>KMC001563A_C01 KMC001563A_c01
ggatgatggtGGAATACCACAAGTATGGTGTTAAATCCCTTATATCATTAGGATCAACCT
GGGTTATAAATATATTAGACCATGATTCTAATTAGCAAATGTGAAAATATAAGTTGTATC
CATTGAAAACTAGTTACAATTGGCTACAAGACCAGAAGAACAGTTTTTTTCCAGAAAATG
ATGAGCAGTGAAAGAAGCAAAACACAGCAATGGTCAAATGCTAGCTAGCTACTCATGCTG
CTCAAGAATTCTCTACTTATGGCTACTATGGTTATTAGCCGCCTGCAAATGTCACAACTC
CACACCTTATTTTCATGCAGAAGGCCCTCTGGCACTATTATTCACTTGCTCCACAGCTTC
TTGGTGGAGTCTGAACTTCTGGACCAACTTGTCCATTTAGGGCTTTTGCAGATTCATGGG
TTGGCAAAATTCTAACTTTAAGCAACATTGTCCCAGTGAAGGAATCCACCATGACCCCTA
GCATTTGTGAACTTATATGTGGCACCTGACACTTCATAGCCTTCAAGAACAGGATCATCT
GCAAGTAGAAGTGGTGTGTCTAGGTCAATGAACTTAAAACACCCAAGACCAGCAGCAAGG
TGACCAGCGAAACCCATAGCCAATCTTGTCTCAACCATACCACCAATCATCAAATCTAAT
CCTGCTACTTTCGCCTTATCTATAATATC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001563A_C01 KMC001563A_c01
         (689 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAB64152.1| putative muconate cycloisomerase-like protein [O...   136  2e-31
ref|NP_566605.1| expressed protein; protein id: At3g18270.1, sup...   127  1e-28
dbj|BAB01176.1| muconate cycloisomerase-like protein [Arabidopsi...   127  1e-28
gb|ZP_00018542.1| hypothetical protein [Chloroflexus aurantiacus]      75  7e-13
ref|NP_602402.1| O-succinylbenzoate-CoA synthase [Fusobacterium ...    47  2e-04

>dbj|BAB64152.1| putative muconate cycloisomerase-like protein [Oryza sativa
           (japonica cultivar-group)]
          Length = 530

 Score =  136 bits (343), Expect = 2e-31
 Identities = 63/81 (77%), Positives = 71/81 (86%)
 Frame = -1

Query: 689 DIIDKAKVAGLDLMIGGMVETRLAMGFAGHLAAGLGCFKFIDLDTPLLLADDPVLEGYEV 510
           ++ID A+ A + LMIGGMVETR+AMGFAGHLAAGLGCF F+DLDTPLLL++DPV  GYEV
Sbjct: 446 EVIDAARKARIALMIGGMVETRIAMGFAGHLAAGLGCFSFVDLDTPLLLSEDPVFGGYEV 505

Query: 509 SGATYKFTNARGHGGFLHWDN 447
           SG  YKFTNARGHGGFLH DN
Sbjct: 506 SGPVYKFTNARGHGGFLHLDN 526

>ref|NP_566605.1| expressed protein; protein id: At3g18270.1, supported by cDNA:
           gi_13878120, supported by cDNA: gi_17104792 [Arabidopsis
           thaliana] gi|13878121|gb|AAK44138.1|AF370323_1 unknown
           protein [Arabidopsis thaliana]
           gi|17104793|gb|AAL34285.1| unknown protein [Arabidopsis
           thaliana]
          Length = 410

 Score =  127 bits (319), Expect = 1e-28
 Identities = 57/83 (68%), Positives = 72/83 (86%)
 Frame = -1

Query: 689 DIIDKAKVAGLDLMIGGMVETRLAMGFAGHLAAGLGCFKFIDLDTPLLLADDPVLEGYEV 510
           ++I+ A+ +G++LMIGGMVETRLAMGF+GHLAAGLGCF+FIDLDTPLLLADDPV  GY+ 
Sbjct: 327 EVIELARSSGIELMIGGMVETRLAMGFSGHLAAGLGCFRFIDLDTPLLLADDPVQGGYKA 386

