>KMC001510A_C04 KMC001510A_c04
ctGTCAAACATATTTTTTATTTTATTTTTTCTCTTTAAACATGTAAATAGAAAATGAGCA
TATCAAACATACCCTTATTTTTATATTTCACTCCGGATATTTATACATATGGATTTTTAT
TCAACACTTTGCGTTTGTGACTCAGAGTGGCCTCATTGACCAGCAGATTTCTGCGAAGGG
AGTGAGCCTGTTATCAGCCAGCCTCAACTGGTACGCAGCTTTCTTATATTCTCCCCATGT
GAATTCTTTATACAAACTATCTTCTTCCTTTGACACGAGTGAAGGCAAAGGTGCTAATTT
TTCAGTCAAGGGTGCTCCTCCAAAGTAGATCATTGAAACTCTTGACATGGCCGTGTCACT
CAACACTCTGTGCTTTACACTTCTGAAGCTCCCATTGGTCATCACCTGCAAGGAGTCACC
AACATTGATGAAGAAAGAAGTCTGATCAGGTGGGATTGAAACCCAAGTACCATCTCTGAG
ACAGATTTGCAGTCCTGAAGTGTTGTTGGATCTCAGGACAGAAATCATCTGTGGGTCTGT
GTGCTCCCCAAACCCAATCAAATTTCTTCCACCAATTGC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001510A_C04 KMC001510A_c04
         (579 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAB41036.1| GA 2-oxidase [Phaseolus coccineus]                    219  2e-56
emb|CAC83090.1| gibberellin 2-oxidase [Cucurbita maxima]              214  6e-55
dbj|BAB12442.1| gibberellin 2-oxidase No1 [Lactuca sativa]            212  2e-54
emb|CAA70330.1| dioxygenase [Marah macrocarpus]                       212  3e-54
ref|NP_174296.1| unknown protein; protein id: At1g30040.1 [Arabi...   200  1e-50

>emb|CAB41036.1| GA 2-oxidase [Phaseolus coccineus]
          Length = 332

 Score =  219 bits (558), Expect = 2e-56
 Identities = 104/131 (79%), Positives = 117/131 (88%)
 Frame = -1

Query: 567 RNLIGFGEHTDPQMISVLRSNNTSGLQICLRDGTWVSIPPDQTSFFINVGDSLQVMTNGS 388
           RNL+GFGEHTDPQ+ISVLRSN+TSGLQICL DGTWVS+PPDQTSFFINVGD+LQVMTNG 
Sbjct: 196 RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGR 255

Query: 387 FRSVKHRVLSDTAMSRVSMIYFGGAPLTEKLAPLPSLVSKEEDSLYKEFTWGEYKKAAYQ 208
           F+SVKHRVL+DT  SR+SMIYFGG  L+E +APLPS++ K E+ LYKEFTW EYKKAAY 
Sbjct: 256 FKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAYT 315

Query: 207 LRLADNRLTPF 175
            RLADNRL PF
Sbjct: 316 SRLADNRLAPF 326

>emb|CAC83090.1| gibberellin 2-oxidase [Cucurbita maxima]
          Length = 321

 Score =  214 bits (545), Expect = 6e-55
 Identities = 103/141 (73%), Positives = 119/141 (84%)
 Frame = -1

Query: 579 AIGGRNLIGFGEHTDPQMISVLRSNNTSGLQICLRDGTWVSIPPDQTSFFINVGDSLQVM 400
           A+ G N+IGFGEHTDPQ+ISVLRSNNTSG QI L DG W+S+PPD +SFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 399 TNGSFRSVKHRVLSDTAMSRVSMIYFGGAPLTEKLAPLPSLVSKEEDSLYKEFTWGEYKK 220
           TNG F+SVKHRVL++++ SRVSMIYFGG PL+EK+APL SL+  EE SLYKEFTW EYK+
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 219 AAYQLRLADNRLTPFAEICWS 157
           +AY  RLADNRL PF  I  S
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

>dbj|BAB12442.1| gibberellin 2-oxidase No1 [Lactuca sativa]
          Length = 337

 Score =  212 bits (540), Expect = 2e-54
 Identities = 101/135 (74%), Positives = 118/135 (86%)
 Frame = -1

Query: 570 GRNLIGFGEHTDPQMISVLRSNNTSGLQICLRDGTWVSIPPDQTSFFINVGDSLQVMTNG 391
           GR L+GFGEHTDPQ+ISVLRSNNTSGL+I LRDG+W+S+P D  SFFINVGDSLQVMTNG
Sbjct: 200 GRKLVGFGEHTDPQIISVLRSNNTSGLEISLRDGSWMSVPADSDSFFINVGDSLQVMTNG 259

