NADH-ubiquinone oxidoreductase 39 kDa subunit p...    64  3e-09
gb|ZP_00008063.1| hypothetical protein [Rhodobacter sphaeroides]       62  8e-09
ref|NP_767357.1| oxidoreductase [Bradyrhizobium japonicum] gi|27...    58  1e-07

>pir||C84588 probable NADH-ubiquinone oxireductase [imported] - Arabidopsis
           thaliana
          Length = 400

 Score =  196 bits (499), Expect = 3e-49
 Identities = 87/120 (72%), Positives = 106/120 (87%)
 Frame = -3

Query: 802 YELGGPEVYTMHELAELMYEVIREWPRYVKVPFPIAKALASPRELLINKLPFPLPTPNIF 623
           YELGGP+V+T HELAE+MY++IREWPRYVK+PFPIAKA+A+PR+ ++NK+PFPLP+P IF
Sbjct: 278 YELGGPDVFTTHELAEIMYDMIREWPRYVKLPFPIAKAMAAPRDFMVNKVPFPLPSPQIF 337

Query: 622 NLDQIRALTTDTVVSENALTFNDLGINPQKLKGYPTEFLISYRKGGPQFGSTISERVSPD 443
           NLDQI ALTTDT+VS+NAL F DL + P KLKGYP EFLI YRKGGP FGST+SE++  D
Sbjct: 338 NLDQINALTTDTLVSDNALKFQDLDLVPHKLKGYPVEFLIQYRKGGPNFGSTVSEKIPTD 397

>ref|NP_565469.1| putative NADH-ubiquinone oxireductase; protein id: At2g20360.1,
           supported by cDNA: 20429., supported by cDNA:
           gi_14334733, supported by cDNA: gi_15293276 [Arabidopsis
           thaliana] gi|14334734|gb|AAK59545.1| putative
           NADH-ubiquinone oxireductase [Arabidopsis thaliana]
           gi|15293277|gb|AAK93749.1| putative NADH-ubiquinone
           oxireductase [Arabidopsis thaliana]
           gi|20197857|gb|AAD21752.2| putative NADH-ubiquinone
           oxireductase [Arabidopsis thaliana]
          Length = 402

 Score =  196 bits (499), Expect = 3e-49
 Identities = 87/120 (72%), Positives = 106/120 (87%)
 Frame = -3

Query: 802 YELGGPEVYTMHELAELMYEVIREWPRYVKVPFPIAKALASPRELLINKLPFPLPTPNIF 623
           YELGGP+V+T HELAE+MY++IREWPRYVK+PFPIAKA+A+PR+ ++NK+PFPLP+P IF
Sbjct: 280 YELGGPDVFTTHELAEIMYDMIREWPRYVKLPFPIAKAMAAPRDFMVNKVPFPLPSPQIF 339

Query: 622 NLDQIRALTTDTVVSENALTFNDLGINPQKLKGYPTEFLISYRKGGPQFGSTISERVSPD 443
           NLDQI ALTTDT+VS+NAL F DL + P KLKGYP EFLI YRKGGP FGST+SE++  D
Sbjct: 340 NLDQINALTTDTLVSDNALKFQDLDLVPHKLKGYPVEFLIQYRKGGPNFGSTVSEKIPTD 399

>ref|NP_422398.1| NADH-ubiquinone oxidoreductase 39 kDa subunit precursor, putative
           [Caulobacter crescentus CB15] gi|25398051|pir||B87696
           hypothetical protein CC3604 [imported] - Caulobacter
           crescentus gi|13425350|gb|AAK25566.1| NADH-ubiquinone
           oxidoreductase 39 kDa subunit precursor, putative
           [Caulobacter crescentus CB15]
          Length = 319

 Score = 63.9 bits (154), Expect = 3e-09
 Identities = 39/113 (34%), Positives = 60/113 (52%), Gaps = 1/113 (0%)
 Frame = -3