Query: 509 SGATYKFTNARGHGGFLHWDNVA 441
            GA Y+F +  GHGG+L W++VA
Sbjct: 387 CGAVYEFKDEGGHGGYLQWNDVA 409

>dbj|BAB01176.1| muconate cycloisomerase-like protein [Arabidopsis thaliana]
          Length = 415

 Score =  127 bits (319), Expect = 1e-28
 Identities = 57/83 (68%), Positives = 72/83 (86%)
 Frame = -1

Query: 689 DIIDKAKVAGLDLMIGGMVETRLAMGFAGHLAAGLGCFKFIDLDTPLLLADDPVLEGYEV 510
           ++I+ A+ +G++LMIGGMVETRLAMGF+GHLAAGLGCF+FIDLDTPLLLADDPV  GY+ 
Sbjct: 332 EVIELARSSGIELMIGGMVETRLAMGFSGHLAAGLGCFRFIDLDTPLLLADDPVQGGYKA 391

Query: 509 SGATYKFTNARGHGGFLHWDNVA 441
            GA Y+F +  GHGG+L W++VA
Sbjct: 392 CGAVYEFKDEGGHGGYLQWNDVA 414

>gb|ZP_00018542.1| hypothetical protein [Chloroflexus aurantiacus]
          Length = 362

 Score = 75.5 bits (184), Expect = 7e-13
 Identities = 39/70 (55%), Positives = 48/70 (67%), Gaps = 1/70 (1%)
 Frame = -1

Query: 674 AKVAGLDLMIGGMVETRLAMGFAGHLAAGLGCFKFIDLDTPLLLADDPVLEGYEVSGATY 495
           A+ AGL LMIGGMVE+ LAM  + H AAGLG F+F+DLDTPL LA +P   G+E+ G   
Sbjct: 284 ARAAGLGLMIGGMVESILAMTVSAHFAAGLGGFRFVDLDTPLFLATNPFQGGFELRGGVI 343

Query: 494 KFTN-ARGHG 468
              + A GHG
Sbjct: 344 DLCHIATGHG 353

>ref|NP_602402.1| O-succinylbenzoate-CoA synthase [Fusobacterium nucleatum subsp.
           nucleatum ATCC 25586] gi|19712800|gb|AAL93701.1|
           O-succinylbenzoate-CoA synthase [Fusobacterium nucleatum
           subsp. nucleatum ATCC 25586]
          Length = 375

 Score = 47.4 bits (111), Expect = 2e-04
 Identities = 22/56 (39%), Positives = 36/56 (64%)
 Frame = -1

Query: 686 IIDKAKVAGLDLMIGGMVETRLAMGFAGHLAAGLGCFKFIDLDTPLLLADDPVLEG 519
           II  A++ G++ MIG M+E ++++  A HLA        IDLD P+L ++DP++ G
Sbjct: 292 IISMAEIVGVECMIGCMLEAKVSVNAAVHLACAKQIITKIDLDGPVLCSEDPIIGG 347

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 598,476,651
Number of Sequences: 1393205
Number of extensions: 12769711
Number of successful extensions: 29604
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 28767
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29582
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31118763720
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENf017g11 BP059100 1 497 2 GNf063h07 BP072081 7 530 3 MPD086e05_f AV775650 21 525 4 MFB080h03_f BP039886 27 463 5 MR038d09_f BP078943 27 433 6 GENf098b09 BP062335 27 402 7 MFB017g10_f BP035211 29 586 8 GNf068d03 BP072407 35 429 9 GENf062e05 BP060994 50 325 10 GENf030h03 BP059634 63 441 11 MPD018e02_f AV771244 66 600 12 GENf054d01 BP060654 66 441 13 MF080c09_f BP032515 67 618 14 GNf048d05 BP070911 68 485 15 MR090b05_f BP082897 90 402 16 GENf004e10 BP058485 91 563 17 MFB044f10_f BP037237 94 629 18 GNf043e04 BP070537 94 570 19 MR051g07_f BP079967 94 501 20 MFB092h11_f BP040751 94 609 21 GENf036g02 BP059903 98 427 22 GNf098d05 BP074627 102 332 23 GENf071c05 BP061394 103 270 24 GENf056c10 BP060731 104 592 25 GENf043e02 BP060166 116 591 26 SPDL036f08_f BP054286 128 713 27 GENf019g10 BP059185 148 642

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