KMC001553A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC001553A_C01 KMC001553A_c01
ttttcagaacctcactttaagctactaaaataaattccactGGAAACACTAAAAAGAGCC
CCATTATCAAGAATCAAGTAAGGCTATACCGCCCAAGCAGATGGAAATTAAAATTAAAGA
ACTACATTAGAAAATTCAAAACCAAATATTACATGAAGTTCACATGGACAATAATAATAA
TAAGTTAAATCCAAAATTATAGAAAAGTAAAAAAAGAGTAGCAGAAAGCAGGGTGCTAGC
GAGATTTTCAGGGTACTACCTCAATCTGATTTCATCTCAATTTTCTTCAAGTTTAAGCGT
CGTCGTTTTTGTTGACTTTGGGTTTTCTGCAGAGGATCATATGCATCGGGGAGAAGATTC
CAGTGTCACCTCCTCTGGTCAAAAAGTCAGCGGTCTTGAACAGCATCTCATGAACATCCA
CCGTCCCCTTCGGCGCGATTCCCAATGCGGCGAGAACAGTCACCACAATGTGGTTCCTCC
AGTACGCGATCCTACCCATCTTCAACCGGTCCCACCACGGAAGCGCCGGTGGTTTCGCCA
GATCCAACTCCTTCACCACCTCGAACCCCACCTTACGCGCCGCCGCAGTGATGTCAGCGT
AGCTCCTCAGCCCTGGCAGCGCGTCGCCTCTCTCGATCCCCTGAATCACCTCCACGTGTT
CAGGATTCTCCGCCACGTACTTTTCCGTCGTGACCCACTCGTAGGAAACGTAGAGAGCCC
CCGGCTTCAGAACCCGGAAGATTTCCGCGTACACCTCCTCCAGCTTCGGCGCGTGGCATG
TCGCCTCGATCGAGTACGCGCCGTCGAATGTCTCATCGGAAAACGGCATCTGGAGGAAAT
TCCCACACACCACCTCGCAGAGAGAATCCAAACCCGCCTTCTTGTTGTGCTGCCTGGCCC
GATTCACCTGATACTCGTTGATCGTGATCCCCACGACGTTGGCTCGCGAGTGGGCGGCGA
TGGCACGCATGGGCCCACCAACTCCGCATCCCACATCGAGGATCTTGTGACCCGGCTTGG
CCTCGATTAGATCGACGGCATGCTCCTCGTGnAGGCGCGTGGCGTCGCGGTGAGATTTCC
CGGGGATGGAAGGGGAGAAATGGAAGGACTGGCCCCAACCCCACTCGTAGATGTCGGTGA
CGAGATTGTAGAAGGTGTCGACGAAATCGGGGACCTTCTCGGCGGTTTCGATCTCCTTGG
GGCGGCGGAAGAAGGACCAGTACTGGTCGTAGTTGTCCTGGACTTTCTCGGCGGAGATGG
ATCCGCCGGAGAGATCGGCGGCGCGTTTGCCCTTTTGTTCGGCGGGGCCGAGAACGCAGA
CGAACCAGTATAGGCCGCCGGCGATGAGAGCTCCGGTGCAGAAGAGAGAGAGGGAatcca
ttccaaaagagggaagggatgaagatcaaaaacagagaggagagaaagagagagatttct
caccctactttgggg
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001553A_C01 KMC001553A_c01
(1455 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_173458.1| sterol-C-methyltransferase; protein id: At1g203... 655 0.0
gb|AAM91592.1| 24-sterol C-methyltransferase [Arabidopsis thalia... 655 0.0
emb|CAA61966.1| sterol-C-methyltransferase [Arabidopsis thaliana... 654 0.0
gb|AAN31890.1| putative sterol-C-methyltransferase [Arabidopsis ... 652 0.0
gb|AAM63753.1| sterol-C-methyltransferase [Arabidopsis thaliana] 648 0.0
>ref|NP_173458.1| sterol-C-methyltransferase; protein id: At1g20330.1, supported by
cDNA: 2688., supported by cDNA: gi_12083241, supported by
cDNA: gi_15028254, supported by cDNA: gi_21281106
[Arabidopsis thaliana] gi|2129517|pir||S63686 sterol
24-C-methyltransferase (EC 2.1.1.41) - Arabidopsis
thaliana gi|9558593|gb|AAF88156.1|AC026234_7 Identical to
24-sterol C-methyltransferase from Arabidopsis thaliana
gi|2129517 and is a member of the ubiE/COQ5
methyltransferase family PF|01209. ESTs gb|T42228,
gb|T46520, gb|T41746, gb|N38458, gb|AI993515,
gb|AA389843, gb|AI099890, gb|AI099653 come from this gene
gi|12083242|gb|AAG48780.1|AF332417_1 putative
sterol-C-methyltransferase [Arabidopsis thaliana]
gi|15028255|gb|AAK76716.1| putative
sterol-C-methyltransferase [Arabidopsis thaliana]
gi|21281107|gb|AAM45009.1| putative
sterol-C-methyltransferase [Arabidopsis thaliana]
Length = 361
Score = 655 bits (1691), Expect = 0.0