Query: 802 YELGGPEVYTMHELAEL-MYEVIREWPRYVKVPFPIAKALASPRELLINKLPFPLPTPNI 626
           YELGGP VYTM E+ EL + E  R  P  + +P+P+A  + S  +L+   +P PL +   
Sbjct: 210 YELGGPTVYTMREILELILVETGRNRP-LIPLPWPVASLIGSLGDLVAGVIPPPLTS--- 265

Query: 625 FNLDQIRALTTDTVVSENALTFNDLGINPQKLKGYPTEFLISYRKGGPQFGST 467
              DQ++ L +D V  +      + G+ P  ++     +L  YRKGG Q+  T
Sbjct: 266 ---DQVQMLKSDNVAEQGLPGLAEAGVTPTAVEAIVPSYLYRYRKGG-QYAET 314

>gb|ZP_00008063.1| hypothetical protein [Rhodobacter sphaeroides]
          Length = 407

 Score = 62.4 bits (150), Expect = 8e-09
 Identities = 35/106 (33%), Positives = 54/106 (50%)
 Frame = -3

Query: 802 YELGGPEVYTMHELAELMYEVIREWPRYVKVPFPIAKALASPRELLINKLPFPLPTPNIF 623
           YELGGP+V T  +L +++  V+      V VPF +A  +    +L +    F L      
Sbjct: 288 YELGGPDVETFRDLMKMLLRVVERRKLIVNVPFWVANLIGGALDL-VQTATFGLLANKTL 346

Query: 622 NLDQIRALTTDTVVSENALTFNDLGINPQKLKGYPTEFLISYRKGG 485
             DQ+R L  D +V+ +A    DLGI+P  ++    E+L S+R  G
Sbjct: 347 TRDQVRNLRRDNIVAPDAKGLADLGISPTAMEAVLPEYLWSFRPSG 392

>ref|NP_767357.1| oxidoreductase [Bradyrhizobium japonicum]
           gi|27348966|dbj|BAC45982.1| oxidoreductase
           [Bradyrhizobium japonicum USDA 110]
          Length = 321

 Score = 58.2 bits (139), Expect = 1e-07
 Identities = 42/112 (37%), Positives = 54/112 (47%), Gaps = 6/112 (5%)
 Frame = -3

Query: 802 YELGGPEVYTMHELAELMYEVIREWPRYVKVPFPIAKALASPRELLINKLPFPLPTPNIF 623
           YELGGPEV TM E+ E + E+    P  V +PF +A+  A       N L F    P  F
Sbjct: 213 YELGGPEVLTMREIIEAILEITARKPTLVPLPFGLARFQA-------NFLQF---APGAF 262

Query: 622 NL--DQIRALTTDTVVSENA----LTFNDLGINPQKLKGYPTEFLISYRKGG 485
            L  DQ+  L  D VVS+ A    LT   LGI    L+    ++L  +R  G
Sbjct: 263 KLTPDQVTLLQRDNVVSDAAKAAGLTLEGLGITADSLEAIAPQYLWRFRAAG 314

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 704,154,173
Number of Sequences: 1393205
Number of extensions: 15688809
Number of successful extensions: 35031
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 33859
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35009
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 41176381974
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF043d11_f BP030557 1 554 2 SPD008a04_f BP044600 8 373 3 GENf063a05 BP061021 122 579 4 MR044d03_f BP079399 212 602 5 MR034a09_f BP078592 212 440 6 SPD092f02_f BP051357 212 397 7 MFB016b12_f BP035087 212 716 8 SPD098d01_f BP051819 213 738 9 MF068c02_f BP031909 215 639 10 MFB068c08_f BP038923 215 742 11 MF030h05_f BP029878 226 750 12 GENf064b08 BP061073 232 601 13 SPD028d05_f BP046213 232 616 14 MFB022f04_f BP035598 253 569 15 MF066c12_f BP031809 253 706 16 MFB022e09_f BP035592 257 733 17 MF032b01_f BP029951 257 819 18 GENf007e02 BP058633 258 562

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