KMC001514A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC001514A_C01 KMC001514A_c01
atagcattgaaaattttcctCACATTAACATTAGGGCAGCTTTAAGCAAATATATTACAA
CAGTGACAAGGTAAGAAAATAAGCAGAGCAAACCCTAAAATAACAGATACATAATATAGC
TTCAAAACCCTATAGAAGCTAAGCTTTCATGTCAATGCACTTATACACAAGGAAACTGAA
TATCATGAAAATGGTTAATAACTTAATATTTCATTACATTTTCTATTGCACTACTCAACT
CTTAGCCACAGTGAAAATTGCCCCATTACATCAGATAAAAGAACGTATTTTTGGTGGCTT
AATACATCAATTCTCTCCGATCAGGCCCAATTATAACACTTTAGCTCTATGAAATCCTGG
TGAACATAGGGAGACCAATTTATAGTCTGAAAGTACTACTATAGCCTCCTCCTAGACCAG
AAGATCCATACCCATTGTAGGGTTGCGAGGGCAATGAAGACGACGGATACGATGAACTTC
CCAATTGAGAGTAAGAAGATCTCAGACCTGCATCTAACCTGCTGTAACTACTAGCGACAG
GTTCCTGGTGATGGGTTATAACATCCTGAATCAATTGTTGTGCTATTTGAACC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001514A_C01 KMC001514A_c01
         (593 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAG60186.1|AC084763_6 putative nucleic acid binding protein [...    73  3e-12
ref|NP_194330.1| KH domain protein; protein id: At4g26000.1, sup...    40  0.022
ref|NP_524390.2| Ecdysone-dependent gene 91 CG7539-PA gi|1738041...    35  0.94
pir||C49773 ecdysone-dependent cytokeratin-like protein EDG-91 -...    35  0.94
ref|NP_000413.1| keratin 17 [Homo sapiens] gi|547751|sp|Q04695|K...    34  1.6

>gb|AAG60186.1|AC084763_6 putative nucleic acid binding protein [Oryza sativa]
          Length = 458

 Score = 72.8 bits (177), Expect = 3e-12
 Identities = 42/72 (58%), Positives = 49/72 (67%), Gaps = 3/72 (4%)
 Frame = -2

Query: 592 VQIAQQLIQDVITHHQEPVASSYSRLDAGLRSSYSQLGSSSYPSSSLP---SQPYNGYGS 422
           VQ A QLIQD +  H++ V SSY+ LD   R SYSQ GSS+YPSSSLP   S    GY S
Sbjct: 387 VQAAYQLIQDSLAAHRDSVRSSYAGLDPVYRPSYSQYGSSTYPSSSLPSYSSMDGGGYSS 446

Query: 421 SGLGGGYSSTFR 386
           SGL GGY S++R
Sbjct: 447 SGL-GGYGSSYR 457

>ref|NP_194330.1| KH domain protein; protein id: At4g26000.1, supported by cDNA:
           33495., supported by cDNA: gi_15810498, supported by
           cDNA: gi_20466385, supported by cDNA: gi_21280914
           [Arabidopsis thaliana] gi|7485827|pir||T04255
           hypothetical protein F20B18.110 - Arabidopsis thaliana
           gi|4538929|emb|CAB39665.1| putative nucleic acid binding
           protein [Arabidopsis thaliana]
           gi|7269451|emb|CAB79455.1| putative nucleic acid binding
           protein [Arabidopsis thaliana]
           gi|15810499|gb|AAL07137.1| putative nucleic acid binding
           protein [Arabidopsis thaliana]
           gi|20466386|gb|AAM20510.1| putative nucleic acid binding
           protein [Arabidopsis thaliana]
           gi|21280915|gb|AAM45112.1| putative nucleic acid binding
           protein [Arabidopsis thaliana]
          Length = 495

 Score = 40.0 bits (92), Expect = 0.022
 Identities = 32/97 (32%), Positives = 45/97 (45%), Gaps = 31/97 (31%)
 Frame = -2

