>KMC001497A_C01 KMC001497A_c01
GATAAACACAAACTTCTTGCGAAAAGTCATGAAGAGTGTCTGTACATAAGCAGGGAATGC
AAATAAGATTCCCCACACACCAACTGAACTGGGGGAACCAAAAAGGCTAATGTATATATA
TAACATAAACCAAGTATTGAAGATTATACAACAAACCTTCAAAAAAAAAAGATTATACAA
CAAGCATGTACAACATATAAATTAATATGATAAATTTTGTGGGATCAGGTCTCCCCACAT
TTCTCTTTCCTCGATGCTGAGAAGTTCTTGAAGCTGAACATTAAGATGCTCTTCTTTCAG
CATGCTGTCTTCCAACTATAGATGATGATAAAATTATATGGCATACACTATCTCCCCACT
ACTATCACAATCAAGTTCAAAGTCGGAGTCTAAGTCATCCATGCCATCATCCATATCAAG
GTTATCAGCCATAAACTGATCTAAAGAATAAGACCCAGCTGCTGGTATAGTAGCTTCTGA
AATTATCTGCATGATCGTATCAATGCTCTGCATGGGCTGGTCTGGCTCTTCGAATTGGTT
ACTCATTATCTTTTGATCAGCAGGAAGATTCTTCAGAAACCATTCATTTTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001497A_C01 KMC001497A_c01
         (591 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF27340.1|AF186020_1 abscisic acid-activated protein kinase ...   127  1e-28
gb|AAM13097.1| putative protein [Arabidopsis thaliana] gi|250835...   120  2e-26
ref|NP_201489.1| protein kinase, 41K (EC 2.7.1.-) (pir||S71172);...   120  2e-26
ref|NP_190619.1| protein kinase SPK-2; protein id: At3g50500.1, ...   120  2e-26
gb|AAM65501.1| protein kinase SPK-2 [Arabidopsis thaliana]            120  2e-26

>gb|AAF27340.1|AF186020_1 abscisic acid-activated protein kinase [Vicia faba]
          Length = 349

 Score =  127 bits (318), Expect = 1e-28
 Identities = 63/88 (71%), Positives = 74/88 (83%), Gaps = 3/88 (3%)
 Frame = -1

Query: 591 KNEWFLKNLPAD---QKIMSNQFEEPDQPMQSIDTIMQIISEATIPAAGSYSLDQFMADN 421
           KNEWF KNLPAD   + IM NQFEEPDQPMQS+DTIMQIISEAT+PAAGSY  D+F    
Sbjct: 266 KNEWFRKNLPADLVNENIMDNQFEEPDQPMQSMDTIMQIISEATVPAAGSYYFDEF---- 321

Query: 420 LDMDDGMDDLDSDFELDCDSSGEIVYAI 337
           +++D+ MD++DSD+ELD DSSGEIVYAI
Sbjct: 322 IEVDEDMDEIDSDYELDVDSSGEIVYAI 349

>gb|AAM13097.1| putative protein [Arabidopsis thaliana] gi|25083584|gb|AAN72093.1|
           putative protein [Arabidopsis thaliana]
          Length = 349

 Score =  120 bits (300), Expect = 2e-26
 Identities = 60/87 (68%), Positives = 69/87 (78%), Gaps = 3/87 (3%)
 Frame = -1

Query: 588 NEWFLKNLPADQKIMSN---QFEEPDQPMQSIDTIMQIISEATIPAAGSYSLDQFMADNL 418
           + WFLKNLPAD    SN   QF+EP+QPMQS+DTIMQIISEATIPA  +  LD FM DNL
Sbjct: 263 HSWFLKNLPADLMNESNTGSQFQEPEQPMQSLDTIMQIISEATIPAVRNRCLDDFMTDNL 322

Query: 417 DMDDGMDDLDSDFELDCDSSGEIVYAI 337
           D+DD MDD DS+ E+D DSSGEIVYA+
Sbjct: 323 DLDDDMDDFDSESEIDIDSSGEIVYAL 349