Identities = 308/355 (86%), Positives = 334/355 (93%)
Frame = -3
Query: 1381 MDSLSLFCTGALIAGGLYWFVCVLGPAEQKGKRAADLSGGSISAEKVQDNYDQYWSFFRR 1202
MDSL+LF TGAL+A G+YWF+CVLGPAE+KGKRA DLSGGSISAEKVQDNY QYWSFFRR
Sbjct: 1 MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1201 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEHAVDLIE 1022
PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEE AVDLI+
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120
Query: 1021 AKPGHKILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARQHNKKAGLDSLCEVVCG 842
KPG KILDVGCGVGGPMRAIA+HSRANVVGITINEYQVNRAR HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180
Query: 841 NFLQMPFSDETFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKYVAENP 662
NFLQMPF D +FDGAYSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEK+ AE+
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240
Query: 661 EHVEVIQGIERGDALPGLRSYADITAAARKVGFEVVKELDLAKPPALPWWDRLKMGRIAY 482
EHVEVIQGIERGDALPGLR+Y DI A+KVGFE+VKE DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300
Query: 481 WRNHIVVTVLAALGIAPKGTVDVHEMLFKTADFLTRGGDTGIFSPMHMILCRKPK 317
WRNHIVV +L+A+G+APKGTVDVHEMLFKTAD+LTRGG+TGIFSPMHMILCRKP+
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPE 355
>gb|AAM91592.1| 24-sterol C-methyltransferase [Arabidopsis thaliana]
gi|25083682|gb|AAN72104.1| 24-sterol C-methyltransferase
[Arabidopsis thaliana]
Length = 361
Score = 655 bits (1689), Expect = 0.0
Identities = 308/355 (86%), Positives = 333/355 (93%)
Frame = -3
Query: 1381 MDSLSLFCTGALIAGGLYWFVCVLGPAEQKGKRAADLSGGSISAEKVQDNYDQYWSFFRR 1202
MDSL+LF TGAL+A G+YWF+CVLGPAE+KGKRA DLSGGSISAEKVQDNY QYWSFFRR
Sbjct: 1 MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1201 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEHAVDLIE 1022
PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEE AVDLI+
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120
Query: 1021 AKPGHKILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARQHNKKAGLDSLCEVVCG 842
KPG KILDVGCGVGGPMRAIA+HSRANVVGITINEYQVNRAR HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180
Query: 841 NFLQMPFSDETFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKYVAENP 662
NFLQMPF D +FDGAYSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEK+ AE+
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240
Query: 661 EHVEVIQGIERGDALPGLRSYADITAAARKVGFEVVKELDLAKPPALPWWDRLKMGRIAY 482
EHVEVIQGIERGDALPGLR+Y DI A+KVGFE+VKE DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300
Query: 481 WRNHIVVTVLAALGIAPKGTVDVHEMLFKTADFLTRGGDTGIFSPMHMILCRKPK 317
WRNHIVV +L+A+G+APKGTVDVHEMLFKTAD+LTRGG TGIFSPMHMILCRKP+
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGQTGIFSPMHMILCRKPE 355
>emb|CAA61966.1| sterol-C-methyltransferase [Arabidopsis thaliana]
gi|1587694|prf||2207220A sterol C-methyltransferase
Length = 361
Score = 654 bits (1686), Expect = 0.0
Identities = 308/355 (86%), Positives = 333/355 (93%)
Frame = -3
Query: 1381 MDSLSLFCTGALIAGGLYWFVCVLGPAEQKGKRAADLSGGSISAEKVQDNYDQYWSFFRR 1202