Query: 592 VQIAQQLIQDVITHHQEPVASS--YSRLDAGL---------------------------- 503
           VQ A+QLIQ+ I +H+EPV+ S  Y+R+D+G                             
Sbjct: 396 VQTAEQLIQEFIINHKEPVSVSGGYARIDSGYVPAYPPQLSNRQEPLPSTYMGTEPVQYR 455

Query: 502 RSSYSQLGSSSYPSSSLPSQPYNG-YGSSGLGGGYSS 395
            ++YSQLG  S  + +L  Q Y   Y  +   GGYSS
Sbjct: 456 PTAYSQLGGPSTYTPTLTGQTYGSEYRPASDVGGYSS 492

>ref|NP_524390.2| Ecdysone-dependent gene 91 CG7539-PA
           gi|17380419|sp|P27781|CUP9_DROME Pupal cuticle protein
           Edg-91 precursor (Ecdysone-dependent protein 91)
           gi|7300278|gb|AAF55440.1| CG7539-PA [Drosophila
           melanogaster]
          Length = 159

 Score = 34.7 bits (78), Expect = 0.94
 Identities = 17/40 (42%), Positives = 17/40 (42%), Gaps = 9/40 (22%)
 Frame = -2

Query: 493 YSQLGSSSYPSSSLPSQP---------YNGYGSSGLGGGY 401
           YS  G   YP       P         YNGYG SG GGGY
Sbjct: 55  YSGFGGGGYPGGYASGYPGGYGGGYSGYNGYGGSGFGGGY 94

>pir||C49773 ecdysone-dependent cytokeratin-like protein EDG-91 - fruit fly
           (Drosophila melanogaster) gi|157327|gb|AAA28502.1|
           cuticle protein gi|157329|gb|AAA28503.1| cuticle protein
          Length = 159

 Score = 34.7 bits (78), Expect = 0.94
 Identities = 17/40 (42%), Positives = 17/40 (42%), Gaps = 9/40 (22%)
 Frame = -2

Query: 493 YSQLGSSSYPSSSLPSQP---------YNGYGSSGLGGGY 401
           YS  G   YP       P         YNGYG SG GGGY
Sbjct: 55  YSGFGGGGYPGGYASGYPGGYGGGYSGYNGYGGSGFGGGY 94

>ref|NP_000413.1| keratin 17 [Homo sapiens] gi|547751|sp|Q04695|K1CQ_HUMAN Keratin,
           type I cytoskeletal 17 (Cytokeratin 17) (K17) (CK 17)
           (39.1) gi|422802|pir||S30433 keratin 17, type I,
           cytoskeletal - human gi|30379|emb|CAA79626.1|
           cytokeratin 17 [Homo sapiens] gi|34075|emb|CAA44451.1|
           keratin related product [Homo sapiens]
           gi|14198021|gb|AAH00159.2|AAH00159 keratin 17 [Homo
           sapiens] gi|15080273|gb|AAH11901.1|AAH11901 Similar to
           keratin 17 [Homo sapiens]
          Length = 432

 Score = 33.9 bits (76), Expect = 1.6
 Identities = 18/44 (40%), Positives = 24/44 (53%)
 Frame = -2

Query: 520 RLDAGLRSSYSQLGSSSYPSSSLPSQPYNGYGSSGLGGGYSSTF 389
           RL  GL +   +LGS+    S+L    Y+   S G GGGY S+F
Sbjct: 30  RLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSF 73

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 460,855,823
Number of Sequences: 1393205
Number of extensions: 9467461
Number of successful extensions: 24684
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 21909
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24271
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22854740960
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MFB078c06_f BP039684 1 515
2 MWM212a06_f AV767998 21 389
3 MFB045h02_f BP037317 71 198
4 SPD022f10_f BP045754 73 596
5 SPDL023g04_f BP053456 85 578
6 GENf002e04 BP058399 91 332
7 GENf080b08 BP061772 91 500
8 MFB028e04_f BP036050 97 511
9 MWM079b10_f AV765999 173 577




Lotus japonicus
Kazusa DNA Research Institute