>ref|NP_201489.1| protein kinase, 41K (EC 2.7.1.-) (pir||S71172); protein id:
           At5g66880.1, supported by cDNA: gi_166818 [Arabidopsis
           thaliana] gi|2146746|pir||S71172 protein kinase, 41K (EC
           2.7.1.-) - Arabidopsis thaliana gi|166819|gb|AAA32846.1|
           protein kinase gi|9758138|dbj|BAB08630.1| protein
           kinase, 41K (EC 2.7.1.-) [Arabidopsis thaliana]
          Length = 361

 Score =  120 bits (300), Expect = 2e-26
 Identities = 60/87 (68%), Positives = 69/87 (78%), Gaps = 3/87 (3%)
 Frame = -1

Query: 588 NEWFLKNLPADQKIMSN---QFEEPDQPMQSIDTIMQIISEATIPAAGSYSLDQFMADNL 418
           + WFLKNLPAD    SN   QF+EP+QPMQS+DTIMQIISEATIPA  +  LD FM DNL
Sbjct: 275 HSWFLKNLPADLMNESNTGSQFQEPEQPMQSLDTIMQIISEATIPAVRNRCLDDFMTDNL 334

Query: 417 DMDDGMDDLDSDFELDCDSSGEIVYAI 337
           D+DD MDD DS+ E+D DSSGEIVYA+
Sbjct: 335 DLDDDMDDFDSESEIDIDSSGEIVYAL 361

>ref|NP_190619.1| protein kinase SPK-2; protein id: At3g50500.1, supported by cDNA:
           3988., supported by cDNA: gi_16209651, supported by
           cDNA: gi_166816 [Arabidopsis thaliana]
           gi|1362002|pir||S56718 protein kinase SPK-2 (EC 2.7.1.-)
           - Arabidopsis thaliana gi|166817|gb|AAA32845.1| protein
           kinase gi|6561990|emb|CAB62479.1| protein kinase SPK-2
           [Arabidopsis thaliana]
          Length = 362

 Score =  120 bits (300), Expect = 2e-26
 Identities = 58/87 (66%), Positives = 70/87 (79%), Gaps = 3/87 (3%)
 Frame = -1

Query: 588 NEWFLKNLPAD---QKIMSNQFEEPDQPMQSIDTIMQIISEATIPAAGSYSLDQFMADNL 418
           ++WFLKNLP D   +  M +QF+EP+QPMQS+DTIMQIISEATIP   +  LD FMADNL
Sbjct: 276 DKWFLKNLPGDLMDENRMGSQFQEPEQPMQSLDTIMQIISEATIPTVRNRCLDDFMADNL 335

Query: 417 DMDDGMDDLDSDFELDCDSSGEIVYAI 337
           D+DD MDD DS+ E+D DSSGEIVYA+
Sbjct: 336 DLDDDMDDFDSESEIDVDSSGEIVYAL 362

>gb|AAM65501.1| protein kinase SPK-2 [Arabidopsis thaliana]
          Length = 362

 Score =  120 bits (300), Expect = 2e-26
 Identities = 58/87 (66%), Positives = 70/87 (79%), Gaps = 3/87 (3%)
 Frame = -1

Query: 588 NEWFLKNLPAD---QKIMSNQFEEPDQPMQSIDTIMQIISEATIPAAGSYSLDQFMADNL 418
           ++WFLKNLP D   +  M +QF+EP+QPMQS+DTIMQIISEATIP   +  LD FMADNL
Sbjct: 276 DKWFLKNLPGDLMDENRMGSQFQEPEQPMQSLDTIMQIISEATIPTVRNRCLDDFMADNL 335

Query: 417 DMDDGMDDLDSDFELDCDSSGEIVYAI 337
           D+DD MDD DS+ E+D DSSGEIVYA+
Sbjct: 336 DLDDDMDDFDSESEIDVDSSGEIVYAL 362

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 498,570,093
Number of Sequences: 1393205
Number of extensions: 10510781
Number of successful extensions: 28457
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 27016
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28364
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22569056698
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWM232b02_f AV768258 1 594 2 MR005g06_f BP076342 1 111 3 GENf002a07 BP058378 4 369

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