MDSL+LF TGAL+A G+YWF+CVLGPAE+KGKRA DLSGGSISAEKVQDNY QYWSFFRR
Sbjct: 1 MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1201 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEHAVDLIE 1022
PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEE AVDLI+
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120
Query: 1021 AKPGHKILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARQHNKKAGLDSLCEVVCG 842
KPG KILDVGCGVGGPMRAIA+HSRANVVGITINEYQVNRAR HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180
Query: 841 NFLQMPFSDETFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKYVAENP 662
NFLQMPF D +FDGAYSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEK+ AE+
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240
Query: 661 EHVEVIQGIERGDALPGLRSYADITAAARKVGFEVVKELDLAKPPALPWWDRLKMGRIAY 482
EHVEVIQGIERGDALPGLR+Y DI A+KVGFE+VKE DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300
Query: 481 WRNHIVVTVLAALGIAPKGTVDVHEMLFKTADFLTRGGDTGIFSPMHMILCRKPK 317
WRNHIVV +L+A+G+APKGTVDVHEMLFKTAD LTRGG+TGIFSPMHMILCRKP+
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADCLTRGGETGIFSPMHMILCRKPE 355
>gb|AAN31890.1| putative sterol-C-methyltransferase [Arabidopsis thaliana]
Length = 361
Score = 652 bits (1683), Expect = 0.0
Identities = 307/353 (86%), Positives = 332/353 (93%)
Frame = -3
Query: 1381 MDSLSLFCTGALIAGGLYWFVCVLGPAEQKGKRAADLSGGSISAEKVQDNYDQYWSFFRR 1202
MDSL+LF TGAL+A G+YWF+CVLGPAE+KGKRA DLSGGSISAEKVQDNY QYWSFFRR
Sbjct: 1 MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1201 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEHAVDLIE 1022
PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEE AVDLI+
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120
Query: 1021 AKPGHKILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARQHNKKAGLDSLCEVVCG 842
KPG KILDVGCGVGGPMRAIA+HSRANVVGITINEYQVNRAR HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180
Query: 841 NFLQMPFSDETFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKYVAENP 662
NFLQMPF D +FDGAYSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEK+ AE+
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240
Query: 661 EHVEVIQGIERGDALPGLRSYADITAAARKVGFEVVKELDLAKPPALPWWDRLKMGRIAY 482
EHVEVIQGIERGDALPGLR+Y DI A+KVGFE+VKE DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300
Query: 481 WRNHIVVTVLAALGIAPKGTVDVHEMLFKTADFLTRGGDTGIFSPMHMILCRK 323
WRNHIVV +L+A+G+APKGTVDVHEMLFKTAD+LTRGG+TGIFSPMHMILCRK
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRK 353
>gb|AAM63753.1| sterol-C-methyltransferase [Arabidopsis thaliana]
Length = 361
Score = 648 bits (1671), Expect = 0.0
Identities = 306/355 (86%), Positives = 332/355 (93%)
Frame = -3
Query: 1381 MDSLSLFCTGALIAGGLYWFVCVLGPAEQKGKRAADLSGGSISAEKVQDNYDQYWSFFRR 1202
MDSL+LF TGAL+A G+YWF+CVLGPAE+KGKRA DLSGGSISAEKVQDNY QYWSFFRR
Sbjct: 1 MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1201 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEHAVDLIE 1022
PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSH+DATRLHEE AVDLI+
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120