Query: 390 SFRSVKHRVLSDTAMSRVSMIYFGGAPLTEKLAPLPSLVSKEEDSLYKEFTWGEYKKAAY 211
            F+SVKHRV++++  SRVSMIYFGG PL+EK+APLPSL+  EEDSLYKEFTW EYKK+A+
Sbjct: 260 RFKSVKHRVVANSTKSRVSMIYFGGPPLSEKIAPLPSLIQGEEDSLYKEFTWFEYKKSAF 319

Query: 210 QLRLADNRLTPFAEI 166
             RLADNRL  F +I
Sbjct: 320 NTRLADNRLGLFEKI 334

>emb|CAA70330.1| dioxygenase [Marah macrocarpus]
          Length = 322

 Score =  212 bits (539), Expect = 3e-54
 Identities = 104/141 (73%), Positives = 120/141 (84%)
 Frame = -1

Query: 579 AIGGRNLIGFGEHTDPQMISVLRSNNTSGLQICLRDGTWVSIPPDQTSFFINVGDSLQVM 400
           A+ G N+IGFGEHTDPQ+ISVLRSNNTSGLQI L D  W+S+PPDQTSFFINVGDSLQVM
Sbjct: 183 ALEGTNMIGFGEHTDPQIISVLRSNNTSGLQISLPDANWISVPPDQTSFFINVGDSLQVM 242

Query: 399 TNGSFRSVKHRVLSDTAMSRVSMIYFGGAPLTEKLAPLPSLVSKEEDSLYKEFTWGEYKK 220
           TNG F+SVKHRVL+++  SR+SMIYFGG PL+EK+APLPSL+ K E+SLYKEFTW EYK+
Sbjct: 243 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM-KGEESLYKEFTWFEYKR 301

Query: 219 AAYQLRLADNRLTPFAEICWS 157
           +AY  RLADNRL  F  I  S
Sbjct: 302 SAYNSRLADNRLVHFERIAAS 322

>ref|NP_174296.1| unknown protein; protein id: At1g30040.1 [Arabidopsis thaliana]
           gi|25285702|pir||T52578 gibberellin 2beta-dioxygenase
           (EC 1.14.11.13) 2 [validated] - Arabidopsis thaliana
           gi|4678368|emb|CAB41008.1| GA 2-oxidase [Arabidopsis
           thaliana] gi|12324163|gb|AAG52050.1|AC022455_4 unknown
           protein; 59645-61446 [Arabidopsis thaliana]
           gi|27754705|gb|AAO22796.1| unknown protein [Arabidopsis
           thaliana] gi|28394101|gb|AAO42458.1| unknown protein
           [Arabidopsis thaliana]
          Length = 341

 Score =  200 bits (508), Expect = 1e-50
 Identities = 90/128 (70%), Positives = 113/128 (87%)
 Frame = -1

Query: 558 IGFGEHTDPQMISVLRSNNTSGLQICLRDGTWVSIPPDQTSFFINVGDSLQVMTNGSFRS 379
           +GFGEHTDPQ+ISVLRSNNT+GLQIC++DG+WV++PPD +SFFINVGD+LQVMTNG F+S
Sbjct: 202 VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKS 261

Query: 378 VKHRVLSDTAMSRVSMIYFGGAPLTEKLAPLPSLVSKEEDSLYKEFTWGEYKKAAYQLRL 199
           VKHRVL+DT  SR+SMIYFGG PL++K+APLP LV +++D LYKEFTW +YK +AY+ +L
Sbjct: 262 VKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKL 321

Query: 198 ADNRLTPF 175
            D RL  F
Sbjct: 322 GDYRLGLF 329

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 501,010,538
Number of Sequences: 1393205
Number of extensions: 10529469
Number of successful extensions: 25467
Number of sequences better than 10.0: 651
Number of HSP's better than 10.0 without gapping: 24417
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25147
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENf016b03 BP059019 1 511 2 GENf027g09 BP059505 3 377 3 GNf037a01 BP070027 19 338 4 GNf023b02 BP069014 30 153 5 GNf094g03 BP074354 45 453 6 GNf043a07 BP070504 47 407 7 GNf046a10 BP070733 49 466 8 GENf009a01 BP058700 49 422 9 GNf094h09 BP074367 49 532 10 GENf061d06 BP060951 49 559 11 GENf028e02 BP059536 49 477 12 GNf087h07 BP073819 49 444 13 GNf092d10 BP074168 56 585 14 GENf022a09 BP059279 57 409 15 GENf034b01 BP059781 57 420 16 GENf072d10 BP061439 62 499 17 GNf100b07 BP074757 62 588 18 GENf004h08 BP058504 62 532 19 GENf002d04 BP058393 63 433 20 GNf074c09 BP072830 64 125 21 GENf005e09 BP058538 68 433

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