Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001596A_C03 KMC001596A_c03
(698 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAN87572.1| gibberellin 2-oxidase 2 [Spinacia oleracea] 86 6e-18
ref|NP_175233.1| dioxygenase, putative; protein id: At1g47990.1 ... 85 6e-18
gb|AAD45424.1| gibberellin 2-oxidase-like protein [Pisum sativum] 84 2e-17
ref|NP_171742.1| dioxygenase, putative; protein id: At1g02400.1 ... 75 1e-15
ref|NP_177965.1| gibberellin 2- oxidase; protein id: At1g78440.1... 72 3e-14
>gb|AAN87572.1| gibberellin 2-oxidase 2 [Spinacia oleracea]
Length = 329
Score = 85.5 bits (210), Expect(2) = 6e-18
Identities = 41/72 (56%), Positives = 53/72 (72%)
Frame = -2
Query: 670 HLIPIHNSNNREDKSPPYNNKVGFGEHSDPQILTILRSNDVCGLQISLQEGVWIPVKPDP 491
H P+ N ++ + S ++GFGEHSDPQILTILRSNDV GLQI + +G+W+PV+PDP
Sbjct: 175 HYPPLKNKDSFINGS---GERIGFGEHSDPQILTILRSNDVGGLQICMPDGLWVPVQPDP 231
Query: 490 EAFCVNVGDVLE 455
AF V VGD L+
Sbjct: 232 NAFYVMVGDALQ 243
Score = 27.3 bits (59), Expect(2) = 6e-18
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -1
Query: 698 DSVLRLNHYPP 666
DS+LRLNHYPP
Sbjct: 168 DSLLRLNHYPP 178
>ref|NP_175233.1| dioxygenase, putative; protein id: At1g47990.1 [Arabidopsis
thaliana] gi|25285685|pir||C96520 probable dioxygenase,
65179-63000 [imported] - Arabidopsis thaliana
gi|12323091|gb|AAG51528.1|AC051631_8 dioxygenase,
putative; 65179-63000 [Arabidopsis thaliana]
Length = 321
Score = 85.1 bits (209), Expect(2) = 6e-18
Identities = 37/56 (66%), Positives = 46/56 (82%)
Frame = -2
Query: 610 KVGFGEHSDPQILTILRSNDVCGLQISLQEGVWIPVKPDPEAFCVNVGDVLEDNAN 443
+VGFGEH+DPQILT+LRSN V GLQ+S +G+W+ V PDP AFCVNVGD+L+ N
Sbjct: 187 RVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVMTN 242
Score = 27.7 bits (60), Expect(2) = 6e-18
Identities = 10/12 (83%), Positives = 12/12 (99%)
Frame = -1
Query: 698 DSVLRLNHYPPN 663
DSVLR+NHYPP+
Sbjct: 158 DSVLRVNHYPPS 169
>gb|AAD45424.1| gibberellin 2-oxidase-like protein [Pisum sativum]
Length = 345
Score = 84.3 bits (207), Expect(2) = 2e-17
Identities = 39/54 (72%), Positives = 43/54 (79%)
Frame = -2
Query: 616 NNKVGFGEHSDPQILTILRSNDVCGLQISLQEGVWIPVKPDPEAFCVNVGDVLE 455
NN +GFGEHSDPQILTILRSN+V GLQIS G+WIPV PDP F V VGD L+
Sbjct: 201 NNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPPDPSEFYVMVGDALQ 254
Score = 26.6 bits (57), Expect(2) = 2e-17
Identities = 9/11 (81%), Positives = 11/11 (99%)
Frame = -1
Query: 698 DSVLRLNHYPP 666
DS+LR+NHYPP
Sbjct: 172 DSLLRINHYPP 182
>ref|NP_171742.1| dioxygenase, putative; protein id: At1g02400.1 [Arabidopsis
thaliana] gi|25285706|pir||D86154 hypothetical protein
T6A9.9 [imported] - Arabidopsis thaliana
gi|9857536|gb|AAG00891.1|AC064879_9 Unknown protein
[Arabidopsis thaliana]
Length = 329
Score = 75.5 bits (184), Expect(2) = 1e-15
Identities = 34/56 (60%), Positives = 43/56 (76%)
Frame = -2
Query: 610 KVGFGEHSDPQILTILRSNDVCGLQISLQEGVWIPVKPDPEAFCVNVGDVLEDNAN 443
++GFGEHSDPQILT+LRSNDV GL+I ++G+WIP+ DP F V VGD L+ N
Sbjct: 197 QIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTN 252
Score = 29.6 bits (65), Expect(2) = 1e-15
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = -1
Query: 698 DSVLRLNHYPPNTY 657
DS+LRLNHYPP Y
Sbjct: 177 DSILRLNHYPPAPY 190
>ref|NP_177965.1| gibberellin 2- oxidase; protein id: At1g78440.1, supported by cDNA:
156482., supported by cDNA: gi_13430551 [Arabidopsis
thaliana] gi|25285703|pir||T52579 gibberellin
2beta-dioxygenase (EC 1.14.11.13) 1 [validated] -
Arabidopsis thaliana gi|4678366|emb|CAB41007.1| GA
2-oxidase [Arabidopsis thaliana]
gi|8052531|gb|AAF71795.1|AC013430_4 F3F9.5 [Arabidopsis
thaliana] gi|27754276|gb|AAO22591.1| putative
gibberellin 2- oxidase [Arabidopsis thaliana]
Length = 329
Score = 72.4 bits (176), Expect(2) = 3e-14
Identities = 37/82 (45%), Positives = 50/82 (60%)
Frame = -2
Query: 670 HLIPIHNSNNREDKSPPYNNKVGFGEHSDPQILTILRSNDVCGLQISLQEGVWIPVKPDP 491
H P SN + + N +GFGEH+DPQI+++LRSN+ GLQI+L +G WI V PD
Sbjct: 174 HYPPCPLSNKKTNGG---KNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDH 230
Query: 490 EAFCVNVGDVLEDNANANDEPV 425
+F NVGD L+ N + V
Sbjct: 231 TSFFFNVGDSLQVMTNGRFKSV 252
Score = 28.1 bits (61), Expect(2) = 3e-14
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -1
Query: 698 DSVLRLNHYPP 666
DS+LRLNHYPP
Sbjct: 167 DSILRLNHYPP 177
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 605,384,649
Number of Sequences: 1393205
Number of extensions: 13471113
Number of successful extensions: 33667
Number of sequences better than 10.0: 611
Number of HSP's better than 10.0 without gapping: 30941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33342
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31967457276
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