Query: 1021 AKPGHKILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARQHNKKAGLDSLCEVVCG 842
KPG KILDVG GVGG MRAIA+HSRANVVGITINEYQVNRAR HNKKAGLD+LCEVVCG
Sbjct: 121 VKPGQKILDVGXGVGGXMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180
Query: 841 NFLQMPFSDETFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKYVAENP 662
NFLQMPF D +FDGAYSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEK+ AE+
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240
Query: 661 EHVEVIQGIERGDALPGLRSYADITAAARKVGFEVVKELDLAKPPALPWWDRLKMGRIAY 482
EHVEVIQGIERGDALPGLR+Y DI A+KVGFE+VKE DLA PPA PWW RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300
Query: 481 WRNHIVVTVLAALGIAPKGTVDVHEMLFKTADFLTRGGDTGIFSPMHMILCRKPK 317
WRNHIVV +L+A+G+APKGTVDVHEMLFKTAD+LTRGG+TGIFSPMHMILCRKP+
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPE 355
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,446,804,165
Number of Sequences: 1393205
Number of extensions: 40130858
Number of successful extensions: 382566
Number of sequences better than 10.0: 6974
Number of HSP's better than 10.0 without gapping: 187942
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 312326
length of database: 448,689,247
effective HSP length: 127
effective length of database: 271,752,212
effective search space used: 97015539684
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
MFB097f06_f |
BP041078 |
1 |
242 |
| 2 |
MPD074h05_f |
AV774884 |
33 |
287 |
| 3 |
MPD085d08_f |
AV775579 |
66 |
584 |
| 4 |
MFB069g11_f |
BP039042 |
66 |
599 |
| 5 |
MR085f12_f |
BP082562 |
66 |
466 |
| 6 |
MFB045g07_f |
BP037311 |
68 |
400 |
| 7 |
MR037g04_f |
BP078887 |
70 |
143 |
| 8 |
MFB082c06_f |
BP039990 |
72 |
579 |
| 9 |
SPD039f09_f |
BP047120 |
78 |
666 |
| 10 |
SPD054e03_f |
BP048311 |
79 |
438 |
| 11 |
GENf004b12 |
BP058468 |
94 |
615 |
| 12 |
GNf008b05 |
BP067930 |
94 |
570 |
| 13 |
MPD033e04_f |
AV772260 |
96 |
253 |
| 14 |
MFBL051c04_f |
BP043871 |
99 |
552 |
| 15 |
GNf081f09 |
BP073363 |
103 |
595 |
| 16 |
SPD038e06_f |
BP047028 |
103 |
534 |
| 17 |
MFB015a09_f |
BP034999 |
103 |
656 |
| 18 |
MF012a12_f |
BP028845 |
103 |
314 |
| 19 |
GNf033b01 |
BP069743 |
106 |
547 |
| 20 |
MFB002c12_f |
BP034033 |
107 |
687 |
| 21 |
GNf070c12 |
BP072541 |
107 |
225 |
| 22 |
GNf041c12 |
BP070384 |
110 |
265 |
| 23 |
MF087c08_f |
BP032865 |
110 |
553 |
| 24 |
MPD099h09_f |
AV776494 |
110 |
227 |
| 25 |
MWM081f03_f |
AV766053 |
110 |
512 |
| 26 |
SPD042b11_f |
BP047323 |
110 |
662 |
| 27 |
MR040d03_f |
BP079088 |
110 |
498 |
| 28 |
MPD067e02_f |
AV774460 |
111 |
232 |
| 29 |
SPD058b06_f |
BP048593 |
114 |
692 |
| 30 |
GENf017b06 |
BP059068 |
114 |
477 |
| 31 |
MFBL053g10_f |
BP043986 |
114 |
666 |
| 32 |
MWM021f10_f |
AV764951 |
129 |
417 |
| 33 |
SPD098g09_f |
BP051854 |
141 |
649 |
| 34 |
SPD035d05_f |
BP046780 |
168 |
716 |
| 35 |
SPD049f05_f |
BP047924 |
496 |
1029 |
| 36 |
MWM052f02_f |
AV765519 |
896 |
1481 |
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Lotus japonicus
Kazusa DNA Research Institute